633 research outputs found
Fractional smoothness and applications in finance
This overview article concerns the notion of fractional smoothness of random
variables of the form , where is a certain
diffusion process. We review the connection to the real interpolation theory,
give examples and applications of this concept. The applications in stochastic
finance mainly concern the analysis of discrete time hedging errors. We close
the review by indicating some further developments.Comment: Chapter of AMAMEF book. 20 pages
Actin assembly ruptures the nuclear envelope by prying the lamina away from nuclear pores and nuclear membranes in starfish oocytes.
The nucleus of oocytes (germinal vesicle) is unusually large and its nuclear envelope (NE) is densely packed with nuclear pore complexes (NPCs) stockpiled for embryonic development. We showed that breakdown of this specialized NE is mediated by an Arp2/3-nucleated F-actin 'shell' in starfish oocytes, in contrast to microtubule-driven tearing in mammalian fibroblasts. Here, we address the mechanism of F-actin-driven NE rupture by correlated live-cell, super-resolution and electron microscopy. We show that actin is nucleated within the lamina sprouting filopodia-like spikes towards the nuclear membranes. These F-actin spikes protrude pore-free nuclear membranes, whereas the adjoining membrane stretches accumulate NPCs associated with the still-intact lamina. Packed NPCs sort into a distinct membrane network, while breaks appear in ER-like, pore-free regions. Thereby, we reveal a new function for actin-mediated membrane shaping in nuclear rupture that is likely to have implications in other contexts such as nuclear rupture observed in cancer cells
Recombination-ready Sindbis replicon expression vectors for transgene expression
<p>Abstract</p> <p>Background</p> <p>Sindbis viruses have been widely used as tools to study gene function in cells. Despite the utility of these systems, the construction and production of alphavirus replicons is time consuming and inefficient due to potential additional restriction sites within the insert region and lack of directionality for insert ligation. In this report, we present a system useful for producing recombinant Sindbis replicons that uses lambda phage recombination technology to rapidly and specifically construct replicon expression plasmids that contain insert regions in the desired orientation.</p> <p>Results</p> <p>Recombination of the gene of interest with the replicon plasmid resulted in nearly 100% recombinants, each of which contained a correctly orientated insert. Replicons were easily produced in cell culture and packaged into pseudo-infectious viral particles. Insect and mammalian cells infected with pseudo-infectious viral particles expressed various transgenes at high levels. Finally, inserts from persistently replicating replicon RNA were easily isolated and recombined back into entry plasmids for sequencing and subsequent analysis.</p> <p>Conclusion</p> <p>Replication-ready replicon expression plasmids make the use of alphavirus replicons fast and easy as compared to traditional replicon production methods. This system represents a significant step forward in the utility and ease of use of alphavirus replicons in the study of gene function.</p
What is the benefit of ceilometers for aerosol remote sensing? An answer from EARLINET
With the establishment of ceilometer networks by national weather services, a discussion commenced to which extent these simple backscatter lidars can be used for aerosol research. Though primarily designed for the detection of clouds it was shown that at least observations of the vertical structure of the boundary layer might be possible. However, an assessment of the potential of ceilometers for the quantitative retrieval of aerosol properties is still missing. In this paper we discuss different retrieval methods to derive the aerosol backscatter coefficient beta(p),with special focus on the calibration of the ceilometers. Different options based on forward and backward integration methods are compared with respect to their accuracy and applicability. It is shown that advanced lidar systems such as those being operated in the framework of the European Aerosol Research Lidar Network (EARLINET) are excellent tools for the calibration, and thus beta(p) retrievals based on forward integration can readily be implemented and used for real-time applications. Furthermore, we discuss uncertainties introduced by incomplete overlap, the unknown lidar ratio, and water vapor absorption. The latter is relevant for the very large number of ceilometers operating in the spectral range around lambda = 905-910 nm. The accuracy of the retrieved beta(p) mainly depends on the accuracy of the calibration and the long-term stability of the ceilometer. Under favorable conditions, a relative error of beta(p) on the order of 10% seems feasible. In the case of water vapor absorption, corrections assuming a realistic water vapor distribution and laser spectrum are indispensable;otherwise errors on the order of 20% could occur. From case studies it is shown that ceilometers can be used for the reliable detection of elevated aerosol layers below 5 km, and can contribute to the validation of chemistry transport models, e. g.,the height of the boundary layer. However, the exploitation of ceilometer measurements is still in its infancy, so more studies are urgently needed to consolidate the present state of knowledge, which is based on a limited number of case studies
The Intrinsic Fundamental Group of a Linear Category
We provide an intrinsic definition of the fundamental group of a linear
category over a ring as the automorphism group of the fibre functor on Galois
coverings. If the universal covering exists, we prove that this group is
isomorphic to the Galois group of the universal covering. The grading deduced
from a Galois covering enables us to describe the canonical monomorphism from
its automorphism group to the first Hochschild-Mitchell cohomology vector
space.Comment: Final version, to appear in Algebras and Representation Theor
