Effects of elevated CO2 on grapevine (Vitis vinifera L.): Physiological and yield attributes

During the 2004, 2005 and 2006 growing seasons, physiological and anatomical leaf characteristics and productivity were studied in field-grown grapevines (Vitis vinifera L.) cv. 'Touriga Franca' under ambient (C, 365 ± 10 ppm) or elevated carbon dioxide [CO2] (E, 500 ± 16 ppm) under Open-top chambers (OTC-C and OTC-E, respectively). The elevated [CO2] concentration increased net photosynthetic rate (A), intrinsic water use efficiency (A/gs), leaf thickness, Mg concentration, C/N, K/N and Mg/N ratios and decreased stomatal density and N concentration. Nevertheless, stomatal conductance (gs), transpiration rate (E), photochemical efficiency (Fv/Fm), leaf water potential, SPAD-values and Red/Far-red ratio transmitted by leaves were not significantly affected by [CO2]. Meanwhile, there is no evidence for downward acclimation of photosynthesis and stomatal conductance. Yield, cluster weight and vigour showed an increase in elevated [CO2] treatment but yield to pruning mass ratio was unaffected. Despite elevated [CO2] stimulates grapevine photosynthesis and yield, more long-term studies, particularly at sub-optimal nutrient and water availability, are needed in order to reveal the grapevine responses to climate change in the Mediterranean area.

Rendimento de tecidos não componentes da carcaça de cordeiros alimentados com glicerina bruta.

Objetivou-se neste trabalho avaliar os efeitos dos níveis de inclusão de glicerina bruta (GB) na dieta cordeiros em terminação sobre o rendimento dos não componentes da carcaça

Efeitos da rega superficial e subsuperficial sobre a fisiologia e produção da variedade ‘Godello’ na Galiza, Noroeste de Espanha

Irrigation has been considered a controversial practice in European traditional viticulture due to potential alterations in the balance between vegetative growth and yield. In this regard, the influence of surface (DI) and subsurface (SDI) drip irrigation on physiological performance of the ‘Godello’ grapevine (Vitis vinifera L.) cultivar was compared with a non-irrigated control (R) over three consecutive growing seasons (2012- 2014) in NW Spain. Irrigation improved vine water status; R plants reached a minimum of midday leaf water potential of –1.5 MPa, whereas DI and SDI plants reached –1.3 MPa. Stomatal conductance was unaffected by irrigation as well as chlorophyll a fluorescence. However, photosynthetic pigments were present at higher concentrations in leaves from irrigated plants than in those from R plants in 2013. In addition, R plants showed higher values for the indicators of oxidative damage. No significant yield improvements were observed for irrigated plants, although the trend was to obtain slightly higher yields under irrigation in years with low rainfall amounts. This may not encourage growers to establish irrigation systems on their vineyards. However, these results may be important with the objectives of stabilizing yield from year to yearA rega tem sido considerada uma prática controversa na viticultura tradicional Europeia devido a potenciais alterações no equilíbrio entre crescimento vegetativo e rendimento. A este respeito, a influência da irrigação por gotejamento superficial (DI) e subsuperficial (SDI) sobre o desempenho fisiológico da variedade ‘Godello’ (Vitis vinifera L.) foi comparada com uma testemunha em condições de sequeiro (R) ao longo de três anos consecutivos (2012-2014) no noroeste de Espanha. A rega melhorou o estado hídrico da videira; as plantas do tratamento R atingiram potenciais hídricos foliares ao meio-dia de –1.5 MPa, comparativamente a um valor de –1.3 MPa para as plantas dos tratamentos DI e SDI. A conductância estomática e a fluorescência da clorofila a não foram afectadas pela rega. No entanto, os pigmentos fotossintéticos apresentaram maiores concentrações em folhas de plantas regadas quando se comparam com as plantas do tratamento R em 2013. Além disso, as plantas do tratamento R apresentaram valores superiores para os indicadores de danos oxidativos. Não foi detectado nenhum incremento do rendimento nas plantas regadas, embora se tenha observado uma tendência para obtenção de rendimentos ligeiramente mais elevados sob rega em anos com baixa pluviosidade. Tal poderá não encorajar aos viticultores a implementar sistemas de rega nas suas vinhas. No entanto, estes resultados podem ser importantes para a estabilização do rendimento de ano para anoThis research was supported by the Spanish Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Project nº RTA2011-00041-C02-00, with 80% FEDER funds. J.M. Mirás-Avalos and E.M. Martínez thank Xunta de Galicia for funding their contracts through the “Isidro Parga Pondal” and “Isabel Barreto” Programmes. E. Trigo-Córdoba thanks INIA for their PhD scholarship (FPI-INIA)S

