Assessment of the Effect of Satureja montana and Origanum virens Essential Oils on Aspergillus flavus Growth and Aflatoxin Production at Different Water Activities

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Aflatoxin contamination of foodstuffs poses a serious risk to food security, and it is essential to search for new control methods to prevent these toxins entering the food chain. Several essential oils are able to reduce the growth and mycotoxin biosynthesis of toxigenic species, although their efficiency is strongly influenced by the environmental conditions. In this work, the effectiveness of Satureja montana and Origanum virens essential oils to control Aspergillus flavus growth was evaluated under three water activity levels (0.94, 0.96 and 0.98 aw) using a Bioscreen C, a rapid in vitro spectrophotometric technique. The aflatoxin concentrations at all conditions tested were determined by HPLC-FLD. Aspergillus flavus growth was delayed by both essential oil treatments. However, only S. montana essential oil was able to significantly affect aflatoxin production, although the inhibition percentages widely differed among water activities. The most significant reduction was observed at 0.96 aw, which is coincident with the conditions in which A. flavus reached the highest levels of aflatoxin production. On the contrary, the treatment with S. montana essential oil was not effective in significantly reducing aflatoxin production at 0.94 aw. Therefore, it is important to study the interaction of the new control compounds with environmental factors before their application in food matrices, and in vitro ecophysiological studies are a good option since they provide accurate and rapid results.Peer reviewedFinal Published versio

Impact of night-time symptoms in COPD : a real-world study in five European countries

Peer reviewedPublisher PD

Factores que favorecen la inserción laboral de personas con discapacidad en Castellón

Setzenes Jornades de Foment de la Investigació (Any 2011)Es una realidad que las personas con discapacidad se encuentran en situación de desventaja a la hora de encontrar un puesto de trabajo. Teniendo en cuenta la actual situación del mercado laboral y las consecuencias que de ello se derivan, se decide observar las variables que influyen en el proceso de inserción laboral, centrándose en las personas con discapacidad, ya que por sus características presentan mayor vulnerabilidad. El objetivo principal es el conocimiento y fortalecimiento de las mismas para mejorar la inserción laboral de este colectivo. Las personas encuestadas han sido los responsables de recursos humanos de diferentes empresas de Castellón, a los que se les ha pasado una escala tipo Likert del 1 al 4 con el título: Cuestionario de valoración de las variables influyentes en la inserción laboral de personas con discapacidad, confeccionado por el grupo y validado por expertos. Las variables a estudiar fueron: Formación, Habilidades, Papel del trabajador social, Adaptaciones, Integración, Rendimiento laboral, Diferentes grupos de discapacidades, Imagen, Sector de Actividad y Características de la empresa: con o sin empleados con discapacidad. Finalmente la investigación sugiere que cada una de las variables propuestas son relevantes y favorecen, según los empresarios, a la hora de contratar al trabajador

Effect of aclidinium bromide on exacerbations in patients with moderate to severe COPD: a pooled analysis of five Phase III, randomized, placebo-controlled studies

We investigated the effect of the long-acting muscarinic antagonist aclidinium bromide on chronic obstructive pulmonary disease (COPD) exacerbations by pooling data from five randomized, placebo-controlled,parallel-group Phase III studies of 3-6 months' duration. Data were pooled from the aclidinium 400 μg twice-daily (BID) and placebo arms (N = 2,521) and stratified by Global initiative for chronic Obstructive Lung Disease (GOLD) group (A, B, C and D). Results showed that fewer patients experienced 1 exacerbation with aclidinium (any severity: 12.5%; moderate to severe: 10.9%) compared with placebo (any severity: 15.7%; moderate to severe: 13.3%) and the odds of experiencing 1 exacerbation of any severity were reduced in patients receiving aclidinium (odds ratio = 0.78, p = 0.039). Furthermore, aclidinium reduced the rate of exacerbations compared with placebo (any severity: rate ratio = 0.79, p = 0.026; moderate to severe: 0.80, p = 0.044). The time to first exacerbation of any severity was delayed with aclidinium compared with placebo (hazard ratio = 0.79, p = 0.026) and there was a numerical delay in time to first moderate-to-severe exacerbation. Finally, the effects of aclidinium on exacerbations versus placebo were greater in patients in GOLD Groups B and D; however, it is of note that only 10.7% of patients were classified in Group A or C. In summary, the results indicate that aclidinium 400 μg BID reduces the frequency of COPD exacerbations compared with placebo and that these effects are greater in symptomatic patients

