First report of Pyrrhocalles antiqua orientis (Skinner) (Lepidoptera: Hesperidae) infesting the Bermuda turfgrass in Cuba

 Dear editor:Butterflies have proven to be excellent indicators of the disturbance and biodiversity of habitats worldwide. Skipper (Hesperiidae) remains one of the most diverse and least known butterfly group. Pyrrhocalles Mabille (1904) (Lepidoptera: Hesperiidae) is a small genus endemic to the Greater Antilles, and Pyrrhocalles antiqua Herrich-Schäffer is found across the Caribbean. Three subspecies have been described, and Pyrrhocalles antiqua orientis Skinner is an endemic subspecies of Cuba. However, the knowledge about its distribution, population size, and caterpillar host plants is limited. In 2007, P. antiqua orientis caterpillars have been reported on Roystonea regia (H.B.K.), family Arecaceae, in Camaguey province, Cuba. Recently, samples collected from the Varadero golf field, Matanzas province, showed P. antiqua orientis caterpillars to be present causing defoliations on Bermuda turfgrass (Cynodon dactylon (L.) Pers), family Poaceae. Several pupae and twenty-six adults were also captured on greens. To our knowledge, this is the first evidence of P. antiqua orientis infesting Bermuda turfgrass in Cuba. The occurrence of P. antiqua orientis in coastal ecosystems, including the north littoral of the island, has been described. Caterpillars of the subfamily Hesperiinae have been reported feeding only on gramineas (Poaceae). These results suggest the need for carrying out observations of P. antiqua orientis populations and including this insect in the management strategies of the Varadero golf field.Sincerely

Development and kinematic verification of a finite element model for the lumbar spine: Application to disc degeneration

The knowledge of the lumbar spine biomechanics is essential for clinical applications. Due to the difficulties to experiment on living people and the irregular results published, simulation based on finite elements (FE) has been developed, making it possible to adequately reproduce the biomechanics of the lumbar spine. A 3D FE model of the complete lumbar spine (vertebrae, discs, and ligaments) has been developed. To verify the model, radiological images (X-rays) were taken over a group of 25 healthy, male individuals with average age of 27.4 and average weight of 78.6 kg with the corresponding informed consent. A maximum angle of 34.40° is achieved in flexion and of 35.58° in extension with a flexion-extension angle of 69.98°. The radiological measurements were 33.94 ± 4.91°, 38.73 ± 4.29°, and 72.67°, respectively. In lateral bending, the maximum angles were 19.33° and 23.40 ± 2.39, respectively. In rotation a maximum angle of 9.96° was obtained. The model incorporates a precise geometrical characterization of several elements (vertebrae, discs, and ligaments), respecting anatomical features and being capable of reproducing a wide range of physiological movements. Application to disc degeneration (L5-S1) allows predicting the affection in the mobility of the different lumbar segments, by means of parametric studies for different ranges of degeneration

Traumatic brain injury: Failure of the intravenous route for the administration of bone marrow stromal stem cells as treatment of chronic neurological sequels

Objetivo: Estudiar el posible efecto terapéutico de la administración intravenosa de células madre estromales (CME) obtenidas de médula ósea para tratar las secuelas neurológicas en fase crónica tras una lesión cerebral traumática. Material y método: Se realizó un modelo de lesión cerebral traumática en ratas Wistar adultas y se estudió el déficit neurológico inducido en el curso de los dos meses siguientes, por medio del test mNSS y el test Smart. Tras ese tiempo, en fase de secuelas crónicamente establecidas, se administraron intravenosamente 15 x 106 CME (n:10) o suero fisiológico (n:10). En los dos meses siguientes se estudió la posible modificación de las secuelas neurológicas. Resultados: Cuando se compararon los resultados de la valoración funcional entre ambos grupos experimentales, no se observaron diferencias estadísticamente significativas. Conclusión: Nuestros resultados sugieren que el trasplante de CME por vía intravenosa, en una fase de secuelas crónicamente establecidas tras una lesión traumática cerebral grave, no tiene efecto terapéuticoObjective: We studied the possible therapeutic effect of intravenous administration (noninvasive method) of BMSCs to treat neurological sequels in a chronic stage after TBI. Material and method: A model of TBI in adult Wistar rats was performed and we studied the neurological deficit induced in the course of two months, through the mNSS and Smart tests. After this time, with established sequels, 15 x 106 BMSCs (n = 10) or saline (n = 10) were administered intravenously. Changes in the neurological deficits were studied in two months. Results: Comparison of functional changes between both experimental groups showed no statistically significant differences. Conclusions: Our results suggest that transplantation of BMSCs intravenously, at a stage of established sequels after severe TBI, has no therapeutic effectEsta investigación ha sido financiada por FUNDACIÓN MAPFR

