590 research outputs found
Strain Modulation of Graphene by Nanoscale Substrate Curvatures: A Molecular View
Spatially nonuniform strain is important for engineering the pseudomagnetic
field and band structure of graphene. Despite the wide interest in strain
engineering, there is still a lack of control on device-compatible strain
patterns due to the limited understanding of the structure-strain relationship.
Here, we study the effect of substrate corrugation and curvature on the strain
profiles of graphene via combined experimental and theoretical studies of a
model system: graphene on closely packed SiO2 nanospheres with different
diameters (20-200 nm). Experimentally, via quantitative Raman analysis, we
observe partial adhesion and wrinkle features and find that smaller nanospheres
induce larger tensile strain in graphene, theoretically, molecular dynamics
simulations confirm the same microscopic structure and size dependence of
strain and reveal that a larger strain is caused by a stronger, inhomogeneous
interaction force between smaller nanospheres and graphene. This
molecular-level understanding of the strain mechanism is important for strain
engineering of graphene and other two-dimensional materials.Comment: Nano Letters (2018
Telomerase reverse transcriptase haploinsufficiency and telomere length in individuals with 5p– syndrome
Telomerase, which maintains the ends of chromosomes, consists of two core components, the telomerase reverse transcriptase (TERT) and the telomerase RNA (TERC). Haploinsufficiency for TERC or TERT leads to progressive telomere shortening and autosomal dominant dyskeratosis congenita (DC). The clinical manifestations of autosomal dominant DC are thought to occur when telomeres become critically short, but the rate of telomere shortening in this condition is unknown. Here, we investigated the consequences of de novo TERT gene deletions in a large cohort of individuals with 5p– syndrome. The study group included 41 individuals in which the chromosome deletion resulted in loss of one copy of the TERT gene at 5p15.33. Telomere length in peripheral blood cells from these individuals, although within the normal range, was on average shorter than in normal controls. The shortening was more significant in older individuals suggesting an accelerated age-dependent shortening. In contrast, individuals with autosomal dominant DC due to an inherited TERC gene deletion had very short telomeres, and the telomeres were equally short regardless of the age. Although some individuals with 5p– syndrome showed clinical features that were reminiscent of autosomal dominant DC, these features did not correlate with telomere length, suggesting that these were not caused by critically short telomeres. We conclude that a TERT gene deletion leads to slightly shorter telomeres within one generation. However, our results suggest that several generations of TERT haploinsufficiency are needed to produce the very short telomeres seen in patients with DC
The Role of Detailed Geomorphic Variability in the Vulnerability Assessment of Potential Oil Spill Events on Mixed Sand and Gravel Beaches: The Cases of Two Adriatic Sites
The role of short- to medium-term geomorphic variation is analyzed in two Italian
mixed sand and gravel beaches to better understand how it could affect vulnerability
assessments of oil spill events. The study sites, Portonovo and Sirolo, are in one
of the most congested areas for oil transportation in the Adriatic Sea (Ancona port).
A “snapshot” situation populated with field data collected in April 2015 is compared to a
“changing” situation built with previous field datasets (topographic surveys and surface
sediment samplings) available for the two beaches. According to the ESI guidelines
established by the National Oceanic and Atmospheric Administration [NOAA], 2002,
both Portonovo and Sirolo can be ranked as ESI 5 or 6A in most of the cases. Sediment
size resulted in the most decisive factor for the ESI assessment. As consequence of
the bimodal direction of storms, the high geomorphic variability on the two sites is
mainly related to storm berms which lead to rapid burial processes on both beaches.
In oil spill circumstances, burial is considered the most alarming factor, especially on
microtidal mixed beaches that develop storm berms so high and close to the shoreline.
A quantification of the maximum potential depth reachable by the oil in the beach
body is therefore needed for the most dynamic beaches; this could be achieved with
repeated field measurements to be performed in the period between two consecutive
ESI updates (5–7 years) and the addition of an appendix in the ESI maps dealing with
the geomorphic characteristics of the beach. The significance of a changing ESI rank is
that the authorities in charge of responding to the oil spill could be improperly prepared
for the conditions that exist at a spill site if the geomorphology has changed from when
it was first given an ESI rank
Recommended from our members
Resilience, apps and reluctant individualism: Technologies of self in the neoliberal academy
This paper is concerned with the deep crisis affecting universities, as large scale institutional and structural transformations produce a psychosocial and somatic catastrophe amongst academics (and other university workers) that manifests in experiences of chronic stress, anxiety, exhaustion, insomnia and spiralling rates of physical and mental illness. Elsewhere these have been discussed as the 'hidden injuries of the neoliberal university' (Gill, 2010), highlighting the ways in which such experiences are simultaneously acknowledged and recognised by university staff, yet silenced and exorcised from formal spaces of the contemporary academy and without 'proper channels' of expression - being the subject of conference coffee breaks but not keynotes, of after seminar drinks but not departmental meetings, committee minutes or Senate or Council documentation. For future historians seeking to understand through such official records something about the texture of experience of current academic life, the archives will offer no insights.However, in the last few years, this paper suggests, such injuries have moved from being almost completely silenced within universities to becoming the subject of a variety of new spaces and services designed with 'academics in crisis' at their heart. These include the rolling out of 'well-being' services within universities of programmes for stress management, mindfulness and resilience, the development of new 'apps' designed for busy or overworked people, and the rapidly expanding blogosphere which has become a key site for 'naming' and sharing such experiences of distress/injury. The paper looks critically at these three sites. It argues that whilst they recognise at least some aspects of the subjective experience of contemporary academic labouring, they remain locked into a profoundly individualist framework that turns away from systemic or collective politics to offer instead a set of individualised tools by which to 'cope' with the strains of the neoliberal academy
