The gas turbulence in planetary nebulae: quantification and multi-D maps from long-slit, wide-spectral range echellogram

This methodological paper is part of a short series dedicated to the long-standing astronomical problem of de-projecting the bi-dimensional, apparent morphology of a three-dimensional distribution of gas. We focus on the quantification and spatial recovery of turbulent motions in planetary nebulae (and other classes of expanding nebulae) by means of long-slit echellograms over a wide spectral range. We introduce some basic theoretical notions, discuss the observational methodology, and develop an accurate procedure disentangling all broadening components of the velocity profile in all spatial positions of each spectral image. This allows us to extract random, non-thermal motions at unprecedented accuracy, and to map them in 1-, 2- and 3-dimensions. We present the solution to practical problems in the multi-dimensional turbulence-analysis of a testing-planetary nebula (NGC 7009), using the three-step procedure (spatio-kinematics, tomography, and 3-D rendering) developed at the Astronomical Observatory of Padua. In addition, we introduce an observational paradigm valid for all spectroscopic parameters in all classes of expanding nebulae. Unsteady, chaotic motions at a local scale constitute a fundamental (although elusive) kinematical parameter of each planetary nebula, providing deep insights on its different shaping agents and mechanisms, and on their mutual interaction. The detailed study of turbulence, its stratification within a target and (possible) systematic variation among different sub-classes of planetary nebulae deserve long-slit, multi-position angle, wide-spectral range echellograms containing emissions at low-, medium-, and high-ionization, to be analyzed pixel-to-pixel with a straightforward and versatile methodology, extracting all the physical information stored in each frame at best.Comment: 11 page, 10 figures, A&A in pres

A gene signature of 8 genes could identify the risk of recurrence and progression in Dukes' B colon cancer patients

The benefit of postoperative adjuvant chemotherapy in patients with Dukes' B colorectal cancer is still uncertain and its routine use is not recommended. The five-year relapse rate is approximately 25-40% and the identification of patients at high risk of recurrence would represent an important strategy for the use of adjuvant chemotherapy. We retrospectively analyzed gene expression profiles in frozen tumor specimens from patients with Dukes' B colorectal cancer by using high density oligonucleotide microarrays. Our results show a subset of 48 genes differentially expressed with an associated probability <0.001 in the t-test. Another statistical procedure based on the Fisher criterion resulted in 11 genes able to separate both groups. We selected the 8 genes present in both subsets. The differential expression of five genes (CHD2, RPS5, ZNF148, BRI3 and MGC23401) in colon cancer progression was confirmed by real-time PCR in an independent set of patients of Dukes' B and C stages

Patrones de desprendimiento de cítricos y respuesta del árbol bajo recolección mecanizada con sistemas sacudidores de copa

La recolección mecanizada es una importante alternativa para afrontar los problemas de disponibilidad de mano de obra, los costes de producción y mejorar la rentabilidad de la explotación. La viabilidad de la recolección mecanizada está marcada por la calidad de la fruta y la eficiencia su derribo y para ello es necesaria una adaptación entre máquina y árbol. El objetivo del trabajo es la determinación del patrón de abscisión de naranja dulce con sistema sacudidor de copa en comparación con otros sistemas de desprendimiento y, al mismo tiempo, establecer recomendaciones para alcanzar un elevado porcentaje de derribo. Se ensayaron 4 parcelas de naranja variedad ‘Valencia’ durante las campañas 2017/2018, empleando un sacudidor de copa Oxbo-3210. El patrón de desprendimiento según el tipo de recolección fue determinado junto el análisis de la vibración en la copa del árbol. La frecuencia de rotura tipo AZ-C predominó en caída natural (89,0%) y la recolección manual (79,5%) y se fue igualando con AZ-A en el sacudidor de copa (58,8%). La zona de contacto con las varas mostró un valor medio de ARMS de 2,3 veces mayor que la zona sin contacto, además de un 85% de desprendimiento frente a un 27%. Se definió que la forma de seto ancho debe ser adaptado para facilitar el acceso de las varas y que la máquina debe ajustarse entre el valor ARMS y el tiempo mayor de 300 ms-2 para lograr un desprendimiento del 100%

Olive Actual “on Year” Yield Forecast Tool Based on the Tree Canopy Geometry Using UAS Imagery

Olive has a notable importance in countries of Mediterranean basin and its profitability depends on several factors such as actual yield, production cost or product price. Actual “on year” Yield (AY) is production (kg tree-1) in “on years”, and this research attempts to relate it with geometrical parameters of the tree canopy. Regression equation to forecast AY based on manual canopy volume was determined based on data acquired from different orchard categories and cultivars during different harvesting seasons in southern Spain. Orthoimages were acquired with unmanned aerial systems (UAS) imagery calculating individual crown for relating to canopy volume and AY. Yield levels did not vary between orchard categories; however, it did between irrigated orchards (7000–17,000 kg ha-1) and rainfed ones (4000–7000 kg ha-1). After that, manual canopy volume was related with the individual crown area of trees that were calculated by orthoimages acquired with UAS imagery. Finally, AY was forecasted using both manual canopy volume and individual tree crown area as main factors for olive productivity. AY forecast only by using individual crown area made it possible to get a simple and cheap forecast tool for a wide range of olive orchards. Finally, the acquired information was introduced in a thematic map describing spatial AY variability obtained from orthoimage analysis that may be a powerful tool for farmers, insurance systems, market forecasts or to detect agronomical problems

