The JCMT Gould Belt Survey: SCUBA-2 Data Reduction Methods and Gaussian Source Recovery Analysis

The James Clerk Maxwell Telescope (JCMT) Gould Belt Survey (GBS) was one of the first legacy surveys with the JCMT in Hawaii, mapping 47 deg2 of nearby (<500 pc) molecular clouds in dust continuum emission at 850 and 450 μm, as well as a more limited area in lines of various CO isotopologues. While molecular clouds and the material that forms stars have structures on many size scales, their larger-scale structures are difficult to observe reliably in the submillimeter regime using ground-based facilities. In this paper, we quantify the extent to which three subsequent data reduction methods employed by the JCMT GBS accurately recover emission structures of various size scales, in particular, dense cores, which are the focus of many GBS science goals. With our current best data reduction procedure, we expect to recover 100% of structures with Gaussian σ sizes of ≤30'' and intensity peaks of at least five times the local noise for isolated peaks of emission. The measured sizes and peak fluxes of these compact structures are reliable (within 15% of the input values), but source recovery and reliability both decrease significantly for larger emission structures and fainter peaks. Additional factors such as source crowding have not been tested in our analysis. The most recent JCMT GBS data release includes pointing corrections, and we demonstrate that these tend to decrease the sizes and increase the peak intensities of compact sources in our data set, mostly at a low level (several percent), but occasionally with notable improvement

TRAIL signals to apoptosis in chronic lymphocytic leukaemia cells primarily through TRAIL-R1 whereas cross-linked agonistic TRAIL-R2 antibodies facilitate signalling via TRAIL-R2

Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), a member of the TNF family, which is being developed as an anti-tumour agent due to its selective toxicity to tumour cells, induces apoptosis by binding to two membrane-bound receptors, TRAIL-R1 and TRAIL-R2. Clinical trials have been initiated with various preparations of TRAIL as well as agonistic monoclonal antibodies to TRAIL-R1 and TRAIL-R2. Previously we reported that prior treatment of primary chronic lymphocytic leukaemia (CLL) cells with histone deacetylase inhibitors was required to sensitize CLL cells to TRAIL and, using various receptor-selective TRAIL mutant ligands, we demonstrated that CLL cells signalled to apoptosis primarily through TRAIL-R1. Some, but not all, agonistic TRAIL-receptor antibodies require cross-linking in order to induce apoptosis. The present study demonstrated that CLL cells can signal to apoptosis through the TRAIL-R2 receptor, but only after cross-linking of the agonistic TRAIL-R2 antibodies, LBY135 and lexatumumab (HGS-ETR2). In contrast, signalling through TRAIL-R1 by receptor-selective ligands or certain agonistic antibodies, such as mapatumumab (HGS-ETR1), occurs in the absence of cross-linking. These results further highlight important differences in apoptotic signalling triggered through TRAIL-R1 and TRAIL-R2 in primary tumour cells. Such information is clearly important for the rational optimisation of TRAIL therapy in primary lymphoid malignancies, such as CLL

TRAIL signals to apoptosis in CLL cells primarily through TRAIL R-1 whereas cross-linked agonistic TRAIL R-2 antibodies facilitate signalling via TRAIL R-2

TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF family, which is being developed as an anti-tumour agent due to its selective toxicity to tumour cells, induces apoptosis by binding to two membrane-bound receptors, TRAIL-R1 and TRAIL-R2. Clinical trials have been initiated with various preparations of TRAIL as well as agonistic mAbs to TRAIL-R1 and TRAILR2. Previously we reported that prior treatment of primary chronic lymphocytic leukaemia (CLL) cells with histone deacetylase inhibitors (HDACi) was required to sensitize CLL cells to TRAIL and using various receptor-selective TRAIL mutant ligands we demonstrated that CLL cells signalled to apoptosis primarily through TRAIL-R1. Some, but not all, agonistic TRAIL-receptor antibodies require cross-linking in order to induce apoptosis. We now demonstrate that CLL cells can signal to apoptosis through the TRAIL-R2 receptor, but only after cross-linking of the agonistic TRAIL-R2 antibodies, LBY135 and lexatumumab (HGS-ETR2). In contrast, signalling through TRAIL-R1 by receptor-selective ligands or certain agonistic antibodies, such as mapatumumab (HGS-ETR1) occurs in the absence of cross-linking. These results further highlight important differences in apoptotic signalling triggered through TRAIL-R1 and TRAIL-R2 in primary tumour cells. Such information is clearly important for the rational optimisation of TRAIL therapy in primary lymphoid malignancies, such as CLL

