Discovery of a detached HI gas shell surrounding alpha Orionis

We report the detection of the HI line at 21 cm in the direction of alpha Ori with the Nancay Radiotelescope and with the Very Large Array. The observations confirm the previous detection of HI emission centered on alpha Ori, but additionally reveal for the first time a quasi-stationary detached shell of neutral atomic hydrogen ~4 arcmin. in diameter (0.24 pc at a distance of 200 pc). The detached shell appears elongated in a direction opposite to the star's space motion. A simple model shows that this detached atomic gas shell can result from the collision of the stellar wind from alpha Ori with the local interstellar medium (ISM). It implies that alpha Ori has been losing matter at a rate of ~ 1.2x10^-6 solar masses per year for the past 8x10^4 years. In addition, we report the detection of atomic hydrogen associated with the far-infrared arc located 6 arcmin. north-east of alpha Ori, that has been suggested to trace the bow shock resulting from the motion of the star through the ISM. We report also the detection by the Galaxy Evolution Explorer (GALEX) of a far-UV counterpart to this arc.Comment: Accepted for publication in the Monthly Notices; version with full resolution figures available at http://aramis.obspm.fr/~lebertre/paper-alphaOri_MNRAS.pd

Metal production in M33: space and time variations

Nearby galaxies are ideal places to study in detail metallicity gradients and their time evolution. We consider chemical abundances of a new sample of \hii\ regions complemented with previous literature data-sets. We compare \hii\ region and PN abundances obtained with a common set of observations taken at MMT. With an updated theoretical model, we follow the time evolution of the baryonic components and chemical abundances in the disk of M33, assuming that the galaxy is accreting gas from an external reservoir. Supported by a uniform sample of nebular spectroscopic observations, we conclude that: {\em i}) the metallicity distribution in M33 is very complex, showing a central depression in metallicity probably due to observational bias; {\em ii}) the metallicity gradient in the disk of M33 has a slope of -0.037$\pm$ 0.009 dex kpc$^{-1}$ in the whole radial range up to $\sim$8 kpc, and -0.044$\pm$ 0.009 dex kpc$^{-1}$ excluding the central kpc; {\em iii}) there is a small evolution of the slope with time from the epoch of PN progenitor formation to the present-time.}Comment: A&A accepted, 15 Pags, 13 Figs, language correctio

X Her and TX Psc: Two cases of ISM interaction with stellar winds observed by Herschel

The asymptotic giant branch (AGB) stars X Her and TX Psc have been imaged at 70 and 160 microns with the PACS instrument onboard the Herschel satellite, as part of the large MESS (Mass loss of Evolved StarS) Guaranteed Time Key Program. The images reveal an axisymmetric extended structure with its axis oriented along the space motion of the stars. This extended structure is very likely to be shaped by the interaction of the wind ejected by the AGB star with the surrounding interstellar medium (ISM). As predicted by numerical simulations, the detailed structure of the wind-ISM interface depends upon the relative velocity between star+wind and the ISM, which is large for these two stars (108 and 55 km/s for X Her and TX Psc, respectively). In both cases, there is a compact blob upstream whose origin is not fully elucidated, but that could be the signature of some instability in the wind-ISM shock. Deconvolved images of X Her and TX Psc reveal several discrete structures along the outermost filaments, which could be Kelvin-Helmholtz vortices. Finally, TX Psc is surrounded by an almost circular ring (the signature of the termination shock?) that contrasts with the outer, more structured filaments. A similar inner circular structure seems to be present in X Her as well, albeit less clearly.Comment: 11 pages, Astronomy & Astrophysics, in pres

Rescue of Dystrophic Skeletal Muscle by PGC-1α Involves a Fast to Slow Fiber Type Shift in the mdx Mouse

Increased utrophin expression is known to reduce pathology in dystrophin-deficient skeletal muscles. Transgenic over-expression of PGC-1α has been shown to increase levels of utrophin mRNA and improve the histology of mdx muscles. Other reports have shown that PGC-1α signaling can lead to increased oxidative capacity and a fast to slow fiber type shift. Given that it has been shown that slow fibers produce and maintain more utrophin than fast skeletal muscle fibers, we hypothesized that over-expression of PGC-1α in post-natal mdx mice would increase utrophin levels via a fiber type shift, resulting in more slow, oxidative fibers that are also more resistant to contraction-induced damage. To test this hypothesis, neonatal mdx mice were injected with recombinant adeno-associated virus (AAV) driving expression of PGC-1α. PGC-1α over-expression resulted in increased utrophin and type I myosin heavy chain expression as well as elevated mitochondrial protein expression. Muscles were shown to be more resistant to contraction-induced damage and more fatigue resistant. Sirt-1 was increased while p38 activation and NRF-1 were reduced in PGC-1α over-expressing muscle when compared to control. We also evaluated if the use a pharmacological PGC-1α pathway activator, resveratrol, could drive the same physiological changes. Resveratrol administration (100 mg/kg/day) resulted in improved fatigue resistance, but did not achieve significant increases in utrophin expression. These data suggest that the PGC-1α pathway is a potential target for therapeutic intervention in dystrophic skeletal muscle

Carbohydrate Metabolism Is Essential for the Colonization of Streptococcus thermophilus in the Digestive Tract of Gnotobiotic Rats

