Glareosin : A novel sexually dimorphic urinary lipocalin in the bank vole, Myodes glareolus

Electronic supplementary material is available online at https://dx.doi.org/10.6084/m9.figshare.c.3859369. Detailed methods are presented in the electronic supplementary material. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [32] partner repository with the dataset identifier PXD006645 and 10.6019/PXD006645 This work was funded in part by BBSRC (BB/J002631/1 and BB/M012557/1).Peer reviewedPublisher PD

Propuesta para la intervención de los problemas familiares afectivos que contribuye a mejorar la relación trabajo-familia de los colaboradores de la empresa CRD Ingeniería s.a.s

El objetivo de esta investigación ha sido analizar la relación entre trabajo – familia e interacción laboral. Para llevar a cabo este estudio, se ha utilizado una muestra heterogénea de 45 participantes, donde el 80% son hombres y el 20% mujeres. Los instrumentos de recolección de datos fueron: Encuesta socio-demográfica y el Cuestionario de Interacción Trabajo-Familia (SWING). Los resultados reportaron una interacción positiva familia -trabajo, donde se evidencio bajas referencias de alteraciones a la salud; sin embargo, sí se registró una relación negativa de baja intensidad entre la relación trabajo-familia. Los resultados reportaron un ambiente de trabajo en buenas condiciones; interacción positiva familia-trabajo. La interacción trabajo-familia permite explicar la asociación entre factores como edad, horas trabajadas a la semana, sobrecarga, trabajo bajo presión y satisfacción con las condiciones de trabajo, sin embargo, se diseñó una propuesta de intervención para mejorar la flexibilidad de horarios y fortalecimiento de los mecanismos de afrontamiento de situaciones cotidianas en las que se pueden ver involucrados en la interacción familia – trabajo.The objective of this research has been to analyze the relationship between work - family and work interaction. To carry out this study, a heterogeneous sample of 45 participants was used, where 80% are men and the rest are women. The data collection instruments were: Socio-demographic survey and the Work-Family Interaction Questionnaire (SWING). The results reported a positive work-family interaction. Low references of alterations to health were evidenced; Nevertheless. There was a low intensity relationship between negative family-work interaction. The results reported a work environment in good condition; positive family-work interaction. The work-family interaction allows us to explain the association between factors such as age, hours worked per week, work overload, work under pressure and satisfaction with working conditions. However, an intervention proposal was designed to improve schedule flexibility and strengthen coping mechanisms in everyday situations in which they can be involved in family-work interaction.Índice de figuras v Índice de tablas. vi Título. 1 Problema de investigación. 1 Descripción del Problema: 1 Formulación del problema 3 Sistematización 3 Objetivos. 3 Objetivo General 3 Objetivos específicos 4 Justificación. 4 Estado del arte 5 Marco teórico 13 Factores psicosociales. 13 La Familia y su influencia en los entornos del individuo. 14 Conflicto Familia y trabajo. 18 Marco Legal 26 Marco Institucional 29 Reseña Histórica: “ 29 Marco metodológico de la investigación 31 Paradigma 31 Recolección de la información. 32 Población de Estudio. 32 Materiales. 33 Técnicas 39 Procedimientos 40 FASE 1. Revisión Bibliográfica 40 FASE 2. Aplicación del Instrumento 41 FASE 3. Análisis de Datos Recolectados 41 FASE 4. Diseño de Estrategias 41 Análisis estadístico 43 Resultados y propuesta de intervención 44 Respuesta al primer objetivo específico-Análisis e interpretación de los resultados. 44 Análisis de los resultados 82 Respuesta al segundo objetivo específico-Análisis e interpretación de los resultados. 84 Respuesta al tercer objetivo específico-Propuesta de intervención 88 Presupuesto 93 Discusión 93 Conclusiones y reflexiones finales 96 Recomendaciones 98 Referencias 102  EspecializaciónEspecialización en Gerencia de la Seguridad y Salud en el trabaj

Switching methods of self-harm at repeat episodes: Findings from a multicentre cohort study.

