Simultaneous circulation of genotypes I and III of dengue virus 3 in Colombia

<p>Abstract</p> <p>Background</p> <p>Dengue is a major health problem in tropical and subtropical regions. In Colombia, dengue viruses (DENV) cause about 50,000 cases annually, 10% of which involve Dengue Haemorrhagic Fever/Dengue Shock Syndrome. The picture is similar in other surrounding countries in the Americas, with recent outbreaks of severe disease, mostly associated with DENV serotype 3, strains of the Indian genotype, introduced into the Americas in 1994.</p> <p>Results</p> <p>The analysis of the 3'end (224 bp) of the envelope gene from 32 DENV-3 strains recently recovered in Colombia confirms the circulation of the Indian genotype, and surprisingly the co-circulation of an Asian-Pacific genotype only recently described in the Americas.</p> <p>Conclusion</p> <p>These results have important implications for epidemiology and surveillance of DENV infection in Central and South America. Molecular surveillance of the DENV genotypes infecting humans could be a very valuable tool for controlling/mitigating the impact of the DENV infection.</p

Fossil Groups Origins III. The relation between optical and X-ray luminosities

This study is part of the FOssil Groups Origin (FOGO) project which aims at carrying out a systematic and multiwavelength study of a large sample of fossil systems. Here we focus on the relation between the optical luminosity (Lopt) and X-ray luminosity (Lx). Out of a sample of 28 candidate fossil systems, we consider a sample of 12 systems whose fossil classification has been confirmed by a companion study. They are compared with the complementary sample of 16 systems whose fossil nature is not confirmed and with a subsample of 102 galaxy systems from the RASS-SDSS galaxy cluster survey. Fossil and normal systems span the same redshift range 0<z<0.5 and have the same Lx distribution. For each fossil system, the Lx in the 0.1-2.4 keV band is computed using data from the ROSAT All Sky Survey. For each fossil and normal system we homogeneously compute Lopt in the r-band within the characteristic cluster radius, using data from the SDSS DR7. We sample the Lx-Lopt relation over two orders of magnitude in Lx. Our analysis shows that fossil systems are not statistically distinguishable from the normal systems both through the 2D KS test and the fit of the Lx-Lopt relation. The optical luminosity of the galaxy system does strongly correlate with the X-ray luminosity of the hot gas component, independently of whether the system is fossil or not. We conclude that our results are consistent with the classical "merging scenario" of the brightest galaxy formed via merger/cannibalism of other group galaxies, with conservation of the optical light. We find no evidence for a peculiar state of the hot intracluster medium.Comment: A&A, 12 pages, 4 figures, 3 tables, typos corr. and paper re-numbe

Towards a new classification of galaxies: principal component analysis of CALIFA circular velocity curves

We present a galaxy classification system for 238 (E1-Sdm) CALIFA (Calar Alto Legacy Integral Field Area) galaxies based on the shapes and amplitudes of their circular velocity curves (CVCs). We infer the CVCs from the de-projected surface brightness of the galaxies, after scaling by a constant mass-to-light ratio based on stellar dynamics - solving axisymmetric Jeans equations via fitting the second velocity moment $V_{\mathrm{rms}}=\sqrt{V^2+\sigma^2}$ of the stellar kinematics. We use principal component analysis (PCA) applied to the CVC shapes to find characteristic features and use a $k$-means classifier to separate circular curves into classes. This objective classification method identifies four different classes, which we name slow-rising (SR), flat (FL), round-peaked (RP) and sharp-peaked (SP) circular curves. SR are typical for low-mass, late-type (Sb-Sdm), young, faint, metal-poor and disc-dominated galaxies. SP are typical for high-mass, early-type (E1-E7), old, bright, metal-rich and bulge-dominated galaxies. FL and RP appear presented by galaxies with intermediate mass, age, luminosity, metallicity, bulge-to-disk ratio and morphologies (E4-S0a, Sa-Sbc). The discrepancy mass factor, $f_d=1-M_{*}/M_{dyn}$, have the largest value for SR and SP classes ($\sim$ 74 per cent and $\sim$ 71 per cent, respectively) in contrast to the FL and RP classes (with $\sim$ 59 per cent and $\sim$ 61 per cent, respectively). Circular curve classification presents an alternative to typical morphological classification and appears more tightly linked to galaxy evolution.Comment: Accepted for publication in MNRAS (Minor changes), 123 pages, 19 figures, 87 Tables (containing the basic properties of the 238 E1-Sdm galaxies; the five main Principal Component Eigenvectors; the five main Principal Components - PC_i; the Multi-Gaussian Expansion models - MGEs; the circular velocity curve models and their uncertainties

CALIFA : a diameter-selected sample for an integral field spectroscopy galaxy survey

