Correction of moisture effects on near infrared calibration for the analysis of phenol content in eucalyptus wood extracts

Les méthodes basées sur la spectroscopie proche infrarouge pour estimer les propriétés du bois sont sensibles aux variations des paramètres physiques (température, granulométrie. . . ). Le bois étant un matériau hygroscopique sensible, l'influence de l'humidité sur l'absorbance et l'étalonnage proche infrarouge a été étudiée afin de mieux considérer les possibilités d'applications dans des conditions réelles.Un étalonnage de référence de la quantité de polyphénols présents dans les extraits a été établi à partir de spectres d'une collection de bois d'hybrides d'Eucalyptus urophylla × E. grandis à humidité constante fixée. D'autres spectres ont été obtenus sur des échantillons de même provenance mais à 8 teneurs en eau couvrant une large plage de variation. L'influence de l'humidité sur l'absorbance proche infrarouge puis sur l'estimation par le modèle de référence a été analysée. / Methods based on near infrared spectroscopy used to assess wood properties are susceptible to variations in physical parameters (temperature, grain size, etc). As wood is a hygroscopically sensitive material, we studied the effects of moisure on near infrared absorbance and calibration to accurately determine the application potential of this technique under routine. A collection of Eucalyptus urophylla x E. grandis hybrid wood pieces were analysed to obtain reference calibration of polyphenol contents in wood extracts via NIR spectra acquired under constant moisture conditions.Others specimens from the same source were assessed to obtain spectra for eight moisture contents spanning a broad variation range. The effects of moisture on absorption and on estimates based on a reference model were analysed. An increase in moisture content prompteda rise in near infrared absorption over the entire spectrum and the water O-H absorption bands. The polyphenol content estimates obtained by assessing specimens against the reference calibration at variable moisture contents revealed prediction bias. Five correction methods were then tested on enhance the robustness relative to moisture. In-depth calibration and external parameter orthogonalization were found to be the most efficient methods for offsetting this factor

Oakscan: procédé de mesure rapide et non destructif des polyphénols du bois de chêne de tonnellerie

Les polyphénols extractibles contenus dans le bois de chêne des barriques de tonnellerie sont des composés très importants car ils ont une influence sur la couleur et sur les propriétés organoleptiques des vins qu'ils enrichissent. Les analyses chimiques de laboratoire qui permettent de les mesurer sont réalisées sur des extractions, nécessitent un délai de réalisation très long et ne permettent d'analyser que des lots de matière par échantillonnage. Le procédé de mesure rapide et non destructif Oakscan® a été développé par la tonnellerie Radoux. Il se fonde sur la spectrométrie proche infrarouge et permet de mesurer la teneur en polyphénols en quelques secondes, directement sur le bois massif. Le contenu polyphénolique de chaque douelle est alors mesuré, permettant une meilleure maitrise de la matière première entrant dans la composition des fûts

Contents : Chiba Medical Journal Volume 92 Number 1 , Fabruary 2016

International audienc

Identification of Antigenic Proteins from Lichtheimia corymbifera for Farmer's Lung Disease Diagnosis.

The use of recombinant antigens has been shown to improve both the sensitivity and the standardization of the serological diagnosis of Farmer's lung disease (FLD). The aim of this study was to complete the panel of recombinant antigens available for FLD serodiagnosis with antigens of Lichtheimia corymbifera, known to be involved in FLD. L. corymbifera proteins were thus separated by 2D electrophoresis and subjected to western blotting with sera from 7 patients with FLD and 9 healthy exposed controls (HEC). FLD-associated immunoreactive proteins were identified by mass spectrometry based on a protein database specifically created for this study and subsequently produced as recombinant antigens. The ability of recombinant antigens to discriminate patients with FLD from controls was assessed by ELISA performed with sera from FLD patients (n = 41) and controls (n = 43) recruited from five university hospital pneumology departments of France and Switzerland. Forty-one FLD-associated immunoreactive proteins from L. corymbifera were identified. Six of them were produced as recombinant antigens. With a sensitivity and specificity of 81.4 and 77.3% respectively, dihydrolipoyl dehydrogenase was the most effective antigen for discriminating FLD patients from HEC. ELISA performed with the putative proteasome subunit alpha type as an antigen was especially specific (88.6%) and could thus be used for FLD confirmation. The production of recombinant antigens from L. corymbifera represents an additional step towards the development of a standardized ELISA kit for FLD diagnosis

Nucleation of Al3Zr and Al3Sc in aluminum alloys: from kinetic Monte Carlo simulations to classical theory

Zr and Sc precipitate in aluminum alloys to form the compounds Al3Zr and Al3Sc which for low supersaturations of the solid solution have the L12 structure. The aim of the present study is to model at an atomic scale this kinetics of precipitation and to build a mesoscopic model based on classical nucleation theory so as to extend the field of supersaturations and annealing times that can be simulated. We use some ab-initio calculations and experimental data to fit an Ising model describing thermodynamics of the Al-Zr and Al-Sc systems. Kinetic behavior is described by means of an atom-vacancy exchange mechanism. This allows us to simulate with a kinetic Monte Carlo algorithm kinetics of precipitation of Al3Zr and Al3Sc. These kinetics are then used to test the classical nucleation theory. In this purpose, we deduce from our atomic model an isotropic interface free energy which is consistent with the one deduced from experimental kinetics and a nucleation free energy. We test di erent mean-field approximations (Bragg-Williams approximation as well as Cluster Variation Method) for these parameters. The classical nucleation theory is coherent with the kinetic Monte Carlo simulations only when CVM is used: it manages to reproduce the cluster size distribution in the metastable solid solution and its evolution as well as the steady-state nucleation rate. We also find that the capillary approximation used in the classical nucleation theory works surprisingly well when compared to a direct calculation of the free energy of formation for small L12 clusters.Comment: submitted to Physical Review B (2004

