Effect of Fiber Length on Carbon Nanotube-Induced Fibrogenesis

Given their extremely small size and light weight, carbon nanotubes (CNTs) can be readily inhaled by human lungs resulting in increased rates of pulmonary disorders, particularly fibrosis. Although the fibrogenic potential of CNTs is well established, there is a lack of consensus regarding the contribution of physicochemical attributes of CNTs on the underlying fibrotic outcome. We designed an experimentally validated in vitro fibroblast culture model aimed at investigating the effect of fiber length on single-walled CNT (SWCNT)-induced pulmonary fibrosis. The fibrogenic response to short and long SWCNTs was assessed via oxidative stress generation, collagen expression and transforming growth factor-beta (TGF-β) production as potential fibrosis biomarkers. Long SWCNTs were significantly more potent than short SWCNTs in terms of reactive oxygen species (ROS) response, collagen production and TGF-β release. Furthermore, our finding on the length-dependent in vitro fibrogenic response was validated by the in vivolung fibrosis outcome, thus supporting the predictive value of the in vitro model. Our results also demonstrated the key role of ROS in SWCNT-induced collagen expression and TGF-β activation, indicating the potential mechanisms of length-dependent SWCNT-induced fibrosis. Together, our study provides new evidence for the role of fiber length in SWCNT-induced lung fibrosis and offers a rapid cell-based assay for fibrogenicity testing of nanomaterials with the ability to predict pulmonary fibrogenic response in viv

Retrieving Oil-Water Mixture Ratios of Marine Oil Spills From L-Band SAR Imagery

International audienceRetrieving the oil-water mixture ratio ( ${M}$ , the proportion of oil in an oil-water mixture) of the marine oil spill is of vital importance for the emergency treatment of oil pollution, which can be achieved by a copolarization ratio (PR) method. In the framework of the PR method, it is assumed that Bragg scattering dominates at moderate incidence angles, and the relative contribution of non-Bragg scattering to total scattering (RCNT) is typically neglected. However, in this work, it is found that the RCNT cannot be neglected for L-band, especially for HH polarization at higher wind speeds (the RCNT is approximately 0.6-0.8 for wind speeds of 10-12 m/s). The significant impact of non-Bragg scattering results in the PR method not being accurate enough for retrieving ${M}$. After separately investigating the influences of the damping effect and effective dielectric constant reduction on the RCNT, a novel approach is proposed for more accurately estimating the ${M}$ of marine oil spills by eliminating the impact of non-Bragg scattering. The proposed method was applied to uninhabited aerial vehicle synthetic aperture radar (UAVSAR) images collected during the Deepwater Horizon (DWH) oil spill accident. The inversion results of our approach show that the ${M}$ of most oil spill areas ranges from approximately 0.3 to 0.6, which matches the reality better than that of a traditional PR method (ranging from approximately 0.6 to 0.8)

Lunar Terrestrial Analog Experiment on the Spectral Interpretations of Rocks Observed by the Yutu-2 Rover

A visible and near-infrared imaging spectrometer (VNIS) loaded by the Chang’e-4 rover is the primary method for detecting the mineral composition of the lunar surface in the landing region. However, different data processing methods yield inconsistent mineral modes in measured lunar soil and rocks. To better constrain the mineral modes of the soil and rocks measured by Chang’e-4 VNIS, a noritic-gabbroic rock with a mineral composition similar to that of the lunar highland rocks is measured by scanning electron microscopy (SEM), the spare flight model of Chang’e-4 VNIS and TerraSpec-4 of ASD. Backscattered electron and energy dispersive spectrometry show that olivine, pyroxene, and plagioclase modal mineral abundances are 12.9, 35.0, and 52.2%, respectively. The estimated results of the spectrum by the Hapke radiative transfer model are 7.5, 39.3, and 53.2% for olivine, pyroxene, and plagioclase, respectively, which is consistent with to those of SEM mapping within error. In contrast, the estimated results of the modified Gaussian model are 29 and 71% for olivine and pyroxene, respectively, indicating the absence of plagioclase. Based on our implemented Hapke model, we decode the data of the two rocks detected by the rover on the 3rd and 26th lunar days of mission operations. The obtained results suggest that both rocks are norite or gabbro with noticeable differences. The first rock, with more olivine and pyroxene, may have been excavated from the Finsen crater. The second rock, with more plagioclase, may have been ejected from the southwestern edge of the Von Kármán crater, indicating the initial lunar crust

Efficient Biotransformation of Polysialogangliosides for Preparation of GM1 by Cellulosimicrobium sp. 21

A new ganglioside transformed strain isolated from soil was identified as Cellulosimicrobium sp. 21. It produced a sialidase which transformed polysialo-gangliosides GD1 and GT1 into a monosialoterahexosylganglioside, i.e., ganglioside GM1. The sialidase had both NeuAc-α-2,3- and NeuAc-α-2,8-sialidase activity without producing asiolo-GM1. The optimum conditions were evaluated and it was found that the transformation was optimally performed at 30 °C and pH 7.0. The substrate should be added at the beginning of the reaction and the concentration of substrate was 3% (w/v). Under these optimum conditions, Cellulosimicrobium sp. 21 converted GD1 and GT1 into GM1 in inorganic medium in a 5 L bioreactor with the recovery rate of 69.3%. The product contained 50.3% GM1 and was purified on silica to give the product with 95% of GM1 with a recovery rate of 30.5%. Therefore, Cellulosimicrobium sp. 21 has potential to be applied in the production of GM1 in the pharmaceutical industry

QTL for yield per plant under water deficit and well-watered conditions and drought susceptibility index in soybean (Glycine max (L.) Merr.)