JMA acknowledges support from the European Research Council Starting Grant (SEDmorph; P.I. V. Wild).We describe and discuss the selection procedure and statistical properties of the galaxy sample used by the Calar Alto Legacy Integral Field Area (CALIFA) survey, a public legacy survey of 600 galaxies using integral field spectroscopy. The CALIFA "mother sample" was selected from the Sloan Digital Sky Survey (SDSS) DR7 photometric catalogue to include all galaxies with an r-band isophotal major axis between 45 '' and 79 : 2 '' and with a redshift 0 : 005 M-r > -23 : 1 and over a stellar mass range between 10(9.7) and 10(11.4) M-circle dot. In particular, within these ranges, the diameter selection does not lead to any significant bias against - or in favour of - intrinsically large or small galaxies. Only below luminosities of M-r = -19 (or stellar masses <10(9.7) M-circle dot) is there a prevalence of galaxies with larger isophotal sizes, especially of nearly edge-on late-type galaxies, but such galaxies form <10% of the full sample. We estimate volume-corrected distribution functions in luminosities and sizes and show that these are statistically fully compatible with estimates from the full SDSS when accounting for large-scale structure. For full characterization of the sample, we also present a number of value-added quantities determined for the galaxies in the CALIFA sample. These include consistent multi-band photometry based on growth curve analyses; stellar masses; distances and quantities derived from these; morphological classifications; and an overview of available multi-wavelength photometric measurements. We also explore different ways of characterizing the environments of CALIFA galaxies, finding that the sample covers environmental conditions from the field to genuine clusters. We finally consider the expected incidence of active galactic nuclei among CALIFA galaxies given the existing pre-CALIFA data, finding that the final observed CALIFA sample will contain approximately 30 Sey2 galaxies.Peer reviewe

Disc galaxies are still settling: The discovery of the smallest nuclear discs and their young stellar bars

When galactic discs settle and become massive enough, they are able to form stellar bars. These non-axisymmetric structures induce shocks in the gas, causing it to flow to the centre where nuclear structures, such as nuclear discs and rings, are formed. Previous theoretical and observational studies have hinted at the co-evolution of bars and nuclear discs, suggesting that nuclear discs grow "inside-out", thereby proposing that smaller discs live in younger bars. Nevertheless, it remains unclear how the bar and the nuclear structures form and evolve with time. The smallest nuclear discs discovered to date tend to be larger than $\sim200~\rm{pc}$, even though some theoretical studies find that when nuclear discs form they can be much smaller. Using MUSE archival data, we report for the first time two extragalactic nuclear discs with radius sizes below $100~\rm{pc}$. Additionally, our estimations reveal the youngest bars found to date. We estimate that the bars in these galaxies formed $4.50^{+1.60}_{-1.10}\rm{(sys)}^{+1.00}_{-0.75}\rm{(stat)}$ and $0.7^{+2.60}\rm{(sys)}^{+0.05}_{-0.05}\rm{(stat)}~\rm{Gyr}$ ago, for NGC\,289 and NGC\,1566, respectively. This suggests that at least some disc galaxies in the Local Universe may still be dynamically settling. By adding these results to previous findings in the literature, we retrieve a stronger correlation between nuclear disc size and bar length and we derive a tentative exponential growth scenario for nuclear discs.Comment: Accepted in A&A (in press), 13 pages, 5 figures, 2 tabel

Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia

Background: Dengue Fever is one of the most important viral re-emergent diseases affecting about 50 million people around the world especially in tropical and sub-tropical countries. In Colombia, the virus was first detected in the earliest 70′s when the disease became a major public health concern. Since then, all four serotypes of the virus have been reported. Although most of the huge outbreaks reported in this country have involved dengue virus serotype 1 (DENV-1), there are not studies about its origin, genetic diversity and distribution. Results: We used 224 bp corresponding to the carboxyl terminus of envelope (E) gene from 74 Colombian isolates in order to reconstruct phylogenetic relationships and to estimate time divergences. Analyzed DENV-1 Colombian isolates belonged to the formerly defined genotype V. Only one virus isolate was clasified in the genotype I, likely representing a sole introduction that did not spread. The oldest strains were closely related to those detected for the first time in America in 1977 from the Caribbean and were detected for two years until their disappearance about six years later. Around 1987, a split up generated 2 lineages that have been evolving separately, although not major aminoacid changes in the analyzed region were found. Conclusion: DENV-1 has been circulating since 1978 in Colombia. Yet, the phylogenetic relationships between strains isolated along the covered period of time suggests that viral strains detected in some years, although belonging to the same genotype V, have different recent origins corresponding to multiple re-introduction events of viral strains that were circulating in neighbor countries. Viral strains used in the present study did not form a monophyletic group, which is evidence of a polyphyletic origin. We report the rapid spread patterns and high evolution rate of the different DENV-1 lineages

