Calibration procedure of regional flow duration curves evaluating water resource withdrawal from diversion dams

In the last decades, climatic changes in Mediterranean regions and frequent events of water resource scarcity in supply systems have required addressing the problem of increasing the inflows in storage reservoirs by connecting them to diversion dams. Usually, diversion dams do not have a large storage volume; consequently, these works are not able to regulate monthly flow, and they can divert to reservoirs only a part of river flow. In this field of research, this study aims to provide a procedure to evaluate water volumes withdrawn from diversion dams considering river flows and transfer flows. The procedure is founded by analysing 36 gauging stations in Sardinia (Italy), where a unique regional flow duration curve (FDC) can be defined. The effectiveness of regional evaluation of volume withdrawn, based on a monthly time scale analysis, has been investigated. The methodology allows finding optimal values of regional FDC parameters in order to better evaluate water resources withdrawn from diversion dams with respect to the current estimation used in the Sardinia Region Water Plan (SRWP). The current SRWP (RAS 2006) uses a fixed and extremely precautionary value of the FDC that underestimates withdrawal volumes. Moreover, a correlation analysis has been carried out in order to extend the evaluation of optimal FDC parameters for ungauged basins that allows to improve application of this procedure. Obtained results could allow to update the SRWP as highlighted in the final application to a real water system

Stochastic gradient methods for energy saving and a correct management in complex water supply systems

The management optimization of complex multi-source and multi-demand water resource systems under a high uncertainty level has been a subject of interest in the research literature (Labadie, 2004; Cunha & Sousa, 2010; Yuan et al., 2016). In this context, energy saving in operation of water pumping plants and reduction of water deficit for users and activities are frequently conflicting issues. Dealing with these problems, the definition of optimal activation rules for emergency activation of pumping stations are a relevant topic recently treated in Lerma et al. (2015) and Napolitano et al. (2016). In this study we want to define a trade-off between costs and risks considering the minimization of water shortage damages and the pumping operative costs, under different hydrological scenarios occurrences possibilities. Consequently, optimization results should provide the water system Authorities with a robust information about the optimal activation rules considering a large set of generated scenarios of hydrologic inputs to reservoirs. Using synthetic series it is possible to take into account the climate change impacts and balance the rules while also considering future behavior under the risk of the occurrence of shortages and the cost of early warning procedures to avoid water scarcity, mainly related to activation of emergency water transfers. Thereafter, this problem has been faced considering an efficient optimization tool based on the Stochastic Gradient method (SQG), see Ermoliev & Wets (1988) and Gaivoronski (2005). Testing the effectiveness of this proposal, an application of the modelling approach has been developed in a water shortage prone area in South-Sardinia (Italy)

A lymphofollicular microenvironment is required for pathological prion protein deposition in chronically inflamed tissues from scrapie-affected sheep

In sheep scrapie, pathological prion protein (PrPSc) deposition occurs in the lymphoreticular and central nervous systems. We investigated PrPSc distribution in scrapie- affected sheep showing simultaneous evidence of chronic lymphofollicular, lymphoproliferative/non-lymphofollicular,and/or granulomatous inflammations in their mammary gland, lung, and ileum. To do this, PrPSc detection was carried out via immunohistochemistry and Western Blotting techniques, as well as through inflammatory cell immunophenotyping. Expression studies of gene coding for biological factors modulating the host’s inflammatory response were also carried out. We demonstrated that ectopic PrPSc deposition occurs exclusively in the context of lymphofollicular inflammatory sites, inside newly formed and well-organized lymphoid follicles harboring follicular dendritic cells. On the contrary, no PrPSc deposition was detected in granulomas, even when they were closely located to newly formed lymphoid follicles. A significantly more consistent expression of lymphotoxin α and β mRNA was detected in lymphofollicular inflammation compared to the other two types, with lymphotoxin α and β signaling new lymphoid follicles’ formation and, likely, the occurrence of ectopic PrPSc deposition inside them. Our findings suggest that, in sheep co-affected by scrapie and chronic inflammatory conditions, only newly formed lymphoid follicles provide a suitable micro-environment that supports the scrapie agent’s replication in inflammatory sites, with an increased risk of prion shedding through body secretions/excretions.[...

