Untersuchungen zur Vererbung von Resistenzeigenschaften bei Reben gegen Oidium tuckeri, Plasmopara viticola und Botrytis cinerea

Investigations on the heritability of resistance to Oidium tuclceri, Plasmopara viticolaand Botrytis cinerea in grapesIn the years 1985-1987 investigations on progenies of interspecific crossings were carried out in order to ascertain the heritability of resistance to Oidium tuckeri, Plasmopara viticola and Botrytis cinerea (formation of stilbene). Results from testing the resistance in the field and in vitro were closeley correlated and allowed therefore to check the degree of resistance in vitro under standardized conditions. The coefficients of heritability in the narrow sense calculated for 0. tuckeri range from 0.31 to 0.51, for P. viticola from 0.26 to 0.39 and for the formation of stilbene from 0.23 to 0.26. According to these results, the resistance to 0. tuckeri is most strongly influenced by additive gene effects. The high coefficients of heritability in the broad sense lying between 0.82 and 0.95 for all the investigated characters indicate little environmental dependence.Investigations on a seed!ing population of the Asian wild species Vitis yeshanensis point to a monogenic respectively oligogenic mechanism of resistance. The usage of this resistance for breeding purposes as a complement to the establishment of a stable horizontal resistance is discussed

Does the future look bright? Processing style determines the impact of valence weighting biases and self-beliefs on expectations

People regularly form expectations about their future, and whether those expectations are positive or negative can have important consequences. So, what determines the valence of people’s expectations? Research seeking to answer this question by using an individual-differences approach has established that trait biases in optimistic/pessimistic self-beliefs and, more recently, trait biases in behavioral tendencies to weight one’s past positive versus negative experiences more heavily each predict the valence of people’s typical expectations. However, these two biases do not correlate, suggesting limits on a purely individual-differences approach to predicting people’s expectations. We hypothesize that, because these two biases appear to operate via distinct processes (with self-beliefs operating top-down and valence weighting bias operating bottom-up), to predict a person’s expectations on a given occasion, it is also critical to consider situational factors influencing processing style. To test this hypothesis, we investigated how an integral part of future thinking that influences processing style—mental imagery—determines each bias’s influence. Two experiments measured valence weighting biases and optimistic/pessimistic self-beliefs, then manipulated whether participants formed expectations using their own first-person visual perspective (which facilitates bottom-up processes) or an external third-person visual perspective (which facilitates top-down processes). Expectations corresponded more with valence weighting biases from the first-person (vs. third-person) but more with self-beliefs from the third-person (vs. first-person). Two additional experiments manipulated valence weighting bias, demonstrating its causal role in shaping expectations (and behaviors) with first-person, but not third-person, imagery. These results suggest the two biases operate via distinct processes, holding implications for interventions to increase optimism

Chlamydiae in febrile children with respiratory tract symptoms and age-matched controls, Ghana.

Members of the javax.xml.bind.JAXBElement@d71ef36 order are obligate intracellular pathogens causing acute and chronic infectious diseases. javax.xml.bind.JAXBElement@1d3522b1 are established agents of community- and zoonotically acquired respiratory tract infections, and emerging pathogens among the javax.xml.bind.JAXBElement@378e5899 -related bacteria have been implicated in airway infections. The role of both in airway infections in Africa is underexplored. We performed a case -control study on the prevalence of javax.xml.bind.JAXBElement@12f1b6f0 and javax.xml.bind.JAXBElement@10fbf3c7 -related emerging pathogens in children with febrile respiratory tract infections in West Africa, Ghana. Using a pan- javax.xml.bind.JAXBElement@246f6bc3 broad-range real-time PCR, we detected chlamydial DNA in 11 (1.9%) of 572 hospitalized febrile children with respiratory tract symptoms and in 24 (4.3%) of 560 asymptomatic age-matched controls (p 0.03). javax.xml.bind.JAXBElement@6160b547 were found to be common among both symptomatic and healthy Ghanaian children, with javax.xml.bind.JAXBElement@7e39c9ad being the most prevalent species. javax.xml.bind.JAXBElement@31136866 were detected in two children without symptoms but not in the symptomatic group. We identified neither javax.xml.bind.JAXBElement@6e7d2cf4 nor javax.xml.bind.JAXBElement@ad366f1 but a member of a new chlamydial family that shared 90.2% sequence identity with the 16S rRNA gene of the zoonotic pathogen javax.xml.bind.JAXBElement@3a17b2a7 . In addition, we found a new javax.xml.bind.JAXBElement@10edaddb -related species that belonged to a novel family sharing 91.3% 16S rRNA sequence identity with javax.xml.bind.JAXBElement@177039be Syngnamydia venezia. The prevalence and spectrum of chlamydial species differed from previous results obtained from children of other geographic regions and our study indicates that both, javax.xml.bind.JAXBElement@605214f and javax.xml.bind.JAXBElement@18029a2 -related bacteria, are not clearly linked to clinical symptoms in Ghanaian children