ACTIVATE: the effect of aclidinium/formoterol on hyperinflation, exercise capacity, and physical activity in patients with COPD

The Phase IV, 8-week, randomized, double-blind, placebo-controlled ACTIVATE study (NCT02424344) evaluated the effect of aclidinium/formoterol (AB/FF) 400/12 mug twice daily on lung hyperinflation, exercise capacity, and physical activity in patients with moderate-to-severe COPD. Patients received AB/FF (n=134) or placebo (n=133) (1:1) via the Genuair/Pressair(R) dry powder inhaler for 8 weeks. From Weeks 5 to 8, all patients participated in behavioral intervention (BI; daily messages providing step goals). The primary end point was trough functional residual capacity (FRC) at Week 4. Exercise endurance time and physical activity were assessed at Week 4 (pharmacotherapy only) and at Week 8 (8 weeks of pharmacotherapy plus 4 weeks of BI). Other end points included post-dose FRC, residual volume, and inspiratory capacity (IC) at rest and during exercise. After 4 weeks, trough FRC improved with AB/FF versus placebo but did not reach significance (125 mL; P=0.0690). However, post-dose FRC, residual volume, and IC at rest improved significantly with AB/FF at Week 4 versus placebo (all P<0.0001). AB/FF significantly improved exercise endurance time and IC at isotime versus placebo at Week 4 (P<0.01 and P<0.0001, respectively) and Week 8 (P<0.05 and P<0.0001, respectively). AB/FF achieved higher step counts (P<0.01) with fewer inactive patients (P<0.0001) at Week 4 versus placebo. Following BI, AB/FF maintained improvements in physical activity at Week 8 and nonsignificant improvements were observed with placebo. AB/FF 400/12 mug demonstrated improvements in lung hyperinflation, exercise capacity, and physical activity versus placebo that were maintained following the addition of BI. A 4-week period of BI might be too short to augment the improvements of physical activity observed with AB/FF

Programa de col·laboració entre l’atenció a la salut mental i les addiccions, i l'atenció primària i comunitària

Salut mental; Addiccions; Atenció primàriaSalud mental; Adicciones; Atención primariaMental health; Addictions; Primary careAquest programa es basa, d’una banda, en el reforç i sistematització del treball conjunt entre els professionals de l’APiC i els de SMiA i, de l’altra, en el desplegament del model esglaonat d’atenció que consisteix en l’atribució de nivells assistencials diferenciats i procediments diagnòstics i terapèutics específics en funció de la gravetat clínica i la complexitat psicosocial de les persones ateses. Aquest model defineix què, qui i com s’atén en els diferents esglaons: promoció de la salut mental, prevenció de trastorns mentals en poblacions de risc, detecció precoç, diagnòstic àgil i tractament més eficient en cada cas

Altered machinery of protein synthesis is region- and stage-dependent and is associated with α-synuclein oligomers in Parkinson’s disease

Introduction: Parkinson's disease (PD) is characterized by the accumulation of abnormal a-synuclein in selected regions of the brain following a gradient of severity with disease progression. Whether this is accompanied by globally altered protein synthesis is poorly documented. The present study was carried out in PD stages 1-6 of Braak and middle-aged (MA) individuals without alterations in brain in the substantia nigra, frontal cortex area 8, angular gyrus, precuneus and putamen. Results: Reduced mRNA expression of nucleolar proteins nucleolin (NCL), nucleophosmin (NPM1), nucleoplasmin 3 (NPM3) and upstream binding transcription factor (UBF), decreased NPM1 but not NPM3 nucleolar protein immunostaining in remaining neurons; diminished 18S rRNA, 28S rRNA; reduced expression of several mRNAs encoding ribosomal protein (RP) subunits; and altered protein levels of initiation factor eIF3 and elongation factor eEF2 of protein synthesis was found in the substantia nigra in PD along with disease progression. Although many of these changes can be related to neuron loss in the substantia nigra, selective alteration of certain factors indicates variable degree of vulnerability of mRNAs, rRNAs and proteins in degenerating sustantia nigra. NPM1 mRNA and 18S rRNA was increased in the frontal cortex area 8 at stage 5-6; modifications were less marked and region-dependent in the angular gyrus and precuneus. Several RPs were abnormally regulated in the frontal cortex area 8 and precuneus, but only one RP in the angular gyrus, in PD. Altered levels of eIF3 and eIF1, and decrease eEF1A and eEF2 protein levels were observed in the frontal cortex in PD. No modifications were found in the putamen at any time of the study except transient modifications in 28S rRNA and only one RP mRNA at stages 5-6. These observations further indicate marked region-dependent and stage-dependent alterations in the cerebral cortex in PD. Altered solubility and a-synuclein oligomer formation, assessed in total homogenate fractions blotted with anti-a-synuclein oligomer-specific antibody, was demonstrated in the substantia nigra and frontal cortex, but not in the putamen, in PD. Dramatic increase in a-synuclein oligomers was also seen in fluorescent-activated cell sorter (FACS)-isolated nuclei in the frontal cortex in PD. Conclusions: Altered machinery of protein synthesis is altered in the substantia nigra and cerebral cortex in PD being the frontal cortex area 8 more affected than the angular gyrus and precuneus; in contrast, pathways of protein synthesis are apparently preserved in the putamen. This is associated with the presence of alpha-synuclein oligomeric species in total homogenates; substantia nigra and frontal cortex are enriched, albeit with different band patterns, in alpha-synuclein oligomeric species, whereas alpha-synuclein oligomers are not detected in the putamen