BACKGROUND: Self-poisoning and self-injury have widely differing incidences in hospitals and in the community, which has led to confusion about the concept of self-harm. Categorising self-harm simply by a method may be clinically misleading because many hospital-attending patients switch from one method of harm to another on subsequent episodes. The study set out to determine the frequency, pattern, determinants and characteristics of method-switching in self-harm episodes presenting to the general hospital. METHODS: The pattern of repeated self-harm was established from over 33,000 consecutive self-harm episodes in a multicentre English cohort, categorising self-harm methods as poisoning, cutting, other injury, and combined methods. RESULTS: Over an average of 30 months of follow-up, 23% of people repeated self-harm and one-third of them switched method, often rapidly, and especially where the person was male, younger, or had self-harmed previously. Self-poisoning was far less likely than other methods to lead on to switching. LIMITATIONS: Self-harm episodes that do not lead to hospital attendance are not included in these findings but people who self-harmed and went to hospital but were not admitted from the emergency department to the general hospital, or did not receive designated psychosocial assessment are included. People in the study were a mix of prevalent as well as incident cases. CONCLUSIONS: Method of self-harm is fluctuating and unpredictable. Clinicians should avoid false assumptions about people׳s risks or needs based simply on the method of harm

Reflections: Students\u27 Tribute to Stan Kuczaj (1950-2016)

On April 14th, 2016, Animal Behavior and Cognition lost its Editor-in-Chief. But the scientific community and the friends and colleagues of Stanley ‘Stan’ Kuczaj III lost so much more. As many know, Stan began his career in Developmental Psychology, making enormous contributions in the area of language development, but became best known for his many innovative contributions in the area of marine mammal behavior. Stan founded Animal Behavior and Cognition because he was deeply passionate about research with a broad range of topics concerning animal behavior, animal cognition, and animal welfare. He was equally passionate about the idea that science should be accessible to all, and that accessibility should not come at a financial burden to researchers. The current editorial team is committed to carrying on Stan’s vision for the journal, and we believe that its continuation will pay homage to Stan as a researcher, and as a leader within the scientific community. However, for the next few pages, we wish to pay special tribute to Stan as a mentor, as this role was perhaps the one that was most pivotal in defining who he was as a scientist, colleague, and friend. We take comfort in the fact that Stan’s memory will live on in the legacy of his mentees, many of whom became cherished friends and colleagues. Below you will find reflections from several of these former students who were given the difficult task of trying to summarize the most meaningful aspect of Stan’s influence on their personal and professional development. Although no few words could summarize the impact of someone as enigmatic as Stan, we hope that these reflections will contribute to a full and nuanced tribute to the man he was

Tracking the embryonic stem cell transition from ground state pluripotency

Mouse embryonic stem (ES) cells are locked into self-renewal by shielding from inductive cues. Release from this ground state in minimal conditions offers a system for delineating developmental progression from naive pluripotency. Here we examined the initial transition process. The ES cell population behaves asynchronously. We therefore exploited a short-half-life $\textit{Rex1::GFP}$ reporter to isolate cells either side of exit from naive status. Extinction of ES cell identity in single cells is acute. It occurs only after near-complete elimination of naïve pluripotency factors, but precedes appearance of lineage specification markers. Cells newly departed from the ES cell state display features of early post-implantation epiblast and are distinct from primed epiblast. They also exhibit a genome-wide increase in DNA methylation, intermediate between early and late epiblast. These findings are consistent with the proposition that naive cells transition to a distinct formative phase of pluripotency preparatory to lineage priming.This research was funded by the Wellcome Trust (091484/Z/10/Z and 095645/Z/11/Z), the Biotechnology and Biological Sciences Research Council (BB/M004023/1 and BB/K010867/1), a European Commission Framework 7 project EuroSyStem (HEALTH-F4-2007-200720 EUROSYSTEM), SysStemCell (ERC-2013-AdG 339431), the Medical Research Council (MRC) (G1100526/1) the Louis-Jeantet Foundation and the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO-VIDI 864.12.007). The Cambridge Stem Cell Institute receives core funding from the Wellcome Trust and Medical Research Council (MRC). A.S. is an MRC Professor. Deposited in PMC for immediate release

Performance of a fully‐automated system on a WHO malaria microscopy evaluation slide set

