Re–Os age for the Lower–Middle Pennsylvanian Boundary and comparison with associated palynoflora

AbstractThe Betsie Shale Member is a relatively thick and continuous unit that serves as a marker bed across the central Appalachian basin, in part because it includes an organic-rich shale unit at its base that is observable in drill logs. Deposited during a marine transgression, the Betsie Shale Member has been correlated to units in both Wales and Germany and has been proposed to mark the boundary between the Lower and Middle Pennsylvanian Series within North America. This investigation assigns a new Re–Os date to the base of the Betsie and examines the palynoflora and maceral composition of the underlying Matewan coal bed in the context of that date. The Matewan coal bed contains abundant lycopsid tree spores along its base with assemblage diversity and inertinite content increasing upsection, as sulfur content and ash yield decrease. Taken together, these palynologic and organic petrographic results suggest a submerged paleomire that transitioned to an exposed peat surface. Notably, separating the lower and upper benches of the Matewan is a parting with very high sulfur content (28wt.%), perhaps representing an early marine pulse prior to the full on transgression responsible for depositing the Betsie. Results from Re–Os geochronology date the base of the Betsie at 323±7.8Ma, consistent with previously determined age constraints as well as the palynoflora assemblage presented herein. The Betsie Shale Member is also highly enriched in Re (ranging from 319.7 to 1213ng/g), with high 187Re/188Os values ranging from 3644 to 5737 likely resultant from varying redox conditions between the pore water and overlying water column during deposition and early condensing of the section

Drosophila PINK1 and parkin loss-of-function mutants display a range of non-motor Parkinson’s disease phenotypes.

Parkinson’s disease (PD) is more commonly associated with its motor symptoms and the related degeneration of dopamine (DA) neurons. However, it is becoming increasingly clear that PD patients also display a wide range of non-motor symptoms, including memory deficits and disruptions of their sleep-wake cycles. These have a large impact on their quality of life, and often precede the onset of motor symptoms, but their etiology is poorly understood. The fruit fly Drosophila has already been successfully used to model PD, and has been used extensively to study relevant non-motor behaviours in other contexts, but little attention has yet been paid to modelling non-motor symptoms of PD in this genetically tractable organism. We examined memory performance and circadian rhythms in flies with loss-of-function mutations in two PD genes: PINK1 and parkin. We found learning and memory abnormalities in both mutant genotypes, as well as a weakening of circadian rhythms that is underpinned by electrophysiological changes in clock neurons. Our study paves the way for further work that may help us understand the mechanisms underlying these neglected aspects of PD, thus identifying new targets for treatments to address these non-motor problems specifically and perhaps even to halt disease progression in its prodromal phase

Late Permian–Early Triassic environmental changes recorded by multi-isotope (Re-Os-N-Hg) data and trace metal distribution from the Hovea-3 section, Western Australia

The temporal coincidence between the Late Permian mass extinction (LPME) and the emplacement of Siberian Trap basalts suggests a causal link between the two events. Here, we discuss stratigraphic changes of organic and inorganic (including isotopic) geochemical properties of marine sediments across the Permian–Triassic boundary (PTB) in the Hovea-3 core, Western Australia, a key PTB section in the southern Neo-Tethys ocean. These data are compared with published data from the Meishan section, southern China, and from the Opal Creek section in western Canada, providing a view of Tethys and Panthalassa changes at the PTB. Trace metal and N-isotopic data, together with organic matter properties suggest that anoxic conditions were established prior to the LPME, intensified close to the LPME, and continued with photic-zone euxinia into the Early Triassic. For the Hovea-3 section, Re-Os ages confirm Changhsingian (253.5 ± 1.4 Ma) deposition of the dated interval sampled immediately below the stratigraphic level characterized by major lithological and isotopic changes. Evaluation of Re-Os, N, and Hg elemental and isotopic data for Hovea-3 suggests that anoxic conditions in the latest Permian were generally unrelated to direct magmatic contributions. A major increase in the initial Os isotopic ratio of Lower Triassic shales suggest an ~8× increase in the Early Triassic continental runoff, based on moderately conservative assumptions for end-members contributing Os to the Permian–Triassic ocean. Comparison to other PTB sections confirms a global signal of increasing Re/Os ratios in the Late Permian, and major and long-lived changes in the isotopic composition of the post-extinction ocean. A distinct peak in Hg concentrations carrying a volcanic isotopic signature, also identified in other PTB sections, likely represents a major pulse of Siberian Trap volcanism. This Hg peak in the Hovea-3 section, however, is detected above the stratigraphic level containing multiple other widely recognized and more permanent geochemical changes. Therefore, direct volcanic inputs to the Permian–Triassic Ocean likely post-date the LPME in this Western Australian section

