Stereospecific hydroxylation of long chain compounds by a species of Torulopsis.

Abstract A species of yeast of the genus Torulopsis hydroxylates long chain C18 compounds and then converts them to glycosides of 17-l-hydroxy C18 fatty acids. Incubation of methyl [17-18O]hydroxyoleate with whole cells and of methyl oleate in the presence of 18O2 or H218O showed that the oxygen atom, introduced on hydroxylation, is not lost on glycoside formation and that it is derived from molecular oxygen and not from water. Esters of [18-2H3], [16,18-2H5], [17-2H2], [17-d-2H], and [17-l-2H]octadecanoates have been synthesized. On incubation of these compounds no deuterium atoms at C-16 and C-18 are removed but the 17-l-deuterium atom is lost. Unsaturated intermediates are, therefore, most probably not involved and 17-l-hydroxy acid is produced by displacement of an l-hydrogen atom (retention of configuration). The rate of formation of glycoside from l-deuterostearate was less than half of that from d-deuterostearate or from unlabeled stearate, suggesting the operation of a primary isotope effect

The MeV spectral tail in Cyg X-1 and optically-thin emission of jets

We study the average X-ray and soft gamma-ray spectrum of Cyg X-1 in the hard spectral state, using data from INTEGRAL. We compare these results with those from CGRO, and find a good agreement. Confirming previous studies, we find the presence of a high-energy MeV tail beyond a thermal-Comptonization spectrum; however, the tail is much softer and weaker than that recently published by Laurent et al. In spite of this difference, the observed high-energy tail could still be due to the synchrotron emission of the jet of Cyg X-1, as claimed by Laurent et al. To test this possibility, we study optically-thin synchrotron and self-Compton emission from partially self-absorbed jets. We develop formalisms for calculating both emission of the jet base (which we define here as the region where the jet starts its emission) and emission of the entire jet. We require the emission to match that observed at the turnover energy. The optically thin emission is dominated by that from the jet base, and it has to become self-absorbed within it at the turnover frequency. We find this implies the magnetic field strength at the jet base of B_0 prop. to z_0^4, where z_0 is the distance of the base from the black-hole centre. The value of B_0 is then constrained from below by the condition that the self-Compton emission is below an upper limit in the GeV range, and from above by the condition that the Poynting flux does not exceed the jet kinetic power. This yields B_0 of the order of ~10^4 G and the location of the jet base at ~10^3 gravitational radii. Using our formalism, we find the MeV tail can be due to jet synchrotron emission, but this requires the electron acceleration at a rather hard power-law index, p~1.3-1.6. For acceleration indices of p> 2, the amplitude of the synchrotron component is much below that of MeV tail, and its origin is likely to be due to hybrid Comptonization in the accretion flow.Comment: MNRAS, in press, 13 page

A broadband leptonic model for gamma-ray emitting microquasars

Observational and theoretical studies point to microquasars (MQs) as possible counterparts of a significant fraction of the unidentified gamma-ray sources detected so far. At present, a proper scenario to explain the emission beyond soft X-rays from these objects is not known, nor what the precise connection is between the radio and the high-energy radiation. We develop a new model where the MQ jet is dynamically dominated by cold protons and radiatively dominated by relativistic leptons. The matter content and power of the jet are both related with the accretion process. The magnetic field is assumed to be close to equipartition, although it is attached to and dominated by the jet matter. For the relativistic particles in the jet, their maximum energy depends on both the acceleration efficiency and the energy losses. The model takes into account the interaction of the relativistic jet particles with the magnetic field and all the photon and matter fields. Such interaction produces significant amounts of radiation from radio to very high energies through synchrotron, relativistic Bremsstrahlung, and inverse Compton (IC) processes. Variability of the emission produced by changes in the accretion process (e.g. via orbital eccentricity) is also expected. The effects of the gamma-ray absorption by the external photon fields on the gamma-ray spectrum have been taken into account, revealing clear spectral features that might be observed. This model is consistent to the accretion scenario, energy conservation laws, and current observational knowledge, and can provide deeper physical information of the source when tested against multiwavelength data.Comment: 15 pages, 12 figures, A&A, in press (text and plots improved after minor corrections in calculations, text improved also by referee comments