Specific Immunoassays Confirm Association of Mycobacterium avium Subsp. paratuberculosis with Type-1 but Not Type-2 Diabetes Mellitus

Mycobacterium avium subspecies paratuberculosis (MAP) is a versatile pathogen with a broad host range. Its association with type-1 diabetes mellitus (T1DM) has been recently proposed. Rapid identification of infectious agents such as MAP in diabetic patients at the level of clinics might be helpful in deciphering the role of chronic bacterial infection in the development of autoimmune diseases such as T1DM.We describe use of an ELISA method to identify live circulating MAP through the detection of a cell envelope protein, MptD by a specific M13 phage--fMptD. We also used another ELISA format to detect immune response to MptD peptide. Both the methods were tested with blood plasma obtained from T1DM, type-2 diabetes (T2DM) patients and non-diabetic controls. Our results demonstrate MptD and fMptD ELISA assays to be accurate and sensitive to detect MAP bacilli in a large fraction (47.3%) of T1DM patients as compared to non-diabetic controls (12.6%) and those with confirmed T2DM (7.7%). Comparative analysis of ELISA assays performed here with 3 other MAP antigen preparations, namely HbHA, Gsd and whole cell MAP lysates confirmed comparable sensitivity of the MptD peptide and the fMptD based ELISA assays. Moreover, we were successful in demonstrating positive bacterial culture in two of the clinical specimen derived from T1DM patients.The MptD peptide/fMptD based ELISA or similar tests could be suggested as rapid and specific field level diagnostic tests for the identification of MAP in diabetic patients and for finding the explanations towards the occurrence of type-1 or type-2 diabetes in the light of an active infectious trigger

Assessment of evolutionary algorithms for optimal operating rules design in real Water Resource Systems

Two evolutionary algorithms (EAs) are assessed in this paper to design optimal operating rules (ORs) for Water Resource Systems (WRS). The assessment is established through a parameter analysis of both algorithms in a theoretical case, and the methodology described in this paper is applied to a complex, real case. These two applications allow us to analyse an algorithm's properties and performance by defining ORs, how an algorithm's termination/convergence criteria affect the results and the importance of decision-makers participating in the optimisation process. The former analysis reflects the need for correctly defining the important algorithm parameters to ensure an optimal result and how the greater number of termination conditions makes the algorithm an efficient tool for obtaining optimal ORs in less time. Finally, in the complex real case application, we discuss the participation value of decision-makers toward correctly defining the objectives and making decisions in the post-process. (C) 2014 Elsevier Ltd. All rights reserved.[EN]The authors wish to thank the University of Cagliari (Sardinia) and the Basin Agency of Sardinia for the data provided in the development of this study, as well as the Autonomous Region of Sardinia for funding the research project CRP 2_716. Thanks are also due to the Spanish Ministry of Science and Innovation (Comision Interministerial de Ciencia y Tecnologia, CICYT) for funding the projects NUTEGES (VI Plan Nacional de I+D-Ei 2008-2011, CGL2012-34978) and SCARCE (program Consolider-Ingenio 2010, project CSD2009-00065). The authors also thank the European Commission (Directorate-General for Research and Innovation) for funding the projects DROUGHT-R&SPI (program FP7-ENV-2011, project 282769), SIRIUS (FP7-SPACE-2010-1, project 262902), LIFE ALBUFERA project (LIFE12 ENV/ES/000685) and WAMCD project (EC-DG Environment No. 07.0329/2013/671291/SUB/ENV.C.1).Lerma Elvira, N.; Paredes Arquiola, J.; Andreu Álvarez, J.; Solera Solera, A.; Sechi, GM. (2015). Assessment of evolutionary algorithms for optimal operating rules design in real Water Resource Systems. Environmental Modelling and Software. 69:425-436. https://doi.org/10.1016/j.envsoft.2014.09.024S4254366