Estudos anatómicos e histológicos em folhas de roseira desenvolvidas em diferentes condições de humidade relativa

High relative air humidity (RH > 85%) during growth has a negative impact on postharvest longevity of cut roses, being described a different susceptibility among cultivars. In this study, we evaluated the effect of RH on leaf anatomical and histological characteristics in six genotypes from a cut rose segregating tetraploid population (four sensitive and two with intermediate response to desiccation) grown at moderate (60%) or high (90%) RH. Leaves developed at high RH showed larger specific leaf area (increasing 12.8% and 26.8% on the sensitive and intermediate genotypes, respectively) and lower succulence (reducing 9.5% on the sensitive and 10.7% on the intermediate genotypes). Moreover, these leaves also showed a reduced thickness of the total lamina, cuticle and the upper and lower epidermis. Stomatal density was also reduced but stomatal dimensions were significantly larger at high RH. It is concluded that RH plays a very important role on the modification of leaf structure.A humidade relativa elevada (HR > 85%) durante o cultivo de rosas de corte tem implicações negativas na longevidade pós-colheita, estando descritas diferentes suscetibilidades entre cultivares. No presente estudo foi avaliado o efeito da HR ao nível das características anatómicas e histológicas das folhas em seis genótipos pertencentes a uma população segregante tetraploide de rosas de corte (quatro sensíveis e dois com resposta intermédia à desidratação) cultivados em HR moderada (60%) ou elevada (90%). Folhas desenvolvidas a HR elevada apresentaram uma maior área foliar específica (aumentos de 12,8% e 26,8% nos genótipos sensíveis e intermédios, respetivamente) e menor suculência (redução em 9,5 % nos genótipos sensíveis e 10,7% nos genótipos intermédios). Verificou-se igualmente nestas condições um decréscimo da espessura da lâmina total, da cutícula, da epiderme de ambas as faces e da densidade estomática, embora se tenha observado um aumento significativo das dimensões do aparelho estomático. Conclui-se que a HR tem um papel determinante na modificação da estrutura foliar

Insights into protein-ionic liquid interactions aiming at macromolecule delivery systems

Over the last few years, researchers have started to explore a particular class of compounds defined as ionic liquids (ILs) in attempts to use their unique characteristics. Since ILs have a very low vapor pressure, these fascinating compounds hold great potential as high performance chemicals for several applications in the (bio)pharmaceutical industry. In general, and unlike common organic solvents with comparable polarities, ILs are quite compatible with enzymes (enhancing their structural and chemical stability) and other proteins, since they can promote higher selectivities, faster reaction rates and greater enzyme stabilities in biocatalytic reactions providing, at the same time, a path for the structural and functional stabilization of protein entities. ILs appear to enhance the delivery of macromolecules, particularly protein entities, and their interactions with ILs will be tackled in detail in this review paper.Project funding by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, São Paulo, Brazil) (FAPESP Refs. No. 2016/08884-3 (Project PneumoPhageColor) and 2016/12234-4 (Project TransAppIL)) is hereby gratefully acknowledged. Funding by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP Ref. No. 2016/16641-3) in the form of an MSc fellowship granted to Liliam Harada is hereby gratefully acknowledged. This work also received support from CNPq, National Council for Scientific and Technological Development, Brazil, in the form of Research Productivity (PQ) fellowships granted to Victor M. Balcão (Refs. No. 306113/2014-7 and 308208/2017-0).info:eu-repo/semantics/publishedVersio

