4,961 research outputs found
Bulk de novo mitogenome assembly from pooled total DNA elucidates the phylogeny of weevils (Coleoptera: Curculionoidea)
Complete mitochondrial genomes have been shown to be reliable markers for phylogeny reconstruction among diverse animal groups. However, the relative difficulty and high cost associated with obtaining de novo full mitogenomes have frequently led to conspicuously low taxon sampling in ensuing studies. Here, we report the successful use of an economical and accessible method for assembling complete or near-complete mitogenomes through shot-gun next-generation sequencing of a single library made from pooled total DNA extracts of numerous target species. To avoid the use of separate indexed libraries for each specimen, and an associated increase in cost, we incorporate standard polymerase chain reaction-based “bait” sequences to identify the assembled mitogenomes. The method was applied to study the higher level phylogenetic relationships in the weevils (Coleoptera: Curculionoidea), producing 92 newly assembled mitogenomes obtained in a single Illumina MiSeq run. The analysis supported a separate origin of wood-boring behavior by the subfamilies Scolytinae, Platypodinae, and Cossoninae. This finding contradicts morphological hypotheses proposing a close relationship between the first two of these but is congruent with previous molecular studies, reinforcing the utility of mitogenomes in phylogeny reconstruction. Our methodology provides a technically simple procedure for generating densely sampled trees from whole mitogenomes and is widely applicable to groups of animals for which bait sequences are the only required prior genome knowledge
Pleiotropy of FRIGIDA enhances the potential for multivariate adaptation.
An evolutionary response to selection requires genetic variation; however, even if it exists, then the genetic details of the variation can constrain adaptation. In the simplest case, unlinked loci and uncorrelated phenotypes respond directly to multivariate selection and permit unrestricted paths to adaptive peaks. By contrast, 'antagonistic' pleiotropic loci may constrain adaptation by affecting variation of many traits and limiting the direction of trait correlations to vectors that are not favoured by selection. However, certain pleiotropic configurations may improve the conditions for adaptive evolution. Here, we present evidence that the Arabidopsis thaliana gene FRI (FRIGIDA) exhibits 'adaptive' pleiotropy, producing trait correlations along an axis that results in two adaptive strategies. Derived, low expression FRI alleles confer a 'drought escape' strategy owing to fast growth, low water use efficiency and early flowering. By contrast, a dehydration avoidance strategy is conferred by the ancestral phenotype of late flowering, slow growth and efficient water use during photosynthesis. The dehydration avoidant phenotype was recovered when genotypes with null FRI alleles were transformed with functional alleles. Our findings indicate that the well-documented effects of FRI on phenology result from differences in physiology, not only a simple developmental switch
‘Ethnic group’, the state and the politics of representation
The assertion, even if only by implication, that ‘ethnic group’ categories represent ‘real’ tangible entities, indeed identities, is commonplace not only in the realms of political and policy discourse but also amongst contemporary social scientists. This paper, following Brubaker (2002), questions this position in a number of key respects: of these three issues will dominate the discussion that follows.
First, there is an interrogation of the proposition that those to whom the categories/labels refer constitute sociologically meaningful ‘groups’ as distinct from (mere) human collectivities. Secondly, there is the question of how these categories emerge, i.e. exactly what series of events, negotiations and contestations lie behind their construction and social acceptance. Thirdly, and as a corollary to the latter point, we explore the process of reification that leads to these categories being seen to represent ‘real things in the world’ (ibid.)
Pairing in the iron arsenides: a functional RG treatment
We study the phase diagram of a microscopic model for the superconducting
iron arsenides by means of a functional renormalization group. Our treatment
establishes a connection between a strongly simplified two-patch model by
Chubukov et al. and a five-band- analysis by Wang et al.. For a wide parameter
range, the dominant pairing instability occurs in the extended s-wave channel.
The results clearly show the relevance of pair scattering between electron and
hole pockets. We also give arguments that the phase transition between the
antiferromagnetic phase for the undoped system and the superconducting phase
may be first order
Asymptotic power law of moments in a random multiplicative process with weak additive noise
It is well known that a random multiplicative process with weak additive
noise generates a power-law probability distribution. It has recently been
recognized that this process exhibits another type of power law: the moment of
the stochastic variable scales as a function of the additive noise strength. We
clarify the mechanism for this power-law behavior of moments by treating a
simple Langevin-type model both approximately and exactly, and argue this
mechanism is universal. We also discuss the relevance of our findings to noisy
on-off intermittency and to singular spatio-temporal chaos recently observed in
systems of non-locally coupled elements.Comment: 11 pages, 9 figures, submitted to Phys. Rev.
