Thermal equation of state of cubic boron nitride: Implications for a high-temperature pressure scale

The equation of state of cubic boron nitride (cBN) has been determined to a maximum temperature of 3300 K at a simultaneous static pressure of up to more than 70 GPa. Ab initio calculations to 80 GPa and 2000 K have also been performed. Our experimental data can be reconciled with theoretical results and with the known thermal expansion at 1 bar if we assume a small increase in pressure during heating relative to that measured at ambient temperature. The present data combined with the Raman measurements we presented earlier form the basis of a high-temperature pressure scale that is good to at least 3300 K

Phase boundary between Na–Si clathrates of structures I and II at high pressures and high temperatures

Understanding of the covalent clathrate formation is a crucial point for the design of new superhard materials with intrinsic coupling of superhardness and metallic conductivity. It has been found that silicon clathrates have the archetype structures, which can serve an existent model compounds for superhard clathrate frameworks Si–B, Si–C, B–C and C with intercalated atoms (e.g., alkali metals or even halogens) that can assure the metallic properties. Here we report our in situ and ex situ studies of high-pressure formation and stability of clathrates Na₈Si₄₆ (structure I) and Na₂₄₊xSi₁₃₆ (structure II). Experiments have been performed using standard Paris–Edinburgh cells (opposite anvils) up to 6 GPa and 1500 K. We have established that chemical interactions in the Na–Si system and transition between two structures of clathrates occur at temperatures below silicon melting. The strong sensitivity of crystallization products to the sodium concentration has been observed. A tentative diagram of clathrate transformations has been proposed. At least up to ~ 6 GPa, Na₂₄₊xSi₁₃₆ (structure II) is stable at lower temperatures as compared to Na₈Si₄₆ (structure I).Изучен in situ и ex situ процесс образования при высоких давлениях и стабильности клатратов Na₈Si₄₆ (структура I) и Na₂₄₊xSi₁₃₆ (структура II). Эксперименты были проведены в стандартных Париж-Эдинбургских ячейках (opposite anvils) при давлениях и температурах до 6 ГПа и 1500 K соответственно. Установлено, что химическое взаимодействие в системе Na–Si и переходы между двумя структурами клатратов происходят при температурах ниже температуры плавления кремния. Предложено первое приближение диаграммы превращений клатратов. Отмечена большая чувствительность продуктов кристаллизации к концентрации натрия. Na₂₄₊xSi₁₃₆ (структура II) является стабильной при более низких температурах по сравнению с Na₈Si₄₆ (структура I), по крайней мере до ~ 6 ГПа.Вивчено in situ і ex situ процес утворення при високих тисках і стабільності клатратів Na₈Si₄₆ (структура I) і Na₂₄₊xSi₁₃₆ (структура II). Експерименти було проведено в стандартних Париж-Единбурзький комірках (opposite anvils) при тисках і температурах до 6 ГПа і 1500 K відповідно. Встановлено, що хімічна взаємодія в системі Na–Si і переходи між двома структурами клатратов відбуваються при температурах нижче температури плавлення кремнію. Запропоновано перше наближення діаграми перетворень клатратів. Відзначено велику чутливість продуктів кристалізації до концентрації натрію. Na₂₄₊xSi₁₃₆ (структура II) є стабільною при більш низьких температурах у порівнянні з Na₈Si₄₆ (структура I), принаймні до ~ 6 ГПа

Attaching and effacing (A/E) lesion formation by enteropathogenic E. coli on human intestinal mucosa is dependent on non-LEE effectors

Enteropathogenic E. coli (EPEC) is a human pathogen that causes acute and chronic pediatric diarrhea. The hallmark of EPEC infection is the formation of attaching and effacing (A/E) lesions in the intestinal epithelium. Formation of A/E lesions is mediated by genes located on the pathogenicity island locus of enterocyte effacement (LEE), which encode the adhesin intimin, a type III secretion system (T3SS) and six effectors, including the essential translocated intimin receptor (Tir). Seventeen additional effectors are encoded by genes located outside the LEE, in insertion elements and prophages. Here, using a stepwise approach, we generated an EPEC mutant lacking the entire effector genes (EPEC0) and intermediate mutants. We show that EPEC0 contains a functional T3SS. An EPEC mutant expressing intimin but lacking all the LEE effectors but Tir (EPEC1) was able to trigger robust actin polymerization in HeLa cells and mucin-producing intestinal LS174T cells. However, EPEC1 was unable to form A/E lesions on human intestinal in vitro organ cultures (IVOC). Screening the intermediate mutants for genes involved in A/E lesion formation on IVOC revealed that strains lacking non-LEE effector/s have a marginal ability to form A/E lesions. Furthermore, we found that Efa1/LifA proteins are important for A/E lesion formation efficiency in EPEC strains lacking multiple effectors. Taken together, these results demonstrate the intricate relationships between T3SS effectors and the essential role non-LEE effectors play in A/E lesion formation on mucosal surfaces

