Doubly pointed trisection diagrams and surgery on 2-knots

We study embedded spheres in 4-manifolds (2-knots) via doubly pointed trisection diagrams, showing that such descriptions are unique up to stabilization and handleslides, and we describe how to obtain trisection diagrams for certain cut-and-paste operations along 2-knots directly from doubly pointed trisection diagrams. The operations described are classical surgery, Gluck surgery, blowdown, and $(\pm4)$-rational blowdown, and we illustrate our techniques and results with many examples.Comment: 33 pages, 22 figure

Going Beyond Book Displays: Providing Safe Spaces for LGBTQ Youth

School libraries should be a space where students of all ages feel welcome and safe. I (Lisa) can speak from experience when I say this is not always the case, not in the 1980s and not today. Even in the school library, a place I now cherish, I was fearful of ridicule and harassment. I was frustrated because I did not see myself—a young, questioning, and confused lesbian—in any of the books. Gay and lesbian characters didn’t exist on those high school shelves. “But that was the 1980s,” you say. It hasn’t gotten much better for much of the LGBTQ community

Defect configurations and dynamical behavior in a Gay-Berne nematic emulsion

To model a nematic emulsion consisting of a surfactant-coated water droplet dispersed in a nematic host, we performed a molecular dynamics simulation of a droplet immersed in a system of 2048 Gay-Berne ellipsoids in a nematic phase. Strong radial anchoring at the surface of the droplet induced a Saturn ring defect configuration, consistent with theoretical predictions for very small droplets. A surface ring configuration was observed for lower radial anchoring strengths, and a pair of point defects was found near the poles of the droplet for tangential anchoring. We also simulated the falling ball experiment and measured the drag force anisotropy, in the presence of strong radial anchoring as well as zero anchoring strength.Comment: 17 pages, 15 figure

Comments on "Viscosity of high crystal content melts: dependence on solid fraction"

Here we propose a simple modification of the parameterisation presented in Costa (2005) [Viscosity of high crystal content melts: dependence on solid fraction, Geophys. Res. Lett., 32, 2005] which improves all the main positive features of it, yet at the same time, does not show a plateau region as the solid fraction tends to 1, generally increases the performance of the model, and exactly recovers the classical well established relationships valid for small fractions.Comment: 6 pages, 2 figure, comments on a previous paper published on Geophys. Res. Let

Vorinostat Renders the Replication-Competent Latent Reservoir of Human Immunodeficiency Virus (HIV) Vulnerable to Clearance by CD8 T Cells

Latently human immunodeficiency virus (HIV)-infected cells are transcriptionally quiescent and invisible to clearance by the immune system. To demonstrate that the latency reversing agent vorinostat (VOR) induces a window of vulnerability in the latent HIV reservoir, defined as the triggering of viral antigen production sufficient in quantity and duration to allow for recognition and clearance of persisting infection, we developed a latency clearance assay (LCA). The LCA is a quantitative viral outgrowth assay (QVOA) that includes the addition of immune effectors capable of clearing cells expressing viral antigen. Here we show a reduction in the recovery of replication-competent virus from VOR exposed resting CD4 T cells following addition of immune effectors for a discrete period. TAKE HOME MESSAGE: VOR exposure leads to sufficient production of viral protein on the cell surface, creating a window of vulnerability within this latent reservoir in antiretroviral therapy (ART)-suppressed HIV-infected individuals that allows the clearance of latently infected cells by an array of effector mechanisms

The litmus test of pride: analysing the emergence of the Belgrade “Ghost” pride in the context of EU accession

The transformation of lesbian, gay, bisexual, and transgender (LGBT) rights into a “standard for civilisation” has not been without consequences. With LGBT Pride parades becoming a symbol for Europeanness in the European Union (EU) accession process, this article asks how the litmus test character of Belgrade Pride has transformed LGBT politics in Serbia. Empirically, the analysis provides an in-depth analysis of how Serbia’s EU accession process has shaped the politics of Belgrade Pride between 2001 and 2015 and vice versa. It is argued that the international symbolic usage of Pride is no innocent practice as it has foreclosed its local politicality. Indeed, whilst Belgrade Pride became politicised as a litmus test in the EU accession process, domestically it developed into an apolitical ritualised event devoid of LGBT politics

IND-Enabling Studies for a Clinical Trial to Genetically Program a Persistent Cancer-Targeted Immune System

PURPOSE: To improve persistence of adoptively transferred T-cell receptor (TCR)-engineered T cells and durable clinical responses, we designed a clinical trial to transplant genetically-modified hematopoietic stem cells (HSCs) together with adoptive cell transfer of T cells both engineered to express an NY-ESO-1 TCR. Here, we report the preclinical studies performed to enable an investigational new drug (IND) application. EXPERIMENTAL DESIGN: HSCs transduced with a lentiviral vector expressing NY-ESO-1 TCR and the PET reporter/suicide gene HSV1-sr39TK and T cells transduced with a retroviral vector expressing NY-ESO-1 TCR were coadministered to myelodepleted HLA-A2/Kb mice within a formal Good Laboratory Practice (GLP)-compliant study to demonstrate safety, persistence, and HSC differentiation into all blood lineages. Non-GLP experiments included assessment of transgene immunogenicity and in vitro viral insertion safety studies. Furthermore, Good Manufacturing Practice (GMP)-compliant cell production qualification runs were performed to establish the manufacturing protocols for clinical use. RESULTS: TCR genetically modified and ex vivo-cultured HSCs differentiated into all blood subsets in vivo after HSC transplantation, and coadministration of TCR-transduced T cells did not result in increased toxicity. The expression of NY-ESO-1 TCR and sr39TK transgenes did not have a detrimental effect on gene-modified HSC's differentiation to all blood cell lineages. There was no evidence of genotoxicity induced by the lentiviral vector. GMP batches of clinical-grade transgenic cells produced during qualification runs had adequate stability and functionality. CONCLUSIONS: Coadministration of HSCs and T cells expressing an NY-ESO-1 TCR is safe in preclinical models. The results presented in this article led to the FDA approval of IND 17471

Linguistic Structures and Economic Outcomes

Linguistic structures have recently started to attract attention from economists as determinants of economic phenomena. This paper provides the first comprehensive review of this nascent literature and its achievements so far. First, we explore the complex connections between language, culture, thought and behaviour. Then, we summarize the empirical evidence on the relationship between linguistic structures and economic and social outcomes. We follow up with a discussion of data, empirical design and identification. The paper concludes by discussing implications for future research and policy