Employment Characteristics And Mental Health: A Quantitative Study

Previous literature suggests that certain job characteristics may influence a person\u27s mental health in a negative way. Work is an important part of every American\u27s life. It is how they make money and can be how they identify themselves. This thesis examines that relationship further with new data and variables and also looks at the effect that the 2007 to 2009 U.S. recession had on increasing poor mental health among individuals. Americans\u27 jobs were influenced by this national recession in multiple ways. This thesis explores the relationship between the national economy and how job characteristics impact American\u27s poor mental health. Data from the 2006 (N=4510) and 2012 (N=1974) years of the General Social Survey were utilized, and OLS regression was used to test the hypotheses. The results indicate that the unemployed report more days of poor mental health, as well as a higher number of reported days of poor mental health after the recession. It was found that race, income, sex, education, age, marital status, and work stress also had a significant relationship with mental health. The implications of the results of this thesis suggest the need for more programs helping with the mental health of the unemployed. It also shows that the recession impacts a person\u27s work life, which influences his or her mental health, several years after the recession is ove

RHAPSODY-G simulations II - Baryonic growth and metal enrichment in massive galaxy clusters

We study the evolution of the stellar component and the metallicity of both the intracluster medium and of stars in massive ($M_{\rm vir}\approx 6\times 10^{14}$ M$_{\odot}/h$) simulated galaxy clusters from the Rhapsody-G suite in detail and compare them to observational results. The simulations were performed with the AMR code RAMSES and include the effect of AGN feedback at the sub-grid level. AGN feedback is required to produce realistic galaxy and cluster properties and plays a role in mixing material in the central regions and regulating star formation in the central galaxy. In both our low and high resolution runs with fiducial stellar yields, we find that stellar and ICM metallicities are a factor of two lower than in observations. We find that cool core clusters exhibit steeper metallicity gradients than non-cool core clusters, in qualitative agreement with observations. We verify that the ICM metallicities measured in the simulation can be explained by a simple "regulator" model in which the metallicity is set by a balance of stellar yield and gas accretion. It is plausible that a combination of higher resolution and higher metal yield in AMR simulation would allow the metallicity of simulated clusters to match observed values; however this hypothesis needs to be tested with future simulations. Comparison to recent literature highlights that results concerning the metallicity of clusters and cluster galaxies might depend sensitively on the scheme chosen to solve the hydrodynamics.Comment: 22 pages, 11 figures, 2 tables. Accepted for publication on MNRA

Rhapsody-G simulations: galaxy clusters as baryonic closed boxes and the covariance between hot gas and galaxies

Within a sufficiently large cosmic volume, conservation of baryons implies a simple `closed box' view in which the sum of the baryonic components must equal a constant fraction of the total enclosed mass. We present evidence from Rhapsody-G hydrodynamic simulations of massive galaxy clusters that the closed-box expectation may hold to a surprising degree within the interior, non-linear regions of haloes. At a fixed halo mass, we find a significant anti-correlation between hot gas mass fraction and galaxy mass fraction (cold gas + stars), with a rank correlation coefficient of -0.69 within $R_{500c}$. Because of this anti-correlation, the total baryon mass serves as a low-scatter proxy for total cluster mass. The fractional scatter of total baryon fraction scales approximately as $0.02 (\Delta_c/100)^{0.6}$, while the scatter of either gas mass or stellar mass is larger in magnitude and declines more slowly with increasing radius. We discuss potential observational tests using cluster samples selected by optical and hot gas properties; the simulations suggest that joint selection on stellar and hot gas has potential to achieve 5% scatter in total halo mass.Comment: 10 pages, 6 figures, 3 tables; replaced to match published versio

Rhapsody-G simulations: galaxy clusters as baryonic closed boxes and the covariance between hot gas and galaxies

Within a sufficiently large cosmic volume, conservation of baryons implies a simple ‘closed box' view in which the sum of the baryonic components must equal a constant fraction of the total enclosed mass. We present evidence from Rhapsody-G hydrodynamic simulations of massive galaxy clusters that the closed-box expectation may hold to a surprising degree within the interior, non-linear regions of haloes. At a fixed halo mass, we find a significant anti-correlation between hot gas mass fraction and galaxy mass fraction (cold gas+stars), with a rank correlation coefficient of −0.69 within R500c. Because of this anti-correlation, the total baryon mass serves as a low-scatter proxy for total cluster mass. The fractional scatter of total baryon fraction scales approximately as 0.02(Δc/100)0.6, while the scatter of either gas mass or stellar mass is larger in magnitude and declines more slowly with increasing radius. We discuss potential observational tests using cluster samples selected by optical and hot gas properties; the simulations suggest that joint selection on stellar and hot gas has potential to achieve 5 per cent scatter in total halo mas

