Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition

A unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009-2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the European Nucleotide Archive (ENA, www.ebi.ac.uk/ena). The association of these metadata to the experimental procedures applied for their generation will help the scientific community to access these data and facilitate their analysis. This paper complements other efforts to provide a full description of experiments and open science resources generated from the Tara Oceans project, further extending their value for the study of the world's planktonic ecosystems

Altimetry for the future: Building on 25 years of progress

In 2018 we celebrated 25 years of development of radar altimetry, and the progress achieved by this methodology in the fields of global and coastal oceanography, hydrology, geodesy and cryospheric sciences. Many symbolic major events have celebrated these developments, e.g., in Venice, Italy, the 15th (2006) and 20th (2012) years of progress and more recently, in 2018, in Ponta Delgada, Portugal, 25 Years of Progress in Radar Altimetry. On this latter occasion it was decided to collect contributions of scientists, engineers and managers involved in the worldwide altimetry community to depict the state of altimetry and propose recommendations for the altimetry of the future. This paper summarizes contributions and recommendations that were collected and provides guidance for future mission design, research activities, and sustainable operational radar altimetry data exploitation. Recommendations provided are fundamental for optimizing further scientific and operational advances of oceanographic observations by altimetry, including requirements for spatial and temporal resolution of altimetric measurements, their accuracy and continuity. There are also new challenges and new openings mentioned in the paper that are particularly crucial for observations at higher latitudes, for coastal oceanography, for cryospheric studies and for hydrology. The paper starts with a general introduction followed by a section on Earth System Science including Ocean Dynamics, Sea Level, the Coastal Ocean, Hydrology, the Cryosphere and Polar Oceans and the ‘‘Green” Ocean, extending the frontier from biogeochemistry to marine ecology. Applications are described in a subsequent section, which covers Operational Oceanography, Weather, Hurricane Wave and Wind Forecasting, Climate projection. Instruments’ development and satellite missions’ evolutions are described in a fourth section. A fifth section covers the key observations that altimeters provide and their potential complements, from other Earth observation measurements to in situ data. Section 6 identifies the data and methods and provides some accuracy and resolution requirements for the wet tropospheric correction, the orbit and other geodetic requirements, the Mean Sea Surface, Geoid and Mean Dynamic Topography, Calibration and Validation, data accuracy, data access and handling (including the DUACS system). Section 7 brings a transversal view on scales, integration, artificial intelligence, and capacity building (education and training). Section 8 reviews the programmatic issues followed by a conclusion

Altimetry for the future: building on 25 years of progress

In 2018 we celebrated 25 years of development of radar altimetry, and the progress achieved by this methodology in the fields of global and coastal oceanography, hydrology, geodesy and cryospheric sciences. Many symbolic major events have celebrated these developments, e.g., in Venice, Italy, the 15th (2006) and 20th (2012) years of progress and more recently, in 2018, in Ponta Delgada, Portugal, 25 Years of Progress in Radar Altimetry. On this latter occasion it was decided to collect contributions of scientists, engineers and managers involved in the worldwide altimetry community to depict the state of altimetry and propose recommendations for the altimetry of the future. This paper summarizes contributions and recommendations that were collected and provides guidance for future mission design, research activities, and sustainable operational radar altimetry data exploitation. Recommendations provided are fundamental for optimizing further scientific and operational advances of oceanographic observations by altimetry, including requirements for spatial and temporal resolution of altimetric measurements, their accuracy and continuity. There are also new challenges and new openings mentioned in the paper that are particularly crucial for observations at higher latitudes, for coastal oceanography, for cryospheric studies and for hydrology. The paper starts with a general introduction followed by a section on Earth System Science including Ocean Dynamics, Sea Level, the Coastal Ocean, Hydrology, the Cryosphere and Polar Oceans and the “Green” Ocean, extending the frontier from biogeochemistry to marine ecology. Applications are described in a subsequent section, which covers Operational Oceanography, Weather, Hurricane Wave and Wind Forecasting, Climate projection. Instruments’ development and satellite missions’ evolutions are described in a fourth section. A fifth section covers the key observations that altimeters provide and their potential complements, from other Earth observation measurements to in situ data. Section 6 identifies the data and methods and provides some accuracy and resolution requirements for the wet tropospheric correction, the orbit and other geodetic requirements, the Mean Sea Surface, Geoid and Mean Dynamic Topography, Calibration and Validation, data accuracy, data access and handling (including the DUACS system). Section 7 brings a transversal view on scales, integration, artificial intelligence, and capacity building (education and training). Section 8 reviews the programmatic issues followed by a conclusion