Haplotype Affinities Resolve a Major Component of Goat (Capra hircus) MtDNA D-Loop Diversity and Reveal Specific Features of the Sardinian Stock

Goat mtDNA haplogroup A is a poorly resolved lineage absorbing most of the overall diversity and is found in locations as distant as Eastern Asia and Southern Africa. Its phylogenetic dissection would cast light on an important portion of the spread of goat breeding. The aims of this work were 1) to provide an operational definition of meaningful mtDNA units within haplogroup A, 2) to investigate the mechanisms underlying the maintenance of diversity by considering the modes of selection operated by breeders and 3) to identify the peculiarities of Sardinian mtDNA types. We sequenced the mtDNA D-loop in a large sample of animals (1,591) which represents a non-trivial quota of the entire goat population of Sardinia. We found that Sardinia mirrors a large quota of mtDNA diversity of Western Eurasia in the number of variable sites, their mutational pattern and allele frequency. By using Bayesian analysis, a distance-based tree and a network analysis, we recognized demographically coherent groups of sequences identified by particular subsets of the variable positions. The results showed that this assignment system could be reproduced in other studies, capturing the greatest part of haplotype diversity

"Delirium Day": A nationwide point prevalence study of delirium in older hospitalized patients using an easy standardized diagnostic tool

Background: To date, delirium prevalence in adult acute hospital populations has been estimated generally from pooled findings of single-center studies and/or among specific patient populations. Furthermore, the number of participants in these studies has not exceeded a few hundred. To overcome these limitations, we have determined, in a multicenter study, the prevalence of delirium over a single day among a large population of patients admitted to acute and rehabilitation hospital wards in Italy. Methods: This is a point prevalence study (called "Delirium Day") including 1867 older patients (aged 65 years or more) across 108 acute and 12 rehabilitation wards in Italian hospitals. Delirium was assessed on the same day in all patients using the 4AT, a validated and briefly administered tool which does not require training. We also collected data regarding motoric subtypes of delirium, functional and nutritional status, dementia, comorbidity, medications, feeding tubes, peripheral venous and urinary catheters, and physical restraints. Results: The mean sample age was 82.0 \ub1 7.5 years (58 % female). Overall, 429 patients (22.9 %) had delirium. Hypoactive was the commonest subtype (132/344 patients, 38.5 %), followed by mixed, hyperactive, and nonmotoric delirium. The prevalence was highest in Neurology (28.5 %) and Geriatrics (24.7 %), lowest in Rehabilitation (14.0 %), and intermediate in Orthopedic (20.6 %) and Internal Medicine wards (21.4 %). In a multivariable logistic regression, age (odds ratio [OR] 1.03, 95 % confidence interval [CI] 1.01-1.05), Activities of Daily Living dependence (OR 1.19, 95 % CI 1.12-1.27), dementia (OR 3.25, 95 % CI 2.41-4.38), malnutrition (OR 2.01, 95 % CI 1.29-3.14), and use of antipsychotics (OR 2.03, 95 % CI 1.45-2.82), feeding tubes (OR 2.51, 95 % CI 1.11-5.66), peripheral venous catheters (OR 1.41, 95 % CI 1.06-1.87), urinary catheters (OR 1.73, 95 % CI 1.30-2.29), and physical restraints (OR 1.84, 95 % CI 1.40-2.40) were associated with delirium. Admission to Neurology wards was also associated with delirium (OR 2.00, 95 % CI 1.29-3.14), while admission to other settings was not. Conclusions: Delirium occurred in more than one out of five patients in acute and rehabilitation hospital wards. Prevalence was highest in Neurology and lowest in Rehabilitation divisions. The "Delirium Day" project might become a useful method to assess delirium across hospital settings and a benchmarking platform for future surveys