TempoNetze - Kooperationsverbünde auf Zeit

Durch Bildung temporärer Netzwerke soll eine zeitgemäße und effizientere Form des E-Learning-Supports an den Hochschulen in Rheinland-Pfalz aufgebaut werden, die einen höchstmöglichen Synergieeffekt für alle Beteiligten generiert. Neu an diesem Ansatz ist die Berücksichtigung, Einbeziehung und Beteiligung aller Hochschulen im Land (Universitäten, Fachhochschulen, DHV) mit dem Virtuellen Campus Rheinland-Pfalz (VCRP) als zentrale Koordinierungsstelle. Der Beitrag beschreibt sowohl die Hintergründe, das Konzept als auch das Vorgehen das mit dem Aufbau der TempoNetze verbunden ist. 23.03.2010 | Simone Grimmig (Kaiserslautern), Sabine Hemsing (Kaiserslautern) & Dorothea Hoppe-Dörwald (Worms

Estágio apoiado: uma perspectiva de formação profissional inclusiva

Supported internship: an inclusive professional formation perspectiveResumoEste artigo aborda a estratégia de Estágio Apoiado na formação profissional de nível médio. O objetivo do estágio foi de promover a inserção institucional de uma estudante com deficiência intelectual e nanismo. O estudo teve por contexto uma instituição de educação profissional que recebe estudantes com necessidades educacionais específicas e objetivou a análise da acessibilidade e do apoio para a melhoria das condições de aprendizagem destes estudantes. Foi utilizada uma metodologia de estudo de caso único (YIN, 2010; STAKE, 2007), com observações participantes e entrevistas com a estudante e seus familiares. O resultado da investigação revelou a importância da identificação da resistência no processo de aprendizagem e da intervenção nesse momento pedagógico com as ferramentas específicas propostas por Meirieu (2002). O Estágio Apoiado possibilitou, para a estudante, o exercício das potencialidades e da autonomia em novas interações. Palavras-chave: Educação profissional. Inclusão. Estágio Apoiado.  AbstractThis article discusses the Internship Supported strategy in professional high school formation. The aim of this internship was to promote a institutional insertion of a student with intellectual disability and dwarfism. This study had as context a professional education institution that receives students with specific educational needs and aimed to analyze the accessibility and support to improve the learning conditions of these students. A single case study methodology was used (YIN, 2010; STAKE, 2007), with participant observations and interviews with the student and her family members. The result of the research revealed the importance of identifying resistance in the learning process and the intervention in this pedagogical moment, with specific tools (MEIRIEU, 2002). The Supported Internship made it possible to exercise the student's potential and autonomy in new interactions.Keywords: Professional Education. Inclusion. Supported Internship

Three-dimensional architecture and biogenesis of membrane structures associated with hepatitis C virus replication

All positive strand RNA viruses are known to replicate their genomes in close association with intracellular membranes. In case of the hepatitis C virus (HCV), a member of the family Flaviviridae, infected cells contain accumulations of vesicles forming a membranous web (MW) that is thought to be the site of viral RNA replication. However, little is known about the biogenesis and three-dimensional structure of the MW. In this study we used a combination of immunofluorescence- and electron microscopy (EM)-based methods to analyze the membranous structures induced by HCV in infected cells. We found that the MW is derived primarily from the endoplasmic reticulum (ER) and contains markers of rough ER as well as markers of early and late endosomes, COP vesicles, mitochondria and lipid droplets (LDs). The main constituents of the MW are single and double membrane vesicles (DMVs). The latter predominate and the kinetic of their appearance correlates with kinetics of viral RNA replication. DMVs are induced primarily by NS5A whereas NS4B induces single membrane vesicles arguing that MW formation requires the concerted action of several HCV replicase proteins. Three-dimensional reconstructions identify DMVs as protrusions from the ER membrane into the cytosol, frequently connected to the ER membrane via a neck-like structure. In addition, late in infection multi-membrane vesicles become evident, presumably as a result of a stress-induced reaction. Thus, the morphology of the membranous rearrangements induced in HCV-infected cells resemble those of the unrelated picorna-, corona- and arteriviruses, but are clearly distinct from those of the closely related flaviviruses. These results reveal unexpected similarities between HCV and distantly related positive-strand RNA viruses presumably reflecting similarities in cellular pathways exploited by these viruses to establish their membranous replication factories

