Patient and implant-related factors associated to inflammatory profile in peri-implant crevicular fluid of implants placed in patients with history of aggressive and chronic periodontitis

Orientador: Márcio Zaffalon CasatiDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: O objetivo deste estudo de coorte foi determinar os fatores relacionados ao paciente e ao implante, associados a liberacao de citocinas no fluido crevicular peri-implantar (PICF), durante a cicatrizacao inicial e o processo de osseointegracao de implantes instalados em pacientes com historico de doenca periodontal agressiva e cronica. Para isso, por meio de envelopes pardos, foi aleatorizada a instalacao de noventa e dois implantes bone level ou tissue level, em pacientes parcialmente edentulos, apresentando historico de doenca periodontal agressiva ou cronica, ou saude periodontal. Aos 15 e 60 dias apos a instalacao dos implantes, foi realizada a coleta do PICF para avaliacao dos niveis de IL-1¿À, TNF-¿¿, IL-6, IL-8, IFN-¿Á, GM-CSF, IL-4, IL-10, IL-12 e IL-13, por meio da tecnologia Luminex/Magpix. Os fatores relacionados ao paciente e ao implante foram: genero, idade, condicao periodontal (saude, periodontite agressiva ou periodontite cronica), regiao de instalacao do implante (anterior ou posterior), torque de insercao (.15, 15-35, ou .35N), profundidade de sondagem peri-implantar, sangramento a sondagem, presenca ou ausencia de biofilme, analise da frequencia de ressonancia (ISQ), tipo de plataforma do implante (bone ou tissue level), comprimento do implante (8, 10 ou 12 mm), diametro do implante (3.3, 4.1 ou 4.8 mm), e diametro da plataforma do implante (3.3, 3.5, 4.1, 4.8, ou 6.5 mm). Os dados obtidos foram avaliados por regressao logistica multipla, considerando um nivel de significancia de 5%. A analise estatistica indicou que aos 15 dias, a liberacao de citocinas no PICF esta associada ao genero (GM-CSF), posicionamento do implante na arcada (IFN-¿Á, IL-4 e IL-8), torque de insercao (IFN-¿Á), sangramento a sondagem (IFN-¿Á), tipo de plataforma do implante (IFN-¿Á), historico de periodontite (IL-1¿À e IL-6), e presenca de biofilme (IL-8). Apos 60 dias da cirurgia de colocacao do implante, a liberacao de citocinas esteve associada a idade (GM-CSF, IL-6, IL-12 e IL-13), diametro da plataforma do implante (IFN-¿Á), profundidade de sondagem (IL-10), presenca de biofilme (IL-6 e IL-10), sangramento a sondagem (IL-12), posicionamento do implante na arcada (IL-1¿À), diametro do implante (IL-1¿À) e torque de insercao (IL-8). O comprimento do implante e a analise de frequencia de ressonancia nao estiveram associadas a liberacao das citocinas nos períodos avaliados. Deste modo, os resultados deste estudo sugerem que diferentes características relacionadas ao paciente e ao implante podem influenciar o conteúdo do fluído crevicular peri-implantar, durante a cicatrização inicial e o processo de osseointegração, em pacientes com histórico de doença periodontal agressiva e crônica.Abstract: The aim of this cohort study was to determine the implant and patient-related factors, associated to the release of peri-implant crevicular fluid (PICF), during early healing and osseointegration process of implants placed in patients with history of aggressive and chronic periodontal disease. For this, through brown envelopes, it was randomized the insertion of ninety-two bone level or tissue level implants, in patients with history of aggressive or chronic periodontitis, or periodontal health. At 15 and 60 days after implants insertion, PICF was collected to assessment the levels of IL-1¿À, TNF-¿¿, IL-6, IL-8, IFN-¿Á, GM-CSF, IL-4, IL-10, IL-12 and IL-13, using Luminex/Magpix assay. The implant and patient-related factor were: gender, age, periodontal condition (health, aggressive periodontitis, and chronic periodontitis), region of implant insertion (anterior or posterior), insertion torque (.15, 15-35, or .35N), peri-implant probing depth, bleeding on probing, presence or absence of biofilm, resonance frequency analysis (ISQ), type of implant platform (bone or tissue level), length of implant (8, 10 or 12 mm), diameter of implant (3.3, 4.1 or 4.8 mm), and diameter of implant platform (3.3, 3.5, 4.1, 4.8, or 6.5 mm). The data were assessed using multiple logistic regression analysis, with a significance level of 5%. The statistical analysis indicates that at 15 days cytokines release were influenced by gender (GM-CSF), position of implant in the arch (IFN-¿Á, IL-4, IL-8), insertion torque (IFN-¿Á), bleeding on probing (IFN-¿Á), type of implant platform (IFN-¿Á), periodontal condition (IL-1¿À, IL-6), and biofilm (IL-8). At 60 days cytokines release were influenced by age (GM-CSF, IL-6, IL-12, IL-13), diameter of implant platform (IFN-¿Á), probing depth (IL-10), biofilm (IL-6, IL-10), bleeding on probing (IL-12), position of implant in the arch (IL-1¿À), implant diameter (IL-1¿À) and insertion torque (IL-8). The length of implant and the resonance frequency analysis were factors not associated to the release of cytokines, in all follow-up periods. Thus, these results support that different implant and patient-related characteristics could influence the PICF composition during early healing and osseointegration process of dental implants placed in patients with a history of aggressive and chronic periodontitisMestradoPeriodontiaMestra em Clínica Odontológica2009/53921-0FAPESPCAPE