Tsunami Resonance in Palma Bay and Harbor, Majorca Island, as induced by the 2003 Western Mediterranean Earthquake

ABSTRACT: he tsunami induced by the May 21, 2003, Algerian Boumerde`s-Zemmouri earthquake (moment magnitude Mw=6.9) propagated across the western Mediterranean Basin, thereby causing material damages in some harbors and coastal areas. This was the case in the Balearic Islands and particularly the Palma harbor. Attempts to simulate the 2003 tsunami event found discrepancies between tsunami arrival times and wave amplitude when comparing tide gauge records with results from numerical models. To date, all published model results of the amplitude of the tsunami are underestimations, attributed to numerical limitations due to the lack of high-resolution bathymetry and poor definition of harbor geometry. Other views suggest the nappropriateness of the available seismic source parameterizations and the possible occurrence of submarine landsliding triggered by the earthquake that has not been included in the numerical simulations. In this article we present the results of a numerical study aimed at better understanding the response of the interacting Palma bay and harbor under the impact of the 2003 western Mediterranean tsunami. The transference of the tsunami energy from the generation area to the continental shelf, the bay, and the harbor has been studied and compared with the natural oscillation modes of the bay and harbor water bodies. Our work includes a sensitivity analysis of the source parameterization and the bathymetry grid size for the bay and harbor as a way to explain the discrepancies between simulations and observations. The Palma harbor tide gauge shows that energy from the tsunami concentrated mainly in periods that fitted to the natural modes of oscillations of the bay. Therefore, the significant wave amplification observed inside the harbor, mainly in its northern basin, was generated by a resonance effect induced by Palma bay. The improvement of the bathymetry grid resolution in the bay and harbor domains and the inclusion in the simulations of the exact harbor geometry and internal configuration result in a slight wave-high increment that is much below the wave height recorded in the tide gauge. Our results strongly point to a necessary revision of the tsunami seismic source parameters

Perfil Clínico del tratamiento de infección del tracto urinario con antibióticos de amplio espectro en un hospital 2012

Objetivo. Determinar el perfil clínico del tratamiento de la infección del tracto urinario (ITU) con antibióticos de amplio espectro en el Hospital Central de la Fuerza Aérea del Perú, entre los meses de Agosto y Noviembre del 2012. Materiales y métodos Estudio observacional, descriptivo y transversal realizado en el Hospital Central de la Fuerza Aérea de Lima-Perú, entre los meses de Agosto y Noviembre del 2012. Se tomo 71 historias clínicas de pacientes con diagnóstico de ITU que recibieron antibióticos de amplio espectro. Se analizó las variables con frecuencias (cualitativas), medidas de tendencia central y dispersión (cuantitativas). Los datos fueron procesados en el paquete estadístico STATA 11.0. Resultados. La edad promedio fue 67.08 ± 19.21 años, el 61,43% (n=43) fueron varones y el 38,57% (n=27) mujeres. El servicio con mayor prescripción fue el Servicio de Emergencia 44,29% (n=31). La comorbilidad más frecuente fue Diabetes Mellitus (DM) 24,29% (n =17). La clínica más frecuente fue disuria 35,71% (n=25). El hallazgo de laboratorio más frecuente fue leucocitosis 65,71% (n=46). Y se prescribió con mayor frecuencia ceftriaxona 34,29% (n=24). Conclusión. El perfil clínico del tratamiento de ITU con antibióticos de amplio espectro es diverso, asociándose con mayor frecuencia a la presencia de antecedente de DM y disuria

