Molecules empowering animals to sense and respond to temperature in changing environments

Adapting behavior to thermal cues is essential for animal growth and survival. Indeed, each and every biological and biochemical process is profoundly affected by temperature and its extremes can cause irreversible damage. Hence, animals have developed thermotransduction mechanisms to detect and encode thermal information in the nervous system and acclimation mechanisms to finely tune their response over different timescales. While temperature-gated TRP channels are the best described class of temperature sensors, recent studies highlight many new candidates, including ionotropic and metabotropic receptors. Here, we review recent findings in vertebrate and invertebrate models, which highlight and substantiate the role of new candidate molecular thermometers and reveal intracellular signaling mechanisms implicated in thermal acclimation at the behavioral and cellular levels

Wavelength calibration of the JWST-MIRI medium resolution spectrometer

We present the wavelength and spectral resolution characterisation of the Integral Field Unit (IFU) Medium Resolution Spectrometer for the Mid-InfraRed Instrument (MIRI), to fly onboard the James Webb Space Telescope in 2014. We use data collected using the Verification Model of the instrument and develop an empirical method to calibrate properties such as wavelength range and resolving power in a portion of the spectrometer's full spectral range (5-28 microns). We test our results against optical models to verify the system requirements and combine them with a study of the fringing pattern in the instrument's detector to provide a more accurate calibration. We show that MIRI's IFU spectrometer will be able to produce spectra with a resolving power above R=2800 in the wavelength range 6.46-7.70 microns, and that the unresolved spectral lines are well fitted by a Gaussian profile.Comment: 12 pages, submitted to SPIE Proceedings vol. 7731, Space Telescopes and Instrumentation 2010: Optical, Infrared, and Millimeter Wav

Intragenic alternative splicing coordination is essential for Caenorhabditis elegans slo-1 gene function

Alternative splicing is critical for diversifying eukaryotic proteomes, but the rules governing and coordinating splicing events among multiple alternate splice sites within individual genes are not well understood. We developed a quantitative PCR-based strategy to quantify the expression of the 12 transcripts encoded by the Caenorhabditis elegans slo-1 gene, containing three alternate splice sites. Using conditional probability-based models, we show that splicing events are coordinated across these sites. Further, we identify a point mutation in an intron adjacent to one alternate splice site that disrupts alternative splicing at all three sites. This mutation leads to aberrant synaptic transmission at the neuromuscular junction. In a genomic survey, we found that a UAAAUC element disrupted by this mutation is enriched in introns flanking alternate exons in genes with multiple alternate splice sites. These results establish that proper coordination of intragenic alternative splicing is essential for normal physiology of slo-1 in vivo and identify putative specialized cis-regulatory elements that regulate the coordination of intragenic alternative splicing

Loss of CaMKI function disrupts salt aversive learning in C. elegans

The ability to adapt behavior to environmental fluctuations is critical for survival of organisms ranging from invertebrates to mammals. Caenorhabditis elegans can learn to avoid sodium chloride when it is paired with starvation. This behavior is likely advantageous to avoid areas without food. While some genes have been implicated in this salt aversive learning behavior, critical genetic components, and the neural circuit in which they act, remain elusive. Here, we show that the sole worm ortholog of mammalian CaMKI/IV, CMK-1, is essential for salt aversive learning behavior in C. elegans. We find that CMK-1 acts in the primary salt-sensing ASE neurons to regulate this behavior. By characterizing the intracellular calcium dynamics in ASE neurons using microfluidics, we find that loss of cmk-1 leads to an altered pattern of sensory- evoked calcium responses that may underlie salt aversive learning. Our study implicates the conserved CaMKI/CMK-1 as an essential cell-autonomous regulator for behavioral plasticity to environmental salt in C. elegans

Random-walk approach to mapping nodal regions of N-body wave functions: Ground-state Hartree-Fock wave functions for Li-C

Despite the widespread acceptance of the relevance of the nodes of one‐body electronic wave functions (atomic or molecular orbitals) in determining chemical properties, relatively little is known about the corresponding nodes of many‐body wave functions. As an alternative to mapping the nodal surfaces present in the ground states of many‐electron systems, we have focused instead on the structural domains implied by these surfaces. In the spirit of Monte Carlo techniques, the nodal hypervolumes of a series of atomic N‐body Hartree–Fock level electronic wave functions have been mapped using a random‐walk simulation in 3N dimensional configuration space. The basic structural elements of the domain of atomic or molecular wave functions are identified as nodal regions (continuous volumes of the same sign) and permutational cells (identical building blocks). Our algorithm determines both the relationships among nodal regions or cells (topology) as well as the geometric properties within each structural domain. Our results indicate that ground‐state Hartree–Fock wave functions generally consist of four equivalent nodal regions (two positive and two negative), each constructed from one or more permutational cells. We have developed an operational method to distinguish otherwise identical permutational cells. The limitations and most probable sources of error associated with this numerical method are discussed as are directions for future research

Thiamethoxam soil contaminations reduce fertility of soil-dwelling beetles, Aethina tumida.