Biomimetic Magnetic Nanocarriers Drive Choline Kinase Alpha Inhibitor inside Cancer Cells for Combined Chemo-Hyperthermia Therapy

Choline kinase a1 (ChoKa1) has become an excellent antitumor target. Among all the inhibitors synthetized, the new compound Ff35 shows an excellent capacity to inhibit ChoKa1 activity. However, soluble Ff35 is also capable of inhibiting choline uptake, making the inhibitor not selective for ChoKa1. In this study, we designed a new protocol with the aim of disentangling whether the Ff35 biological action is due to the inhibition of the enzyme and/or to the choline uptake. Moreover, we offer an alternative to avoid the inhibition of choline uptake caused by Ff35, since the coupling of Ff35 to novel biomimetic magnetic nanoparticles (BMNPs) allows it to enter the cell through endocytosis without interacting with the choline transporter. This opens the possibility of a clinical use of Ff35. Our results indicate that Ff35-BMNPs nanoassemblies increase the selectivity of Ff35 and have an antiproliferative effect. Also, we demonstrate the effectiveness of the tandem Ff35-BMNPs and hyperthermia.This research was funded by the Ministerio de Economía y Competitividad (CGL2013-46612 and CGL2016-76723 projects), Ramón y Cajal programme (RYC-2014-16901) and the Fondo Europeo de Desarrollo Regional (FEDER). Also, this research was aided by the Andalusian regional government (CTS-236)

Understanding kidney care needs and implementation strategies in low- and middle-income countries: conclusions from a "Kidney Disease: Improving Global Outcomes" (KDIGO) Controversies Conference

Evidence-based cinical practice guidelines improve delivery of uniform care to patients with and at risk of developing kidney disease, thereby reducing disease burden and improving outcomes. These guidelines are not well-integrated into care delivery systems in most low- and middle-income countries (LMICs). The KDIGO Controversies Conference on Implementation Strategies in LMIC reviewed the current state of knowledge in order to define a road map to improve the implementation of guideline-based kidney care in LMICs. An international group of multidisciplinary experts in nephrology, epidemiology, health economics, implementation science, health systems, policy, and research identified key issues related to guideline implementation. The issues examined included the current kidney disease burden in the context of health systems in LMIC, arguments for developing policies to implement guideline-based care, innovations to improve kidney care, and the process of guideline adaptation to suit local needs. This executive summary serves as a resource to guide future work, including a pathway for adapting existing guidelines in different geographical regions

Early efficacy evaluation of mesenchymal stromal cells (MSC) combined to biomaterials to treat long bone non-unions

Background and study aim: Advanced therapy medicinal products (ATMP) frequently lack of clinical data on efficacy to substantiate a future clinical use. This study aims to evaluate the efficacy to heal long bone delayed unions and non-unions, as secondary objective of the EudraCT 2011-005441-13 clinical trial, through clinical and radiological bone consolidation at 3, 6 and 12 months of follow-up, with subgroup analysis of affected bone, gender, tobacco use, and time since the original fracture. Patients and methods: Twenty-eight patients were recruited and surgically treated with autologous bone marrow derived mesenchymal stromal cells expanded under Good Manufacturing Practices, combined to bioceramics in the surgical room before implantation. Mean age was 39 ± 13 years, 57% were males, and mean Body Mass Index 27 ± 7. Thirteen (46%) were active smokers. There were 11 femoral, 4 humeral, and 13 tibial non-unions. Initial fracture occurred at a mean ± SD of 27.9 ± 31.2 months before recruitment. Efficacy results were expressed by clinical consolidation (no or mild pain if values under 30 in VAS scale), and by radiological consolidation with a REBORNE score over 11/16 points (value of or above 0.6875). Means were statistically compared and mixed models for repeated measurements estimated the mean and confidence intervals (95%) of the REBORNE Bone Healing scale. Clinical and radiological consolidation were analyzed in the subgroups with Spearman correlation tests (adjusted by Bonferroni). Results: Clinical consolidation was earlier confirmed, while radiological consolidation at 3 months was 25.0% (7/28 cases), at 6 months 67.8% (19/28 cases), and at 12 months, 92.8% (26/28 cases including the drop-out extrapolation of two failures). Bone biopsies confirmed bone formation surrounding the bioceramic granules. All locations showed similar consolidation, although this was delayed in tibial non-unions. No significant gender difference was found in 12-month consolidation (95% confidence). Higher consolidation scale values were seen in non-smoking patients at 6 (p = 0.012, t-test) and 12 months (p = 0.011, t-test). Longer time elapsed after the initial fracture did not preclude the occurrence of consolidation. Conclusion: Bone consolidation was efficaciously obtained with the studied expanded hBM-MSCs combined to biomaterials, by clinical and radiological evaluation, and confirmed by bone biopsies, with lower consolidation scores in smokers

Usual dietary treatment of gestational diabetes mellitus assessed after control diet in randomized controlled trials : subanalysis of a systematic review and meta-analysis

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