Estudio histológico de huevos ováricos partenogenéticos de Moina eugeniae, Olivier 1954

Moina eugeniae is one of the most abundant zooplankton species found in ponds and lakes in Buenos Aires Province. Its importance is due to the common use of cladocerans as food fish. The aim of the present study was to investigate the reproductive structures through histological techniques. Samples were taken with a plankton net (150 μm) from the Calderón lake (Buenos Aires) during Spring 2006. The specimens were anesthetized with CO2 “in situ” and fixed with formaldehyde 4%. Parthenogenetic females in different reproductive stages were selected to perform with them routine histological techniques. No males were identified during our study. The ovaries are pair, secular and disposed dorsally in the body. The oocytes are polyedric with three different stages of maturation: previtellogenic (dense nuclear cromatine), vitellogenic (abundant yolk in cytoplasm and large granules of cromatine in the nucleous) and mature (abundant lipidic drops in cytoplasm). Many of the morphological features observed are similar to those of Daphnia genus. Further investigation is needed to elucidate the reproductive cycle of this species.Instituto de Limnología "Raúl A. Ringuelet

Estudio histológico de huevos ováricos partenogenéticos de Moina eugeniae, Olivier 1954

Moina eugeniae is one of the most abundant zooplankton species found in ponds and lakes in Buenos Aires Province. Its importance is due to the common use of cladocerans as food fish. The aim of the present study was to investigate the reproductive structures through histological techniques. Samples were taken with a plankton net (150 μm) from the Calderón lake (Buenos Aires) during Spring 2006. The specimens were anesthetized with CO2 “in situ” and fixed with formaldehyde 4%. Parthenogenetic females in different reproductive stages were selected to perform with them routine histological techniques. No males were identified during our study. The ovaries are pair, secular and disposed dorsally in the body. The oocytes are polyedric with three different stages of maturation: previtellogenic (dense nuclear cromatine), vitellogenic (abundant yolk in cytoplasm and large granules of cromatine in the nucleous) and mature (abundant lipidic drops in cytoplasm). Many of the morphological features observed are similar to those of Daphnia genus. Further investigation is needed to elucidate the reproductive cycle of this species.Moina eugeniae es una de las especies de más abundante del zooplancton las lagunas de la provincia de Buenos Aires. Su importancia se debe al uso común de los cladoceranos como alimento para peces. El objetivo del presente estudio fue investigar las estructuras reproductivas a través de técnicas histológicas. Las muestras se tomaron con una red de plancton (150 μm) del lago Calderón (Buenos Aires) durante la primavera de 2006. Las muestras se anestesiaron con CO2 in situ y se fijaron con formaldehído al 4%. Las hembras partenogenéticas en diferentes etapas reproductivas fueron seleccionadas para realizar con ellas técnicas histológicas de rutina. No se identificaron machos durante nuestro estudio. Los ovarios son pares, seculares y dispuestos dorsalmente en el cuerpo. Los ovocitos son poliédricos con tres etapas diferentes de maduración: previtelogénica (cromatina nuclear densa), vitelogénica (abundante yema en el citoplasma y grandes gránulos de cromatina en el núcleo) y maduras (abundantes gotas lipídicas en el citoplasma). Muchas de las características morfológicas observadas son similares a las del género Daphnia. Se necesita más investigación para dilucidar el ciclo reproductivo de esta especie

Estudio histológico de huevos ováricos partenogenéticos de Moina eugeniae, Olivier 1954

