The timing and magnitude of upper body muscular activity during a field hockey hit

The aim of this study was to investigate the contributions to stick motion in the field hockey hit by monitoring muscle activity in the arms and trunk and synchronising these with arm and stick kinematics. The hits of ten male, university-level field hockey players were analysed. Whilst their interpretation is complicated by the closed kinetic loop formed by the arms and stick, the data collected here represent a step forward in establishing the contributions from muscular activity and segmental interactions to the field hockey hit. This study has shown that EMG analysis alone is not sufficient to explain the nature of muscular activity patterns and that the temporal aspects of EMG need to be examined in combination with kinematic data to ascertain the role of muscular activity during movement

The timing and magnitude of muscular activity patterns during a field hockey hit

The field hockey hit is one of the most important skills used in the game. However, due to the paucity of empirical research, little is known about the biomechanics of this movement. Muscular activation patterns have been shown to be major contributing factors to performance variables in similar swinging motions in golf, tennis and baseball but debate remains about which muscles are contributing to and controlling such motions. Moreover, muscle studies have typically neglected the contribution to movement from segmental interactions and have not related muscle activity to the three-dimensional kinematics of the swing. The aim of this study was to investigate the contributions from muscular activity and from segmental interactions to the hits of ten male, university-level field hockey players. The activity of sixteen upper body and trunk muscles was monitored using surface electromyography alongside synchronized three-dimensional kinematics of the upper body and hockey stick motions. Surface electromyographic signals were recorded at 2000Hz bilaterally from the biceps brachii, triceps brachii, the anterior and posterior deltoids, the upper trapezius, the latissimus dorsi and the sternal and clavicular pectoralis major muscles. Three-dimensional kinematic data were collected at 240Hz and each hockey hit was broken down into four phases of the backswing, the early forward swing, acceleration and the early follow-through. These kinematic and electromyographic data were then synchronised and temporally normalised before the electromyographic data were normalised to relative maximal reference contractions. Right anterior deltoid, right pectoralis major and bilateral latissimus dorsi activity initiates the downswing of the hockey hit, causing the early acceleration of the arms. Segmental interactions, due to these accelerations, cause the hockey stick to lag and the wrists to ‘cock’. A combination of left anterior deltoid, left latissimus dorsi and bilateral pectoralis major activity continue to accelerate the shoulders during the downswing whilst elbow musculature appears to control the effects of segmental interactions. These segmental interactions then become involved in wrist ‘uncocking’ as the stick accelerates towards impact with the ball. The effects of muscular activity and segmental interactions cause the right elbow to flex then extend, whereas the left elbow demonstrates a more constant degree of extension throughout the hit. Both wrists display the same pattern of ‘cocking’ then ‘uncocking’. These combined patterns lead the left arm and stick system to function as a double pendulum whilst the right arm and stick more closely resemble a triple pendulum

Vibration analysis of a circular plate in interaction with an acoustic cavity leading to extraction of structural modal parameters

When carrying out vibration health monitoring (VHM) of a structure it is usually assumed that the structure is in the absence of fluid interaction and that any environmental effects which can cause changes in natural frequency either remain constant or are negligible. In certain cases, this condition cannot be assumed and therefore it is necessary to extract values of natural frequencies of the structure for the condition with no fluid interaction from those values measured. This paper considers the case of a thin circular plate in contact with a fluid cavity giving rise to strong structural/fluid vibration interaction. The paper details the free vibration analysis of the coupled system and through consideration of modal energy, illustrates how the affined modes of vibration of the plate and the fluid can be qualitatively described. The paper then introduces a method by which the natural frequencies of the plate in the absence of fluid interaction can be obtained from those of the plate in interaction with the fluid

THE EFFECTS OF CONCURRENT BIOFEEDBACK ON ROWING PERFORMANCE

The aim of this study was to assess the effects of concurrent biofeedback (BFb) on the ability of skilled rowers to modify the relative motions of their elbow and knee joints during ergometer rowing. Over 2 weeks, BFb (n=7) and control (n=7) participants completed two maximal rowing tasks (pre-intervention; transfer) separated by three submaximal rowing sessions supplemented with BFb for the BFb group. Pre-intervention to transfer session patterns showed increased elbow extension and knee flexion in the early phases of the pull, which was a move towards the pattern advocated by the BFb intervention. Although these alterations were not universal, BFb appears to be a useful training aid for those further from the target movement pattern

EFFECTS OF NON-INSTRUCTED PRACTICE ON A NOVEL ROWING TASK

Information feedback has been shown to be an important part of the learning process, yet changes have not been assessed within sporting applications. Non-rowers (n=7) performed a 10-minute novel rowing task, and joint and rowing ergometer kinematics recorded. Following four non-instructed practice rowing sessions, their techniques were reassessed. Results showed that the ergometer handle trajectory became more elliptical throughout the stroke and that the knees were more flexed at catch (11°) and more extended at the finish (13°). Changes in the shape of the handle trajectory caused changes in the lengths of the pull and recovery phases and implied changes in the timing of joint motions. This study is a step towards understanding the motor learning of novices

THE EFFECTS OF AUGMENTED BIOFEEDBACK ON NOVEL MOTOR-TASK LEARNING

Biofeedback has been shown to be an influential part of skill acquisition and performance, however, the use of biofeedback for novice, sports specific skill learning has not been assessed. Non-rowers (n=3) performed a 10-minute, novel-rowing task, where joint and rowing ergometer kinematics recorded. Following six non-instructed, subjective reinforcement sessions, participants completed a further six sessions whilst receiving real-time biofeedback. The results show that all subjects changed their rowing technique, moving towards the pattern prescribed by the biofeedback intervention. The elbow remained in greater extension until later in the pull, which induced changes in the temporal aspects of both knee and lumbar spine kinematics