Anthroponotic transmission of Cryptosporidium parvum predominates in countries with poorer sanitation - a systematic review and meta-analysis

Background: Globally cryptosporidiosis is one of the commonest causes of mortality in children under 24 months old and may be associated with important longterm health effects. Whilst most strains of Cryptosporidium parvum are zoonotic, C. parvum IIc is almost certainly anthroponotic. The global distribution of this potentially important emerging infection is not clear. Methods: We conducted a systematic review of papers identifying the subtype distribution of C. parvum infections globally. We searched PubMed and Scopus using the following key terms Cryptospor* AND parvum AND (genotyp* OR subtyp* OR gp60). Studies were eligible for inclusion if they had found C. parvum within their human study population and had subtyped some or all of these samples using standard gp60 subtyping. Pooled analyses of the proportion of strains being of the IIc subtype were determined using StatsDirect. Meta-regression analyses were run to determine any association between the relative prevalence of IIc and Gross Domestic Product, proportion of the population with access to improved drinking water and improved sanitation. Results: From an initial 843 studies, 85 were included in further analysis. Cryptosporidium parvum IIc was found in 43 of these 85 studies. Across all studies the pooled estimate of relative prevalence of IIc was 19.0% (95% CI: 12.9–25.9%), but there was substantial heterogeneity. In a meta-regression analysis, the relative proportion of all C. parvum infections being IIc decreased as the percentage of the population with access to improved sanitation increased and was some 3.4 times higher in those studies focussing on HIV-positive indivduals. Conclusions: The anthroponotic C. parvum IIc predominates primarily in lower-income countries with poor sanitation and in HIV-positive individuals. Given the apparent enhanced post-infectious virulence of the other main anthroponotic species of Cryptosporidium (C. hominis), it is important to learn about the impact of this subtype on human health

Anthroponotic transmission of Cryptosporidium parvum predominates in countries with poorer sanitation - a systematic review and meta-analysis

Background: Globally cryptosporidiosis is one of the commonest causes of mortality in children under 24 months old and may be associated with important longterm health effects. Whilst most strains of Cryptosporidium parvum are zoonotic, C. parvum IIc is almost certainly anthroponotic. The global distribution of this potentially important emerging infection is not clear. Methods: We conducted a systematic review of papers identifying the subtype distribution of C. parvum infections globally. We searched PubMed and Scopus using the following key terms Cryptospor* AND parvum AND (genotyp* OR subtyp* OR gp60). Studies were eligible for inclusion if they had found C. parvum within their human study population and had subtyped some or all of these samples using standard gp60 subtyping. Pooled analyses of the proportion of strains being of the IIc subtype were determined using StatsDirect. Meta-regression analyses were run to determine any association between the relative prevalence of IIc and Gross Domestic Product, proportion of the population with access to improved drinking water and improved sanitation. Results: From an initial 843 studies, 85 were included in further analysis. Cryptosporidium parvum IIc was found in 43 of these 85 studies. Across all studies the pooled estimate of relative prevalence of IIc was 19.0% (95% CI: 12.9–25.9%), but there was substantial heterogeneity. In a meta-regression analysis, the relative proportion of all C. parvum infections being IIc decreased as the percentage of the population with access to improved sanitation increased and was some 3.4 times higher in those studies focussing on HIV-positive indivduals. Conclusions: The anthroponotic C. parvum IIc predominates primarily in lower-income countries with poor sanitation and in HIV-positive individuals. Given the apparent enhanced post-infectious virulence of the other main anthroponotic species of Cryptosporidium (C. hominis), it is important to learn about the impact of this subtype on human health

Intestinal Microbiota Composition of Interleukin-10 Deficient C57BL/6J Mice and Susceptibility to Helicobacter hepaticus-Induced Colitis