Moina eugeniae is one of the most abundant zooplankton species found in ponds and lakes in Buenos Aires Province. Its importance is due to the common use of cladocerans as food fish. The aim of the present study was to investigate the reproductive structures through histological techniques. Samples were taken with a plankton net (150 μm) from the Calderón lake (Buenos Aires) during Spring 2006. The specimens were anesthetized with CO2 “in situ” and fixed with formaldehyde 4%. Parthenogenetic females in different reproductive stages were selected to perform with them routine histological techniques. No males were identified during our study. The ovaries are pair, secular and disposed dorsally in the body. The oocytes are polyedric with three different stages of maturation: previtellogenic (dense nuclear cromatine), vitellogenic (abundant yolk in cytoplasm and large granules of cromatine in the nucleous) and mature (abundant lipidic drops in cytoplasm). Many of the morphological features observed are similar to those of Daphnia genus. Further investigation is needed to elucidate the reproductive cycle of this species.Instituto de Limnología "Raúl A. Ringuelet

Estudio histológico de huevos ováricos partenogenéticos de Moina eugeniae, Olivier 1954

Moina eugeniae is one of the most abundant zooplankton species found in ponds and lakes in Buenos Aires Province. Its importance is due to the common use of cladocerans as food fish. The aim of the present study was to investigate the reproductive structures through histological techniques. Samples were taken with a plankton net (150 μm) from the Calderón lake (Buenos Aires) during Spring 2006. The specimens were anesthetized with CO2 “in situ” and fixed with formaldehyde 4%. Parthenogenetic females in different reproductive stages were selected to perform with them routine histological techniques. No males were identified during our study. The ovaries are pair, secular and disposed dorsally in the body. The oocytes are polyedric with three different stages of maturation: previtellogenic (dense nuclear cromatine), vitellogenic (abundant yolk in cytoplasm and large granules of cromatine in the nucleous) and mature (abundant lipidic drops in cytoplasm). Many of the morphological features observed are similar to those of Daphnia genus. Further investigation is needed to elucidate the reproductive cycle of this species.Instituto de Limnología "Raúl A. Ringuelet

Double photo-ionization of He near a polarizable surface

We calculate the differential cross-section of the direct double photo-ionization of He physisorbed on a polarizable surface. By including the influence of the surface potential in the correlated two-electron final state wavefunction, we show that the differential cross-section carries detailed information on the electronic correlations at the surface. In particular, photo-emission along opposite directions, which is prohibited in the free space, is allowed if the surface potential is long-ranged.Comment: To appear in Phys. Rev. B - Rapid Comm. - 4 pages, 2 PostScript figures embedde

Olfactory Receptors in Non-Chemosensory Organs: The Nervous System in Health and Disease

Olfactory receptors (ORs) and down-stream functional signaling molecules adenylyl cyclase 3 (AC3), olfactory G protein \u3b1 subunit (G\u3b1olf), OR transporters receptor transporter proteins 1 and 2 (RTP1 and RTP2), receptor expression enhancing protein 1 (REEP1), and UDP-glucuronosyltransferases (UGTs) are expressed in neurons of the human and murine central nervous system (CNS). In vitro studies have shown that these receptors react to external stimuli and therefore are equipped to be functional. However, ORs are not directly related to the detection of odors. Several molecules delivered from the blood, cerebrospinal fluid, neighboring local neurons and glial cells, distant cells through the extracellular space, and the cells' own self-regulating internal homeostasis can be postulated as possible ligands. Moreover, a single neuron outside the olfactory epithelium expresses more than one receptor, and the mechanism of transcriptional regulation may be different in olfactory epithelia and brain neurons. OR gene expression is altered in several neurodegenerative diseases including Parkinson's disease (PD), Alzheimer's disease (AD), progressive supranuclear palsy (PSP) and sporadic Creutzfeldt-Jakob disease (sCJD) subtypes MM1 and VV2 with disease-, region- and subtype-specific patterns. Altered gene expression is also observed in the prefrontal cortex in schizophrenia with a major but not total influence of chlorpromazine treatment. Preliminary parallel observations have also shown the presence of taste receptors (TASRs), mainly of the bitter taste family, in the mammalian brain, whose function is not related to taste. TASRs in brain are also abnormally regulated in neurodegenerative diseases. These seminal observations point to the need for further studies on ORs and TASRs chemoreceptors in the mammalian brain