Phylogenetic reconstruction of dengue virus type 2 in Colombia

Background: Dengue fever is perhaps the most important viral re-emergent disease especially in tropical and subtropical countries, affecting about 50 million people around the world yearly. In Colombia, dengue virus was first detected in 1971 and still remains as a major public health issue. Although four viral serotypes have been recurrently identified, dengue virus type 2 (DENV-2) has been involved in the most important outbreaks during the last 20 years, including 2010 when the fatality rate highly increased. As there are no major studies reviewing virus origin and genotype distribution in this country, the present study attempts to reconstruct the phylogenetic history of DENV-2 using a sequence analysis from a 224 bp PCR-amplified product corresponding to the carboxyl terminus of the envelope (E) gene from 48 Colombian isolates. Results: As expected, the oldest isolates belonged to the American genotype (subtype V), but the strains collected since 1990 represent the American/Asian genotype (subtype IIIb) as previously reported in different American countries. Interestingly, the introduction of this genotype coincides with the first report of dengue hemorrhagic fever in Colombia at the end of 1989 and the increase of cases during the next years. Conclusion: After replacement of the American genotype, several lineages of American/Asian subtype have rapidly spread all over the country evolving in new clades. Nevertheless, the direct association of these new variants in the raise of lethality rate observed during the last outbreak has to be demonstrated

The intrinsic shape and dynamical structure of the bulges of lenticular galaxies

This work was supported by Padua University through grants 60A02-5052/11, 60A02-4807/12, 60A02-5857/13, and CPDA133894. J.M.A. acknowledges support from the European Research Council Starting Grant (SEDmorph; P.I. V. Wild). L.M. received financial support from Padua University grant CPS0204.Several scenarios have been proposed to account for the formation and evolution of galactic bulges and to explain the variety of their observed properties. Both the intrinsic shape and dynamical structure of bulges depend on the mechanisms and timescales of their formation. We are deriving the intrinsic axial ratios of the bulges of a sample of unbarred lenticular galaxies to look for a possible relationship with their known orbital structure. Preliminary results show that the orbits of stars of the triaxial bulge of NGC 4476 are more anisotropic than those in the axisymmetric bulge of NGC 4249 hinting at a different formation process.Publisher PDFPeer reviewe

Large scale use of SARS-CoV-2 antigen-based detection tests: a three-month experience in Guatemala, June-August 2020

Objectives. To measure protocol adherence and antigen-based detection tests (AgDT) negative predictive value after 3 months of massive use as a diagnostic tool for COVID-19 in Guatemala. Methods. The study period included nasopharyngeal swabs taken between March 12 and August 31, 2020, which results were entered in the national COVID-19 information system. Proportional increase in testing between one month before and one month after the introduction of AgDT (May 9–June 8 vs. June 9–July 8) was measured. Results. After AgDT introduction, there was a 139% increase in SARS-CoV-2 testing. Between June 9 and August 31, 7.8% of 110 657 AgDT-negative patients had follow-up RT-PCR testing. Of them, 30% were RT-PCR positive. Conclusions. While introducing AgDT improved access to diagnostics, ensuring the availability of timely RT-PCR capacities to confirm diagnosis is also key

Music training and auditory processing skills in children : a systematic review

Objetivo: Verificar a contribuição do treinamento musical nas habilidades do processamento auditivo em crianças. Estratégia de pesquisa: Realizou-se uma busca no mês de agosto de 2018, usando os descritores Music, Child, Childhood, Children, Evoked Potentials, Auditory, Auditory Perception, Auditory Processing, utilizando o operador AND. Critérios de seleção: Como questão norteadora, adotou-se a seguinte pergunta: “o que existe na literatura científica sobre a contribuição do treinamento musical nas habilidades de processamento auditivo em crianças?” Após, foram selecionados somente ensaios clínicos controlados na população infantil, estudos publicados em inglês, português e espanhol. Resultados: A estratégia de busca resultou na seleção de dez artigos. Os estudos evidenciaram diversas habilidades testadas e diferentes formas de avaliação. Conclusão: Com base nos achados, pode-se concluir que o treinamento musical melhora e aprimora as habilidades de processamento auditivo, de forma que quanto maior o tempo de treinamento, mais essas habilidades são reforçadas. Dessa forma, o treinamento musical mostra-se um método eficaz e com potencialidade para ser utilizado em crianças, tanto no período de desenvolvimento da comunicação oral e escrita, para auxiliar a aquisição das habilidades auditivas, como após a aquisição afim de aprimorá-las.Purpose: To check the contribution of music training to auditory processing skills in children. Research strategy: A search was performed in October 2018, using the descriptors Music, Child, Childhood, Children, Evoked Potentials, Auditory, Auditory Perception, Auditory Processing, using the AND operator. Selection criteria: The main research question was: “What has been reported in the scientific literature about the contribution of musical training to auditory processing skills in children?” We selected only controlled clinical trials with the child population, studies published in English, Portuguese and Spanish. Results: The search strategy resulted in the selection of ten articles. The studies showed several skills tested and different forms of evaluation. Conclusion: Based on the findings, it can be concluded that music training improves auditory processing skills, so the longer the training time, the more these skills are reinforced. Thus, music training is an effective method that can be potentially used in children, both in the development of oral and written communication - to aid in the acquisition of auditory skills - and after acquisition, in order to improve such skills