Sarkocistoza papkarske divljači u Slovačkoj

In this paper, we analyze the results of surveys of Sarcocystis spp. carried out on heart and skeletal muscles from 71 ex. of cloven-hoofed game hunted in Central and Eastern Slovakia between 2005 and 2007. Sarcocystis infection was determined among 91.5 % of the 71 cloven-hoofed game animals (deer, mouflon, chamois, wild boar): 100% in 13 roe deer, 10 mouflons, 8 fallow deer, and 6 chamois; 85.0 % in 20 wild boars, 78.6 % in 14 red deer. In examined cloven-hoofed game in Slovakia a high intensity of infection of Sarcocystis spp., was recorded. The highest intensity of infection was in wild boars (up to 1,495 microcysts per gram) and roe deer (903 microcysts per gram). Animals under one year of age showed low intensity of infection in comparison with adults: red deer (19/g), mouflon (19/g), chamois (19/g) and wild boar (52/g). Muscle samples of males had a significantly higher intensity of infection than females. Intensity of infection was statistically significant in red deer male versus calves p<0.05; chamois male versus lamb p<0.001, chamois female versus lamb p<0.001, chamois male versus female p<0.05, wild boar male versus female p<0.01, wild boar female versus piglet p<0.05. We also present the morphology of thin-walled microcysts of Sarcocystis spp. according to histological sections. Light microscopy showed typical features of Sarcocystis spp. microcysts in skeletal muscles and myocardium. Thin-walled microcysts were filled with numerous bradyzoites, which are situated inside chamber-like hollows in the cysts.U radu je istraživana prisutnost parazita Sarcocystis spp. u poprečno-prugastom srčanom i skeletnom mišićju 71 primjerka papkarske divljači odstrijeljene u razdoblju od 2005. do 2007. na području središnje i istočne Slovačke. Invazija sarkocistama je utvrđena u 91% pregledane divljači (jelenska divljač, muflon, divokoza, divlja svinja), i to u 100% od pregledanih 13 srna, 10 muflona, 8 jelena lopatara i 6 divokoza; 85.0% u 20 pregledanih divljih svinja; 78.6% u pregledanih 14 jelena običnih. Također je u pretražene papkarske divljači zabilježen i visoki intenzitet invazije, najviši u divljih svinja (do 1.495 mikrocista po gramu) i srna (903 mikrociste po gramu). Niži intenzitet invazije zabilježen je u mladunčadi u odnosu na odrasle životinje; jelen obični (19/g), muflon (19/g), divokoza (19/g) i divlja svinja (52/g). Statistički značajno jača invazija utvrđena je u mužjaka negoli u ženki. Nadalje, statistički značajne razlike uočene su u intenzitetu invazije kod mužjaka jelena običnog u odnosu na telad p<0.05; divojaraca u odnosu na janjad p<0.001, divokoza u odnosu na janjad p<0.001, divojaraca u odnosu na divokoze p<0.05, veprova u odnosu na krmače p<0.01 te krmača u odnosu na prasad p<0.05. U radu su također prikazane i morfološke osobitosti mikrocista parazita Sarcocystis spp. u poprečno-prugastom skeletnom i srčanom mišićju, prema histološkom prikazu. Mikrociste su bile ispunjene brojnim bradizoitima, smještenima unutar komorica

Cryptosporidium parvum – zoonotic subtype IIdA15G1 in a Slovakian patient

Introduction and objectives. The parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. Materials and method. The Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes. Results. The nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified. Conclusion. This is the first confirmed case in Slovakia of human cryptosporidiosis caused by the unique subtype IIdA15G1

Sarkocistoza papkarske divljači u Slovačkoj

In this paper, we analyze the results of surveys of Sarcocystis spp. carried out on heart and skeletal muscles from 71 ex. of cloven-hoofed game hunted in Central and Eastern Slovakia between 2005 and 2007. Sarcocystis infection was determined among 91.5 % of the 71 cloven-hoofed game animals (deer, mouflon, chamois, wild boar): 100% in 13 roe deer, 10 mouflons, 8 fallow deer, and 6 chamois; 85.0 % in 20 wild boars, 78.6 % in 14 red deer. In examined cloven-hoofed game in Slovakia a high intensity of infection of Sarcocystis spp., was recorded. The highest intensity of infection was in wild boars (up to 1,495 microcysts per gram) and roe deer (903 microcysts per gram). Animals under one year of age showed low intensity of infection in comparison with adults: red deer (19/g), mouflon (19/g), chamois (19/g) and wild boar (52/g). Muscle samples of males had a significantly higher intensity of infection than females. Intensity of infection was statistically significant in red deer male versus calves p<0.05; chamois male versus lamb p<0.001, chamois female versus lamb p<0.001, chamois male versus female p<0.05, wild boar male versus female p<0.01, wild boar female versus piglet p<0.05. We also present the morphology of thin-walled microcysts of Sarcocystis spp. according to histological sections. Light microscopy showed typical features of Sarcocystis spp. microcysts in skeletal muscles and myocardium. Thin-walled microcysts were filled with numerous bradyzoites, which are situated inside chamber-like hollows in the cysts.U radu je istraživana prisutnost parazita Sarcocystis spp. u poprečno-prugastom srčanom i skeletnom mišićju 71 primjerka papkarske divljači odstrijeljene u razdoblju od 2005. do 2007. na području središnje i istočne Slovačke. Invazija sarkocistama je utvrđena u 91% pregledane divljači (jelenska divljač, muflon, divokoza, divlja svinja), i to u 100% od pregledanih 13 srna, 10 muflona, 8 jelena lopatara i 6 divokoza; 85.0% u 20 pregledanih divljih svinja; 78.6% u pregledanih 14 jelena običnih. Također je u pretražene papkarske divljači zabilježen i visoki intenzitet invazije, najviši u divljih svinja (do 1.495 mikrocista po gramu) i srna (903 mikrociste po gramu). Niži intenzitet invazije zabilježen je u mladunčadi u odnosu na odrasle životinje; jelen obični (19/g), muflon (19/g), divokoza (19/g) i divlja svinja (52/g). Statistički značajno jača invazija utvrđena je u mužjaka negoli u ženki. Nadalje, statistički značajne razlike uočene su u intenzitetu invazije kod mužjaka jelena običnog u odnosu na telad p<0.05; divojaraca u odnosu na janjad p<0.001, divokoza u odnosu na janjad p<0.001, divojaraca u odnosu na divokoze p<0.05, veprova u odnosu na krmače p<0.01 te krmača u odnosu na prasad p<0.05. U radu su također prikazane i morfološke osobitosti mikrocista parazita Sarcocystis spp. u poprečno-prugastom skeletnom i srčanom mišićju, prema histološkom prikazu. Mikrociste su bile ispunjene brojnim bradizoitima, smještenima unutar komorica