Improving Energy Conserving Descent for Machine Learning: Theory and Practice

We develop the theory of Energy Conserving Descent (ECD) and introduce ECDSep, a gradient-based optimization algorithm able to tackle convex and non-convex optimization problems. The method is based on the novel ECD framework of optimization as physical evolution of a suitable chaotic energy-conserving dynamical system, enabling analytic control of the distribution of results - dominated at low loss - even for generic high-dimensional problems with no symmetries. Compared to previous realizations of this idea, we exploit the theoretical control to improve both the dynamics and chaos-inducing elements, enhancing performance while simplifying the hyper-parameter tuning of the optimization algorithm targeted to different classes of problems. We empirically compare with popular optimization methods such as SGD, Adam and AdamW on a wide range of machine learning problems, finding competitive or improved performance compared to the best among them on each task. We identify limitations in our analysis pointing to possibilities for additional improvements.Comment: 15 pages + appendices, full code availabl

Measurement of sub-shot-noise spatial correlations without subtraction of background

In this paper we present the first measurement of sub-shot-noise spatial correlations without any subtraction of background, a result opening the way to realize sub-shot-noise imaging of weak objectsComment: 4 pages, 4 figure

The last 50 years of climate-induced melting of the Maliy Aktru glacier (Altai Mountains, Russia) revealed in a primary ecological succession

In this article, we report and discuss the results obtained from a survey of plants, microorganisms (bacteria and fungi), and soil elements along a chronosequence in the first 600 m of the Maliy Aktru glacier's forefront (Altai Mountains, Russia). Many glaciers of the world show effects of climate change. Nonetheless, except for some local reports, the ecological effects of deglaciation have been poorly studied and have not been quantitatively assessed in the Altai Mountains. Here, we studied the ecological changes of plants, fungi, bacteria, and soil elements that take the form of a primary ecological succession and that took place over the deglaciated soil of the Maliy Aktru glacier during the last 50 year. According to our measurements, the glacier lost about 12 m per year during the last 50 years. Plant succession shows clear signs of changes along the incremental distance from the glacier forefront. The analysis of the plant α‐ and β‐diversity confirmed an expected increase of them with increasing distance from the glacier forefront. Moreover, the analysis of β‐diversity confirmed the hypothesis of the presence of three main stages of the plant succession: (a) initial (pioneer species) from 30 to 100 m; (b) intermediate (r‐selected species) from 110 to 120–150 m; and (c) final (K‐selected species) from 150 to 550. Our study also shows that saprotrophic communities of fungi are widely distributed in the glacier retreating area with higher relative abundances of saprotroph ascomycetes at early successional stages. The evolution of a primary succession is also evident for bacteria, soil elements, and CO2 emission and respiration. The development of biological communities and the variation in geochemical parameters represent an irrefutable proof that climate change is altering soils that have been long covered by ice

The last 50 years of climate-induced melting of the Maliy Aktru glacier (Altai Mountains, Russia) revealed in a primary ecological succession

In this article, we report and discuss the results obtained from a survey of plants, microorganisms (bacteria and fungi), and soil elements along a chronosequence in the first 600 m of the Maliy Aktru glacier's forefront (Altai Mountains, Russia). Many glaciers of the world show effects of climate change. Nonetheless, except for some local reports, the ecological effects of deglaciation have been poorly studied and have not been quantitatively assessed in the Altai Mountains. Here, we studied the ecological changes of plants, fungi, bacteria, and soil elements that take the form of a primary ecological succession and that took place over the deglaciated soil of the Maliy Aktru glacier during the last 50 year. According to our measurements, the glacier lost about 12 m per year during the last 50 years. Plant succession shows clear signs of changes along the incremental distance from the glacier forefront. The analysis of the plant α‐ and β‐diversity confirmed an expected increase of them with increasing distance from the glacier forefront. Moreover, the analysis of β‐diversity confirmed the hypothesis of the presence of three main stages of the plant succession: (a) initial (pioneer species) from 30 to 100 m; (b) intermediate (r‐selected species) from 110 to 120–150 m; and (c) final (K‐selected species) from 150 to 550. Our study also shows that saprotrophic communities of fungi are widely distributed in the glacier retreating area with higher relative abundances of saprotroph ascomycetes at early successional stages. The evolution of a primary succession is also evident for bacteria, soil elements, and CO2 emission and respiration. The development of biological communities and the variation in geochemical parameters represent an irrefutable proof that climate change is altering soils that have been long covered by ice

