GENETIC PERSPECTIVES ON BIODIVERSITY IN ROCKY MOUNTAIN ALPINE STREAMS

In alpine regions worldwide, climate change is dramatically altering ecosystems, affecting biodiversity across habitats and taxonomic scales. For streams, the associated recession of mountain glaciers and snowfields, paired with altered precipitation regimes, are driving shifts in hydrology, species distributions, and basal resources – often threatening the very existence of some habitats and biota. Globally, alpine streams harbor particularly substantial species and genetic diversity due to significant habitat insularity and environmental heterogeneity: however, anthropogenic warming threatens to homogenize habitats through the reduction of the cryosphere, thereby reducing biodiversity from micro- to macroscopic organisms and genes to communities. Still, alpine stream biodiversity, particularly in North America, is poorly understood, making it difficult to predict future changes without baselines for comparison. For my dissertation, I used genetic tools to assess biodiversity in alpine streams of the central Rocky Mountains in North America. Here, I begin by reviewing the current state of alpine stream biology from an organismal perspective. Next, I provide two perspectives on macroinvertebrate diversity. The first, a population genetic comparison of three highly similar species, is followed by a fine-scale genomic study of one species, Lednia tumana. I follow these largely macroinvertebrate-centric chapters with a modern synthesis of the microbial ecology of mountain glacier ecosystems. Finally, I conclude with a study of microbial diversity that addresses how microbial diversity is shaped by geography, habitat, and hydrological source in North America. Collectively, this research refines existing themes in alpine stream biology by revealing unexpected differences in population genetic patterns among closely related species, the influence of recent deglaciation on population genetic structure and demographic history of a threatened stonefly, and clarification of the environmental drivers shaping microbial diversity

Rock Glaciers in Utah

Utah’s primary water supply––winter snowpack––is in decline due to climate warming coupled with more precipitation falling as rain instead of snow. As snowpack dwindles, other sources of cold stream water, such as rock glaciers, will become more important. Rock glaciers contain significant volumes of internal ice covered by debris. This internal ice provides cold meltwater to mountain streams, sustaining flows in summer and supporting biodiversity. Rock glaciers are common in Utah’s mountains and are projected to be more stable under climate change than Utah’s snowpack. Thus, rock glaciers are likely to persist in their current form even as snowpack volumes continue to decrease. Because rock glaciers may provide a critical, climate-resilient water source for Utah, more research is needed to quantify their relevance for present and future water availability and ecosystems

Long-term trends in aquatic ecosystem bioassessment metrics are not influenced by sampling method: empirical evidence from the Niobrara River

Choosing an aquatic invertebrate sampling method for biomonitoring depends upon study goals, resources, and ecosystem conditions. In this study, we compared two methods that are widely used in stream ecology, but have not been directly compared: Hester–Dendy (HD) and Hess sampling. Hester–Dendy sampling uses artificial substrate that invertebrates colonize over a specific period of time. In contrast, Hess samplers surround a fixed area of natural substrate with a net. To compare approaches, we combined 5 years of simultaneous HD and Hess data collection (2010–2014) from the Niobrara River with a 14-year (1996–2009) historical HD data set for the same study sites. We used this full 19-year data set to assess how ecosystem health has changed in the Niobrara River over time, while also testing the influence of HD versus Hess data (2010–2014) on historical trends (1996–2009). Our results showed that HD samples are more taxonomically variable and bias bioassessment metrics because they collect more sensitive taxa versus Hess sampling. However, when combined with the 1996–2009 HD data set, both recent HD and Hess data sets recovered the same trend of declining ecosystem health in the Niobrara River. These results provide empirical evidence that even when historical HD data are combined with recent Hess data, long-term bioassessment trends remain unchanged despite more accurate perspectives of invertebrate assemblages being collected