Theoretical overview on high-energy emission in microquasars

Microquasar (MQ) jets are sites of particle acceleration and synchrotron emission. Such synchrotron radiation has been detected coming from jet regions of different spatial scales, which for the instruments at work nowadays appear as compact radio cores, slightly resolved radio jets, or (very) extended structures. Because of the presence of relativistic particles and dense photon, magnetic and matter fields, these outflows are also the best candidates to generate the very high-energy (VHE) gamma-rays detected coming from two of these objects, LS 5039 and LS I +61 303, and may be contributing significantly to the X-rays emitted from the MQ core. In addition, beside electromagnetic radiation, jets at different scales are producing some amount of leptonic and hadronic cosmic rays (CR), and evidences of neutrino production in these objects may be eventually found. In this work, we review on the different physical processes that may be at work in or related to MQ jets. The jet regions capable to produce significant amounts of emission at different wavelengths have been reduced to the jet base, the jet at scales of the order of the size of the system orbital semi-major axis, the jet middle scales (the resolved radio jets), and the jet termination point. The surroundings of the jet could be sites of multiwavelegnth emission as well, deserving also an insight. We focus on those scenarios, either hadronic or leptonic, in which it seems more plausible to generate both photons from radio to VHE and high-energy neutrinos. We briefly comment as well on the relevance of MQ as possible contributors to the galactic CR in the GeV-PeV range.Comment: Astrophysics & Space Science, in press (invited talk in the conference: The multimessenger approach to the high-energy gamma-ray sources", Barcelona/Catalonia, in July 4-7); 10 pages, 6 figures, 2 tables (one reference corrected

Long term variability of Cygnus X-1, III. Radio-X-ray correlations

Long time scale radio-X-ray correlations in black holes during the hard state have been found in many sources and there seems to emerge a universal underlying relationship which quantitatively describes this behavior. Although it would appear only natural to detect short term emission patterns in the X-ray and - with a certain time lag - in the radio, there has been little evidence for this up to now. The most prominent source for radio-X-ray correlations on short time scales (minutes) so far remains GRS 1915+105 where a single mass ejection could be detected successively in the X-ray, IR, and radio wavebands. We analyze a database of more than 4 years of simultaneous radio-X-ray data for Cygnus X-1 from the Ryle Telescope and RXTE PCA/HEXTE. We confirm the existence of a radio-X-ray correlation on long time scales, especially at hard energies. We show that apparent correlations on short time scales in the lightcurves of Cygnus X-1 are most likely the coincidental outcome of white noise statistics. Interpreting this result as a breakdown of radio-X-ray correlations on shorter time scales, this sets a limit to the speed of the jet.Comment: 8 pages, 7 figures, accepted for publication in A&

A decade of radio imaging the relativistic outflow in the peculiar X-ray binary Circinus X-1

We present observations of the neutron star X-ray binary and relativistic jet source Circinus X-1 made at 4.8 and 8.6 GHz with the Australia Telescope Compact Array during a time interval of almost 10 years. The system shows significant variations in the morphology and brightness of the radio features on all timescales from days to years. Using the time delay between the successive brightening of the different components of the radio emission we were able to provide further evidence for the relativistic nature of the arcsec scale outflow, with an apparent velocity beta_app >= 12. No compelling evidence for an evolution of the orientation of the jet axis was found. We also place an upper limit on the proper motion of the system which is consistent with previous optical studies. Besides the previously reported radio flares close to the orbital phase 0.0 (interpreted as enhanced accretion at periastron passage), we also identified outbursts with similar properties near the orbital phase 0.5. The global spectral index revealed a preferentially steep spectrum over the entire period of monitoring with a mean value and standard deviation alpha=-0.9 +/- 0.6 (F_nu ~ nu^{alpha}), which became significantly flatter during the outbursts. Polarization was detected in one third of the epochs and in one case Faraday rotation close to the core of the system was measured.Comment: Accepted for publication in MNRA