Our Sun. IV. The Standard Model and Helioseismology: Consequences of Uncertainties in Input Physics and in Observed Solar Parameters
Helioseismology provides a powerful tool to explore the deep interior of the
Sun: for example, the adiabatic sound speed can be inferred with an accuracy of
a few parts in 10,000. This has become a serious challenge to theoretical
models of the Sun. Therefore, we have undertaken a self-consistent, systematic
study of sources of uncertainties in the standard solar model, which must be
understood before the helioseismic observations can be used as constraints on
theory. We find that the largest uncertainty in the sound speed in the solar
interior, namely, 3 parts in 1000, arises from uncertainties in the observed
photospheric abundances of the elements; uncertainties of 1 part in 1000 arise
from (1) the 4% uncertainty in the OPAL opacities, (2) the 5% uncertainty in
the basic pp nuclear reaction rate, (3) the 15% uncertainty in the diffusion
constants for the gravitational settling of helium, and (4) the 50%
uncertainties in diffusion constants for the heavier elements. (Other
investigators have shown that similar uncertainties arise from uncertainties in
the interior equation of state and in rotation-induced turbulent mixing.) The
predicted pre-main-sequence solar lithium depletion is a factor of order 20 (an
order of magnitude larger than that predicted by earlier models that neglected
gravitational settling and used older opacities), and is uncertain by a factor
of 2. The predicted neutrino capture rate is uncertain by 30% for the Cl-37
experiment and by 3% for the Ga-71 experiments (not including uncertainties in
the capture cross sections), while the B-8 neutrino flux is uncertain by 30%.Comment: LaTeX, 38 pages (including 8 figures); ApJ, in press. Added
figures/color figurea available at
http://www.cita.utoronto.ca/~boothroy/sun4.htm
Dust detection by the wave instrument on STEREO: nanoparticles picked up by the solar wind?
The STEREO/WAVES instrument has detected a very large number of intense
voltage pulses. We suggest that these events are produced by impact ionisation
of nanoparticles striking the spacecraft at a velocity of the order of
magnitude of the solar wind speed. Nanoparticles, which are half-way between
micron-sized dust and atomic ions, have such a large charge-to-mass ratio that
the electric field induced by the solar wind magnetic field accelerates them
very efficiently. Since the voltage produced by dust impacts increases very
fast with speed, such nanoparticles produce signals as high as do much larger
grains of smaller speeds. The flux of 10-nm radius grains inferred in this way
is compatible with the interplanetary dust flux model. The present results may
represent the first detection of fast nanoparticles in interplanetary space
near Earth orbit.Comment: In press in Solar Physics, 13 pages, 5 figure
Origin and evolution of the light nuclides
After a short historical (and highly subjective) introduction to the field, I
discuss our current understanding of the origin and evolution of the light
nuclides D, He-3, He-4, Li-6, Li-7, Be-9, B-10 and B-11. Despite considerable
observational and theoretical progress, important uncertainties still persist
for each and every one of those nuclides. The present-day abundance of D in the
local interstellar medium is currently uncertain, making it difficult to infer
the recent chemical evolution of the solar neighborhood. To account for the
observed quasi-constancy of He-3 abundance from the Big Bang to our days, the
stellar production of that nuclide must be negligible; however, the scarce
observations of its abundance in planetary nebulae seem to contradict this
idea. The observed Be and B evolution as primaries suggests that the source
composition of cosmic rays has remained quasi-constant since the early days of
the Galaxy, a suggestion with far reaching implications for the origin of
cosmic rays; however, the main idea proposed to account for that constancy,
namely that superbubbles are at the source of cosmic rays, encounters some
serious difficulties. The best explanation for the mismatch between primordial
Li and the observed "Spite-plateau" in halo stars appears to be depletion of Li
in stellar envelopes, by some yet poorly understood mechanism. But this
explanation impacts on the level of the recently discovered early ``Li-6
plateau'', which (if confirmed), seriously challenges current ideas of cosmic
ray nucleosynthesis.Comment: 18 pages, 9 figs. Invited Review in "Symposium on the Composition of
Matter", honoring Johannes Geiss on the occasion of his 80th birthday
(Grindelwald, Switzerland, Sept. 2006), to be published in Space Science
Series of ISS
Constraints from deuterium on the formation of icy bodies in the Jovian system and beyond
We consider the role of deuterium as a potential marker of location and
ambient conditions during the formation of small bodies in our Solar system. We
concentrate in particular on the formation of the regular icy satellites of
Jupiter and the other giant planets, but include a discussion of the
implications for the Trojan asteroids and the irregular satellites. We examine
in detail the formation of regular planetary satellites within the paradigm of
a circum-Jovian subnebula. Particular attention is paid to the two extreme
potential subnebulae - "hot" and "cold". In particular, we show that, for the
case of the "hot" subnebula model, the D:H ratio in water ice measured from the
regular satellites would be expected to be near-Solar. In contrast, satellites
which formed in a "cold" subnebula would be expected to display a D:H ratio
that is distinctly over-Solar. We then compare the results obtained with the
enrichment regimes which could be expected for other families of icy small
bodies in the outer Solar system - the Trojan asteroids and the irregular
satellites. In doing so, we demonstrate how measurements by Laplace, the James
Webb Space Telescope, HERSCHEL and ALMA will play an important role in
determining the true formation locations and mechanisms of these objects.Comment: Accepted and shortly to appear in Planetary and Space Science; 11
pages with 5 figure
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