The mouse pathobiont Helicobacter hepaticus can induce typhlocolitis in interleukin-10-deficient mice, and H. hepaticus infection of immunodeficient mice is widely used as a model to study the role of pathogens and commensal bacteria in the pathogenesis of inflammatory bowel disease. C57BL/6J Il10[superscript −/−] mice kept under specific pathogen-free conditions in two different facilities (MHH and MIT), displayed strong differences with respect to their susceptibilities to H. hepaticus-induced intestinal pathology. Mice at MIT developed robust typhlocolitis after infection with H. hepaticus, while mice at MHH developed no significant pathology after infection with the same H. hepaticus strain. We hypothesized that the intestinal microbiota might be responsible for these differences and therefore performed high resolution analysis of the intestinal microbiota composition in uninfected mice from the two facilities by deep sequencing of partial 16S rRNA amplicons. The microbiota composition differed markedly between mice from both facilities. Significant differences were also detected between two groups of MHH mice born in different years. Of the 119 operational taxonomic units (OTUs) that occurred in at least half the cecum or colon samples of at least one mouse group, 24 were only found in MIT mice, and another 13 OTUs could only be found in MHH samples. While most of the MHH-specific OTUs could only be identified to class or family level, the MIT-specific set contained OTUs identified to genus or species level, including the opportunistic pathogen, Bilophila wadsworthia. The susceptibility to H. hepaticus-induced colitis differed considerably between Il10[superscript −/−] mice originating from the two institutions. This was associated with significant differences in microbiota composition, highlighting the importance of characterizing the intestinal microbiome when studying murine models of IBD.National Institutes of Health (U.S.) (Grant NIH P01-CA26731)National Institutes of Health (U.S.) (Grant NIH P30ES0026731)National Institutes of Health (U.S.) (Grant NIH R01-OD011141

Genomic ancestry estimation quantifies use of wild species in grape breeding

BACKGROUND: Grapes are one of the world’s most valuable crops and most are made into wine. Grapes belong to the genus Vitis, which includes over 60 inter-fertile species. The most common grape cultivars derive their entire ancestry from the species Vitis vinifera, but wild relatives have also been exploited to create hybrid cultivars, often with increased disease resistance. RESULTS: We evaluate the genetic ancestry of some of the most widely grown commercial hybrids from North America and Europe. Using genotyping-by-sequencing (GBS), we generated 2482 SNPs and 56 indels from 7 wild Vitis, 7 V. vinifera, and 64 hybrid cultivars. We used a principal component analysis (PCA) based ancestry estimation procedure and verified its accuracy with both empirical and simulated data. V. vinifera ancestry ranged from 11 % to 76 % across hybrids studied. Approximately one third (22/64) of the hybrids have ancestry estimates consistent with F1 hybridization: they derive half of their ancestry from wild Vitis and half from V. vinifera. CONCLUSIONS: Our results suggest that hybrid grape breeding is in its infancy. The distribution of V. vinifera ancestry across hybrids also suggests that backcrosses to wild Vitis species have been more frequent than backcrosses to V. vinifera during hybrid grape breeding. This pattern is unusual in crop breeding, as it is most common to repeatedly backcross to elite, or domesticated, germplasm. We anticipate our method can be extended to facilitate marker-assisted selection in order to introgress beneficial wild Vitis traits, while allowing for offspring with the highest V. vinifera content to be selected at the seedling stage. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2834-8) contains supplementary material, which is available to authorized users

High Prevalence of Mycoplasma pneumoniae and Chlamydia pneumoniae in Children with Acute Respiratory Infections from Lima, Peru

Background Mycoplasma pneumoniae and Chlamydia pneumoniae are atypical pathogens responsible for pneumonia and a leading cause of morbidity and mortality in low income countries. The study objective is to determine the prevalence of this pathogens in Peruvian children with acute respiratory infections. Methods A consecutive cross-sectional study was conducted in Lima, Peru from May 2009 to September 2010. A total of 675 children admitted with clinical diagnoses of acute respiratory infections were tested for Mycoplasma pneumoniae and Chlamydia pneumoniae detection by polymerase chain reaction (PCR), and clinical symptoms were registered by the attending physician. Results Mycoplasma pneumonia was detected in 25.19% (170/675) of nasopharyngeal samples and Chlamydia pneumonia in 10.52% (71/675). The most common symptoms in patients with these atypical pathogens were rhinorrhea, cough and fever. A higher prevalence of Mycoplasma pneumoniae cases were registered in summer, between December 2009 and March 2010. Conclusions Mycoplasma pneumoniae and Chlamydia pneumonia are a significant cause of morbidity in Peruvian children with acute respiratory infections (ARI). Further studies should evaluate the use of reliable techniques such as PCR in Peru in order to avoid underdiagnoses of these atypical pathogens