Overview of the perceived risk of transboundary pig diseases in South Africa

Pig production is one of the most important animal agricultural activities in South Africa, and plays a definite role in providing food security for certain population groups in the country. As with all animal production systems, it is subject to the risk of outbreak of transboundary diseases. In the present overview, evaluations of the perceived risk of selected transboundary animal diseases of pigs, as collated from the willing participants from the provincial veterinary services of South Africa, are presented. A scenario tree revealed that infected but undetected pigs were the greatest perceived threat. The provincial veterinary services, according to participants in the study, face certain difficulties, including the reporting of disease and the flow of disease information amongst farmers. Perceived strengths in surveillance and disease monitoring include the swiftness of sample despatch to the national testing laboratory, as well as the ease of flow of information between the provincial and national agricultural authorities. The four factors were identified that were perceived to most influence animal health-service delivery: transport, access, livestock policy and resources. African swine fever was perceived to be the most important pig disease in South Africa. Because the decentralisation of veterinary services in South Africa was identified as a potential weakness, it is recommended that national and provincial veterinary services need to work together and interdependently to achieve centrally controlled surveillance systems. Regionally-coordinated surveillance activities for certain transboundary diseases were identified as needing priority for the southern African region. It is proposed that an emergency preparedness document be made available and regularly revised according to the potential risks identified on a continuous basis for South Africa.http://www.jsava.co.zaam201

Overview of the perceived risk of transboundary pig diseases in South Africa

Pig production is one of the most important animal agricultural activities in South Africa, and plays a definite role in providing food security for certain population groups in the country. As with all animal production systems, it is subject to the risk of outbreak of transboundary diseases. In the present overview, evaluations of the perceived risk of selected transboundary animal diseases of pigs, as collated from the willing participants from the provincial veterinary services of South Africa, are presented. A scenario tree revealed that infected but undetected pigs were the greatest perceived threat. The provincial veterinary services, according to participants in the study, face certain difficulties, including the reporting of disease and the flow of disease information amongst farmers. Perceived strengths in surveillance and disease monitoring include the swiftness of sample despatch to the national testing laboratory, as well as the ease of flow of information between the provincial and national agricultural authorities. The four factors were identified that were perceived to most influence animal health-service delivery: transport, access, livestock policy and resources. African swine fever was perceived to be the most important pig disease in South Africa. Because the decentralisation of veterinary services in South Africa was identified as a potential weakness, it is recommended that national and provincial veterinary services need to work together and interdependently to achieve centrally controlled surveillance systems. Regionally-coordinated surveillance activities for certain transboundary diseases were identified as needing priority for the southern African region. It is proposed that an emergency preparedness document be made available and regularly revised according to the potential risks identified on a continuous basis for South Africa.http://www.jsava.co.zaam201

Pelargonidin-3-O-glucoside and its metabolites have modest anti-inflammatory effects in human whole blood cultures

This study hypothesized that the predominant strawberry anthocyanin, pelargonidin-3-O-glucoside (Pg-3-glc), and three of its plasma metabolites (4-hydroxybenzoic acid, protocatechuic acid and phloroglucinaldehyde [PGA]) would affect phagocytosis, oxidative burst and the production of selected pro- and anti-inflammatory cytokines in a whole blood culture model. For the assessment of phagocytosis and oxidative burst activity of monocytes and neutrophils, whole blood was pre-incubated in the presence or absence of the test compounds at concentrations up to 5 μM, followed by analysis of phagocytic and oxidative burst activity using commercially available test kits. For the cytokine analysis, diluted whole blood was stimulated with lipopolysaccharide in the presence or absence of the test compounds at concentrations up to 5 μM. Concentrations of selected cytokines (tumor necrosis factor-α [TNF-α], interleukin [IL]-1β, IL-6, IL-8 and IL-10) were determined using a cytometric bead array kit. There were no effects of any of the test compounds on phagocytosis of opsonized or non-opsonized E. coli or on oxidative burst activity. Pg-3-glc and PGA at 0.08 μM increased the concentration of IL-10 (P<0.01 and P<0.001, respectively), but there was no effect on TNF-α, IL-1β, IL-6 and IL-8 and there were no effects of the other compounds. In conclusion, this study demonstrated a lack of effect of these compounds on the opsonization, engulfment and subsequent destruction of bacteria. Pg-3-glc and PGA, at physiologically relevant concentrations, had anti-inflammatory properties; however, effects were modest, only observed at the lowest dose tested and limited to IL-10