Climate-induced changes in grapevine yield and must sugar content in Franconia (Germany) between 1805 and 2010

When attempting to estimate the impacts of future climate change it is important to reflect on information gathered during the past. Understanding historical trends may also aid in the assessment of likely future agricultural and horticultural changes. The timing of agricultural activities, such as grape harvest dates, is known to be influenced by climate and weather. However, fewer studies have been carried out on grapevine yield and quality. In this paper an analysis is undertaken of long-term data from the period 1805-2010 on grapevine yield (hl/ha) and must sugar content (°Oe) and their relation to temperature. Monthly mean temperatures were obtained for the same time period. Multiple regression was used to relate the viticulture variables to temperature, and long-term trends were calculated. Overall, the observed trends over time are compatible with results from other long term studies. The findings confirm a relationship between yield, must sugar content and temperature data; increased temperatures were associated with higher yields and higher must sugar content. However, the potential increase in yield is currently limited by legislation, while must sugar content is likely to further increase with rising temperatures

Integrated analysis of climate, soil, topography and vegetative growth in Iberian viticultural regions

The Iberian viticultural regions are convened according to the Denomination of Origin (DO) and present different climates, soils, topography and management practices. All these elements influence the vegetative growth of different varieties throughout the peninsula, and are tied to grape quality and wine type. In the current study, an integrated analysis of climate, soil, topography and vegetative growth was performed for the Iberian DO regions, using state-of-the-art datasets. For climatic assessment, a categorized index, accounting for phenological/thermal development, water availability and grape ripening conditions was computed. Soil textural classes were established to distinguish soil types. Elevation and aspect (orientation) were also taken into account, as the leading topographic elements. A spectral vegetation index was used to assess grapevine vegetative growth and an integrated analysis of all variables was performed. The results showed that the integrated climate-soil-topography influence on vine performance is evident. Most Iberian vineyards are grown in temperate dry climates with loamy soils, presenting low vegetative growth. Vineyards in temperate humid conditions tend to show higher vegetative growth. Conversely, in cooler/warmer climates, lower vigour vineyards prevail and other factors, such as soil type and precipitation acquire more important roles in driving vigour. Vines in prevailing loamy soils are grown over a wide climatic diversity, suggesting that precipitation is the primary factor influencing vigour. The present assessment of terroir characteristics allows direct comparison among wine regions and may have great value to viticulturists, particularly under a changing climate

The institutions of archaic post-modernity and their organizational and managerial consequences: The case of Portugal

The long march of modernization of the Western societies tends to be presented as following a regular sequence: societies and institutions were pre-modern, and then they were modernized, eventually becoming post-modern. Such teleology may provide an incomplete or distorted narrative of societal evolution in many parts of the world, even in the ‘post-modern heartland’ of Western Europe, with Portugal being a case in point. The concept of archaic post-modernity has been developed by a philosopher, José Gil, to show how Portuguese institutions and organizations combine elements of pre-modernity and post-modernity. The notion of an archaic post-modernity is advanced in order to provide an alternative account of the modernization process, which enriches discussion of the varieties of capitalism. Differences in historical experiences create singularities that may be considered in the analysis of culture, management and organization

Centrioles: active players or passengers during mitosis?

Centrioles are cylinders made of nine microtubule (MT) triplets present in many eukaryotes. Early studies, where centrosomes were seen at the poles of the mitotic spindle led to their coining as “the organ for cell division”. However, a variety of subsequent observational and functional studies showed that centrosomes might not always be essential for mitosis. Here we review the arguments in this debate. We describe the centriole structure and its distribution in the eukaryotic tree of life and clarify its role in the organization of the centrosome and cilia, with an historical perspective. An important aspect of the debate addressed in this review is how centrioles are inherited and the role of the spindle in this process. In particular, germline inheritance of centrosomes, such as their de novo formation in parthenogenetic species, poses many interesting questions. We finish by discussing the most likely functions of centrioles and laying out new research avenues