10 research outputs found
Increasing the light extraction and longevity of TMDC monolayers using liquid formed micro-lenses
The recent discovery of semiconducting two-dimensional materials is predicted to lead to the introduction of a series of revolutionary optoelectronic components that are just a few atoms thick. Key remaining challenges for producing practical devices from these materials lie in improving the coupling of light into and out of single atomic layers, and in making these layers robust to the influence of their surrounding environment. We present a solution to tackle both of these problems simultaneously, by deterministically placing an epoxy based micro-lens directly onto the materials’ surface. We show that this approach enhances the photoluminescence of tungsten diselenide (WSe2) monolayers by up to 300%, and nearly doubles the imaging resolution of the system. Furthermore, this solution fully encapsulates the monolayer, preventing it from physical damage and degradation in air. The optical solution we have developed could become a key enabling technology for the mass production of ultra-thin optical devices, such as quantum light emitting diodes
LPA1/3 receptor antagonist KI16425 as a novel treatment for the neurobehavioural effects of the ethanol
Aims. The lysophosphatidic acid (LPA) is an ubiquitous lysophospholipid that
acts through G-protein coupled receptors (LPA1-6), and it is involved in the
modulation of emotional and motivational behaviors. Recent literature suggests
a relevant role of the LPA signaling system in alcoholism, specially through the
LPA1 receptor. This work aims to elucidate whether systemic LPA1/3 receptor
blockade with ki16425 would modulate ethanol effects on the brain and behavior.
Methods. This study consisted of four experiments assessing the effect of
intraperitoneal ki16425 administration (20 mg/kg) on ethanol-related behaviors.
Male Wistar rats or mice (Swiss, C57BL/6J or hybrid C57BL/6JĂ—129X1/SvJ
background) were employed in various procedures: I) oral ethanol selfadministration;
II) loss of righting reflex; III) ethanol-induced conditioned place
preference (CPP) and IV) ethanol-withdrawal behavioral symptoms (by
assessing nest building, physical signs and spatial working memory).
Immunohistochemistry was carried out in order to evaluate basal neuronal activity
(c-Fos) in the medial prefrontal cortex (mPFC) and in the hippocampus, as well
as adult hippocampal neurogenesis (AHN) using proliferating cell nuclear antigen
(PCNA) and doublecortin (DCX) markers.
Results. Systemic Ki16425 administration reduced oral self-administration of
ethanol in previously trained rats. Likewise, ki16425 pretreatment in mice
attenuated the sedation induced by ethanol, blocked ethanol rewarding effect in
a CPP paradigm and reduced behavioral symptoms induced by ethanol
withdrawal. Immunohistochemistry revealed a protective effect of ki16425 against
ethanol actions on basal neuronal activity in the mPFC and on AHN.
Conclusions. Our results suggest a potential usefulness of systemic LPA1/3
receptors antagonists as a novel treatment for alcohol-related disorders.Universidad de Málaga, Campus de Excelencia
Internacional AndalucĂa Tech
Training memory without aversion: Appetitive hole-board spatial learning increases adult hippocampal neurogenesis.
Learning experiences are potent modulators of adult hippocampal neurogenesis (AHN). However, the vast majority of findings on the learning-induced regulation of AHN derive from aversively-motivated tasks, mainly the water maze paradigm, in which stress is a confounding factor that affects the AHN outcome. Currently, little is known regarding the effect of appetitively-motivated training on AHN. Hence we studied how spatial learning to find food rewards in a hole-board maze modulates AHN (cell proliferation and immature neurons) and AHN-related hippocampal neuroplasticity markers (BDNF, IGF-II and CREB phosphorylation) in mice. The 'Trained' mice were tested for both spatial reference and working memory and compared to 'Pseudotrained' mice (exposed to different baited holes in each session, thus avoiding the reference memory component of the task) and 'Control' mice (exposed to the maze without rewards). In contrast to Pseudotrained and Control mice, the number of proliferating hippocampal cells were reduced in Trained mice, but they notably increased their population of immature neurons assessed by immunohistochemistry. This evidence shows that hole-board spatial reference learning diminishes cell proliferation in favor of enhancing young neurons' survival. Interestingly, the enhanced AHN in the Trained mice (specifically in the suprapyramidal blade) positively correlated with their reference memory performance, but not with their working memory. Furthermore, the Trained animals increased the hippocampal protein expression of all the neuroplasticity markers analyzed by western blot. Results show that the appetitively-motivated hole-board task is a useful paradigm to potentiate and/or investigate AHN and hippocampal plasticity minimizing aversive variables such as fear or stress