Characterization of metal ion-induced [3H]inositol hexakisphosphate binding to rat cerebellar membranes

The binding of [3H]inositol hexakisphosphate ([3H] InsP6) to rat cerebellar membranes has been characterized with the objective of establishing the role, if any, of a membrane protein receptor. In the presence of EDTA, we have previously identified an InsP6-binding site with a capacity of approximately 20 pmol/mg protein (Hawkins, P. T., Reynolds, D. J. M., Poyner, D. R., and Hanley, M. R. (1990) Biochem. Biophys. Res. Commun. 167, 819-827). However, in the presence of 1 mM Mg2+, the capacity of [3H]InsP6 binding to membranes was increased approximately 9-fold. This enhancing effect of Mg2+ was reversed by addition of 10 microM of several cation chelators, suggesting that the increased binding required trace quantities of other metal cations. This is supported by experiments where it was possible to saturate binding by addition of excess membranes, despite not significantly depleting radioligand, pointing to removal of some other factor. Removal of endogenous cations from the binding assay by pretreatment with chelex resin also prevents the Mg(2+)-induced potentiation. Consideration of the specificity of the chelators able to abolish this potentiation suggested involvement of Fe3+ or Al3+. Both these ions (but not several others) were able to increase [3H]InsP6 binding to chelex-pretreated membranes at concentrations of 1 microM. It is possible to demonstrate synergy between Fe3+ and Mg2+ under these conditions. We propose that [3H]InsP6 may interact with membranes through non-protein recognition possibly via phospholipids, in a manner dependent upon trace metals. The implications of this for InsP6 biology are considered

Depression and anxiety in sterilised women in Iran

Sterilisation is an effective and convenient means of contraception and has become increasingly popular as a birth control technique throughout the world during the past 40 years. However some women who choose sterilisation may suffer a neurotic syndrome, which is manifested in the form of pain, depression and loss of libido.1We undertook a study designed to investigate depression, anxiety and post-operation regret rate in sterilised women referred to health centres in Tabriz, Iran in 2006. The study design was descriptive-analytical. The study participants comprised 300 women in the age range 25–45 years, of whom 150 women were sterilized between 1 and 10 years ago and 150 were a control group of non-sterilised women who used condoms, withdrawal or safe period methods for contraception. The control group was selected by a cluster random sampling method. Fifteen health centres were selected as a cluster from 96 health centres located in Tabriz. Ten women were selected randomly from each health centre using health documents. Women were eligible for inclusion in the study if they were aged between 25 and 45 years at the time of sampling, and if they had no history of psychological disorders and no recent sorrowful events. There were no differences between the two groups as regards the number of children, income or demographic characteristics. The women were contacted by telephone at their last known address and were asked to complete questionnaires. Data collection was done using Zung’s self-rating depression and anxiety scale in addition to questions about post-sterilisation regret. Data were collected from the subjects anonymously and analysed using SPSS (v. 11.5) statistics software. Analysis employed t-test, Chi-square test and descriptive statistics. The comparison of the means for depression in the two groups was not significantly different (p = 0.96), however the mean of anxiety in the case group was remarkably greater than the control group (p = 0.03). Insufficient poststerilisation rest was a significant risk factor for depression and anxiety (p = 0.008 and p = 0.02, respectively). Requesting information about reversal after tubal sterilisation was 2.7% and the post-sterilisation regret rate was 6%, which was significantly related to women’s conflict with their husbands about the decision-making process prior to sterilisation (p<0.001). The study findings as regards psychological disorders of sterilisation suggested that women undergoing sterilisation should ensure that they have a good rest after their operation in order to reduce the extent of psychological disorders. Unlike studies undertaken in other countries,2–4 women’s age, parity, marriage duration and the timing of sterilisation was unrelated to the women’s regret in our study. The earlier the sterilisation is carried out, the longer the woman’s remaining period of fertile life and the greater the chances of changes in her marital status and/or the loss of a child, both circumstances that may lead to a change in the desired family size and expression of regret. In our study, probably one of the reasons why women’s regret did not appear to be significantly related to young age of sterilisation was the infrequency of divorce or remarriage in our study population. Consistent with our study, Jamieson et al. reported that women who had substantial conflict with their husbands or partners prior to sterilisation were more than three times as likely to regret their decision and more than five times more likely to request a reversal than women who did not report such conflict.5 In our study, pre-sterilisation counseling was reported by 29.3% of subjects. With respect to personality and adaptability differences in facing the changes, pre-sterilisation counseling and post-sterilisation follow-up systems have an important role to play in women’s psychological and psychosexual health promotion

