High potential for loss of permafrost landforms in a changing climate

The presence of ground ice in Arctic soils exerts a major effect on permafrost hydrology and ecology, and factors prominently into geomorphic landform development. As most ground ice has accumulated in near-surface permafrost, it is sensitive to variations in atmospheric conditions. Typical and regionally widespread permafrost landforms such as pingos, ice-wedge polygons, and rock glaciers are closely tied to ground ice. However, under ongoing climate change, suitable environmental spaces for preserving landforms associated with ice-rich permafrost may be rapidly disappearing. We deploy a statistical ensemble approach to model, for the first time, the current and potential future environmental conditions of three typical permafrost landforms, pingos, ice-wedge polygons and rock glaciers across the Northern Hemisphere. We show that by midcentury, the landforms are projected to lose more than one-fifth of their suitable environments under a moderate climate scenario (RCP4.5) and on average around one-third under a very high baseline emission scenario (RCP8.5), even when projected new suitable areas for occurrence are considered. By 2061-2080, on average more than 50% of the recent suitable conditions can be lost (RCP8.5). In the case of pingos and ice-wedge polygons, geographical changes are mainly attributed to alterations in thawing-season precipitation and air temperatures. Rock glaciers show air temperature-induced regional changes in suitable conditions strongly constrained by topography and soil properties. The predicted losses could have important implications for Arctic hydrology, geo- and biodiversity, and to the global climate system through changes in biogeochemical cycles governed by the geomorphology of permafrost landscapes. Moreover, our projections provide insights into the circumpolar distribution of various ground ice types and help inventory permafrost landforms in unmapped regions.Peer reviewe

Life history correlates of faecal bacterial species richness in a wild population of the blue tit Cyanistes caeruleus

Very little is known about the normal gastrointestinal flora of wild birds, or how it might affect or reflect the host's life-history traits. The aim of this study was to survey the species richness of bacteria in the feces of a wild population of blue tits Cyanistes caeruleus and to explore the relationships between bacterial species richness and various life-history traits, such as age, sex, and reproductive success. Using PCR-TGGE, 55 operational taxonomic units (OTUs) were identified in blue tit feces. DNA sequencing revealed that the 16S rRNA gene was amplified from a diverse range of bacteria, including those that shared closest homology with Bacillus licheniformis, Campylobacter lari, Pseudomonas spp., and Salmonella spp. For adults, there was a significant negative relationship between bacterial species richness and the likelihood of being detected alive the following breeding season; bacterial richness was consistent across years but declined through the breeding season; and breeding pairs had significantly more similar bacterial richness than expected by chance alone. Reduced adult survival was correlated with the presence of an OTU most closely resembling C. lari; enhanced adult survival was associated with an OTU most similar to Arthrobacter spp. For nestlings, there was no significant change in bacterial species richness between the first and second week after hatching, and nestlings sharing the same nest had significantly more similar bacterial richness. Collectively, these results provide compelling evidence that bacterial species richness was associated with several aspects of the life history of their hosts

AglH, a thermophilic UDP‑<i>N</i>‑acetylglucosamine‑1‑phosphate:dolichyl phosphate GlcNAc‑1‑phosphotransferase initiating protein<i> N</i>‑glycosylation pathway in <i>Sulfolobus acidocaldarius</i>, is capable of complementing the eukaryal Alg7

AglH, a predicted UDP-GlcNAc-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase, is initiating the protein N-glycosylation pathway in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius. AglH successfully replaced the endogenous GlcNAc-1-phosphotransferase activity of Alg7 in a conditional lethal Saccharomyces cerevisiae strain, in which the first step of the eukaryal protein N-glycosylation process was repressed. This study is one of the few examples of cross-domain complementation demonstrating a conserved polyprenyl phosphate transferase reaction within the eukaryal and archaeal domain like it was demonstrated for Methanococcus voltae (Shams-Eldin et al. 2008). The topology prediction and the alignment of the AglH membrane protein with GlcNAc-1-phosphotransferases from the three domains of life show significant conservation of amino acids within the different proposed cytoplasmic loops. Alanine mutations of selected conserved amino acids in the putative cytoplasmic loops II (D(100)), IV (F(220)) and V (F(264)) demonstrated the importance of these amino acids for cross-domain AlgH activity in in vitro complementation assays in S. cerevisiae. Furthermore, antibiotic treatment interfering directly with the activity of dolichyl phosphate GlcNAc-1-phosphotransferases confirmed the essentiality of N-glycosylation for cell survival