Dermatoscopia no Granuloma Facial: Relato de 2 Casos

Granuloma faciale is a rare, benign, idiopathic skin disorder, observed predominantly in caucasian men between the third and fifth decades of life. It is characterized histologically by a mixed inflammatory infiltrate, in which phenomena of vasculitis and fibrosis are associated. It occurs predominantly in the face and has a chronic and slowly progressive course. Its diagnosis is based on clinical and histopathological features, with a recent emphasis on dermoscopy, which reveals aspects that may allow initial differentiation from other diagnoses (sarcoidosis, cutaneous lupus erythematosus, basal cell carcinoma, among others). There are several therapeutic options but with unsatisfactory results. In this article, we report two cases of patients with facial erythematous lesions in which dermoscopy was of great value as a diagnostic tool. Use of this technique will certainly grow in the coming years in relation to this pathology, since, besides providing important disease characteristics it represents a simple and easy way to identify facial granuloma.O granuloma facial é uma patologia cutânea rara, idiopática e benigna, com predomínio em homens caucasianos entre a terceira e quinta décadas de vida. Caracteriza-se histologicamente por um infiltrado inflamatório misto, em que fenômenos de vasculite e fibrose se associam, com predomínio na face, de curso crônico e lentamente progressivo. O diagnóstico baseia-se na história clinica, exame físico e histopatológico, com recente destaque para a dermatoscopia, que revela aspectos que podem possibilitar uma diferenciação inicial com outros diagnósticos (sarcoidose, lúpus eritematoso cutâneo, dentre outros). Existem várias opções terapêuticas propostas, porém com resultados pouco satisfatórios. Neste trabalho, relatamos dois casos de pacientes com lesões eritematosas faciais em que a dermatoscopia teve grande valor como ferramenta diagnóstica. O uso dessa modalidade deve crescer nos próximos anos em relação à essa patologia, já que, além de fornecer características importantes da doença, consiste em uma maneira simples e fácil de identificar o granuloma facial

A Happy Abundance : Tales, Memoirs and More Past and Present in Wayne, Maine

https://digitalmaine.com/wayne_books/1002/thumbnail.jp

Glomerular autoimmune multicomponents of human lupus nephritis in vivo: α-enolase and annexin AI

Renal targets of autoimmunity in human lupus nephritis (LN) are unknown. We sought to identify autoantibodies and glomerular target antigens in renal biopsy samples from patients with LN and determine whether the same autoantibodies can be detected in circulation. Glomeruli were microdissected from biopsy samples of 20 patients with LN and characterized by proteomic techniques. Serum samples from large cohorts of patients with systemic lupus erythematosus (SLE) with and without LN and other glomerulonephritides were tested. Glomerular IgGs recognized 11 podocyte antigens, with reactivity varying by LN pathology. Notably, IgG2 autoantibodies against α-enolase and annexin AI were detected in 11 and 10 of the biopsy samples, respectively, and predominated over other autoantibodies. Immunohistochemistry revealed colocalization of α-enolase or annexin AI with IgG2 in glomeruli. High levels of serum anti-α-enolase (>15 mg/L) IgG2 and/or anti-annexin AI (>2.7 mg/L) IgG2 were detected in most patients with LN but not patients with other glomerulonephritides, and they identified two cohorts: patients with high anti-α-enolase/low anti-annexin AI IgG2 and patients with low anti-α-enolase/high anti-annexin AI IgG2. Serum levels of both autoantibodies decreased significantly after 12 months of therapy for LN. Anti-α-enolase IgG2 recognized specific epitopes of α-enolase and did not cross-react with dsDNA. Furthermore, nephritogenic monoclonal IgG2 (clone H147) derived from lupus-prone MRL-lpr/lpr mice recognized human α-enolase, suggesting homology between animal models and human LN. These data show a multiantibody composition in LN, where IgG2 autoantibodies against α-enolase and annexin AI predominate in the glomerulus and can be detected in serum