ECOLOGICAL EFFECTS OF TRANSGENIC CROPS AND THE ESCAPE OF TRANSGENES INTO WILD POPULATIONS

Ecological risks associated with the release of transgenic crops include nontarget effects of the crop and the escape of transgenes into wild populations. Nontarget effects can be of two sorts: (a) unintended negative effects on species that do not reduce yield and (b) greater persistence of the crop in feral populations. Conventional agricultural methods, such as herbicide and pesticide application, have large and well- documented nontarget effects. To the extent that transgenes have more specific target effects, transgenic crops may have fewer nontarget effects. The escape of transgenes into wild populations, via hybridization and introgression, could lead to increased weediness or to the invasion of new habitats by the wild population. In addition, native species with which the wild plant interacts (including herbivores, pathogens, and other plant species in the community) could be negatively affected by “transgenic-wild” plants. Conventional crop alleles have facilitated the evolution of increased weediness in several wild populations. Thus, some transgenes that allow plants to tolerate biotic and abiotic stress (e.g., insect resistance, drought tolerance) could have similar effects

Ecological effects of virus-resistant transgenic squash

Two ecological risks associated with the use of transgenic crops include the effects of transgene products on non-target organisms and the effects of a transgene after it moves from crops into a wild plant population. In work presented here, we specifically investigate the ecological risks of virus-resistant transgenic squash. We observed pollinator behavior to determine if pollinators are affected by nontarget effects of the virus-resistant transgene. We found that pollinator behavior did differ between conventional and virus-resistant transgenic squash due to pleiotropic effects of the transgene. This difference in pollinator behavior can affect plant mating patterns, thereby affecting crop-wild hybridization and transgene introgression into wild squash populations. For the virus-resistant transgene to confer a benefit in wild squash populations virus must be present. Thus, we surveyed wild squash populations to determine the prevalence of five virus species and members of one virus genus. We found that virus is prevalent in wild squash populations though variable among populations, virus species, and years. Finally, we focused on the effects of the virus-resistant transgene in wild squash populations. Then, we surveyed wild squash populations for the virus-resistant transgene, which we did not find. Next, we found the population growth rate of wild squash is reduced by virus. However, there is no affect of virus when the virus-resistant transgene is present in wild squash. We recommend future risk assessments of transgenic crops to examine non-target effects of transgenes on pollinators in different environments as this can affect transgene movement into wild populations. Furthermore, additional wild squash populations should be assayed for the transgene, since our work was not exhaustive. Moreover, to predict when virus affects wild populations, thereby infer when a virus- resistant transgene is favored by natural selection, additional work examining plant-virus ecology is essential. The results from these studies will allow us to better predict the evolution of transgenic resistance in wild populations and guide policy decisions on the use and deregulation of transgenic crops. Adviser: Diana Pilso

VIRUS INFECTIONS IN WILD PLANT POPULATIONS ARE BOTH FREQUENT AND OFTEN UNAPPARENT

• Premise of the study: Pathogens are thought to regulate host populations. In agricultural crops, virus infection reduces yield. However, in wild plants little is known about the spatial and temporal patterns of virus prevalence. Thus, pathogen effects on plant population dynamics are unclear. Prevalence data provide necessary background for (1) evaluating the effects of virus infection on plant population size and dynamics and (2) improving risk assessment of virus-resistant transgenic crops. • Methods: We used ELISA and RT-PCR to survey wild Cucurbita pepo populations over 4 years for five viruses, aphid-transmitted viruses of the genus Potyvirus as a group and PCR to survey for virus-resistance transgenes. In addition, we surveyed the literature for reports of virus prevalence in wild populations. • Key results: In 21 C. pepo populations, virus prevalence (0–74%) varied greatly among populations, years, and virus species. In samples analyzed by both ELISA and RT-PCR, RT-PCR detected 6–44% more viruses than did ELISA. Eighty percent of these infections did not cause any visually apparent symptoms. In our samples, the virus-resistance transgene was not present. In 30 published studies, 92 of 146 tested species were infected with virus, and infection rates ranged from 0.01–100%. Most published studies used ELISA, suggesting virus prevalence is higher than reported. • Conclusions: In wild C. pepo , the demographic effects of virus are likely highly variable in space and time. Further, our literature survey suggests that such variation is probably common across plant species. Our results indicate that risk assessments for virus-resistant transgenic crops should not rely on visual symptoms or ELISA and should include data from multiple populations over multiple years

