Field Evaluation of a Rising Plate Meter to Estimate Herbage Mass in Austrian Pastures

Pasture management is an important topic for dairy farms with grazing systems. Herbage mass (HM) is a key measure, and estimations of HM content in pastures allow for informed decisions in pasture management. A common method of estimating the HM content in pastures requires manually collected grass samples, which are subjected to laboratory analysis to determine the dry matter (DM) content. However, in recent years, new methods have emerged that generate digital data and aim to expedite, facilitate and improve the measurement of HM. This study aimed to evaluate the accuracy of a rising plate meter (RPM) tool in a practical setting to estimate HM in Austrian pastures. With this study, we also attempted to answer whether the tool is ready for use by farmers with its default settings. This study was conducted on the teaching and research farm of the University of Veterinary Medicine in Vienna, Austria. Data were collected from May to October 2021 in five different pastures. To evaluate the accuracy of the RPM tool, grass samples were collected and dried in an oven to extract their DM and calculate the HM. The HM obtained from the grass samples was used as the gold standard for this study. In total, 3796 RPM measurements and 203 grass samples yielding 49 measurement points were used for the evaluation of the RPM tool. Despite the differences in pasture composition, the averaged HM from the RPM tool showed a strong correlation with the gold standard (R2 = 0.73, rp = 0.86, RMSE = 517.86, CV = 33.67%). However, the results may not be good enough to justify the use of the tool, because simulations in economic studies suggest that the error of prediction should be lower than 15%. Furthermore, in some pastures, the RPM obtained poor results, indicating an additional need for pasture-specific calibrations, which complicates the use of the RPM tool

Typechecking XQuery: A Prototype in ASF+SDF

Semistructured data (particularly XML) are the standard data representation for information exchange in the world-wide web. A number of query languages for XML has been proposed. Most of them follow the style of SQL. One of these languages is XQuery. In this work, we propose the construction of a prototype for the static type analysis of XQuery programs. The prototype implements XQuery operational semantics, in a way that is close to that proposed by theW3C. The implementation was built using the ASF+SDF meta-environment. The prototype described here is a first step in the construction of a practical XML query language laboratory, in which different semantics for commands may be tested

Austrian Raw-Milk Hard-Cheese Ripening Involves Successional Dynamics of Non-Inoculated Bacteria and Fungi

Cheese ripening involves successional changes of the rind microbial composition that harbors a key role on the quality and safety of the final products. In this study, we analyzed the evolution of the rind microbiota (bacteria and fungi) throughout the ripening of Austrian Vorarlberger Bergkäse (VB), an artisanal surface-ripened cheese, by using quantitative and qualitative approaches. The real-time quantitative PCR results revealed that bacteria were more abundant than fungi in VB rinds throughout ripening, although both kingdoms were abundant along the process. The qualitative investigation was performed by high-throughput gene-targeted (amplicon) sequencing. The results showed dynamic changes of the rind microbiota throughout ripening. In the fresh products, VB rinds were dominated by Staphylococcus equorum and Candida. At early ripening times (14–30 days) Psychrobacter and Debaryomyces flourished, although their high abundance was limited to these time points. At the latest ripening times (90–160 days), VB rinds were dominated by S. equorum, Brevibacterium, Corynebacterium, and Scopulariopsis. Strong correlations were shown for specific bacteria and fungi linked to specific ripening periods. This study deepens our understanding of VB ripening and highlights different bacteria and fungi associated to specific ripening periods which may influence the organoleptic properties of the final products

The two-pore channel TPCN2 mediates NAADP-dependent Ca2+-release from lysosomal stores

Second messenger-induced Ca2+-release from intracellular stores plays a key role in a multitude of physiological processes. In addition to 1,4,5-inositol trisphosphate (IP3), Ca2+, and cyclic ADP ribose (cADPR) that trigger Ca2+-release from the endoplasmatic reticulum (ER), nicotinic acid adenine dinucleotide phosphate (NAADP) has been identified as a cellular metabolite that mediates Ca2+-release from lysosomal stores. While NAADP-induced Ca2+-release has been found in many tissues and cell types, the molecular identity of the channel(s) conferring this release remained elusive so far. Here, we show that TPCN2, a novel member of the two-pore cation channel family, displays the basic properties of native NAADP-dependent Ca2+-release channels. TPCN2 transcripts are widely expressed in the body and encode a lysosomal protein forming homomers. TPCN2 mediates intracellular Ca2+-release after activation with low-nanomolar concentrations of NAADP while it is desensitized by micromolar concentrations of this second messenger and is insensitive to the NAADP analog nicotinamide adenine dinucleotide phosphate (NADP). Furthermore, TPCN2-mediated Ca2+-release is almost completely abolished when the capacity of lysosomes for storing Ca2+ is pharmacologically blocked. By contrast, TPCN2-specific Ca2+-release is unaffected by emptying ER-based Ca2+ stores. In conclusion, these findings indicate that TPCN2 is a major component of the long-sought lysosomal NAADP-dependent Ca2+-release channel

