Conditioning of Velocity Profiles in Pipelines

This paper reports on the testing of a relatively simple flow conditioner for pipelines. This research found that a pipeline velocity flow conditioner that is constructed with an internal cone, having an inside diameter of 80 percent of the pipeline ID, provided the best results. It provided good velocity conditioning at a distance of two diameters downstream of an obstruction. The minor loss is equal to the velocity head of the flow in the original pipeline diameter

Measuring Perceived Realistic Physical Threat Imposed by Migrants: Scale Development and Validation

Individuals differ in the extent to which they perceive threat imposed by out-groups like migrants. An established distinction in intergroup threat research is between symbolic and realistic threat. While symbolic threats concern a perceived menace against societal values, realistic threats jeopardize in-group members’ well-being more directly. Typically applied realistic threat conceptions explicitly include the aspect of physical integrity, but most empirical research captures only realistic economic threats, arguably also due to a lack of appropriate measures. Therefore, we have developed the Perceived Realistic Physical Threat scale (PRPT) with samples from Germany and the UK (total N = 1,391). Moreover, we conducted follow-up analyses with data from a subsample (N = 473) of the initial UK sample. Factor analyses indicated an 8-item one-factorial solution for the PRPT scale. We further identified measurement invariance across samples and over time and stability across 21 months. We found convincing evidence for its convergent and divergent validity and for its predictive and, importantly, incremental validity, above and beyond the prediction of relevant criteria by other threat types. The PRPT scale appears to be a distinct, comprehensive, and psychometrically sound measure of perceived realistic physical threat, complementing the existing body of available measures

Ugandan and British individuals' views of refugees in their countries: An exploratory <scp>mixed‐methods</scp> comparison

Abstract: Using an exploratory mixed‐methods approach, we examined thoughts concerning refugees reported by participants from a non‐Western country, Uganda, and the United Kingdom (total N = 113). We explored whether, due to various sociocultural, political and geographic differences, critical features of refugee migration (e.g., migration forcedness and migration‐related perils) would be viewed differently by Ugandan and UK participants. An inductive qualitative content analysis of responses in an online survey yielded 11 categories with 40 subcategories revealing several similarities between Ugandan and UK participants. For instance, similar proportions of participants from both countries acknowledged refugees' suffering before their migration and the forced nature of refugees' migration. However, we also found that more British than Ugandan participants referred to perils refugees suffer during their journeys, possibly resulting from differences in refugees' migration routes (e.g., crossing other countries, travelling by dilapidated boats, migration duration). Furthermore, Ugandan but not British participants took pride in international praise their country received for its forthcoming treatment of refugees. There were no differences regarding the extent to which Ugandan and British individuals exhibited prejudice towards refugees or experienced threats from refugees. We discuss the theoretical and practical implications of our findings for refugee integration

Ab-Initio Molecular Dynamics

Computer simulation methods, such as Monte Carlo or Molecular Dynamics, are very powerful computational techniques that provide detailed and essentially exact information on classical many-body problems. With the advent of ab-initio molecular dynamics, where the forces are computed on-the-fly by accurate electronic structure calculations, the scope of either method has been greatly extended. This new approach, which unifies Newton's and Schr\"odinger's equations, allows for complex simulations without relying on any adjustable parameter. This review is intended to outline the basic principles as well as a survey of the field. Beginning with the derivation of Born-Oppenheimer molecular dynamics, the Car-Parrinello method and the recently devised efficient and accurate Car-Parrinello-like approach to Born-Oppenheimer molecular dynamics, which unifies best of both schemes are discussed. The predictive power of this novel second-generation Car-Parrinello approach is demonstrated by a series of applications ranging from liquid metals, to semiconductors and water. This development allows for ab-initio molecular dynamics simulations on much larger length and time scales than previously thought feasible.Comment: 13 pages, 3 figure

Circulating adrenomedullin estimates survival and reversibility of organ failure in sepsis: the prospective observational multinational Adrenomedullin and Outcome in Sepsis and Septic Shock-1 (AdrenOSS-1) study

