Jan Verschueren's Description of Yéi-Nan Culture

Joannis Cornelis Verschueren (1905-1970) worked as a Roman Catholic missionary in southern Irian from 1931 until 1970. In the 1950s he wrote a number of papers on the

Generalised Kanzaki force field of extended defects in crystals, with applications to the modelling of edge dislocations

The Kanzaki forces and their associated multipolar moments are standard ways of representing point defects in an atomistically informed way in the continuum. In this article, the Kanzaki force approach is extended to other crystalline defects. The article shows how the resulting Kanzaki force fields are to be computed for any general extended defect by first computing the relaxed defect's structure and then defining an affine mapping between the said defect structure and the original perfect lattice. This methodology can be employed to compute the Kanzaki force field of any mass-conserving defect, including dislocations, grain and twin boundaries, or cracks. Particular focus is then placed on straight edge dislocation in face-centered cubic (fcc) and body-centered cubic (bcc) pure metals, which are studied along different crystallographic directions. The particular characteristics of these force fields are discussed, drawing a distinction between the slip Kanzaki force field associated with the Volterra disregistry that characterizes the dislocation, and the core Kanzaki force field associated with the specific topology of the dislocation's core. The resulting force fields can be employed to create elastic models of the dislocation that, unlike other regularization procedures, offer a geometrically true representation of the core and the elastic fields in its environs, capturing all three-dimensional effects associated with the core

Laboratory study of the impact of repetitive electrical and mechanical stimulation on brown shrimp Crangon crangon

Pulse trawling is currently the best available alternative to beam trawling in the brown shrimp Crangon crangon and Sole Solea solea (also known as Solea vulgaris) fisheries. To evaluate the effect of repetitive exposure to electrical fields, brown shrimp were exposed to the commercial electrodes and pulse settings used to catch brown shrimp (shrimp startle pulse) or Sole (Sole cramp pulse) 20 times in 4 d and monitored for up to 14 d after the first exposure. Survival, egg loss, molting, and the degree of intranuclear bacilliform virus (IBV) infection were evaluated and compared with those in stressed but not electrically exposed (procedural control) and nonstressed, nonexposed (control) brown shrimp as well as brown shrimp exposed to mechanical stimuli. The lowest survival at 14 d (57.3%) occurred in the Sole cramp pulse treatment, and this was significantly lower than in the group with the highest survival, the procedural control (70.3%). No effect of electrical stimulation on the severity of IBV infection was found. The lowest percentage of molts occurred in the repetitive mechanical stimulation treatment (14.0%), and this was significantly lower than in the group with the highest percentage of molts, the procedural control (21.7%). Additionally, the mechanically stimulated brown shrimp that died during the experiment had a significantly larger size than the surviving individuals. Finally, no effect of the shrimp startle pulse was found. Therefore, it can be concluded that repetitive exposure to a cramp stimulus and mechanical stimulation may have negative effects on the growth and/or survival of brown shrimp. However, there is no evidence that electrical stimulation during electrotrawls would have a larger negative impact on brown shrimp stocks than mechanical stimulation during conventional beam trawling

Repositioning the Catalytic Triad Aspartic Acid of Haloalkane Dehalogenase: Effects on Stability, Kinetics, and Structure

Haloalkane dehalogenase (DhlA) catalyzes the hydrolysis of haloalkanes via an alkyl-enzyme intermediate. The covalent intermediate, which is formed by nucleophilic substitution with Asp124, is hydrolyzed by a water molecule that is activated by His289. The role of Asp260, which is the third member of the catalytic triad, was studied by site-directed mutagenesis. Mutation of Asp260 to asparagine resulted in a catalytically inactive D260N mutant, which demonstrates that the triad acid Asp260 is essential for dehalogenase activity. Furthermore, Asp260 has an important structural role, since the D260N enzyme accumulated mainly in inclusion bodies during expression, and neither substrate nor product could bind in the active-site cavity. Activity for brominated substrates was restored to D260N by replacing Asn148 with an aspartic or glutamic acid. Both double mutants D260N+N148D and D260N+N148E had a 10-fold reduced kcat and 40-fold higher Km values for 1,2-dibromoethane compared to the wild-type enzyme. Pre-steady-state kinetic analysis of the D260N+N148E double mutant showed that the decrease in kcat was mainly caused by a 220-fold reduction of the rate of carbon-bromine bond cleavage and a 10-fold decrease in the rate of hydrolysis of the alkyl-enzyme intermediate. On the other hand, bromide was released 12-fold faster and via a different pathway than in the wild-type enzyme. Molecular modeling of the mutant showed that Glu148 indeed could take over the interaction with His289 and that there was a change in charge distribution in the tunnel region that connects the active site with the solvent. On the basis of primary structure similarity between DhlA and other α/β-hydrolase fold dehalogenases, we propose that a conserved acidic residue at the equivalent position of Asn148 in DhlA is the third catalytic triad residue in the latter enzymes.

