HIV and antiretroviral treatment knowledge gaps and psychosocial burden among persons living with HIV in Lima, Peru

This study aims to describe knowledge on HIV and antiretroviral (ARV) treatment and psychosocial factors among people living with HIV (PLWH) in Lima, Perú, to explore characteristics associated to this knowledge, and determine its impact on sustained viral suppression. A cross-sectional survey was conducted among 171 PLWH at the largest referral health care center in Lima. The psychosocial factors measured were depression, risk of alcoholism, use of illegal drugs and disclosure. A participant had "poor knowledge" when less than 80% of replies were correct. Sustained viral suppression was defined as two consecutive viral loads under 50 copies/mL. A total of 49% and 43% had poor HIV and ARV knowledge respectively; 48% of the study population screened positive for depression and 27% reported feeling unsupported by the person they disclosed to. The largest gaps in HIV and ARV knowledge were among 98 (57%) that did not recognize that HIV increased the risk of cancer and among 57 (33%) participants that did not disagree with the statement that taking a double dose of ARV if they missed one. Moderate depression was significantly associated to poor HIV and ARV knowledge. Non-disclosure and being on ARVs for less than 6 months were associated with not achieving sustained viral suppression. Our findings highlight important HIV and ARV knowledge gaps of PLWH and a high burden of psychosocial problems, especially of depression, among PLWH in Lima, Peru. Increasing knowledge and addressing depression and disclosure could improve care of PLWH

Clustering and descendants of MUSYC galaxies at z<1.5

We measure the evolution of galaxy clustering out to a redshift of z~1.5 using data from two MUSYC fields, the Extended Hubble Deep Field South (EHDF-S) and the Extended Chandra Deep Field South (ECDF-S). We use photometric redshift information to calculate the projected-angular correlation function, omega(sigma), from which we infer the projected correlation function Xi(sigma). We demonstrate that this technique delivers accurate measurements of clustering even when large redshift measurement errors affect the data. To this aim we use two mock MUSYC fields extracted from a LambdaCDM simulation populated with GALFORM semi-analytic galaxies which allow us to assess the degree of accuracy of our estimates of Xi(sigma) and to identify and correct for systematic effects in our measurements. We study the evolution of clustering for volume limited subsamples of galaxies selected using their photometric redshifts and rest-frame r-band absolute magnitudes. We find that the real-space correlation length r_0 of bright galaxies, M_r<-21 (rest-frame) can be accurately recovered out to z~1.5, particularly for ECDF-S given its near-infrared photometric coverage. There is mild evidence for a luminosity dependent clustering in both fields at the low redshift samples (up to =0.57), where the correlation length is higher for brighter galaxies by up to 1Mpc/h between median rest-frame r-band absolute magnitudes of -18 to -21.5. As a result of the photometric redshift measurement, each galaxy is assigned a best-fit template; we restrict to E and E+20%Sbc types to construct subsamples of early type galaxies (ETGs). Our ETG samples show a strong increase in r_0 as the redshift increases, making it unlikely (95% level) that ETGs at median redshift z_med=1.15 are the direct progenitors of ETGs at z_med=0.37 with equivalent passively evolved luminosities. (ABRIDGED)Comment: 16 pages, 12 figures, 2 tables, accepted for publication in MNRA

Genetic diversity of Phytophthora infestans in the Northern Andean region.

RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.BACKGROUND: Phytophthora infestans (Mont.) de Bary, the causal agent of potato late blight, is responsible for tremendous crop losses worldwide. Countries in the northern part of the Andes dedicate a large proportion of the highlands to the production of potato, and more recently, solanaceous fruits such as cape gooseberry (Physalis peruviana) and tree tomato (Solanum betaceum), all of which are hosts of this oomycete. In the Andean region, P. infestans populations have been well characterized in Ecuador and Peru, but are poorly understood in Colombia and Venezuela. To understand the P. infestans population structure in the Northern part of the Andes, four nuclear regions (ITS, Ras, β-tubulin and Avr3a) and one mitochondrial (Cox1) region were analyzed in isolates of P. infestans sampled from different hosts in Colombia and Venezuela. RESULTS: Low genetic diversity was found within this sample of P. infestans isolates from crops within several regions of Colombia and Venezuela, revealing the presence of clonal populations of the pathogen in this region. We detected low frequency heterozygotes, and their distribution patterns might be a consequence of a high migration rate among populations with poor effective gene flow. Consistent genetic differentiation exists among isolates from different regions. CONCLUSIONS: The results here suggest that in the Northern Andean region P. infestans is a clonal population with some within-clone variation. P. infestans populations in Venezuela reflect historic isolation that is being reinforced by a recent self-sufficiency of potato seeds. In summary, the P. infestans population is mainly shaped by migration and probably by the appearance of variants of key effectors such as Avr3a

