132 research outputs found
Genetic drift at expanding frontiers promotes gene segregation
Competition between random genetic drift and natural selection plays a
central role in evolution: Whereas non-beneficial mutations often prevail in
small populations by chance, mutations that sweep through large populations
typically confer a selective advantage. Here, however, we observe chance
effects during range expansions that dramatically alter the gene pool even in
large microbial populations. Initially well-mixed populations of two
fluorescently labeled strains of Escherichia coli develop well-defined,
sector-like regions with fractal boundaries in expanding colonies. The
formation of these regions is driven by random fluctuations that originate in a
thin band of pioneers at the expanding frontier. A comparison of bacterial and
yeast colonies (Saccharomyces cerevisiae) suggests that this large-scale
genetic sectoring is a generic phenomenon that may provide a detectable
footprint of past range expansions.Comment: Please visit http://www.pnas.org/content/104/50/19926.abstract for
published articl
Improved Network Performance via Antagonism: From Synthetic Rescues to Multi-drug Combinations
Recent research shows that a faulty or sub-optimally operating metabolic
network can often be rescued by the targeted removal of enzyme-coding
genes--the exact opposite of what traditional gene therapy would suggest.
Predictions go as far as to assert that certain gene knockouts can restore the
growth of otherwise nonviable gene-deficient cells. Many questions follow from
this discovery: What are the underlying mechanisms? How generalizable is this
effect? What are the potential applications? Here, I will approach these
questions from the perspective of compensatory perturbations on networks.
Relations will be drawn between such synthetic rescues and naturally occurring
cascades of reaction inactivation, as well as their analogues in physical and
other biological networks. I will specially discuss how rescue interactions can
lead to the rational design of antagonistic drug combinations that select
against resistance and how they can illuminate medical research on cancer,
antibiotics, and metabolic diseases.Comment: Online Open "Problems and Paradigms" articl
Analysis of genetic systems using experimental evolution and whole-genome sequencing
The application of whole-genome sequencing to the study of microbial evolution promises to reveal the complex functional networks of mutations that underlie adaptation. A recent study of parallel evolution in populations of Escherichia coli shows how adaptation involves both functional changes to specific proteins as well as global changes in regulation
Recombination Speeds Adaptation by Reducing Competition between Beneficial Mutations in Populations of Escherichia coli
Identification of the selective forces contributing to the origin and maintenance of sex is a fundamental problem in biology. The Fisher–Muller model proposes that sex is advantageous because it allows beneficial mutations that arise in different lineages to recombine, thereby reducing clonal interference and speeding adaptation. I used the F plasmid to mediate recombination in the bacterium Escherichia coli and measured its effect on adaptation at high and low mutation rates. Recombination increased the rate of adaptation ∼3-fold more in the high mutation rate treatment, where beneficial mutations had to compete for fixation. Sequencing of candidate loci revealed the presence of a beneficial mutation in six high mutation rate lines. In the absence of recombination, this mutation took longer to fix and, over the course of its substitution, conferred a reduced competitive advantage, indicating interference between competing beneficial mutations. Together, these results provide experimental support for the Fisher–Muller model and demonstrate that plasmid-mediated gene transfer can accelerate bacterial adaptation
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Potentiating antibacterial activity by predictably enhancing endogenous microbial ROS production
The ever-increasing incidence of antibiotic-resistant infections combined with a weak pipeline of new antibiotics has created a global public health crisis1. Accordingly, novel strategies for enhancing our antibiotic arsenal are needed. As antibiotics kill bacteria in part by inducing reactive oxygen species (ROS)2–4, we reasoned that targeting microbial ROS production might potentiate antibiotic activity. Here we show that ROS production can be predictably enhanced in Escherichia coli, increasing the bacteria’s susceptibility to oxidative attack. We developed an ensemble, genome-scale metabolic modeling approach capable of predicting ROS production in E. coli. The metabolic network was systematically perturbed and its flux distribution analyzed to identify targets predicted to increase ROS production. In silico–predicted targets were experimentally validated and shown to confer increased susceptibility to oxidants. Validated targets also increased susceptibility to killing by antibiotics. This work establishes a systems-based method to tune ROS production in bacteria and demonstrates that increased microbial ROS production can potentiate killing by oxidants and antibiotics
Cost of Antibiotic Resistance and the Geometry of Adaptation
The distribution of effects of beneficial mutations is key to our understanding of biological adaptation. Yet, empirical estimates of this distribution are scarce, and its functional form is largely unknown. Theoretical models of adaptation predict that the functional form of this distribution should depend on the distance to the optimum. Here, we estimate the rate and distribution of adaptive mutations that compensate for the effect of a single deleterious mutation, which causes antibiotic resistance. Using a system with multiple molecular markers, we estimate the distribution of fitness effects of mutations at two distances from the adaptive peak in 60 populations of Escherichia coli. We find that beneficial mutations, which can contribute to compensatory evolution, occur at very high rates, of the order of 10−5 per genome per generation and can be detected within a few tens of generations. They cause an average fitness increase of 2.5% and 3.6%, depending on the cost of resistance, which is expected under Fisher's geometrical model of adaptation. Moreover, we provide the first description of the distribution of beneficial mutations, segregating during the process of compensatory evolution, to antibiotic resistances bearing different costs. Hence, these results have important implications to understanding the spread and maintenance of antibiotic resistance in bacteria
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First steps in experimental cancer evolution
Evolutionary processes play a central role in the development, progression and response to treatment of cancers. The current challenge facing researchers is to harness evolutionary theory to further our understanding of the clinical progression of cancers. Central to this endeavour will be the development of experimental systems and approaches by which theories of cancer evolution can be effectively tested. We argue here that the experimental evolution approach – whereby evolution is observed in real time and which has typically employed microorganisms – can be usefully applied to cancer. This approach allows us to disentangle the ecological causes of natural selection, identify the genetic basis of evolutionary changes and determine their repeatability. Cell cultures used in cancer research share many of the desirable traits that make microorganisms ideal for studying evolution. As such, experimental cancer evolution is feasible and likely to give great insight into the selective pressures driving the evolution of clinically destructive cancer traits. We highlight three areas of evolutionary theory with importance to cancer biology that are amenable to experimental evolution: drug resistance, social evolution and resource competition. Understanding the diversity, persistence and evolution of cancers is vital for treatment and drug development, and an experimental evolution approach could provide strategic directions and focus for future research
Heterogeneous Adaptive Trajectories of Small Populations on Complex Fitness Landscapes
Background Small populations are thought to be adaptively handicapped, not only because they suffer more from deleterious mutations but also because they have limited access to new beneficial mutations, particularly those conferring large benefits. Methodology/Principal Findings Here, we test this widely held conjecture using both simulations and experiments with small and large bacterial populations evolving in either a simple or a complex nutrient environment. Consistent with expectations, we find that small populations are adaptively constrained in the simple environment; however, in the complex environment small populations not only follow more heterogeneous adaptive trajectories, but can also attain higher fitness than the large populations. Large populations are constrained to near deterministic fixation of rare large-benefit mutations. While such determinism speeds adaptation on the smooth adaptive landscape represented by the simple environment, it can limit the ability of large populations from effectively exploring the underlying topography of rugged adaptive landscapes characterized by complex environments. Conclusions Our results show that adaptive constraints often faced by small populations can be circumvented during evolution on rugged adaptive landscapes
The Properties of Adaptive Walks in Evolving Populations of Fungus
A novel method to infer the number and fitness effect of beneficial mutations reveals that the bulk of adaptive evolution is attributable to a few mutations with variable effects on fitness
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