Exogenous application of platelet-leukocyte gel during open subacromial decompression contributes to improved patient outcome

Background: Platelet-leukocyte gel (PLG) is being used during various surgical procedures in an attempt to enhance the healing process. We studied the effects of PLG on postoperative recovery of patients undergoing open subacromial decompression (OSD). Methods: PLG was produced from platelet-leukocyte-rich plasma (P-LRP), prepared from a unit of whole blood. Forty patients were included in the study. Self-assessed evaluations, using the American Shoulder and Elbow Surgeons scoring system of activities of daily living (ADL), joint instability, pain levels, pain medications, and clinical evaluations for range of motion were conducted. Results: Platelet and leukocyte counts were significantly increased in the P-LRP compared to baseline counts. Treated patients demonstrated decreased visual analog scales for pain and used significantly less pain medication, had an improved range of motion during passive forward elevation, external rotation, external rotation with arm at 90 degrees abduction, internal rotation, and cross body adduction compared to control patients (p < 0.001). No differences in the instability score were observed between the groups. Furthermore, treated patients performed more ADL (p < 0.05). Conclusion: In the PLG-treated group, recovery was faster and patients returned earlier to daily activities and also took less pain medication than control subjects

Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84

The localization of chromosome 18 in human interphase nuclei is demonstrated by use of radioactive and nonradioactive in situ hybridization techniques with a DNA clone designated L1.84. This clone represents a distinct subpopulation of the repetitive human alphoid DNA family, located in the centric region of chromosome 18. Under stringent hybridization conditions hybridization of L1.84 is restricted to chromosome 18 and reflects the number of these chromosomes present in the nuclei, namely, two in normal diploid human cells and three in nuclei from cells with trisomy 18. Under conditions of low stringency, cross-hybridization with other subpopulations of the alphoid DNA family occurs in the centromeric regions of the whole chromosome complement, and numerous hybridization sites are detected over interphase nuclei. Detection of chromosome-specific target DNAs by non-radioactive in situ hybridization with appropriate DNA probes cloned from individual chromosomal subregions presents a rapid means of identifying directly numerical or even structural chromosome aberrations in the interphase nucleus. Present limitations and future applications of interphase cytogenetics are discussed

Citizens’ opinions on (non-)essential uses of persistent chemicals: A survey in seven European countries

In accordance with the European Chemicals Strategy for Sustainability towards a Toxic-free Environment, the European Commission plans to phase out persistent chemicals, such as per- and polyfluoroalkyl substances (PFASs), except for uses classified essential for society. Until now, empirical research on what is considered an essential or non-essential chemical use has been lacking. Furthermore, as such criteria are bound to be subjective, different parties can have different views. In this study we explored which uses of persistent chemicals citizens from seven EU countries consider (non-)essential for society. As EU citizens are directly impacted by policy decisions based on (non-)essentiality criteria, we also investigated the influence of emphasis on the consequences of banning vs. allowing persistent chemicals, the association with demographics, and of having heard of persistent chemicals or PFAS prior to the study. We found substantial variation in essentiality ratings within and between use categories and between countries. Uses related to safety were frequently considered essential, whereas uses related to recreation, household, and personal care were frequently considered non-essential. Emphasis on different consequences did not influence essentiality ratings. Gender, age, education, and political orientation were to some extent associated with essentiality ratings. People who had not heard of persistent chemicals or PFAS prior to the study rated uses of persistent chemicals less frequently as non-essential or essential. Our findings offer insight into EU citizens’ opinions on (non-)essential uses of persistent chemicals, and provide empirical input to the scientific and public debate on framing the concept of essential use.</p

Inhibition of succinate dehydrogenase dysregulates histone modification in mammalian cells

Remodelling of mitochondrial metabolism is a hallmark of cancer. Mutations in the genes encoding succinate dehydrogenase (SDH), a key Krebs cycle component, are associated with hereditary predisposition to pheochromocytoma and paraganglioma, through mechanisms which are largely unknown. Recently, the jumonji-domain histone demethylases have emerged as a novel family of 2-oxoglutarate-dependent chromatin modifiers with credible functions in tumourigenesis. Using pharmacological and siRNA methodologies we show that increased methylation of histone H3 is a general consequence of SDH loss-of-function in cultured mammalian cells and can be reversed by overexpression of the JMJD3 histone demethylase. ChIP analysis revealed that the core promoter of IGFBP7, which encodes a secreted protein upregulated after loss of SDHB, showed decreased occupancy by H3K27me3 in the absence of SDH. Finally, we provide the first evidence that the chief (type I) cell is the major methylated histone-immunoreactive constituent of paraganglioma. These results support the notion that loss of mitochondrial function alters epigenetic processes and might provide a signature methylation mark for paraganglioma

Codificatie of zelfregulering in de franchisesector?

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Codificatie of zelfregulering in de franchisesector?

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