The JCMT Gould Belt Survey: First results from SCUBA-2 observations of the Cepheus Flare region

We present observations of the Cepheus Flare obtained as part of the James Clerk Maxwell Telescope (JCMT) Gould Belt Legacy Survey (GBLS) with the SCUBA-2 instrument. We produce a catalogue of sources found by SCUBA-2, and separate these into starless cores and protostars. We determine masses and densities for each of our sources, using source temperatures determined by the Herschel Gould Belt Survey. We compare the properties of starless cores in four different molecular clouds: L1147/58, L1172/74, L1251 and L1228. We find that the core mass functions for each region typically show shallower-than-Salpeter behaviour. We find that L1147/58 and L1228 have a high ratio of starless cores to Class II protostars, while L1251 and L1174 have a low ratio, consistent with the latter regions being more active sites of current star formation, while the former are forming stars less actively. We determine that if modelled as thermally supported Bonnor–Ebert spheres, most of our cores have stable configurations accessible to them. We estimate the external pressures on our cores using archival 13CO velocity dispersion measurements and find that our cores are typically pressure confined, rather than gravitationally bound. We perform a virial analysis on our cores, and find that they typically cannot be supported against collapse by internal thermal energy alone, due primarily to the measured external pressures. This suggests that the dominant mode of internal support in starless cores in the Cepheus Flare is either non-thermal motions or internal magnetic fields.ISSN:0035-8711ISSN:1365-2966ISSN:1365-871

The JCMT Gould Belt Survey : a first look at SCUBA-2 observations of the Lupus I molecular cloud

This paper presents observations of the Lupus I molecular cloud at 450 and 850 μm with Submillimetre Common User Bolometer Array (SCUBA-2) as part of the James Clerk Maxwell Telescope Gould Belt Survey (JCMT GBS). Nine compact sources, assumed to be the discs of young stellar objects (YSOs), 12 extended protostellar, pre-stellar and starless cores, and one isolated, low-luminosity protostar, are detected in the region. Spectral energy distributions, including submillimetre fluxes, are produced for 15 YSOs, and each is fitted with the models of Robitaille et al. The proportion of Class 0/I protostars is higher than that seen in other Gould Belt regions such as Ophiuchus and Serpens. Circumstellar disc masses are calculated for more evolved sources, while protostellar envelope masses are calculated for protostars. Up to four very low luminosity objects are found; a large fraction when compared to other Spitzer c2d regions. One YSO has a disc mass greater than the minimum mass solar nebula. 12 starless/protostellar cores are detected by SCUBA-2 and their masses are calculated. The stability of these cores is examined using both the thermal Jeans mass and a turbulent virial mass when possible. Two cores in Lupus I are super-Jeans and contain no known YSOs. One of these cores has a virial parameter of 1.1 ± 0.4, and could therefore be pre-stellar. The high ratio of Class 0/I to Class III YSOs (1:1), and the presence of a pre-stellar core candidate, provides support for the hypothesis that a shock recently triggered star formation in Lupus I

The JCMT Gould Belt Survey : a first look at IC 5146

We present 450 and 850 μm submillimeter continuum observations of the IC 5146 star-forming region taken as part of the James Clerk Maxwell Telescope Gould Belt Survey. We investigate the location of bright submillimeter (clumped) emission with the larger-scale molecular cloud through comparison with extinction maps, and find that these denser structures correlate with higher cloud column density. Ninety-six individual submillimeter clumps are identified using FellWalker, and their physical properties are examined. These clumps are found to be relatively massive, ranging from 0.5 M⊙ to 116 M⊙ with a mean mass of 8 M⊙ and a median mass of 3.7 M⊙ . A stability analysis for the clumps suggests that the majority are (thermally) Jeans stable, with M/Mj < 1. We further compare the locations of known protostars with the observed submillimeter emission, finding that younger protostars, i.e., Class 0 and I sources, are strongly correlated with submillimeter peaks and that the clumps with protostars are among the most Jeans unstable. Finally, we contrast the evolutionary conditions in the two major star-forming regions within IC 5146: the young cluster associated with the Cocoon Nebula and the more distributed star formation associated with the Northern Streamer filaments. The Cocoon Nebula appears to have converted a higher fraction of its mass into dense clumps and protostars, the clumps are more likely to be Jeans unstable, and a larger fraction of these remaining clumps contain embedded protostars. The Northern Streamer, however, has a larger number of clumps in total and a larger fraction of the known protostars are still embedded within these clumps