Streptococcus thermophilus is the archetype of lactose-adapted bacterium and so far, its sugar metabolism has been mainly investigated in vitro. The objective of this work was to study the impact of lactose and lactose permease on S. thermophilus physiology in the gastrointestinal tract (GIT) of gnotobiotic rats. We used rats mono-associated with LMD-9 strain and receiving 4.5% lactose. This model allowed the analysis of colonization curves of LMD-9, its metabolic profile, its production of lactate and its interaction with the colon epithelium. Lactose induced a rapid and high level of S. thermophilus in the GIT, where its activity led to 49 mM of intra-luminal L-lactate that was related to the induction of mono-carboxylic transporter mRNAs (SLC16A1 and SLC5A8) and p27Kip1 cell cycle arrest protein in epithelial cells. In the presence of a continuous lactose supply, S. thermophilus recruited proteins involved in glycolysis and induced the metabolism of alternative sugars as sucrose, galactose, and glycogen. Moreover, inactivation of the lactose transporter, LacS, delayed S. thermophilus colonization. Our results show i/that lactose constitutes a limiting factor for colonization of S. thermophilus, ii/that activation of enzymes involved in carbohydrate metabolism constitutes the metabolic signature of S. thermophilus in the GIT, iii/that the production of lactate settles the dialogue with colon epithelium. We propose a metabolic model of management of carbohydrate resources by S. thermophilus in the GIT. Our results are in accord with the rationale that nutritional allegation via consumption of yogurt alleviates the symptoms of lactose intolerance

Pseudomonas syringae pv. actinidiae (PSA) Isolates from Recent Bacterial Canker of Kiwifruit Outbreaks Belong to the Same Genetic Lineage

Intercontinental spread of emerging plant diseases is one of the most serious threats to world agriculture. One emerging disease is bacterial canker of kiwi fruit (Actinidia deliciosa and A. chinensis) caused by Pseudomonas syringae pv. actinidiae (PSA). The disease first occurred in China and Japan in the 1980s and in Korea and Italy in the 1990s. A more severe form of the disease broke out in Italy in 2008 and in additional countries in 2010 and 2011 threatening the viability of the global kiwi fruit industry. To start investigating the source and routes of international transmission of PSA, genomes of strains from China (the country of origin of the genus Actinidia), Japan, Korea, Italy and Portugal have been sequenced. Strains from China, Italy, and Portugal have been found to belong to the same clonal lineage with only 6 single nucleotide polymorphisms (SNPs) in 3,453,192 bp and one genomic island distinguishing the Chinese strains from the European strains. Not more than two SNPs distinguish each of the Italian and Portuguese strains from each other. The Japanese and Korean strains belong to a separate genetic lineage as previously reported. Analysis of additional European isolates and of New Zealand isolates exploiting genome-derived markers showed that these strains belong to the same lineage as the Italian and Chinese strains. Interestingly, the analyzed New Zealand strains are identical to European strains at the tested SNP loci but test positive for the genomic island present in the sequenced Chinese strains and negative for the genomic island present in the European strains. Results are interpreted in regard to the possible direction of movement of the pathogen between countries and suggest a possible Chinese origin of the European and New Zealand outbreaks

Pseudomonas viridiflava, a Multi Host Plant Pathogen with Significant Genetic Variation at the Molecular Level

The pectinolytic species Pseudomonas viridiflava has a wide host range among plants, causing foliar and stem necrotic lesions and basal stem and root rots. However, little is known about the molecular evolution of this species. In this study we investigated the intraspecies genetic variation of P. viridiflava amongst local (Cretan), as well as international isolates of the pathogen. The genetic and phenotypic variability were investigated by molecular fingerprinting (rep-PCR) and partial sequencing of three housekeeping genes (gyrB, rpoD and rpoB), and by biochemical and pathogenicity profiling. The biochemical tests and pathogenicity profiling did not reveal any variability among the isolates studied. However, the molecular fingerprinting patterns and housekeeping gene sequences clearly differentiated them. In a broader phylogenetic comparison of housekeeping gene sequences deposited in GenBank, significant genetic variability at the molecular level was found between isolates of P. viridiflava originated from different host species as well as among isolates from the same host. Our results provide a basis for more comprehensive understanding of the biology, sources and shifts in genetic diversity and evolution of P. viridiflava populations and should support the development of molecular identification tools and epidemiological studies in diseases caused by this species

Genome Sequence Analyses of Pseudomonas savastanoi pv. glycinea and Subtractive Hybridization-Based Comparative Genomics with Nine Pseudomonads

Bacterial blight, caused by Pseudomonas savastanoi pv. glycinea (Psg), is a common disease of soybean. In an effort to compare a current field isolate with one isolated in the early 1960s, the genomes of two Psg strains, race 4 and B076, were sequenced using 454 pyrosequencing. The genomes of both Psg strains share more than 4,900 highly conserved genes, indicating very low genetic diversity between Psg genomes. Though conserved, genome rearrangements and recombination events occur commonly within the two Psg genomes. When compared to each other, 437 and 163 specific genes were identified in B076 and race 4, respectively. Most specific genes are plasmid-borne, indicating that acquisition and maintenance of plasmids may represent a major mechanism to change the genetic composition of the genome and even acquire new virulence factors. Type three secretion gene clusters of Psg strains are near identical with that of P. savastanoi pv. phaseolicola (Pph) strain 1448A and they shared 20 common effector genes. Furthermore, the coronatine biosynthetic cluster is present on a large plasmid in strain B076, but not in race 4. In silico subtractive hybridization-based comparative genomic analyses with nine sequenced phytopathogenic pseudomonads identified dozens of specific islands (SIs), and revealed that the genomes of Psg strains are more similar to those belonging to the same genomospecies such as Pph 1448A than to other phytopathogenic pseudomonads. The number of highly conserved genes (core genome) among them decreased dramatically when more genomes were included in the subtraction, suggesting the diversification of pseudomonads, and further indicating the genome heterogeneity among pseudomonads. However, the number of specific genes did not change significantly, suggesting these genes are indeed specific in Psg genomes. These results reinforce the idea of a species complex of P. syringae and support the reclassification of P. syringae into different species