Sécurisation de la prise en charge du traitement personnel des patients hospitalisés (évaluation conduite à l'Hôpital Cochin-Saint Vincent de Paul)

PARIS-BIUP (751062107) / SudocSudocFranceF

Gestion des interactions médicamenteuses et des effets indésirables associés aux nouveaux antiviraux de l'hépatite C (étude menée à l'hôpital Cochin)

PARIS-BIUP (751062107) / SudocSudocFranceF

Bilan sur le sepsis sévère et le choc septique

Le choc septique se défini par une réponse inflammatoire liée à une infection, compliquée d'une hypotension persistante malgré un remplissage vasculaire ou nécessitant l'administration de catécholamine. La prise en charge du traitement comprend deux aspects : le traitement étiologique et le traitement symptomatique. Le choc septique présente un taux de mortalité entre 40 et 60%, constituant la première cause de décès en unité de soins intensifs. Les germes responsables du chos septique sont en constante évolution. L'épidémiologie très diverse conduit à des sémiologie polymorphes en fonction des susceptibilités cliniques et génétiques de chacun. Les recherches de traitements du choc septique ont abouti à de nombreux échecs. Ceux-ci sont partiellement expliqués par les imprécisions des définitions utilisées et la remise en cause des méthodes d'études. Le seul médicaments innovant du choc septique est la drotrégogine alpha activée, commercialisée sous le nom de XIGRIS. Adjuvant du traitement conventionnel, elle pallie le déficit en protéine C activée lors du choc septique, réduisant de 19,4% le taux de mortalité. La perspective d'avenir du traitement du choc septique est représentée par la puce à ADN, technologie permettant de connaître les susceptibilités de chaque patient afin d'adapter le traitement.CHATENAY M.-PARIS 11-BU Pharma. (920192101) / SudocSudocFranceF

Etude d'une cohorte de patients alcooliques en hospitalisation libre au centre hospitalier spécialisé psychiatrique de Rennes au cours de l'année 2000 (comparaison à un groupe de patients hospitalisés sans leur consentement)

RENNES1-BU Santé (352382103) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Des essais cliniques à l'A.M.M (impact financier d'une nouvelle génération de médicaments, les anticorps monoclonaux)

PARIS-BIUP (751062107) / SudocSudocFranceF

Propriétés interfaciales et émulsifiantes de la phosvitine du jaune d'oeuf (impacts de la charge et de l'agrégation)

La phosvitine du jaune d œuf est une protéine exceptionnellement riche en phosphosérines ce qui lui confère de grandes capacités de fixation des cations. Son caractère polyélectrolytique et sa faible hydrophobie limitent son adsorption aux interfaces air-eau et huile-eau mais à l inverse permettent une bonne stabilité des émulsions. Nous avons voulu comprendre l impact de sa charge, par ajout de NaCl, et de son état d agrégation, par ajout de calcium, sur ses capacités d adsorption aux interfaces en relation avec la stabilité des émulsions huile/eau. La diminution de charge permet un meilleur étalement de la phosvitine aux interfaces mais induit une déstabilisation plus rapide des émulsions. Les agrégats phosvitine-calcium n ont pas le même comportement à l interface en absence ou en présence de calcium libre. Dans ce dernier cas, le film interfacial créé est très structuré et rigide. Les conséquences sur la stabilité des émulsions dépendent ainsi de la séquence d addition du calciumHen egg yolk phosvitin is a protein exceptionally rich in phosphoserin residues. This confers to the protein a polyelectrolytic character and a high capacity to bind cations. The strong electrostatic charges and the low hydrophobicity of the phosvitin restrict its adsorption at the air-water and oil-water interfaces but allow a good emulsion stability. In this thesis, we wanted to understand the charge impact, by NaCl addition, and the aggregation impact, by calcium addition, on the protein adsorption capacity in relation to the stability of oil/water emulsion. The decrease of the charges allows a better spreading of the phosvitin at the interfaces but induces a faster destabilization of the emulsions. Phosvitin-calcium aggregates have different interfacial behavior depending on free calcium concentration. In presence of calcium a very structured and rigid interfacial network is created. So, the impact on emulsion stability depends on the timing of calcium addition.NANTES-BU Sciences (441092104) / SudocSudocFranceF