Data from: Response to joint selection on germination and flowering phenology depends on the direction of selection

Background and Aims. Flowering and germination time are components of phenology, a complex phenotype that incorporates a number of traits. In natural populations, selection is likely to occur on multiple components of phenology at once. However, we have little knowledge of how joint selection on several phenological traits influences evolutionary response. Methods. We conducted one generation of artificial selection for all combinations of early and late germination and flowering on replicated lines within two independent base populations in the herb Campanula americana. We then measured response to selection and realized heritability for each trait. Results. Response to selection and heritability were greater for flowering time than germination time, indicating greater evolutionary potential of this trait. Selection for earlier phenology, both flowering and germination, did not depend on the direction of selection on the other trait. Whereas response to selection to delay germination and flowering was greater when selection on the other trait was in the opposite direction (e.g. early germination, late flowering), indicating a negative genetic correlation between the traits. Conclusions. The extent to which correlations shaped response to selection depended on the direction of selection. Therefore the genetic correlation between timing of germination and flowering varies across the trait distributions. The negative correlation between germination and flowering time found when selecting for delayed phenology follows theoretical predictions of constraint for traits that jointly determine life history schedule. Whereas the lack of constraint found when selecting for an accelerated phenology suggests a reduction of the covariance due to strong selection favoring earlier flowering and a shorter life cycle. This genetic architecture, in turn, will facilitate further evolution of the early phenology often favored in warming climates

Appendix A. Geography and identification of Campanulastrum americanum populations in this study.

Geography and identification of Campanulastrum americanum populations in this study

Data from: Population history provides foundational knowledge for utilizing and developing native plant restoration materials

A species’ population structure and history are critical pieces of information that can help guide the use of available native plant materials in restoration treatments and decide what new native plant materials should be developed to meet future restoration needs. In the western United States, Pseudoroegneria spicata (bluebunch wheatgrass; Poaceae) is an important component of grassland and shrubland plant communities and commonly used for restoration due to its drought resistance and competitiveness with exotic weeds. We used next-generation sequencing data to investigate the processes that shaped P. spicata’s geographic pattern of genetic variation across the Intermountain West. Pseudoroegneria spicata’s genetic diversity is partitioned into populations that likely differentiated since the Last Glacial Maximum. Adjacent populations display varying magnitudes of historical gene flow, with migration rates ranging from multiple migrants per generation to multiple generations per migrant. When considering the commercial germplasm sources available for restoration, genetic identities remain representative of the wildland localities from which germplasm sources were originally developed, and they maintain high levels of heterozygosity and nucleotide diversity. However, the commercial germplasm sources represent a small fraction of the overall genetic diversity of P. spicata in the Intermountain West. Given the low migration rates and long divergence times between some pairs of P. spicata populations, using commercial germplasm sources could facilitate undesirable restoration outcomes when used in certain geographic areas, even if the environment in which the commercial materials thrive is similar to that of the restoration site. As such, population structure and history can be used to provide guidance on what geographic areas may need additional native plant materials so that restoration efforts support species and community resilience and improve outcomes

The evolution of parasitic and mutualistic plant–virus symbioses through transmission-virulence trade-offs

International audienceVirus–plant interactions range from parasitism to mutualism. Viruses have been shown to increase fecundity of infected plants in comparison with uninfected plants under certain environmental conditions. Increased fecundity of infected plants may benefit both the plant and the virus as seed transmission is one of the main virus transmission pathways, in addition to vector transmission. Trade-offs between vertical (seed) and horizontal (vector) transmission pathways may involve virulence, defined here as decreased fecundity in infected plants. To better understand plant–virus symbiosis evolution, we explore the ecological and evolutionary interplay of virus transmission modes when infection can lead to an increase in plant fecundity. We consider two possible trade-offs: vertical seed transmission vs infected plant fecundity, and horizontal vector transmission vs infected plant fecundity (virulence). Through mathematical models and numerical simulations, we show (1) that a trade-off between virulence and vertical transmission can lead to virus extinction during the course of evolution, (2) that evolutionary branching can occur with subsequent coexistence of mutualistic and parasitic virus strains, and (3) that mutualism can out-compete parasitism in the long-run. In passing, we show that ecological bi-stability is possible in a very simple discrete-time epidemic model. Possible extensions of this study include the evolution of conditional (environment-dependent) mutualism in plant viruses