Molecular Characterization of a Novel Intracellular ADP-Ribosyl Cyclase

Background. ADP-ribosyl cyclases are remarkable enzymes capable of catalyzing multiple reactions including the synthesis of the novel and potent intracellular calcium mobilizing messengers, cyclic ADP-ribose and NAADP. Not all ADP-ribosyl cyclases however have been characterized at the molecular level. Moreover, those that have are located predominately at the outer cell surface and thus away from their cytosolic substrates. Methodology/Principal Findings. Here we report the molecular cloning of a novel expanded family of ADP-ribosyl cyclases from the sea urchin, an extensively used model organism for the study of inositol trisphosphate-independent calcium mobilization. We provide evidence that one of the isoforms (SpARC1) is a soluble protein that is targeted exclusively to the endoplasmic reticulum lumen when heterologously expressed. Catalytic activity of the recombinant protein was readily demonstrable in crude cell homogenates, even under conditions where luminal continuity was maintained. Conclusions/Significance. Our data reveal a new intracellular location for ADP-ribosyl cyclases and suggest that production of calcium mobilizing messengers may be compartmentalized

Benefits of combined quantitative and qualitative evaluation of learning experience in a gerodontology course for dental students.

OBJECTIVES The oral health status of long-term care (LTC) facility residents is often poor, and acceptance of dental services by the elderly is irregular and mostly problem-driven. The perceived knowledge gap due to insufficient under- or postgraduate education and training in gerodontology might present a barrier for dentists to provide domiciliary care. This study aimed to develop a high-quality student course in gerodontology. METHODS A total of 52 undergraduate dental students (age: 23.4 ± 2.1 yrs., 81% female) participated in a novel one-year gerodontology course and were included in this prospective study. The course was organized over two semesters, comprising two consecutive modules (one theoretical and one practical). The evaluation after the first semester applied a 16-item questionnaire with an ordinal 6-point response scale ranging from "not satisfied at all" (0) to "very satisfied" (5) for quantitative evaluation, and free-text comments as the qualitative part. These qualitative findings were used for validating the satisfaction questionnaire by triangulation, and to identify potential issues for improving the course. Satisfaction scores of the second evaluation after 1 year were used to assess potential effects of course modifications by comparing the participant satisfaction scores between both evaluations. RESULTS Satisfaction scores of 3.6 ± 0.7 after the first semester indicated students' initial satisfaction. The lowest satisfaction was observed for timeframe (2.6 ± 1.3) and interdisciplinary education (3.0 ± 1.4). The qualitative evaluation confirmed not only the ratings but also provided potential explanations, which were addressed by modifying the course accordingly. The effect of the modifications on participant evaluation was reflected by substantially improved satisfaction scores at the second assessment in 14 of 16 items, with a significant increase in overall satisfaction from 3.6 ± 0.7 to 4.0 ± 0.4 (p = 0.008). CONCLUSION A combined quantitative and qualitative evaluation is a successful method for developing, evaluating, and improving a gerodontology course for dental students with high student satisfaction

Pharmacological activation of the ryanodine receptor in Jurkat T-lymphocytes

1. Recently, we provided evidence for cyclic adenosine 5′-diphosphate-ribose, cADP-ribose, as a second messenger in Jurkat T-lymphocytes upon stimulation of the T-cell receptor/CD3- complex (Guse et al., 1999). cADP-ribose mobilizes Ca(2+) from an intracellular Ca(2+) store which is sensitive to caffeine and gated by the ryanodine receptor/Ca(2+) release channel. In the present study we investigated the ability of the trypanocidal drug, suramin, to activate the ryanodine receptor of T-cells. Since suramin cannot permeate the plasma membrane, it was necessary to microinject the drug into Fura-2 loaded T-lymphocytes. 2. In a dose dependent manner suramin increased the intracellular Ca(2+) concentration. The dose-response curve is very steep and calculates for an EC(50) of 7.6±2.9 mM suramin in the injection pipette. 3. Co-injection of the selective ryanodine receptor inhibitor ruthenium red completely abolished the suramin induced Ca(2+) transient. This finding allows for the conclusion that the IP(3)-receptor sensitive Ca(2+) pool is not the primary target of the suramin induced Ca(2+) transient. 4. Furthermore, Ins(1,4,6)PS(3), an antagonist of the InsP(3)-receptor could not suppress the suramin-induced Ca(2+) signal. The suramin induced Ca(2+) transients declined very slowly; however, in the presence of Ins(1,4,6)PS(3) this decay was accelerated. In addition, suramin did not interact with the cADP-ribose binding site of the ryanodine receptor of T-cells. 5. In conclusion, suramin is found to be an agonist for the T-cell ryanodine receptor as previously found for the cardiac and skeletal muscle isoform. Therefore, suramin can be designated a universal ryanodine receptor agonist