Satellite Radiation Products for Ocean Biology and Biogeochemistry: Needs, State-of-the-Art, Gaps, Development Priorities, and Opportunities
Knowing the spatial and temporal distribution of the underwater light field, i.e., the spectral and angular structure of the radiant intensity at any point in the water column, is essential to understanding the biogeochemical processes that control the composition and evolution of aquatic ecosystems and their impact on climate and reaction to climate change. At present, only a few properties are reliably retrieved from space, either directly or via water-leaving radiance. Existing satellite products are limited to planar photosynthetically available radiation (PAR) and ultraviolet (UV) irradiance above the surface and diffuse attenuation coefficient. Examples of operational products are provided, and their advantages and drawbacks are examined. The usefulness and convenience of these products notwithstanding, there is a need, as expressed by the user community, for other products, i.e., sub-surface planar and scalar fluxes, average cosine, spectral fluxes (UV to visible), diurnal fluxes, absorbed fraction of PAR by live algae (APAR), surface albedo, vertical attenuation, and heating rate, and for associating uncertainties to any product on a pixel-by-pixel basis. Methodologies to obtain the new products are qualitatively discussed in view of most recent scientific knowledge and current and future satellite missions, and specific algorithms are presented for some new products, namely sub-surface fluxes and average cosine. A strategy and roadmap (short, medium, and long term) for usage and development priorities is provided, taking into account needs and readiness level. Combining observations from satellites overpassing at different times and geostationary satellites should be pursued to improve the quality of daily-integrated radiation fields, and products should be generated without gaps to provide boundary conditions for general circulation and biogeochemical models. Examples of new products, i.e., daily scalar PAR below the surface, daily average cosine for PAR, and sub-surface spectral scalar fluxes are presented. A procedure to estimate algorithm uncertainties in the total uncertainty budget for above-surface daily PAR, based on radiative simulations for expected situations, is described. In the future, space-borne lidars with ocean profiling capability offer the best hope for improving our knowledge of sub-surface fields. To maximize temporal coverage, space agencies should consider placing ocean-color instruments in L1 orbit, where the sunlit part of the Earth can be frequently observed
Fundamental scaling laws of on-off intermittency in a stochastically driven dissipative pattern forming system
Noise driven electroconvection in sandwich cells of nematic liquid crystals
exhibits on-off intermittent behaviour at the onset of the instability. We
study laser scattering of convection rolls to characterize the wavelengths and
the trajectories of the stochastic amplitudes of the intermittent structures.
The pattern wavelengths and the statistics of these trajectories are in
quantitative agreement with simulations of the linearized electrohydrodynamic
equations. The fundamental distribution law for the durations
of laminar phases as well as the power law of the amplitude distribution
of intermittent bursts are confirmed in the experiments. Power spectral
densities of the experimental and numerically simulated trajectories are
discussed.Comment: 20 pages and 17 figure
Ethnic Minorities and their Health Needs: Crisis of Perception and Behaviours
There is considerable evidence to suggest that racial and ethnic disparities exist in the provision of emergency and wider healthcare. The importance of collecting patient ethnic data has received attention in literature across the world and eliminating ethnic and racial health equalities is one of the primary aims of healthcare providers internationally. The poor health status of certain racial and ethnic groups has been well documented. The improvement of racial and ethnic disparities in healthcare is at the forefront of many public health agendas. This article addresses important policy, practice, and cultural issues confronted by the pre-hospital emergency care setup. This aspect of care plays a unique role in the healthcare safety net in providing a service to a very diverse population, including members of ethnic and racial minorities. Competent decision making by the emergency care practitioners requires patient-specific information and the health provider's prior medical knowledge and clinical training. The article reviews the current ethnicity trends in the UK along with international evidence linking ethnicity and health inequalities. The study argues that serious difficulties will arise between the health provider and the patient if they come from different backgrounds and therefore experience difficulties in cross-cultural communication. This adversely impacts on the quality of diagnostic and clinical decision making for minority patients. The article offers few strategies to address health inequalities in emergency care and concludes by arguing that much more needs to be done to ensure that we are hearing the voices of more diverse groups, groups who are often excluded from engagement through barriers such as language or mobility difficulties
Sensitivity of the superconducting state and magnetic susceptibility to key aspects of electronic structure in ferropnictides
Experiments on the iron-pnictide superconductors appear to show some
materials where the ground state is fully gapped, and others where low-energy
excitations dominate, possibly indicative of gap nodes. Within the framework of
a 5-orbital spin fluctuation theory for these systems, we discuss how changes
in the doping, the electronic structure or interaction parameters can tune the
system from a fully gapped to nodal sign-changing gap with s-wave ()
symmetry (). In particular we focus on the role of the hole pocket at
the point of the unfolded Brillouin zone identified as crucial to
the pairing by Kuroki {\it et al.}, and show that its presence leads to
additional nesting of hole and electron pockets which stabilizes the isotropic
state. The pocket's contribution to the pairing can be tuned by doping,
surface effects, and by changes in interaction parameters, which we examine.
Analytic expressions for orbital pairing vertices calculated within the RPA
fluctuation exchange approximation allow us to draw connections between aspects
of electronic structure, interaction parameters, and the form of the
superconducting gap
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