Enhancing the recovery of oilseed rape seed oil bodies (oleosomes) using bicarbonate-based soaking and grinding media

An aqueous process for the recovery of oil bodies from rapeseed using sodium bicarbonate-based soaking and grinding media (pH 9.5) was investigated. The effect of the ratio between seed and mass of media during grinding and molarity of the medium used on oil body integrity, purity and storage stability have been studied. The grinding of seeds in solution at a ratio of 1:7 (w/w) significantly improved the quality of oil body suspension to a size more in-line with that seen in vivo (average D4,3 of 1.19 μm). The purity and the composition of the recovered oil bodies depends on the molarity of medium used; the use of a sodium bicarbonate solution (pH 9.5, 0.1 M) in the grinding and washing steps produced oil body preparations with the same purity as that resulting from washing a crude preparation with 9 M urea. The resultant emulsion had improved physical stability over a storage period of one month

Daytime thermal comfort in urban spaces: A field study in Brazil

AbstractThis article presents the results from thermal comfort surveys in two squares located in the city of Belo Horizonte, Brazil over two different seasons. Objective environmental parameters were compared with subjective responses collected during field surveys in order to evaluate thermal comfort conditions people experience and identify potential thermal adaptation processes. Individuals and behavioral’ characteristics were also taken into account. The summer survey was carried out in March 2013 and the winter survey in July 2013, both comprising a total of 1693 interviewees. The PET index was calibrated to determine the thermally acceptable range. Neutral and preferred temperatures, for both summer and winter, were obtained in order to assess thermal preference. The results show that people were more tolerant in one of the squares (Liberdade square) in winter, considering the same thermal conditions. These findings were associated to psychological processes related to thermal adaptation, such as naturalness, perceived control, experience (thermal history on longer timescales – seasonal) and environmental diversity - along with the presence of greater adaptive opportunities. The calibration of the PET index, resulted in the definition of the thermal acceptability range of: “Cold” for PET values bellow 19 °C; “Neutral” for PET values between 19 °C and 27 °C; “Hot” for PET values greater than 27 °C. Neutral temperatures were 27.7 °C, in summer, and 15.9 °C, in winter; while preferred temperatures were 14.9 °C, in summer, and 20.9 °C, in winter. Design strategies, such as shading, exposure to the wind and providing increased environmental diversity may improve urban environments and pedestrians' experience in cities

Live well, die well – an international cohort study on experiences, concerns and preferences of patients in the last phase of life: the research protocol of the iLIVE study

Introduction Adequately addressing the needs of patients at the end of life and their relatives is pivotal in preventing unnecessary suffering and optimising their quality of life. The purpose of the iLIVE study is to contribute to high-quality personalised care at the end of life in different countries and cultures, by investigating the experiences, concerns, preferences and use of care of terminally ill patients and their families. Methods and analysis The iLIVE study is an international cohort study in which patients with an estimated life expectancy of 6 months or less are followed up until they die. In total, 2200 patients will be included in 11 countries, that is, 200 per country. In addition, one relative per patient is invited to participate. All participants will be asked to fill in a questionnaire, at baseline and after 4 weeks. If a patient dies within 6 months of follow-up, the relative will be asked to fill in a post-bereavement questionnaire. Healthcare use in the last week of life will be evaluated as well; healthcare staff who attended the patient will be asked to fill in a brief questionnaire to evaluate the care that was provided. Qualitative interviews will be conducted with patients, relatives and healthcare professionals in all countries to gain more in-depth insights. Ethics and dissemination The cohort study has been approved by ethics committees and the institutional review boards (IRBs) of participating institutes in all countries. Results will be disseminated through the project website, publications in scientific journals and at conferences. Within the project, there will be a working group focusing on enhancing the engagement of the community at large with the reality of death and dying. Trial registration number NCT04271085