Connecting the microscopic depolarizing origin of samples with macroscopic measures of the Indices of Polarimetric Purity

In this work we show how a specific set of three depolarizing observables, the Indices of Polarimetric Purity (IPP), P1, P2 and P3, are ideal metrics to study the depolarization characteristic of media. We simulate different depolarizing scenarios, based on different depolarizing origins, and we study the corresponding IPP values. The simulations are based on the incoherent addition of multiple elemental polarizing elements, as ideal polarizers and/or retarders with different specific characteristics (orientation, retardance, transmittance, etc.). Further depolarizing scenarios are also studied by including the effect of ideal depolarizers. We show for the first time how by analyzing depolarizing systems through IPP we unravel two different depolarizing origins: isotropic and anisotropic depolarization, with meaningful physical interpretation. The former, isotropic depolarization is related to pure scattering processes, and mainly connected with P3 observable. The later, anisotropic depolarization is originated by microscopic constituent elements showing polarimetric anisotropy (dichroic and/or birefringent elements with different characteristics) and anisotropic scattering produced by these elements, and mainly described by P1 and P2 observables. Both effects can be simultaneously observed in real samples and give us information of the processes that give rise to depolarization in light-matter interactions. The simulated results are experimentally validated by analyzing the depolarizing behavior, in terms of IPP, of diverse real samples with easy physical interpretation, and direct connection with simulations. The present study could be of interest in multiple scenarios, to further understand the depolarizing response of samples, and it can be of special interest for the study of biological tissues and pathologies, as they present important depolarizing behavior.Monica Canabal-Carbia reports financial support was provided by Spain Ministry of Science and Innovation (PID2021-560 126509OB-C21 and PDC2022-133332-C21). Juan Campos reports financial support was provided by Spain Ministry of Science and Innovation (PID2021-560 126509OB-C21 and PDC2022-133332-C21). Angel Lizana reports financial support was provided by Spain Ministry of Science and Innovation (PID2021-560 126509OB-C21 and PDC2022-133332-C21). Irene Estevez reports financial support was provided by Government of Catalonia (Beatriu de Pinos, 2021-BP-00206). Ignacio Moreno reports financial support was provided by Spain Ministry of Science and Innovation (PID2021-126509OB-C22). Andres Marquez reports financial support was provided by Government of Valencia. Andres Marquez reports financial support was provided by Spain Ministry of Science and Innovation ( PID2021-123124OB-I00). Esther Nabadda reports financial support was provided by Government of Valencia. Mónica Canabal-Carbia, Angel Lizana and Juan Campos reports financial support was provided by the Generalitat de Catalunya (2021SGR00138)

Leptin levels were negatively associated with lumbar spine bone mineral content in children with overweight or obesity

Aim: Adipokines seem to play a role in bone morphogenesis, although this also depends on the mechanical forces applied to the skeleton. The aim was to assess the relationships of resting leptin and adiponectin with bone parameters and whether high muscular fitness levels affect these relationships in children with overweight or obesity. Methods: This cross-sectional study took part from 2014 to 2016 in Granada, Spain. Participants were recruited from University Hospitals, and we also used advertisements in local media and school contacts in the city. Adipokines were analysed in plasma. Muscular fitness was assessed by one repetition maximum in bench and leg press tests. Dual-energy X-ray absorptiometry was used to measure bone parameters. Results: We included 84 children (10.0 ± 1.2y; 63% boys) in this analysis. Leptin was negatively associated with lumbar spine bone mineral content (β = −0.162, p = 0.053)