Traits of neighbouring plants and space limitation determine intraspecific trait variability in semi-arid shrublands
Understanding how intraspecific trait variability (ITV) responds to both abiotic and biotic constraints is crucial to predict how individuals are assembled in plant communities, and how they will be impacted by ongoing global environmental change.Three key functional traits [plant height, leaf area (LA) and specific leaf area (SLA)] were assessed to quantify the range of ITV of four dominant plant species along a rainfall gradient in semi-arid Mediterranean shrublands. Variance partitioning and confirmatory multilevel path analyses were used to assess the direct and indirect effects of rainfall, space limitation (crowding) and neighbouring plant traits on ITV.The direct effect of the local neighbourhood on the trait values of subordinate individuals was as strong as the effect of rainfall. The indirect effect of rainfall, however, mediated by the effect of the local neighbourhood on the trait values of subordinate individuals, was weak. Rainfall decreased the height and SLA of subordinate individuals, but increased their LA. Neighbouring plant traits were just as strong predictors as crowding in explaining changes in ITV.Synthesis. Our study provides a framework to disentangle the direct effects of abiotic factors and their indirect effects on ITV mediated by the local neighbourhood. Our results highlight that abiotic and biotic constraints are both substantial sources of trait variations at the individual level, and can blur processes underlying changes in ITV. Considering and disentangling combined sources with an individual perspective would help to refine our predictions for community assembly and functional ecology
Substituent effects on the in vitro and in vivo genotoxicity of 4-aminobiphenyl and 4-aminostilbene derivatives
4-Amino-4'-substituted biphenyls and 4-aminostilbenes substituted in the 3' or 4' position were studied for their in vitro and in vivo genotoxicity. The in vitro mutagenicity of the biphenyls with and without S9 activation was established with Salmonella strains TA98 and TA100 and that of the stilbenes with the same strains plus TA98/1,8-DNP6. The in vivo genotoxicity assay with both series of compounds was for chromosomal aberrations in the bone-marrow cells of mice following intraperitoneal administration of the chemicals. Hammett values of substituents, partition coefficients and frontier orbital energies (ELUMO and EHOMO) of the compounds were used for correlations with mutagenicity. The Salmonella mutagenicity in TA98 and TA98/1,8-DNP6 with S9 was correlated to Hammett [sigma]+ values for the 4-aminostilbene substituents, showing a strong trend of increasing mutagenicity with an increase in the electron-withdrawing capability of the substituent. Hydrophobicity of the stilbenes, however, had little effect on their relative mutagenicity. The 4-aminobiphenyls showed a correlation between their mutagenicity and Hammett [sigma]+ values of their 4'-substituents in stain TA98 with S9, although the trend was not as strong as for the stilbenes. But unlike the stilbenes, TA98 mutagenicity of the biphenyls could also be correlated to hydrophobicity, and structure-activity correlations for the biphenyls was substantially improved when both [sigma]+ and hydrophobicity data were included. For strain TA100 with S9, little correlation was found between mutagenicity of the stilbenes and any of, the parameters. However, a limited correlation did exist between the mutagenicity of the biphenyls and their hydrophobicity. There was also limited correlations of the mutagenicity for the stilbenes in TA98 and TA98/1,8-DNP6 with S9 to ELUMO or EHOMO. The in vivo genotoxicity results for the biphenyls and stilbenes could not be correlated to electronic effects as for the in vitro results, nor could they be explained by hydrophobicity. However, it is interesting to note that 3'-substituted 4-aminostilbenes were all substantially more genotoxic in vivo than their corresponding 4'-substituted counterparts. The most genotoxic compound in vivo in either series was 4-aminostilbene which would not have been predicted from the in vitro results.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/31865/1/0000815.pd
Endothelial and Smooth Muscle Cells from Abdominal Aortic Aneurysm Have Increased Oxidative Stress and Telomere Attrition
Background: Abdominal aortic aneurysm (AAA) is a complex multi-factorial disease with life-threatening complications. AAA is typically asymptomatic and its rupture is associated with high mortality rate. Both environmental and genetic risk factors are involved in AAA pathogenesis. Aim of this study was to investigate telomere length (TL) and oxidative DNA damage in paired blood lymphocytes, aortic endothelial cells (EC), vascular smooth muscle cells (VSMC), and epidermal cells from patients with AAA in comparison with matched controls. Methods: TL was assessed using a modification of quantitative (Q)-FISH in combination with immunofluorescence for CD31 or α-smooth muscle actin to detect EC and VSMC, respectively. Oxidative DNA damage was investigated by immunofluorescence staining for 7, 8-dihydro-8-oxo-2′-deoxyguanosine (8-oxo-dG). Results and Conclusions: Telomeres were found to be significantly shortened in EC, VSMC, keratinocytes and blood lymphocytes from AAA patients compared to matched controls. 8-oxo-dG immunoreactivity, indicative of oxidative DNA damage, was detected at higher levels in all of the above cell types from AAA patients compared to matched controls. Increased DNA double strand breaks were detected in AAA patients vs controls by nuclear staining for γ-H2AX histone. There was statistically significant inverse correlation between TL and accumulation of oxidative DNA damage in blood lymphocytes from AAA patients. This study shows for the first time that EC and VSMC from AAA have shortened telomeres and oxidative DNA damage. Similar findings were obtained with circulating lymphocytes and keratinocytes, indicating the systemic nature of the disease. Potential translational implications of these findings are discussed. © 2012 Cafueri et al
- …