Production of cadmium sulfide quantum dots by the lithobiontic Antarctic strain Pedobacter sp. UYP1 and their application as photosensitizer in solar cells

Indexación ScopusBackground: Microbes are present in almost every environment on Earth, even in those with extreme environmental conditions such as Antarctica, where rocks may represent the main refuge for life. Lithobiontic communities are composed of microorganisms capable of colonizing rocks and, as it is a not so well studied bacterial community, they may represent a very interesting source of diversity and functional traits with potential for biotechnological applications. In this work we analyzed the ability of Antarctic lithobiontic bacterium to synthesize cadmium sulfide quantum dots (CdS QDs) and their potential application in solar cells. Results: A basaltic andesite rock sample was collected from Fildes Peninsula, King George Island, Antarctica, and processed in order to isolate lithobiontic bacterial strains. Out of the 11 selected isolates, strain UYP1, identified as Pedobacter, was chosen for further characterization and analysis due to its high cadmium tolerance. A protocol for the biosynthesis of CdS QDs was developed and optimized for this strain. After 20 and 80 min of synthesis, yellow-green and orange-red fluorescent emissions were observed under UV light, respectively. QDs were characterized through spectroscopic techniques, dynamic light scattering analysis, high-resolution transmission electron microscopy and energy dispersive x-ray spectroscopy. Nanostructures of 3.07 nm, composed of 51.1% cadmium and 48.9% sulfide were obtained and further used as photosensitizer material in solar cells. These solar cells were able to conduct electrons and displayed an open circuit voltage of 162 mV, a short circuit current density of 0.0110 mA cm−2, and had an efficiency of conversion up to 0.0016%, which is comparable with data previously reported for solar cells sensitized with biologically produced quantum dots. Conclusions: We report a cheap, rapid and eco-friendly protocol for the production of CdS QDs by an Antarctic lithobiontic bacterium, Pedobacter, a genus that was not previously reported as a quantum dot producer. The application of the biosynthesized QDs as sensitizer material in solar cells was validated. © 2021, The Author(s).https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-021-01531-

Star Cluster Complexes and the Host Galaxy in Three HII Galaxies: Mrk 36, UM 408, and UM 461

We present a stellar population study of three HII galaxies (Mrk 36, UM 408, and UM 461) based on the analysis of new ground-based high resolution near-infrared J, H and Kp broad-band and Br narrow-band images obtained with Gemini/NIRI. We identify and determine relative ages and masses of the elementary star clusters and/or star cluster complexes of the starburst regions in each of these galaxies by comparing the colors with evolutionary synthesis models that include the contribution of stellar continuum, nebular continuum and emission lines. We found that the current star cluster formation efficiency in our sample of low luminosity HII galaxies is ~10%. Therefore, most of the recent star formation is not in massive clusters. Our findings seem to indicate that the star formation mode in our sample of galaxies is clumpy, and that these complexes are formed by a few massive star clusters with masses > 10^4 Mo. The age distribution of these star cluster complexes shows that the current burst started recently and likely simultaneously over short time scales in their host galaxies, triggered by some internal mechanism. Finally, the fraction of the total cluster mass with respect to the low surface brightness (or host galaxy) mass, considering our complete range in ages, is less than 1%.Comment: 39 pages, 9 figures, 4 tables. Accepted for publication in The Astronomical Journa

Identification and functional analysis of missense mutations in the lecithin cholesterol acyltransferase gene in a Chilean patient with hypoalphalipoproteinemia