Near-surface hydraulic conductivity of Northern Hemisphere glaciers

The hydrology of near‐surface glacier ice remains a neglected aspect of glacier hydrology despite its role in modulating meltwater delivery to downstream environments. To elucidate the hydrological characteristics of this near‐surface glacial weathering crust, we describe the design and operation of a capacitance‐based piezometer that enables rapid, economical deployment across multiple sites and provides an accurate, high‐resolution record of near‐surface water‐level fluctuations. Piezometers were employed at 10 northern hemisphere glaciers, and through the application of standard bail–recharge techniques, we derive hydraulic conductivity (K) values from 0.003 to 3.519 m day−1, with a mean of 0.185 ± 0.019 m day−1. These results are comparable to those obtained in other discrete studies of glacier near‐surface ice, and for firn, and indicate that the weathering crust represents a hydrologically inefficient aquifer. Hydraulic conductivity correlated positively with water table height but negatively with altitude and cumulative short‐wave radiation since the last synoptic period of either negative air temperatures or turbulent energy flux dominance. The large range of K observed suggests complex interactions between meteorological influences and differences arising from variability in ice structure and crystallography. Our data demonstrate a greater complexity of near‐surface ice hydrology than hitherto appreciated and support the notion that the weathering crust can regulate the supraglacial discharge response to melt production. The conductivities reported here, coupled with typical supraglacial channel spacing, suggest that meltwater can be retained within the weathering crust for at least several days. Not only does this have implications for the accuracy of predictive meltwater run‐off models, but we also argue for biogeochemical processes and transfers that are strongly conditioned by water residence time and the efficacy of the cascade of sediments, impurities, microbes, and nutrients to downstream ecosystems. Because continued atmospheric warming will incur rising snowline elevations and glacier thinning, the supraglacial hydrological system may assume greater importance in many mountainous regions, and consequently, detailing weathering crust hydraulics represents a research priority because the flow path it represents remains poorly constrained

Male Oxidative Stress Infertility (MOSI): Proposed Terminology and Clinical Practice Guidelines for Management of Idiopathic Male Infertility

Despite advances in the field of male reproductive health, idiopathic male infertility, in which a man has altered semen characteristics without an identifiable cause and there is no female factor infertility, remains a challenging condition to diagnose and manage. Increasing evidence suggests that oxidative stress (OS) plays an independent role in the etiology of male infertility, with 30% to 80% of infertile men having elevated seminal reactive oxygen species levels. OS can negatively affect fertility via a number of pathways, including interference with capacitation and possible damage to sperm membrane and DNA, which may impair the sperm’s potential to fertilize an egg and develop into a healthy embryo. Adequate evaluation of male reproductive potential should therefore include an assessment of sperm OS. We propose the term Male Oxidative Stress Infertility, or MOSI, as a novel descriptor for infertile men with abnormal semen characteristics and OS, including many patients who were previously classified as having idiopathic male infertility. Oxidation-reduction potential (ORP) can be a useful clinical biomarker for the classification of MOSI, as it takes into account the levels of both oxidants and reductants (antioxidants). Current treatment protocols for OS, including the use of antioxidants, are not evidence-based and have the potential for complications and increased healthcare-related expenditures. Utilizing an easy, reproducible, and cost-effective test to measure ORP may provide a more targeted, reliable approach for administering antioxidant therapy while minimizing the risk of antioxidant overdose. With the increasing awareness and understanding of MOSI as a distinct male infertility diagnosis, future research endeavors can facilitate the development of evidence-based treatments that target its underlying cause

Recent developments in genetics and medically assisted reproduction : from research to clinical applications

Two leading European professional societies, the European Society of Human Genetics and the European Society for Human Reproduction and Embryology, have worked together since 2004 to evaluate the impact of fast research advances at the interface of assisted reproduction and genetics, including their application into clinical practice. In September 2016, the expert panel met for the third time. The topics discussed highlighted important issues covering the impacts of expanded carrier screening, direct-to-consumer genetic testing, voiding of the presumed anonymity of gamete donors by advanced genetic testing, advances in the research of genetic causes underlying male and female infertility, utilisation of massively parallel sequencing in preimplantation genetic testing and non-invasive prenatal screening, mitochondrial replacement in human oocytes, and additionally, issues related to cross-generational epigenetic inheritance following IVF and germline genome editing. The resulting paper represents a consensus of both professional societies involved.Peer reviewe

Lawson criterion for ignition exceeded in an inertial fusion experiment

For more than half a century, researchers around the world have been engaged in attempts to achieve fusion ignition as a proof of principle of various fusion concepts. Following the Lawson criterion, an ignited plasma is one where the fusion heating power is high enough to overcome all the physical processes that cool the fusion plasma, creating a positive thermodynamic feedback loop with rapidly increasing temperature. In inertially confined fusion, ignition is a state where the fusion plasma can begin "burn propagation" into surrounding cold fuel, enabling the possibility of high energy gain. While "scientific breakeven" (i.e., unity target gain) has not yet been achieved (here target gain is 0.72, 1.37 MJ of fusion for 1.92 MJ of laser energy), this Letter reports the first controlled fusion experiment, using laser indirect drive, on the National Ignition Facility to produce capsule gain (here 5.8) and reach ignition by nine different formulations of the Lawson criterion