Detection of new pulsations in the roAp star HD 177765

We report the discovery of 2 previously undetected pulsation frequencies in the known roAp star HD 177765. Photometric observations by the Kepler space telescope during K2 Campaign 7 show low-amplitude pulsations (4-11 micro mag) previously unseen in photometry. We show the pulsations to be stable over the observation period, and demonstrate that the separation of the frequencies is not representative of the large frequency separation quantity needed to perform asteroseismic analysis

Pulsating stars in SuperWASP

Abstract. SuperWASP is one of the largest ground-based surveys for transiting exoplan- ets. To date, it has observed over 31 million stars. Such an extensive database of time resolved photometry holds the potential for extensive searches of stellar variability, and provide solid candidates for the upcoming TESS mission. Previous work by e.g. [15], [5], [12] has shown that the WASP archive provides a wealth of pulsationally variable stars. In this talk I will provide an overview of the SuperWASP project, present some of the published results from the survey, and some of the on-going work to identify key targets for the TESS mission

High-frequencies in TESS A–F main sequence stars

The driving mechanism for high-frequency oscillations in some chemically peculiar Ap stars, the roAp stars, is not understood. The TESS data provide an ideal opportunity to extend the number of roAp stars which might provide further clues to address this problem. From an examination of over 18000 stars in TESS sectors 1–7, we have discovered high-frequency pulsations in 14 A–F stars, of which only 3 are classified as Ap stars. In addition to these new discoveries, we discuss the frequencies in 9 previously known roAp stars. In one of these stars, HD 60435, we confirm a previous finding that the pulsations have lifetimes of only a few days. In another known roAp star, HD 6532, the relative amplitudes of the rotationally-modulated sidelobes, which are generally used to estimate the inclination of the magnetic axis relative to the rotational axis, are significantly different from those found in ground-based B-band photometric observations. We also discuss 4 δ Scuti stars which appear to have independent frequencies similar to those of roAp stars

Producing and Measuring Oscillatory Shear in a Novel Microfluidic Chip

Purpose: To demonstrate the effectiveness of a novel microfluidic device mimicking oscillatory blood flow, allowing cell biologists to examine how endothelial cells respond to a range of oscillatory shear stress levels. Methods: The microfluidic chip consists of a circular-shaped reservoir, leading to a rectangular channel that is examined under a microscope. The plunger is connected to a speaker system and oscilloscope, allowing the plunger to apply a range of frequencies (5-60Hz) and voltages (5-10 V, leading to a variety in oscillation amplitudes) to the reservoir region. 1.1 um fluorescent particles diluted in distilled water were used for tracking. Processing was done through particle image velocimetry (PIV) which uses a cross-correlation algorithm. We used matlab to plot average velocity profiles for a cycle, and extracted data points along the centre of the velocity profiles corresponding to the maximum velocities. Results: The oscillatory chip demonstrated the ability to effectively and accurately deliver oscillatory flow between 10-60Hz using 5-10V, resulting in a variety of oscillation frequencies and amplitudes. Plotting velocity maximum values vs. voltage for frequencies 10-60 Hz demonstrated a linear trend. 3D oscillatory-flow paraboloids can be used in calculating maximum shear stress values for oscillatory flow. Conclusions: Our analysis demonstrates that this microfluidic chip is able to execute controlled shear stress conditions to test how endothelial cells respond to oscillatory shear

Nitric oxide sensing in plants is mediated by proteolytic control of group VII ERF transcription factors

Nitric oxide (NO) is an important signaling compound in prokaryotes and eukaryotes. In plants, NO regulates critical developmental transitions and stress responses. Here, we identify a mechanism for NO sensing that coordinates responses throughout development based on targeted degradation of plant-specific transcriptional regulators, the group VII ethylene response factors (ERFs). We show that the N-end rule pathway of targeted proteolysis targets these proteins for destruction in the presence of NO, and we establish them as critical regulators of diverse NO-regulated processes, including seed germination, stomatal closure, and hypocotyl elongation. Furthermore, we define the molecular mechanism for NO control of germination and crosstalk with abscisic acid (ABA) signaling through ERF-regulated expression of ABSCISIC ACID INSENSITIVE5 (ABI5). Our work demonstrates how NO sensing is integrated across multiple physiological processes by direct modulation of transcription factor stability and identifies group VII ERFs as central hubs for the perception of gaseous signals in plants

The effects of dietary fish oil on exercising skeletal muscle vascular and metabolic control in chronic heart failure rats