Lecithin-cholesterol acyltransferase (LCAT) is a plasma enzyme that esterifies cholesterol in high- and low-density lipoproteins (HDL and LDL). Mutations in LCAT gene causes familial LCAT deficiency, which is characterized by very low plasma HDL-cholesterol levels (Hypoalphalipoproteinemia), corneal opacity and anemia, among other lipid-related traits. Our aim is to evaluate clinical/biochemical features of a Chilean family with a proband showing clinical signs of familial LCAT deficiency, as well as to identify and assess the functional effects of LCAT mutations. LCAT sequencing identified rare p.V333 M and p.M404 V missense mutations in compound heterozygous state in the proband, as well the common synonymous p.L363 L variant. LCAT protein was detected in proband’s plasma, but with undetectable enzyme activity compared to control relatives. HEK-293 T transfected cells with vector expression plasmids containing either p.M404 V or p.V333 M cDNA showed detectable LCAT protein expression both in supernatants and lysates from cultured cells, but with much lower enzyme activity compared to cells transfected with the wild-type sequence. Bioinformatic analyses also supported a causal role of such rare variations in LCAT lack of function. Additionally, the proband carried the minor allele of the synonymous p.L363 L variant. However, this variant is unlikely to affect the clinical phenotype of the proband given its relatively high frequency in the Chilean population (4%) and its small putative effect on plasma HDL-cholesterol levels. Conclusion: Genetic, biochemical, in vitro and in silico analyses indicate that the rare mutations p.M404 V and p. V333 M in LCAT gene lead to suppression of LCAT enzyme activity and cause clinical features of familial LCAT deficiency.This work was supported by Proyecto FONDECYT 1150416 and Proyecto Interdisciplina VRI-PUC II15024 from the Dirección de Investigación, Pontificia Universidad Católica de Chile. Genotyping of GOCS was performed in the in the Human Genotyping laboratory at the Spanish National Cancer Research Centre, a member of CeGen (PRB2-ISCIII), and was supported by grant PT13/ 0001/0005 of PE I + D + i 2013-2016 funded by ISCIII and ERDF (Fondo Europeo de Desarrollo Regional). This research was partially supported by the supercomputing infrastructure of the NLHPC (ECM-02). L.V. and C.B. were supported by VRI, Pontificia Universidad Católica de Chile (Proyecto Investigación Interdisciplinaria VRI-PUC II15024). TG was supported by “Beca de Magíster Nacional” CONICYT. L.V. was additionally supported by FONDECYT postdoctoral grant 3170038. We express our gratitude to the proband and relatives

Economic Valuation of Health Care Services in Public Health Systems: A Study about Willingness to Pay (WTP) for Nursing Consultations

Background: Identifying the economic value assigned by users to a particular health service is of principal interest in planning the service. The aim of this study was to evaluate the perception of economic value of nursing consultation in primary care (PC) by its users. Methods and Results: Economic study using contingent valuation methodology. A total of 662 users of nursing consultation from 23 health centers were included. Data on demographic and socioeconomic characteristics, health needs, pattern of usage, and satisfaction with provided service were compiled. The validity of the response was evaluated by an explanatory mixed-effects multilevel model in order to assess the factors associated with the response according to the welfare theory. Response reliability was also evaluated. Subjects included in the study indicated an average Willingness to Pay (WTP) of €14.4 (CI 95%: €13.2–15.5; median €10) and an average Willingness to Accept [Compensation] (WTA) of €20.9 (CI 95%: €19.6–22.2; median €20). Average area income, personal income, consultation duration, home visit, and education level correlated with greater WTP. Women and older subjects showed lower WTP. Fixed parameters explained 8.41% of the residual variability, and response clustering in different health centers explained 4–6% of the total variability. The influence of income on WTP was different in each center. The responses for WTP and WTA in a subgroup of subjects were consistent when reassessed after 2 weeks (intraclass correlation coefficients 0.952 and 0.893, respectively). Conclusions: The economic value of nursing services provided within PC in a public health system is clearly perceived by its user. The perception of this value is influenced by socioeconomic and demographic characteristics of the subjects and their environment, and by the unique characteristics of the evaluated service. The method of contingent valuation is useful for making explicit this perception of value of health services