Lowering of tumor interstitial fluid pressure reduces tumor cell proliferation in a xenograft tumor model

High tumor interstitial fluid pressure (TIFP) is a characteristic of most solid tumors. TIFP may hamper adequate uptake of macromolecular therapeutics in tumor tissue. In addition, TIFP generates mechanical forces affecting the tumor cortex, which might influence the growth parameters of tumor cells. This seems likely as, in other tissues (namely, blood vessels or the skin), mechanical stretch is known to trigger proliferation. Therefore, we hypothesize that TIFP-induced stretch modulates proliferation-associated parameters. Solid epithelial tumors (A431 and A549) were grown in Naval Medical Research Institute nude mice, generating a TIFP of about 10 mm Hg (A431) or 5 mm Hg (A549). Tumor drainage of the central cystic area led to a rapid decline of TIFP, together with visible relaxation of the tumor cortex. It was found by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot analysis that TIFP lowering yields a decreased phosphorylation of proliferation-associated p44/42 mitogen-activated protein kinase and tumor relaxation. In confirmation, immunohistochemical staining showed a decrease of tumor-associated proliferation marker Ki-67 after TIFP lowering. These data suggest that the mechanical stretch induced by TIFP is a positive modulator of tumor proliferation.publishedVersio

Increased plasma colloid osmotic pressure facilitates the uptake of therapeutic macromolecules in a xenograft tumour model

Elevated tumor interstitial fluid pressure (TIFP) is a characteristic of most solid tumors. Clinically, TIFP may hamper the uptake of chemotherapeutic drugs into the tumor tissue reducing their therapeutic efficacy. In this study, a means of modulating TIFP to increase the flux of macromolecules into tumor tissue is presented, which is based on the rationale that elevated plasma colloid osmotic pressure (COP) pulls water from tumor interstitium lowering the TIFP. Concentrated human serum albumin: (20% HSA), used as an agent to enhance COP, reduced the TIFP time-dependently from 8 to 2 mm Hg in human tumor xenograft models bearing A431 epidermoid vulva carcinomas. To evaluate whether this reduction facilitates the uptake of macromolecules, the intratumoral distribution of fluorescently conjugated dextrans (2.5 mg/ml) and cetuximab (2.0 mg/ml) was probed using novel time domain nearinfrared fluorescence imaging. This method permitted discrimination and semiquantification of tumor-accumulated conjugate from background and unspecific probe fluorescence. The coadministration of 20% HSA together with either dextrans or cetuximab was found to lower the TIFP significantly and increase the concentration of the substances within the tumor tissue in comparison to control tumors. Furthermore, combined administration of 20%HSA plus cetuximab reduced the tumor growth significantly in comparison to standard cetuximab treatment. These data demonstrate that increased COP lowers the TIFP within hours and increases the uptake of therapeutic macromolecules into the tumor interstitium leading to reduced tumor growth. This model represents a novel approach to facilitate the delivery of therapeutics into tumor tissue, particularly monoclonal antibodies

Increased plasma colloid osmotic pressure facilitates the uptake of therapeutic macromolecules in a xenograft tumour model