Single-neutron transfer from 11Be gs via the (p,d) reaction with a radioactive beam

The 11Be(p,d)10Be reaction has been performed in inverse kinematics with a radioactive 11Be beam of E/A = 35.3 MeV. Angular distributions for the 0+ ground state, the 2+, 3.37 MeV state and the multiplet of states around 6 MeV in 10Be were measured at angles up to 16 deg CM by detecting the 10Be in a dispersion-matched spectrometer and the coincident deuterons in a silicon array. Distorted wave and coupled-channels calculations have been performed to investigate the amount of 2+ core excitation in 11Be gs. The use of "realistic" 11Be wave functions is emphasised and bound state form factors have been obtained by solving the particle-vibration coupling equations. This calculation gives a dominant 2s component in the 11Be gs wave function with a 16% [2+ x 1d] core excitation admixture. Cross sections calculated with these form factors are in good agreement with the present data. The Separation Energy prescription for the bound state wave function also gives satisfactory fits to the data, but leads to a significantly larger [2 x 1d] component in 11Be gs.Comment: 39 pages, 12 figures. Accepted for publication in Nuclear Physics A. Added minor corrections made in proof to pages 26 and 3

Inverse kinematics proton scattering from the exotic nucleus 22^{22}O

NESTER PTHInternational audienceWe have measured for the first time elastic and inelastic proton scattering from the neutron rich nucleus 22O at 46.6 AMeV using the MUST array

Virtual coupling potential for elastic scattering of 10,11^{10,11}Be on proton and carbon targets

International audienceThe 10;11Be(p,p) and (12C, 12C) reactions were analyzed to determine the in uence of the weak binding energies of exotic nuclei on their interaction potential. The elastic cross sections were measured at GANIL in inverse kinematics using radioactive 10;11Be beams produced at energies of 39:1 A and 38:4A MeV. The elastic proton scattering data were analyzed within the framework of the microscopic Jeukenne-Lejeune-Mahaux (JLM) nucleon-nucleus potential. The angular distributions are found to be best reproduced by reducing the real part of the microscopic optical potential, as a consequence of the coupling to the continuum. These effects modify deeply the elastic potential. Including the Virtual Coupling Potential (VCP), we show the ability of the general optical potentials to reproduce the data for scattering of unstable nuclei, using realistic densities. Finally, the concepts needed to develop a more general and microscopic approach of the VCP are discussed

The N=14 shell closure in 22^{22}O viewed through a neutron sensitive probe

NESTER PTHInternational audienceTo investigate the behavior of the N=14 neutron gap far from stability with a neutron-sensitive probe, proton elastic and 2+1 inelastic scattering angular distributions for the neutron-rich nucleus 22O were measured with a secondary beam intensity of only 1200 particles per second using the MUST silicon strip detector array at the GANIL facility. A phenomenological analysis yields a deformation parameter bp;p' = 0.26 +- 0.04 for the 2+1 state, much lower than in 20O, showing a surprisingly weak neutron contribution to this state. A fully microscopic analysis was performed using optical potentials obtained from matter and transition densities generated by continuum Skyrme-HFB and QRPA calculations, respectively. When the present results and those from a 22O + 197Au scattering experiment are combined, the ratio of neutron to proton contributions to the 2+1 state is found close to the N/Z ratio, demonstrating a strong N=14 shell closure in the vicinity of the neutron drip-line

Coupling effects in the elastic scattering of 6^{6}He on 12^{12}C

To study the effect of the weak binding energy on the interaction potential between a light exotic nucleus and a target, elastic scattering of 6He at 38.3 MeV/nucleon on a 12C target was measured at Grand Accélérateur National d'Ions Lourds (GANIL). The 6He beam was produced by fragmentation. The detection of the scattered particles was performed by the GANIL spectrometer. The energy resolution was good enough to separate elastic from inelastic scattering contributions. The measured elastic data have been analyzed within the optical model, with the real part of the optical potential calculated in the double-folding model using a realistic density-dependent nucleon-nucleon interaction and the imaginary part taken in the conventional Woods-Saxon (WS) form. A failure of the "bare" real folded potential to reproduce the measured angular distribution over the whole angular range suggests quite a strong coupling of the higher-order breakup channels to the elastic channel. To estimate the strength of the breakup effects, a complex surface potential with a repulsive real part (designed to simulate the polarization effects caused by the projectile breakup) was added to the real folded and imaginary WS potentials. A realistic estimate of the polarization potential caused by the breakup of the weakly bound 6He was made based on a parallel study of 6He+12C and 6Li+12C optical potentials at about the same energies