The Moraxella adhesin UspA1 binds to its human CEACAM1 receptor by a deformable trimeric coiled-coil

Moraxella catarrhalis is a ubiquitous human-specific bacterium commonly associated with upper and lower respiratory tract infections, including otitis media, sinusitis and chronic obstructive pulmonary disease. The bacterium uses an autotransporter protein UspA1 to target an important human cellular receptor carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1). Using X-ray crystallography, we show that the CEACAM1 receptor-binding region of UspA1 unusually consists of an extended, rod-like left-handed trimeric coiled-coil. Mutagenesis and binding studies of UspA1 and the N-domain of CEACAM1 have been used to delineate the interacting surfaces between ligand and receptor and guide assembly of the complex. However, solution scattering, molecular modelling and electron microscopy analyses all indicate that significant bending of the UspA1 coiled-coil stalk also occurs. This explains how UspA1 can engage CEACAM1 at a site far distant from its head group, permitting closer proximity of the respective cell surfaces during infection

Modulation of host cell processes by T3SS effectors

Two of the enteric Escherichia coli pathotypes-enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC)-have a conserved type 3 secretion system which is essential for virulence. The T3SS is used to translocate between 25 and 50 bacterial proteins directly into the host cytosol where they manipulate a variety of host cell processes to establish a successful infection. In this chapter, we discuss effectors from EPEC/EHEC in the context of the host proteins and processes that they target-the actin cytoskeleton, small guanosine triphosphatases and innate immune signalling pathways that regulate inflammation and cell death. Many of these translocated proteins have been extensively characterised, which has helped obtain insights into the mechanisms of pathogenesis of these bacteria and also understand the host pathways they target in more detail. With increasing knowledge of the positive and negative regulation of host signalling pathways by different effectors, a future challenge is to investigate how the specific effector repertoire of each strain cooperates over the course of an infection

Expression of the Salmonella Spp. Virulence Factor SifA in Yeast Alters Rho1 Activity on Peroxisomes

SifA is a virulence protein required for assembly and tubulation of a modified phagosome that promotes Salmonella replication. We show that SifA expressed in yeast induces membrane invagination during peroxisome proliferation and requires functional Rho1p. This is consistent with SifA ability to interact with RhoA and the fact that it is a GEF structural homologue

Synchrotron x-ray computed microtomography for high pressure science

X-ray computed microtomography (XCT) has been a very promising and exciting technique for high pressure (HP) science since the introduction of the first HP setups optimized for tomography in the mid-2000s. Different experimental stations are now available using diamond anvil cells (DACs) or large volume presses, with their own benefits and limitations: access to very high pressures but at room temperature on one hand, high temperature (HT) at moderate pressures on the other, and slow acquisitions being an undesired common point between all techniques. We believe that we are at a turning point where current and future developments boost the interest of the technique for the HP community. Time-resolved experiments, with less than 1 s per tomogram, will become routinely available. Fast tomography will greatly reduce the problem of motion artifacts at HT, allowing new topics to be explored. Computing and data treatment issues must be taken into account to effectively exploit the large volumes of data produced. Foreseeable developments will allow higher pressures to be reached in larger volume presses and higher T in DACs. Furthermore, improved XCT resolution in large samples (several hundreds of μm in diameter) recorded in situ will offer to be an effective alternative to ex situ microscopy

Equation of state and post-stishovite transformation of Al-bearing silica up to 100 Gpa and 3000 K

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Compressibility of cubic vanadium mononitride

Abstract – The equation of state of vanadium mononitride with a cubic rock-salt structure, δ-VN, was measured on quasi-hydrostatic compression in a diamond anvil cell to 29GPa. From these data the bulk modulus of δ-VN, B0 = 300(6)GPa, and its first pressure derivative, B � 0 =3.6(6), were obtained. A comparison of the available data on B0 for δ-TiN, δ-ZrN, δ-HfN and δ-VN, extended by the present result, indicates a systematic agreement between the experimental B0-values from compression measurements and theoretical predictions based on the generalized gradient approximation (GGA) method. An increase of B0 with decreasing zero-pressure specific volume, earlier suggested for the group-4 and -5 transition metal mononitrides, is not confirmed. Copyright c� EPLA, 201