El análisis proteómico mediante 2D-DIGE y MS/MS permite discriminar en un mismo experimento entre ratones silvestres (WT) o deficientes CD38 (CD38ko) y entre ratones afectados o no por la artritis inducida por colágeno

Comunicaciones a congreso

Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners

This article belongs to the Special Issue TGF-beta/BMP Signaling PathwayEndoglin is a 180-kDa glycoprotein receptor primarily expressed by the vascular endothelium and involved in cardiovascular disease and cancer. Heterozygous mutations in the endoglin gene (ENG) cause hereditary hemorrhagic telangiectasia type 1, a vascular disease that presents with nasal and gastrointestinal bleeding, skin and mucosa telangiectases, and arteriovenous malformations in internal organs. A circulating form of endoglin (alias soluble endoglin, sEng), proteolytically released from the membrane-bound protein, has been observed in several inflammation-related pathological conditions and appears to contribute to endothelial dysfunction and cancer development through unknown mechanisms. Membrane-bound endoglin is an auxiliary component of the TGF-ß receptor complex and the extracellular region of endoglin has been shown to interact with types I and II TGF-ß receptors, as well as with BMP9 and BMP10 ligands, both members of the TGF-ß family. To search for novel protein interactors, we screened a microarray containing over 9000 unique human proteins using recombinant sEng as bait. We find that sEng binds with high affinity, at least, to 22 new proteins. Among these, we validated the interaction of endoglin with galectin-3, a secreted member of the lectin family with capacity to bind membrane glycoproteins, and with tripartite motif-containing protein 21 (TRIM21), an E3 ubiquitin-protein ligase. Using human endothelial cells and Chinese hamster ovary cells, we showed that endoglin co-immunoprecipitates and co-localizes with galectin-3 or TRIM21. These results open new research avenues on endoglin function and regulation.This work was supported by grants from Ministerio de Ciencia, Innovación y Universidades of Spain (SAF2013-43421-R to CB and SAF2017-84183-R to MQ), Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER; ISCIII-CB06/07/0038 to CB and contract CNV-234-PRF-360 to LR-L) and Consejo Superior de Investigaciones Científicas (CSIC; 201920E022 to CB). JC-V was supported by a postdoctoral contract co-funded by Consejo Superior de Investigaciones Científicas, Ministerio de Ciencia, Innovación y Universidades and the European Social Fund (ESF). CIBERER and CIBERNED are initiatives of the Instituto de Salud Carlos III (ISCIII) of Spain supported by European Regional Development (FEDER) funds

Intraarticular Administration Effect of Hydrogen Sulfide on an In Vivo Rat Model of Osteoarthritis

[Abstract] Osteoarthritis (OA) is the most common articular chronic disease. However, its current treatment is limited and mostly symptomatic. Hydrogen sulfide (H2S) is an endogenous gas with recognized physiological activities. The purpose here was to evaluate the effects of the intraarticular administration of a slow-releasing H2S compound (GYY-4137) on an OA experimental model. OA was induced in Wistar rats by the transection of medial collateral ligament and the removal of the medial meniscus of the left joint. The animals were randomized into three groups: non-treated and intraarticularly injected with saline or GYY-4137. Joint destabilization induced articular thickening (≈5% increment), the loss of joint mobility and flexion (≈12-degree angle), and increased levels of pain (≈1.5 points on a scale of 0 to 3). Animals treated with GYY-4137 presented improved motor function of the joint, as well as lower pain levels (≈75% recovery). We also observed that cartilage deterioration was attenuated in the GYY-4137 group (≈30% compared with the saline group). Likewise, these animals showed a reduced presence of pro-inflammatory mediators (cyclooxygenase-2, inducible nitric oxide synthase, and metalloproteinase-13) and lower oxidative damage in the cartilage. The increment of the nuclear factor-erythroid 2-related factor 2 (Nrf-2) levels and Nrf-2-regulated gene expression (≈30%) in the GYY-4137 group seem to be underlying its chondroprotective effects. Our results suggest the beneficial impact of the intraarticular administration of H2S on experimental OA, showing a reduced cartilage destruction and oxidative damage, and supporting the use of slow H2S-producing molecules as a complementary treatment in OA.Instituto de Salud Carlos III; PI16/02124Xunta de Galicia; AGRUP2015/05 CICA-INIBICXunta de Galicia; IN607A 2017/1