Extreme Ultra-Violet Spectroscopy of the Lower Solar Atmosphere During Solar Flares

The extreme ultraviolet portion of the solar spectrum contains a wealth of diagnostic tools for probing the lower solar atmosphere in response to an injection of energy, particularly during the impulsive phase of solar flares. These include temperature and density sensitive line ratios, Doppler shifted emission lines and nonthermal broadening, abundance measurements, differential emission measure profiles, and continuum temperatures and energetics, among others. In this paper I shall review some of the advances made in recent years using these techniques, focusing primarily on studies that have utilized data from Hinode/EIS and SDO/EVE, while also providing some historical background and a summary of future spectroscopic instrumentation.Comment: 34 pages, 8 figures. Submitted to Solar Physics as part of the Topical Issue on Solar and Stellar Flare

A comparison between the structures of reconstituted salivary pellicles and oral mucin (MUC5B) films

Hypothesis: Salivary pellicles i.e., thin films formed upon selective adsorption of saliva, protect oral surfaces against chemical and mechanical insults. Pellicles are also excellent aqueous lubricants. It is generally accepted that reconstituted pellicles have a two-layer structure, where the outer layer is mainly composed of MUC5B mucins. We hypothesized that by comparing the effect of ionic strength on reconstituted pellicles and MUC5B films we could gain further insight into the pellicle structure. Experiments: Salivary pellicles and MUC5B films reconstituted on solid surfaces were investigated at different ionic strengths by Force Spectroscopy, Quartz Crystal Microbalance with Dissipation, Null Ellipsometry and Neutron Reflectometry. Findings: Our results support the two-layer structure for reconstituted salivary pellicles. The outer layer swelled when ionic strength decreased, indicating a weak polyelectrolyte behavior. While initially the MUC5B films exhibited a similar tendency, this was followed by a drastic collapse indicating an interaction between exposed hydrophobic domains. This suggests that mucins in the pellicle outer layer form complexes with other salivary components that prevent this interaction. Lowering ionic strength below physiological values also led to a partial removal of the pellicle inner layer. Overall, our results highlight the importance that the interactions of mucins with other pellicle components play on their structure

Executive Summary:International Clinical Practice Guidelines for Pediatric Ventilator Liberation, A Pediatric Acute Lung Injury and Sepsis Investigators (PALISI) Network Document

Rationale: Pediatric-specific ventilator liberation guidelines are lacking despite the many studies exploring elements of extubation readiness testing. The lack of clinical practice guidelines has led to significant and unnecessary variation in methods used to assess pediatric patients’ readiness for extubation. Methods: Twenty-six international experts comprised a multiprofessional panel to establish pediatrics-specific ventilator liberation clinical practice guidelines, focusing on acutely hospitalized children receiving invasive mechanical ventilation for more than 24 hours. Eleven key questions were identified and first prioritized using the Modified Convergence of Opinion on Recommendations and Evidence. A systematic review was conducted for questions that did not meet an a priori threshold of &gt;80% agreement, with Grading of Recommendations, Assessment, Development, and Evaluation methodologies applied to develop the guidelines. The panel evaluated the evidence and drafted and voted on the recommendations. Measurements and Main Results: Three questions related to systematic screening using an extubation readiness testing bundle and a spontaneous breathing trial as part of the bundle met Modified Convergence of Opinion on Recommendations criteria of &gt;80% agreement. For the remaining eight questions, five systematic reviews yielded 12 recommendations related to the methods and duration of spontaneous breathing trials, measures of respiratory muscle strength, assessment of risk of postextubation upper airway obstruction and its prevention, use of postextubation noninvasive respiratory support, and sedation. Most recommendations were conditional and based on low to very low certainty of evidence. Conclusions: This clinical practice guideline provides a conceptual framework with evidence-based recommendations for best practices related to pediatric ventilator liberation.</p