The ATP-sensitive K+ (KATP) channel is a class of inward rectifier K+ channels that can link cellular metabolic status to vasomotor tone across the metabolic transients seen with exercise. This investigation tested the hypothesis that if KATP channels are crucial to exercise hyperaemia then blockade via glibenclamide (GLI) would lower hindlimb skeletal muscle blood flow (BF) and vascular conductance (VC) during treadmill exercise. In 14 adult male Sprague Dawley rats mean arterial pressure (MAP), blood [lactate], and hindlimb muscle BF (radiolabelled microspheres) were determined at rest (n = 6) or during exercise (n = 8; 20 m min⁻¹, 5% incline) under control (CON) and GLI conditions (5 mg kg⁻¹, i.a). At rest and during exercise, MAP was higher (Rest, CON: 130 ± 6, GLI: 152 ± 8; Exercise, CON: 140 ± 4, GLI: 147 ± 4 mmHg, P < 0.05) and heart rate (HR) was lower (Rest, CON: 440 ± 16, GLI: 410 ± 18; Exercise, CON: 560 ± 4, GLI: 540 ± 10 beats min⁻¹, P < 0.05) with GLI. Hindlimb muscle BF (CON: 144 ± 10, GLI: 120 ± 9 ml min⁻¹ (100 g)⁻¹, P < 0.05) and VC were lower with GLI during exercise but not at rest. Specifically, GLI decreased BF in 12, and VC in 16, of the 28 individual hindlimb muscles and muscle parts sampled during exercise with a greater fractional reduction present in muscles comprised predominantly of type I and type IIa fibres (P < 0.05). Additionally, blood [lactate] (CON: 2.0 ± 0.3; GLI: 4.1 ± 0.9 mmol L⁻¹, P < 0.05) was higher during exercise with GLI. That KATP channel blockade reduces hindlimb muscle BF during exercise in rats supports the obligatory contribution of KATP channels in large muscle mass exercise-induced hyperaemia

Ethylene-mediated nitric oxide depletion pre-adapts plants to hypoxia stress

Timely perception of adverse environmental changes is critical for survival. Dynamic changes in gases are important cues for plants to sense environmental perturbations, such as submergence. In Arabidopsis thaliana, changes in oxygen and nitric oxide (NO) control the stability of ERFVII transcription factors. ERFVII proteolysis is regulated by the N-degron pathway and mediates adaptation to flooding-induced hypoxia. However, how plants detect and transduce early submergence signals remains elusive. Here we show that plants can rapidly detect submergence through passive ethylene entrapment and use this signal to pre-adapt to impending hypoxia. Ethylene can enhance ERFVII stability prior to hypoxia by increasing the NO-scavenger PHYTOGLOBIN1. This ethylene-mediated NO depletion and consequent ERFVII accumulation pre-adapts plants to survive subsequent hypoxia. Our results reveal the biological link between three gaseous signals for the regulation of flooding survival and identifies key regulatory targets for early stress perception that could be pivotal for developing flood-tolerant crops

HD 24355 observed by the Kepler K2 mission: a rapidly oscillating Ap star pulsating in a distorted quadrupole mode

We present an analysis of the first Kepler K2 mission observations of a rapidly oscillating Ap (roAp) star, HD 24355 (V = 9.65). The star was discovered in SuperWASP broad-band photometry with a frequency of 224.31 d−1 (2596.18 μHz; P = 6.4 min) and an amplitude of 1.51 mmag, with later spectroscopic analysis of low-resolution spectra showing HD 24355 to be an A5 Vp SrEu star. The high-precision K2 data allow us to identify 13 rotationally split sidelobes to the main pulsation frequency of HD 24355. This number of sidelobes combined with an unusual rotational phase variation show this star to be the most distorted quadrupole roAp pulsator yet observed. In modelling this star, we are able to reproduce well the amplitude modulation of the pulsation, and find a close match to the unusual phase variations. We show this star to have a pulsation frequency higher than the critical cut-off frequency. This is currently the only roAp star observed with the Kepler spacecraft in short cadence mode that has a photometric amplitude detectable from the ground, thus allowing comparison between the mmag amplitude ground-based targets and the μmag spaced-based discoveries. No further pulsation modes are identified in the K2 data, showing this star to be a single-mode pulsator

The GW Vir instability strip in the light of new observations of PG 1159 stars. Discovery of pulsations in the central star of Abell 72 and variability of RX J0122.9-7521

We present the results of new time series photometric observations of 29 pre-white dwarf stars of PG 1159 spectral type, carried out in the years 2014-2022. For the majority of stars, a median noise level in Fourier amplitude spectra of 0.5-1.0 mmag was achieved. This allowed the detection of pulsations in the central star of planetary nebula Abell 72, consistent with g-modes excited in GW Vir stars, and variability in RX J0122.9-7521 that could be due to pulsations, binarity or rotation. For the remaining stars from the sample that were not observed to vary, we placed upper limits for variability. After combination with literature data, our results place the fraction of pulsating PG 1159 stars within the GW Vir instability strip at 36%. An updated list of all known PG 1159 stars is provided, containing astrometric measurements from the recent Gaia DR3 data, as well as information on physical parameters, variability, and nitrogen content. Those data are used to calculate luminosities for all PG 1159 stars to place the whole sample on the theoretical Hertzsprung-Russell diagram for the first time in that way. The pulsating stars are discussed as a group, and arguments are given that the traditional separation of GW Vir pulsators in "DOV" and "PNNV" stars is misleading and should not be used.Comment: Accepted for publication in ApJ