There in increasing evidence for recent global insect declines. This is of major concern as insects play a critical role in ecosystem functionality and human food security. Even though environmental pollutants are known to reduce insect fertility, their potential effects on insect fitness remain poorly understood - especially for soil-dwelling species. Here, we show that fertility of soil-dwelling beetles, Aethina tumida, is reduced, on average, by half due to field-realistic neonicotinoid soil contaminations. In the laboratory, pupating beetles were exposed via soil to concentrations of the neonicotinoid thiamethoxam that reflect global pollution of agricultural and natural habitats. Emerged adult phenotypes and reproduction were measured, and even the lowest concentration reported from natural habitats reduced subsequent reproduction by 50%. The data are most likely a conservative estimate as the beetles were only exposed during pupation. Since the tested concentrations reflect ubiquitous soil pollution, the data reveal a plausible mechanism for ongoing insect declines. An immediate reduction in environmental pollutants is urgently required if our aim is to mitigate the prevailing loss of species biodiversity

Long-term prognosis of nerve palsy after total hip arthroplasty: results of two-year-follow-ups and long-term results after a mean time of 8years

Introduction: Nerve damage is a rare but serious complication after THA. There exist only little data about the outcome of these patients particularly regarding the long-term results later than 2years postoperatively. Aim of this study is to answer the following questions: Is the recovery to be expected for light nerve lesions different from the severe ones? Is there a possibility of nerve recovery more than 2years after THA? Is the potential of nerve recovery depending on the affected nerve? Materials and methods: This study investigates 2,255 primary THA as well as revision surgeries performed from 1988 to 2003 relating to iatrogenic nerve lesion. We classified the nerve lesion according to the core muscle strength in severe (M0-M2) and light (M3-M4) nerve damage and differentiated between femoral, sciatic and superior gluteal nerve, according to the electromyography. Results: We found 34 cases of iatrogenic nerve damage representing an incidence of 1.5%. 17 of 34 (50%) patients showed a complete recovery after 2years. Out of the remaining 17 patients, six out of seven patients with a final examination after a median time of 93months achieved further improvement. The different nerves showed no significant different potential of recovery. Conclusions: In contrast to the literature, an improvement beyond the limit of 2years is probable and independent of the nerve affected

Characterizing Exoplanets in the Visible and Infrared: A Spectrometer Concept for the EChO Space Mission

Transit-spectroscopy of exoplanets is one of the key observational techniques to characterize the extrasolar planet and its atmosphere. The observational challenges of these measurements require dedicated instrumentation and only the space environment allows an undisturbed access to earth-like atmospheric features such as water or carbon-dioxide. Therefore, several exoplanet-specific space missions are currently being studied. One of them is EChO, the Exoplanet Characterization Observatory, which is part of ESA's Cosmic Vision 2015-2025 program, and which is one of four candidates for the M3 launch slot in 2024. In this paper we present the results of our assessment study of the EChO spectrometer, the only science instrument onboard this spacecraft. The instrument is a multi-channel all-reflective dispersive spectrometer, covering the wavelength range from 400 nm to 16 microns simultaneously with a moderately low spectral resolution. We illustrate how the key technical challenge of the EChO mission - the high photometric stability - influences the choice of spectrometer concept and drives fundamentally the instrument design. First performance evaluations underline the fitness of the elaborated design solution for the needs of the EChO mission.Comment: 20 pages, 8 figures, accepted for publication in the Journal of Astronomical Instrumentatio

C2D Spitzer-IRS spectra of disks around T Tauri stars V. Spectral decomposition

(Abridged) Dust particles evolve in size and lattice structure in protoplanetary disks, due to coagulation, fragmentation and crystallization, and are radially and vertically mixed in disks. This paper aims at determining the mineralogical composition and size distribution of the dust grains in disks around 58 T Tauri stars observed with Spitzer/IRS. We present a spectral decomposition model that reproduces the IRS spectra over the full spectral range. The model assumes two dust populations: a warm component responsible for the 10\mu m emission arising from the disk inner regions and a colder component responsible for the 20-30\mu m emission, arising from more distant regions. We show evidence for a significant size distribution flattening compared to the typical MRN distribution, providing an explanation for the usual boxy 10\mu m feature profile generally observed. We reexamine the crystallinity paradox, observationally identified by Olofsson et al. (2009), and we find a simultaneous enrichment of the crystallinity in both the warm and cold regions, while grain sizes in both components are uncorrelated. Our modeling results do not show evidence for any correlations between the crystallinity and either the star spectral type, or the X-ray luminosity (for a subset of the sample). The size distribution flattening may suggests that grain coagulation is a slightly more effective process than fragmentation in disk atmospheres, and that this imbalance may last over most of the T Tauri phase. This result may also point toward small grain depletion via strong stellar winds or radiation pressure in the upper layers of disk. The non negligible cold crystallinity fractions suggests efficient radial mixing processes in order to distribute crystalline grains at large distances from the central object, along with possible nebular shocks in outer regions of disks that can thermally anneal amorphous grains

DEG/ENaC but Not TRP Channels Are the Major Mechanoelectrical Transduction Channels in a C. elegans Nociceptor

SummaryMany nociceptors detect mechanical cues, but the ion channels responsible for mechanotransduction in these sensory neurons remain obscure. Using in vivo recordings and genetic dissection, we identified the DEG/ENaC protein, DEG-1, as the major mechanotransduction channel in ASH, a polymodal nociceptor in Caenorhabditis elegans. But DEG-1 is not the only mechanotransduction channel in ASH: loss of deg-1 revealed a minor current whose properties differ from those expected of DEG/ENaC channels. This current was independent of two TRPV channels expressed in ASH. Although loss of these TRPV channels inhibits behavioral responses to noxious stimuli, we found that both mechanoreceptor currents and potentials were essentially wild-type in TRPV mutants. We propose that ASH nociceptors rely on two genetically distinct mechanotransduction channels and that TRPV channels contribute to encoding and transmitting information. Because mammalian and insect nociceptors also coexpress DEG/ENaCs and TRPVs, the cellular functions elaborated here for these ion channels may be conserved