GECO: Galaxy Evolution COde - A new semi-analytical model of galaxy formation

We present a new semi-analytical model of galaxy formation, GECO (Galaxy Evolution COde), aimed at a better understanding of when and how the two processes of star formation and galaxy assembly have taken place. Our model is structured into a Monte Carlo algorithm based on the Extended Press-Schechter theory, for the representation of the merging hierarchy of dark matter halos, and a set of analytic algorithms for the treatment of the baryonic physics, including classical recipes for the gas cooling, the star formation time-scales, galaxy mergers and SN feedback. Together with the galaxies, the parallel growth of BHs is followed in time and their feedback on the hosting galaxies is modelled. We set the model free parameters by matching with data on local stellar mass functions and the BH-bulge relation at z=0. Based on such local boundary conditions, we investigate how data on the high-redshift universe constrain our understanding of the physical processes driving the evolution, focusing in particular on the assembly of stellar mass and on the star formation history. Since both processes are currently strongly constrained by cosmological near- and far-IR surveys, the basic physics of the Lambda CDM hierarchical clustering concept of galaxy formation can be effectively tested by us by comparison with the most reliable set of observables. Our investigation shows that when the time-scales of the stellar formation and mass assembly are studied as a function of dark matter halo mass and the single galaxy stellar mass, the 'downsizing' fashion of star formation appears to be a natural outcome of the model, reproduced even in the absence of the AGN feedback. On the contrary, the stellar mass assembly history turns out to follow a more standard hierarchical pattern progressive in cosmic time, with the more massive systems assembled at late times mainly through dissipationless mergers.Comment: Accepted for publication in A&A, 24 pages, 15 figure

UFR2709, a nicotinic acetylcholine receptor antagonist, decreases ethanol intake in alcohol-preferring rats

Brain nicotinic acetylcholine receptors (nAChRs), a heterogeneous family of pentameric acetylcholine-gated cation channels, have been suggested as molecular targets for the treatment of alcohol abuse and dependence. Here, we examined the effect of the competitive nAChR antagonist UFR2709 on the alcohol consumption of high-alcohol-drinking UChB rats. UChB rats were given free access to ethanol for 24-h periods in a two-bottle free-choice paradigm and their ethanol and water intake were measured. The animals were i.p injected daily for 17 days with a 10, 5, 2.5 or 1 mg/kg dose of UFR2709. Potential confounding motor effects of UFR2709 were assessed by examining the locomotor activity of animals administered the highest dose of UR2709 tested (10 mg/kg i.p.). UFR2709 reduced ethanol consumption and ethanol preference and increased water consumption in a dose-dependent manner. The most effective dose of UFR2709 was 2.5 mg/kg, which induced a 56% reduction in alcohol consumption. Administration of UFR2709 did not affect the weight or locomotor activity of the rats, suggesting that its effects on alcohol consumption and preference were mediated by specific nAChRs

Adaptación cultural al español del instrumento de evaluación de funcionalidad física en Unidad de Paciente Crítico: “The Chelsea Critical Care Physical Assessment Tool (CPAx)”

Las Unidades de Cuidados Intensivos (UCI), presentan una sobrevida cada vez mayor de los pacientes que ingresan a ellas, donde se ven enfrentados a una nueva entidad fisiopatológica llamada Debilidad Muscular Adquirida en UCI (DAUCI). Algunos test desarrollados para la evaluación de función motriz, que permiten objetivar la progresión del paciente, son la escala de fuerza muscular del Medical Research Council (MRC), el Functional status score for the intensive care unit (FSS-ICU) y el “Chelsea Critical Care Physical Assessment Tool (CPAx). La ventaja del CPAx radica en que este test incluye mayor información asociada al funcionamiento humano como recomienda la OMS, incorporando tanto el componente ventilatorio (que también se ve deteriorado por DAUCI) como neuromuscular lo que permite al profesional kinesiólogo tener una herramienta objetiva más completa del nivel funcional del paciente. Para que sea confiable, todo test debe ser validado en el país donde quiere aplicarse, pero antes de esto debe ser adaptado culturalmente. El objetivo de este trabajo fue efectuar la adaptación transcultural (AT) al español del test de funcionalidad física de aplicación kinésica CPAx. Se utilizó el proceso establecido por Beaton y cols que incluye la formación de un comité de expertos multidisciplinario que da una visión integral a la adaptación y una prueba piloto en que kinesiólogos de UCI sin capacitación previa del test lo lean, posteriormente lo apliquen y entreguen sus observaciones. Conclusiones: Realizar la AT permite dimensionar la importancia que tiene cada una de las etapas de este proceso. El test es el mismo, equivalente al original, pero contiene nuestras características culturales y condiciones técnicas, que lo hace ser comprensible y aplicable en nuestro país. Esta adaptación transcultural también es útil a nivel latinoamericano; para los países de habla hispana que quieran validarlo tenerlo adaptado al español, hace el proceso menos complejo. Palabras clave: Evaluación funcional, Unidad de cuidados intensivos, CPAx, adaptación transcultural