Elevated tumor interstitial fluid pressure (TIFP) is a characteristic of most solid tumors. Clinically, TIFP may hamper the uptake of chemotherapeutic drugs into the tumor tissue reducing their therapeutic efficacy. In this study, a means of modulating TIFP to increase the flux of macromolecules into tumor tissue is presented, which is based on the rationale that elevated plasma colloid osmotic pressure (COP) pulls water from tumor interstitium lowering the TIFP. Concentrated human serum albumin: (20% HSA), used as an agent to enhance COP, reduced the TIFP time-dependently from 8 to 2 mm Hg in human tumor xenograft models bearing A431 epidermoid vulva carcinomas. To evaluate whether this reduction facilitates the uptake of macromolecules, the intratumoral distribution of fluorescently conjugated dextrans (2.5 mg/ml) and cetuximab (2.0 mg/ml) was probed using novel time domain nearinfrared fluorescence imaging. This method permitted discrimination and semiquantification of tumor-accumulated conjugate from background and unspecific probe fluorescence. The coadministration of 20% HSA together with either dextrans or cetuximab was found to lower the TIFP significantly and increase the concentration of the substances within the tumor tissue in comparison to control tumors. Furthermore, combined administration of 20%HSA plus cetuximab reduced the tumor growth significantly in comparison to standard cetuximab treatment. These data demonstrate that increased COP lowers the TIFP within hours and increases the uptake of therapeutic macromolecules into the tumor interstitium leading to reduced tumor growth. This model represents a novel approach to facilitate the delivery of therapeutics into tumor tissue, particularly monoclonal antibodies.publishedVersio

Entwicklung eines transplantierbaren Hautäquivalentes auf Basis von Matriderm mit menschlichen Keratinozyten und Fibroblasten

Meeting Abstract Es wurde eine zellbasierte Wundauflage mit Keratinozyten und Fibroblasten auf Basis einer kommerziellen Wundauflage (Matriderm, Collagen/Elastin-Matrix) generiert, um damit großflächige Verbrennungswunden behandeln zu können. Zunächst wurde die Expansion der Keratinozyten optimiert und die Zeit für die Anzüchtung minimiert. Ausgangsmaterial waren 1–2 cm2 Spalthaut vom Patienten. Epidermis und Dermis wurden nach einer enzymatischen Behandlung mit Thermolysin voneinander getrennt. Aus den beiden Hautkompartimenten wurden durch Trypsin- und Kollagenase I-Behandlung Keratinozyten und Fibroblasten isoliert, welche in Kollagen I-beschichteten Zellkulturflaschen expandiert wurden. Nach 10 Tagen wurden die Fibroblasten auf 100 cm2 Matriderm aufgebracht. Nach einwöchiger submerser Kultivierung wurden die Keratinozyten ausgesät. Eine Woche später wurde die Matrix an die Luft-Flüssigkeitsgrenze angehoben, um die epidermale Differenzierung einzuleiten. Nach 16 Tagen wurde das Hautäquivalent fixiert und immunhistologisch sowie elektronen-mikroskopisch begutachtet. Die Histologie zeigte eine regelgerechte Stratifizierung des epidermalen Anteils. Immunhistologisch ließ sich eine Basalmembran mit Collagen IV und Laminin 5 nachweisen. Proliferative Zellen, nachgewiesen mit KI-67 befanden sich lediglich in der basalen Region der Epidermis. Desmoglein, sowie die Differenzierungsmarker Involucrin und CK 10 wurden suprabasal nachgewiesen. Elektronenmikroskopisch waren die Basalmembran sowie die Zell-Zell-Verbindungen in Form von Desmosmen zu erkennen. Späte Differenzierungsmerkmale, wie granuläre Strukturen und verdickte Zellmembranen, fanden sich im Str. granulosum und Str. corneum. Die Studie zeigt, dass man aus Matriderm eine zellbasierte Wundauflage herstellen kann, die verglichen mit dem Ausgangsmaterial um den Faktor 50–100 vergrößert ist und deren Aufbau normaler Haut weitgehend entspricht

Assessing and augmenting SCADA cyber security: a survey of techniques

SCADA systems monitor and control critical infrastructures of national importance such as power generation and distribution, water supply, transportation networks, and manufacturing facilities. The pervasiveness, miniaturisations and declining costs of internet connectivity have transformed these systems from strictly isolated to highly interconnected networks. The connectivity provides immense benefits such as reliability, scalability and remote connectivity, but at the same time exposes an otherwise isolated and secure system, to global cyber security threats. This inevitable transformation to highly connected systems thus necessitates effective security safeguards to be in place as any compromise or downtime of SCADA systems can have severe economic, safety and security ramifications. One way to ensure vital asset protection is to adopt a viewpoint similar to an attacker to determine weaknesses and loopholes in defences. Such mind sets help to identify and fix potential breaches before their exploitation. This paper surveys tools and techniques to uncover SCADA system vulnerabilities. A comprehensive review of the selected approaches is provided along with their applicability

Advances in predicting acute GVHD

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/96282/1/bjh12142.pd