A Naturally Selected Dimorphism within the HLA-B44 Supertype Alters Class I Structure, Peptide Repertoire, and T Cell Recognition

HLA-B*4402 and B*4403 are naturally occurring MHC class I alleles that are both found at a high frequency in all human populations, and yet they only differ by one residue on the α2 helix (B*4402 Asp156→B*4403 Leu156). CTLs discriminate between HLA-B*4402 and B*4403, and these allotypes stimulate strong mutual allogeneic responses reflecting their known barrier to hemopoeitic stem cell transplantation. Although HLA-B*4402 and B*4403 share >95% of their peptide repertoire, B*4403 presents more unique peptides than B*4402, consistent with the stronger T cell alloreactivity observed toward B*4403 compared with B*4402. Crystal structures of B*4402 and B*4403 show how the polymorphism at position 156 is completely buried and yet alters both the peptide and the heavy chain conformation, relaxing ligand selection by B*4403 compared with B*4402. Thus, the polymorphism between HLA-B*4402 and B*4403 modifies both peptide repertoire and T cell recognition, and is reflected in the paradoxically powerful alloreactivity that occurs across this “minimal” mismatch. The findings suggest that these closely related class I genes are maintained in diverse human populations through their differential impact on the selection of peptide ligands and the T cell repertoire

Fine-mapping of the HNF1B multicancer locus identifies candidate variants that mediate endometrial cancer risk.

Common variants in the hepatocyte nuclear factor 1 homeobox B (HNF1B) gene are associated with the risk of Type II diabetes and multiple cancers. Evidence to date indicates that cancer risk may be mediated via genetic or epigenetic effects on HNF1B gene expression. We previously found single-nucleotide polymorphisms (SNPs) at the HNF1B locus to be associated with endometrial cancer, and now report extensive fine-mapping and in silico and laboratory analyses of this locus. Analysis of 1184 genotyped and imputed SNPs in 6608 Caucasian cases and 37 925 controls, and 895 Asian cases and 1968 controls, revealed the best signal of association for SNP rs11263763 (P = 8.4 × 10(-14), odds ratio = 0.86, 95% confidence interval = 0.82-0.89), located within HNF1B intron 1. Haplotype analysis and conditional analyses provide no evidence of further independent endometrial cancer risk variants at this locus. SNP rs11263763 genotype was associated with HNF1B mRNA expression but not with HNF1B methylation in endometrial tumor samples from The Cancer Genome Atlas. Genetic analyses prioritized rs11263763 and four other SNPs in high-to-moderate linkage disequilibrium as the most likely causal SNPs. Three of these SNPs map to the extended HNF1B promoter based on chromatin marks extending from the minimal promoter region. Reporter assays demonstrated that this extended region reduces activity in combination with the minimal HNF1B promoter, and that the minor alleles of rs11263763 or rs8064454 are associated with decreased HNF1B promoter activity. Our findings provide evidence for a single signal associated with endometrial cancer risk at the HNF1B locus, and that risk is likely mediated via altered HNF1B gene expression

Repair of Acute Respiratory Distress Syndrome in COVID-19 by Stromal Cells (REALIST-COVID Trial):A Multicentre, Randomised, Controlled Trial

RationaleMesenchymal stromal cells (MSCs) may modulate inflammation, promoting repair in COVID-19-related Acute Respiratory Distress Syndrome (ARDS).ObjectivesWe investigated safety and efficacy of ORBCEL-C (CD362-enriched, umbilical cord-derived MSCs) in COVID-related ARDS.MethodsThis multicentre, randomised, double-blind, allocation concealed, placebo-controlled trial (NCT03042143) randomised patients with moderate-to-severe COVID-related ARDS to receive ORBCEL-C (400million cells) or placebo (Plasma-Lyte148).MeasurementsThe primary safety and efficacy outcomes were incidence of serious adverse events and oxygenation index at day 7 respectively. Secondary outcomes included respiratory compliance, driving pressure, PaO2/FiO2 ratio and SOFA score. Clinical outcomes relating to duration of ventilation, length of intensive care unit and hospital stays, and mortality were collected. Long-term follow up included diagnosis of interstitial lung disease at 1 year, and significant medical events and mortality at 2 years. Transcriptomic analysis was performed on whole blood at day 0, 4 and 7.Main results60 participants were recruited (final analysis n=30 ORBCEL-C, n=29 placebo: 1 in placebo group withdrew consent). 6 serious adverse events occurred in the ORBCEL-C and 3 in the placebo group, RR 2.9(0.6-13.2)p=0.25. Day 7 mean[SD] oxygenation index did not differ (ORBCEL-C 98.357.2], placebo 96.667.3). There were no differences in secondary surrogate outcomes, nor mortality at day 28, day 90, 1 or 2 years. There was no difference in prevalence of interstitial lung disease at 1year nor significant medical events up to 2 years. ORBCEL-C modulated the peripheral blood transcriptome.ConclusionORBCEL-C MSCs were safe in moderate-to-severe COVID-related ARDS, but did not improve surrogates of pulmonary organ dysfunction. Clinical trial registration available at www.Clinicaltrialsgov, ID: NCT03042143. This article is open access and distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/)