Study of Interplanetary Magnetic Field with Ground State Alignment

We demonstrate a new way of studying interplanetary magnetic field -- Ground State Alignment (GSA). Instead of sending thousands of space probes, GSA allows magnetic mapping with any ground telescope facilities equipped with spectropolarimeter. The polarization of spectral lines that are pumped by the anisotropic radiation from the Sun is influenced by the magnetic realignment, which happens for magnetic field (<1G). As a result, the linear polarization becomes an excellent tracer of the embedded magnetic field. The method is illustrated by our synthetic observations of the Jupiter's Io and comet Halley. Polarization at each point was constructed according to the local magnetic field detected by spacecrafts. Both spatial and temporal variations of turbulent magnetic field can be traced with this technique as well. The influence of magnetic field on the polarization of scattered light is discussed in detail. For remote regions like the IBEX ribbons discovered at the boundary of interstellar medium, GSA provides a unique diagnostics of magnetic field.Comment: 11 pages, 19 figures, published in Astrophysics and Space Scienc

Effects of unidirectional flow shear stresses on the formation, fractal microstructure and rigidity of incipient whole blood clots and fibrin gels

Incipient clot formation in whole blood and fibrin gels was studied by the rheometric techniques of controlled stress parallel superposition (CSPS) and small amplitude oscillatory shear (SAOS). The effects of unidirectional shear stress on incipient clot microstructure, formation kinetics and elasticity are reported in terms of the fractal dimension (df ) of the fibrin network, the gel network formation time (TGP ) and the shear elastic modulus, respectively. The results of this first haemorheological application of CSPS reveal the marked sensitivity of incipient clot microstructure to physiologically relevant levels of shear stress, these being an order of magnitude lower than have previously been studied by SAOS. CSPS tests revealed that exposure of forming clots to increasing levels of shear stress produces a corresponding elevation in df , consistent with the formation of tighter, more compact clot microstructures under unidirectional flow. A corresponding increase in shear elasticity was recorded. The scaling relationship established between shear elasticity and df for fibrin clots and whole blood confirms the fibrin network as the dominant microstructural component of the incipient clot in terms of its response to imposed stress. Supplementary studies of fibrin clot formation by rheometry and microscopy revealed the substantial additional network mass required to increase df and provide evidence to support the hypothesis that microstructural changes in blood clotted under unidirectional shear may be attributed to flow enhanced thrombin generation and activation. CSPS also identified a threshold value of unidirectional shear stress above which no incipient clot formation could be detected. CSPS was shown to be a valuable haemorheological tool for the study of the effects of physiological and pathological levels of shear on clot properties

From Feshbach-Resonance Managed Bose-Einstein Condensates to Anisotropic Universes: Some Applications of the Ermakov-Pinney equation with Time-Dependent Nonlinearity

In this work we revisit the topic of two-dimensional Bose-Einstein condensates under the influence of time-dependent magnetic confinement and time-dependent scattering length. A moment approach reduces the examination of moments of the wavefunction (in particular, of its width) to an Ermakov-Pinney (EP) ordinary differential equation (ODE). We use the well-known structure of the solutions of this nonlinear ODE to ``engineer'' trapping and interatomic interaction conditions that lead to condensates dispersing, breathing or even collapsing. The advantage of the approach is that it is fully tractable analytically, in excellent agreement with our numerical observations. As an aside, we also discuss how similar time-dependent EP equations may arise in the description of anisotropic scalar field cosmologies.Comment: 9 pages, 4 figure

Cosmological constraints from galaxy clustering

In this manuscript I review the mathematics and physics that underpins recent work using the clustering of galaxies to derive cosmological model constraints. I start by describing the basic concepts, and gradually move on to some of the complexities involved in analysing galaxy redshift surveys, focusing on the 2dF Galaxy Redshift Survey (2dFGRS) and the Sloan Digital Sky survey (SDSS). Difficulties within such an analysis, particularly dealing with redshift space distortions and galaxy bias are highlighted. I then describe current observations of the CMB fluctuation power spectrum, and consider the importance of measurements of the clustering of galaxies in light of recent experiments. Finally, I provide an example joint analysis of the latest CMB and large-scale structure data, leading to a set of parameter constraints.Comment: 30 pages, 13 figures. Lecture given at Third Aegean Summer School, The invisible universe: Dark matter and Dark energ