AbstractDrought has historically represented the greatest abiotic stress to adversely affect the stability of soybean (Glycine max (L.) Merr.) yields in non-irrigated field conditions. To investigate the genetic basis underlying drought tolerance in soybean, we screened the seed yield per plant (YP) in a natural diversity panel of 149 accessions under both water deficit (drought-stressed; YP-S) and well-watered (full irrigation; YP-W) conditions in field and greenhouse trials and calculated a drought susceptibility index (DSI) based on the differences in yield between treatments within each accession. A total of 19 quantitative trait loci (QTLs) were identified: eight were significantly associated with YP-W, 10 were associated with YP-S, 2 were associated with DSI and one QTL was associated with both YP-S and DSI. We then compared QTLs identified here with previously reported markers and found that these loci were located in regions associated with yield-related and other agronomic traits. In particular, YP-S-associated single nucleotide polymorphism (SNP) ss246509299 in chromosome (Chr.) 8, and YP-W-associated ss249310678 on Chr. 17 were both previously correlated with canopy wilt. Eight significant QTLs were located within eight genes. Glyma.18g092200, contained ss249600231 and annotated as GmWRKY168, is reportedly responsive to cadmium. This study helps to resolve which loci contribute to yield under drought stress in soybean, and can potentially serve as markers for selection of elite, high-yield and drought tolerant varieties

CEPC Technical Design Report -- Accelerator

International audienceThe Circular Electron Positron Collider (CEPC) is a large scientific project initiated and hosted by China, fostered through extensive collaboration with international partners. The complex comprises four accelerators: a 30 GeV Linac, a 1.1 GeV Damping Ring, a Booster capable of achieving energies up to 180 GeV, and a Collider operating at varying energy modes (Z, W, H, and ttbar). The Linac and Damping Ring are situated on the surface, while the Booster and Collider are housed in a 100 km circumference underground tunnel, strategically accommodating future expansion with provisions for a Super Proton Proton Collider (SPPC). The CEPC primarily serves as a Higgs factory. In its baseline design with synchrotron radiation (SR) power of 30 MW per beam, it can achieve a luminosity of 5e34 /cm^2/s^1, resulting in an integrated luminosity of 13 /ab for two interaction points over a decade, producing 2.6 million Higgs bosons. Increasing the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons, facilitating precise measurements of Higgs coupling at sub-percent levels, exceeding the precision expected from the HL-LHC by an order of magnitude. This Technical Design Report (TDR) follows the Preliminary Conceptual Design Report (Pre-CDR, 2015) and the Conceptual Design Report (CDR, 2018), comprehensively detailing the machine's layout and performance, physical design and analysis, technical systems design, R&D and prototyping efforts, and associated civil engineering aspects. Additionally, it includes a cost estimate and a preliminary construction timeline, establishing a framework for forthcoming engineering design phase and site selection procedures. Construction is anticipated to begin around 2027-2028, pending government approval, with an estimated duration of 8 years. The commencement of experiments could potentially initiate in the mid-2030s

CEPC Technical Design Report -- Accelerator

International audienceThe Circular Electron Positron Collider (CEPC) is a large scientific project initiated and hosted by China, fostered through extensive collaboration with international partners. The complex comprises four accelerators: a 30 GeV Linac, a 1.1 GeV Damping Ring, a Booster capable of achieving energies up to 180 GeV, and a Collider operating at varying energy modes (Z, W, H, and ttbar). The Linac and Damping Ring are situated on the surface, while the Booster and Collider are housed in a 100 km circumference underground tunnel, strategically accommodating future expansion with provisions for a Super Proton Proton Collider (SPPC). The CEPC primarily serves as a Higgs factory. In its baseline design with synchrotron radiation (SR) power of 30 MW per beam, it can achieve a luminosity of 5e34 /cm^2/s^1, resulting in an integrated luminosity of 13 /ab for two interaction points over a decade, producing 2.6 million Higgs bosons. Increasing the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons, facilitating precise measurements of Higgs coupling at sub-percent levels, exceeding the precision expected from the HL-LHC by an order of magnitude. This Technical Design Report (TDR) follows the Preliminary Conceptual Design Report (Pre-CDR, 2015) and the Conceptual Design Report (CDR, 2018), comprehensively detailing the machine's layout and performance, physical design and analysis, technical systems design, R&D and prototyping efforts, and associated civil engineering aspects. Additionally, it includes a cost estimate and a preliminary construction timeline, establishing a framework for forthcoming engineering design phase and site selection procedures. Construction is anticipated to begin around 2027-2028, pending government approval, with an estimated duration of 8 years. The commencement of experiments could potentially initiate in the mid-2030s