Background: Manual microscopy remains a widely-used tool for malaria diagnosis and clinical studies, but it has inconsistent quality in the field due to variability in training and field practices. Automated diagnostic systems based on machine learning hold promise to improve quality and reproducibility of field microscopy. The World Health Organization (WHO) has designed a 55-slide set (WHO 55) for their External Competence Assessment of Malaria Microscopists (ECAMM) programme, which can also serve as a valuable benchmark for automated systems. The performance of a fully-automated malaria diagnostic system, EasyScan GO, on a WHO 55 slide set was evaluated. Methods: The WHO 55 slide set is designed to evaluate microscopist competence in three areas of malaria diagnosis using Giemsa-stained blood films, focused on crucial field needs: malaria parasite detection, malaria parasite species identification (ID), and malaria parasite quantitation. The EasyScan GO is a fully-automated system that combines scanning of Giemsa-stained blood films with assessment algorithms to deliver malaria diagnoses. This system was tested on a WHO 55 slide set. Results: The EasyScan GO achieved 94.3 % detection accuracy, 82.9 % species ID accuracy, and 50 % quantitation accuracy, corresponding to WHO microscopy competence Levels 1, 2, and 1, respectively. This is, to our knowledge, the best performance of a fully-automated system on a WHO 55 set. Conclusions: EasyScan GO’s expert ratings in detection and quantitation on the WHO 55 slide set point towards its potential value in drug efficacy use-cases, as well as in some case management situations with less stringent species ID needs. Improved runtime may enable use in general case management settings

Tools to enable the study and translation of supramolecular amphiphiles

This tutorial review focuses on providing a summary of the key techniques used for the characterisation of supramolecular amphiphiles and their self-assembled aggregates; from the understanding of low-level molecular interactions, to materials analysis, use of data to support computer-aided molecular design and finally, the translation of this class of compounds for real world application, specifically within the clinical setting. We highlight the common methodologies used for the study of traditional amphiphiles and build to provide specific examples that enable the study of specialist supramolecular systems. This includes the use of nuclear magnetic resonance spectroscopy, mass spectrometry, x-ray scattering techniques (small- and wide-angle x-ray scattering and single crystal x-ray diffraction), critical aggregation (or micelle) concentration determination methodologies, machine learning, and various microscopy techniques. Furthermore, this review provides guidance for working with supramolecular amphiphiles in in vitro and in vivo settings, as well as the use of accessible software programs, to facilitate screening and selection of druggable molecules. Each section provides: a methodology overview – information that may be derived from the use of the methodology described; a case study – examples for the application of these methodologies; and a summary section – providing methodology specific benefits, limitations and future applications

A direct role for SNX9 in the biogenesis of filopodia.

Filopodia are finger-like actin-rich protrusions that extend from the cell surface and are important for cell-cell communication and pathogen internalization. The small size and transient nature of filopodia combined with shared usage of actin regulators within cells confounds attempts to identify filopodial proteins. Here, we used phage display phenotypic screening to isolate antibodies that alter the actin morphology of filopodia-like structures (FLS) in vitro. We found that all of the antibodies that cause shorter FLS interact with SNX9, an actin regulator that binds phosphoinositides during endocytosis and at invadopodia. In cells, we discover SNX9 at specialized filopodia in Xenopus development and that SNX9 is an endogenous component of filopodia that are hijacked by Chlamydia entry. We show the use of antibody technology to identify proteins used in filopodia-like structures, and a role for SNX9 in filopodia

Identification of Candidate Growth Promoting Genes in Ovarian Cancer through Integrated Copy Number and Expression Analysis

Ovarian cancer is a disease characterised by complex genomic rearrangements but the majority of the genes that are the target of these alterations remain unidentified. Cataloguing these target genes will provide useful insights into the disease etiology and may provide an opportunity to develop novel diagnostic and therapeutic interventions. High resolution genome wide copy number and matching expression data from 68 primary epithelial ovarian carcinomas of various histotypes was integrated to identify genes in regions of most frequent amplification with the strongest correlation with expression and copy number. Regions on chromosomes 3, 7, 8, and 20 were most frequently increased in copy number (>40% of samples). Within these regions, 703/1370 (51%) unique gene expression probesets were differentially expressed when samples with gain were compared to samples without gain. 30% of these differentially expressed probesets also showed a strong positive correlation (r≥0.6) between expression and copy number. We also identified 21 regions of high amplitude copy number gain, in which 32 known protein coding genes showed a strong positive correlation between expression and copy number. Overall, our data validates previously known ovarian cancer genes, such as ERBB2, and also identified novel potential drivers such as MYNN, PUF60 and TPX2