Architectural Diversity of the Cuticle and Epidermis of Native and Hybrid Tomato Fruit Genotypes and the Relation to Polygalacturonase Expression

The tomato (Solanum lycopersicum L.) fruit has been widely studied because of its high consumption, nutritional value, and well-characterized genome. It also represents a good model for studying the structure and function of the cuticle, a protective film that is deposited on the surface of outer epidermal cell walls, and affects the integrity and firmness of the fruit, and, therefore, its commercialization. To evaluate the differences in their structure and function, we characterized the morphology of the cuticle and the epidermis and their relationship to firmness, brightness, weight loss, and transcript levels of polygalacturonase (PGA) in four hybrid genotypes and four native genotypes of different origins, shapes, and colors. Our results show a different architecture of the epidermis and cell wall among the genotypes. Native genotypes showed irregular-shaped epidermal cells and a thicker epidermis. Anticlinal pegs were also present to only one-third of the depth of the epidermal cells. The hybrid fruit surface was smoother compared to native genotypes, and it was associated with a higher level of brightness and a less weight loss. A negative correlation between firmness and PGA transcript levels was found. The chartreuse yellow genotype was the firmest, and it had the lowest levels of PGA expression. Meanwhile, the black genotype displayed the lowest weight loss and stem scar diameter. Our results suggest that several morphological features of the cuticle and epidermis have been modified through selection of the tomato fruit, altering quality parameters, such as weight loss and firmness

Neurocognitive Function in Acromegaly after Surgical Resection of GH-Secreting Adenoma versus Naïve Acromegaly

Patients with active untreated acromegaly show mild to moderate neurocognitive disorders that are associated to chronic exposure to growth hormone (GH) and insulin-like growth factor (IGF-I) hypersecretion. However, it is unknown whether these disorders improve after controlling GH/IGF-I hypersecretion. The aim of this study was to compare neurocognitive functions of patients who successfully underwent GH-secreting adenoma transsphenoidal surgery (cured patients) with patients with naive acromegaly. In addition, we wanted to determine the impact of different clinical and biochemical variables on neurocognitive status in patients with active disease and after long-term cure. A battery of six standardized neuropsychological tests assessed attention, memory and executive functioning. In addition, a quantitative electroencephalography with Low-Resolution Electromagnetic Tomography (LORETA) solution was performed to obtain information about the neurophysiological state of the patients. Neurocognitive data was compared to that of a healthy control group. Multiple linear regression analysis was also conducted using clinical and hormonal parameters to obtain a set of independent predictors of neurocognitive state before and after cure. Both groups of patients scored significantly poorer than the healthy controls on memory tests, especially those assessing visual and verbal recall. Patients with cured acromegaly did not obtain better cognitive measures than naïve patients. Furthermore memory deficits were associated with decreased beta activity in left medial temporal cortex in both groups of patients. Regression analysis showed longer duration of untreated acromegaly was associated with more severe neurocognitive complications, regardless of the diagnostic group, whereas GH levels at the time of assessment was related to neurocognitive outcome only in naïve patients. Longer duration of post-operative biochemical remission of acromegaly was associated with better neurocognitive state. Overall, this data suggests that the effects of chronic exposure to GH/IGF-I hypersecretion could have long-term effects on brain functions. © 2013 Martín-Rodríguez et al.Funding for this project was provided by an R&D grant from Novartis Oncology and the Plan Andaluz de Investigación (CTS-444). DAC was supported by the “Ramón y Cajal” program (RYC-2006-001071) of the Spanish Ministry of Science and Innovation.Peer Reviewe