Radial velocities of early-type stars in the Perseus OB2 association

We present radial velocities for 29 B- and A-type stars in the field of the nearby association Perseus OB2. The velocities are derived from spectra obtained with AURELIE, via cross correlation with radial velocity standards matched as closely as possible in spectral type. The resulting accuracy is ~2 - 3 km s$^{-1}$. We use these measurements, together with published values for a few other early-type stars, to study membership of the association. The mean radial velocity (and measured velocity dispersion) of Per OB2 is 23.5 \pm 3.9 km s$^{-1}$, and lies ~15 km s$^{-1}$ away from the mean velocity of the local disk field stars. We identify a number of interlopers in the list of possible late-B- and A-type members which was based on Hipparcos parallaxes and proper motions, and discuss the colour-magnitude diagram of the association.Comment: 20 pages, 9 figures, accepted for publication in A&A, minor revision

Variations in Stellar Clustering with Environment: Dispersed Star Formation and the Origin of Faint Fuzzies

The observed increase in star formation efficiency with average cloud density, from several percent in whole giant molecular clouds to ~30 or more in cluster-forming cores, can be understood as the result of hierarchical cloud structure if there is a characteristic density as which individual stars become well defined. Also in this case, the efficiency of star formation increases with the dispersion of the density probability distribution function (pdf). Models with log-normal pdf's illustrate these effects. The difference between star formation in bound clusters and star formation in loose groupings is attributed to a difference in cloud pressure, with higher pressures forming more tightly bound clusters. This correlation accounts for the observed increase in clustering fraction with star formation rate and with the observation of Scaled OB Associations in low pressure environments. ``Faint fuzzie'' star clusters, which are bound but have low densities, can form in regions with high Mach numbers and low background tidal forces. The proposal by Burkert, Brodie & Larsen (2005) that faint fuzzies form at large radii in galactic collisional rings, satisfies these constraints.Comment: 14 pages, 2 figures, ApJ, 672, January 10th 200

The shape of the initial cluster mass function: what it tells us about the local star formation efficiency

We explore how the expulsion of gas from star-cluster forming cloud-cores due to supernova explosions affects the shape of the initial cluster mass function, that is, the mass function of star clusters when effects of gas expulsion are over. We demonstrate that if the radii of cluster-forming gas cores are roughly constant over the core mass range, as supported by observations, then more massive cores undergo slower gas expulsion. Therefore, for a given star formation efficiency, more massive cores retain a larger fraction of stars after gas expulsion. The initial cluster mass function may thus differ from the core mass function substantially, with the final shape depending on the star formation efficiency. A mass-independent star formation efficiency of about 20 per cent turns a power-law core mass function into a bell-shaped initial cluster mass function, while mass-independent efficiencies of order 40 per cent preserve the shape of the core mass function.Comment: accepted in Ap

Two-stage muscle activity responses in decisions about leg movement adjustments during trip recovery

Item does not contain fulltextStudies on neural decision making mostly investigated fast corrective adjustments of arm movements. However, fast leg movement corrections deserve attention as well, since they are often required to avoid falling after balance perturbations. The present study aimed at elucidating the mechanisms behind fast corrections of tripping responses by analyzing the concomitant leg muscle activity changes. This was investigated in seven young adults who were tripped in between normal walking trials and took a recovery step by elevating the tripped leg over the obstacle. In some trials, a forbidden landing zone (FZ) was presented behind the obstacle, at the subjects' preferred foot landing position, forcing a step correction. Muscle activity of the tripped leg gastrocnemius medialis (iGM), tibialis anterior (iTA), rectus femoris (iRF), and biceps femoris (iBF) muscles was compared between normal trips presented before any FZ appearance, trips with a FZ, and normal trips presented in between trips with a FZ ("catch" trials). When faced with a real or expected (catch trials) FZ, subjects shortened their recovery steps. The underlying changes in muscle activity consisted of two stages. The first stage involved reduced iGM activity, occurring at a latency shorter than voluntary reaction, followed by reduced iTA and increased iBF and iGM activities occurring at longer latencies. The fast response was not related to step shortening, but longer latency responses clearly were functional. We suggest that the initial response possibly acts as a "pause," allowing the nervous system to integrate the necessary information and prepare the subsequent, functional movement adjustment

The injection of a screw dislocation into a crystal: Atomistics vs. continuum elastodynamics

EPSR

PIKES Analysis Reveals Response to Degraders and Key Regulatory Mechanisms of the CRL4 Network

Co-opting Cullin4 RING ubiquitin ligases (CRL4s) to inducibly degrade pathogenic proteins is emerging as a promising therapeutic strategy. Despite intense efforts to rationally design degrader molecules that co-opt CRL4s, much about the organization and regulation of these ligases remains elusive. Here, we establish protein interaction kinetics and estimation of stoichiometries (PIKES) analysis, a systematic proteomic profiling platform that integrates cellular engineering, affinity purification, chemical stabilization, and quantitative mass spectrometry to investigate the dynamics of interchangeable multiprotein complexes. Using PIKES, we show that ligase assemblies of Cullin4 with individual substrate receptors differ in abundance by up to 200-fold and that Cand1/2 act as substrate receptor exchange factors. Furthermore, degrader molecules can induce the assembly of their cognate CRL4, and higher expression of the associated substrate receptor enhances degrader potency. Beyond the CRL4 network, we show how PIKES can reveal systems level biochemistry for cellular protein networks important to drug development