Background: Adrenomedullin (ADM) regulates vascular tone and endothelial permeability during sepsis. Levels of circulating biologically active ADM (bio-ADM) show an inverse relationship with blood pressure and a direct relationship with vasopressor requirement. In the present prospective observational multinational Adrenomedullin and Outcome in Sepsis and Septic Shock 1 (, AdrenOSS-1) study, we assessed relationships between circulating bio-ADM during the initial intensive care unit (ICU) stay and short-term outcome in order to eventually design a biomarker-guided randomized controlled trial. Methods: AdrenOSS-1 was a prospective observational multinational study. The primary outcome was 28-day mortality. Secondary outcomes included organ failure as defined by Sequential Organ Failure Assessment (SOFA) score, organ support with focus on vasopressor/inotropic use, and need for renal replacement therapy. AdrenOSS-1 included 583 patients admitted to the ICU with sepsis or septic shock. Results: Circulating bio-ADM levels were measured upon admission and at day 2. Median bio-ADM concentration upon admission was 80.5 pg/ml [IQR 41.5-148.1 pg/ml]. Initial SOFA score was 7 [IQR 5-10], and 28-day mortality was 22%. We found marked associations between bio-ADM upon admission and 28-day mortality (unadjusted standardized HR 2.3 [CI 1.9-2.9]; adjusted HR 1.6 [CI 1.1-2.5]) and between bio-ADM levels and SOFA score (p &lt; 0.0001). Need of vasopressor/inotrope, renal replacement therapy, and positive fluid balance were more prevalent in patients with a bio-ADM &gt; 70 pg/ml upon admission than in those with bio-ADM ≤ 70 pg/ml. In patients with bio-ADM &gt; 70 pg/ml upon admission, decrease in bio-ADM below 70 pg/ml at day 2 was associated with recovery of organ function at day 7 and better 28-day outcome (9.5% mortality). By contrast, persistently elevated bio-ADM at day 2 was associated with prolonged organ dysfunction and high 28-day mortality (38.1% mortality, HR 4.9, 95% CI 2.5-9.8). Conclusions: AdrenOSS-1 shows that early levels and rapid changes in bio-ADM estimate short-term outcome in sepsis and septic shock. These data are the backbone of the design of the biomarker-guided AdrenOSS-2 trial. Trial registration: ClinicalTrials.gov, NCT02393781. Registered on March 19, 2015

Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy

This work was supported by the Italian Ministry of Education, University and Research, “Futuro in Ricerca 2013” program RBFR1334SB to CP

Primate TNF Promoters Reveal Markers of Phylogeny and Evolution of Innate Immunity

Background. Tumor necrosis factor (TNF) is a critical cytokine in the immune response whose transcriptional activation is controlled by a proximal promoter region that is highly conserved in mammals and, in particular, primates. Specific single nucleotide polymorphisms (SNPs) upstream of the proximal human TNF promoter have been identified, which are markers of human ancestry. Methodology/Principal findings. Using a comparative genomics approach we show that certain fixed genetic differences in the TNF promoter serve as markers of primate speciation. We also demonstrate that distinct alleles of most human TNF promoter SNPs are identical to fixed nucleotides in primate TNF promoters. Furthermore, we identify fixed genetic differences within the proximal TNF promoters of Asian apes that do not occur in African ape or human TNF promoters. Strikingly, protein-DNA binding assays and gene reporter assays comparing these Asian ape TNF promoters to African ape and human TNF promoters demonstrate that, unlike the fixed differences that we define that are associated with primate phylogeny, these Asian ape-specific fixed differences impair transcription factor binding at an Sp1 site and decrease TNF transcription induced by bacterial stimulation of macrophages. Conclusions/significance. Here, we have presented the broadest interspecies comparison of a regulatory region of an innate immune response gene to date. We have characterized nucleotide positions in Asian ape TNF promoters that underlie functional changes in cell type- and stimulus-specific activation of the TNF gene. We have also identified ancestral TNF promoter nucleotide states in the primate lineage that correspond to human SNP alleles. These findings may reflect evolution of Asian and African apes under a distinct set of infectious disease pressures involving the innate immune response and TNF

Multi-Platform Next-Generation Sequencing of the Domestic Turkey (Meleagris gallopavo): Genome Assembly and Analysis

The combined application of next-generation sequencing platforms has provided an economical approach to unlocking the potential of the turkey genome