Multi-isotopic study of the earliest medieval inhabitants of Santiago de Compostela (Galicia, Spain)

YesSantiago de Compostela is, together with Rome and Jerusalem, one of the three main pilgrimage and religious centres for Catholicism. The belief that the remains of St James the Great, one of the twelve apostles of Jesus Christ, is buried there has stimulated, since their reported discovery in the 9th century AD, a significant flow of people from across the European continent and beyond. Little is known about the practical experiences of people living within the city during its rise to prominence, however. Here, for the first time, we combine multi-isotope analysis (δ13C, δ15N, δ18Oap, δ13Cap, and 87Sr/86Sr) and radiocarbon dating (14C) of human remains discovered at the crypt of the Cathedral of Santiago to directly study changes in diet and mobility during the first three centuries of Santiago’s emergence as an urban centre (9th-12th centuries AD). Together with assessment of the existing archaeological data, our radiocarbon chronology broadly confirms historical tradition regarding the first occupation of the site. Isotopic analyses reveal that the foundation of the religious site attracted migrants from the wider region of the northwest corner of the Iberian Peninsula, and possibly from further afield. Stable isotope analysis of collagen, together with information on tomb typology and location, indicates that the inhabitants of the city experienced increasing socioeconomic diversity as it became wealthier as the hub of a wide network of pilgrimage. Our research represents the potential of multidisciplinary analyses to reveal insights into the origins and impacts of the emergence of early pilgrimage centres on the diets and status of communities within Christian medieval Europe and beyond.This project has been supported by a grant from the ‘la Caixa’ Banking Foundation (ID 100010434; Code: LCF/BQ/ES16/11570006). Patxi Pérez-Ramallo and Patrick Roberts would also like to thank the Max Planck Society for funding for this project. Patxi Pérez-Ramallo, Hannah Koon and Julia Beaumont would like to thank the University of Bradford for funding a support the first osteological and stable isotope analysis conducted in 2015. Two of the isotopic analyses and 14C dates have been carried out with funding from the Xunta de Galicia to the CulXeo Group (ED431B 2018/47) and to the research network ‘Cultural Heritage, archaeological and technical services’ (R2016/023). Open Access funding enabled and organized by Projekt DEAL

Health providers as bricoleurs:an examination of the adaption of health ecosystems to superdiversity in Europe

This article examines the ways in which healthcare providers from a mixed economy of welfare operating in superdiverse neighbourhoods connect and innovate across the healthcare ecosystem to meet diverse and complex needs. Moving beyond a health systems approach which siloes different types of provision, we use the concept of bricolage to make visible the work undertaken by providers across the ecosystem. While we show that public, private and civil society provision all adapt to meet complex and diverse needs to some degree, we highlight the importance of inter-connectedness between providers and note the role of civil society in addressing gaps and cracks in provision. The importance of adopting a whole ecosystem approach and focusing on the actions and interactions which enable the ecosystem to function in complex demographic environments is highlighted before we stress the dangers of over-reliance on civil society.info:eu-repo/semantics/acceptedVersio

Germline MBD4-deficiency causes a multi-tumor predisposition syndrome

We report an autosomal recessive, multi-organ tumor predisposition syndrome, caused by bi-allelic loss-of-function germline variants in the base excision repair (BER) gene MBD4. We identified five individuals with bi-allelic MBD4 variants within four families and these individuals had a personal and/or family history of adenomatous colorectal polyposis, acute myeloid leukemia, and uveal melanoma. MBD4 encodes a glycosylase involved in repair of G:T mismatches resulting from deamination of 5′-methylcytosine. The colorectal adenomas from MBD4-deficient individuals showed a mutator phenotype attributable to mutational signature SBS1, consistent with the function of MBD4. MBD4-deficient polyps harbored somatic mutations in similar driver genes to sporadic colorectal tumors, although AMER1 mutations were more common and KRAS mutations less frequent. Our findings expand the role of BER deficiencies in tumor predisposition. Inclusion of MBD4 in genetic testing for polyposis and multi-tumor phenotypes is warranted to improve disease management