256 research outputs found
Radiation of Venus near lambda 1.35 cm
Theoretical and investigation calculations of Venus brightness temperature, and radio emission peculiarities due to presence of moisture in atmospher
Greenhouse effect in the atmosphere of Venus
Greenhouse effect in lower layers of Venus atmospher
Temperature dependence of the probability of "small heating" and total losses of ucns on the surface of fomblin oils of different molecular mass
We measured the temperature dependence of the probability of small heating
and total losses of UCNs on the PFPE Fomblin Y surface with various molecular
masses Mw=2800, 3300, 6500 amu in the temperature range of 100-300 K. The
probability of small heating sharply decreases with increasing Mw and
decreasing temperature. The probability of total loss weakly decreases with
decreasing temperature and takes the minimum value at Mw=3300 amu. As this oil
provides a homogeneous surface with minimal probabilities of small heating and
total losses of UCNs, it is the preferred candidate for experiments on
measuring the neutron lifetime
GRANIT project: a trap for gravitational quantum states of UCN
Previous studies of gravitationally bound states of ultracold neutrons showed
the quantization of energy levels, and confirmed quantum mechanical predictions
for the average size of the two lowest energy states wave functions.
Improvements in position-like measurements can increase the accuracy by an
order of magnitude only. We therefore develop another approach, consisting in
accurate measurements of the energy levels. The GRANIT experiment is devoted to
the study of resonant transitions between quantum states induced by an
oscillating perturbation.
According to Heisenberg's uncertainty relations, the accuracy of measurement
of the energy levels is limited by the time available to perform the
transitions. Thus, trapping quantum states will be necessary, and each source
of losses has to be controlled in order to maximize the lifetime of the states.
We discuss the general principles of transitions between quantum states, and
consider the main systematical losses of neutrons in a trap.Comment: presented in ISINN 15 seminar, Dubn
Conservation genetics of the pond bat (Myotis dasycneme) with special focus on the populations in northwestern Germany and in Jutland, Denmark
Conservation genetics is important in the management of endangered species, helping to understand their connectivity and longâterm viability, thus identifying populations of importance for conservation. The pond bat (Myotis dasycneme) is a rare species classified as âNear Threatenedâ with a wide but patchy Palearctic distribution. A total of 277 samples representing populations in Denmark, Germany, Latvia, Hungary, and Russia were used in the genetic analyses; 224 samples representing Denmark, Germany, and Russia were analyzed at 10 microsatellite loci; 241 samples representing all areas were analyzed using mitochondrial Dâloop and cytochrome B sequences. A Bayesian clustering approach revealed two poorly resolved clusters, one representing the Danish and German groups and the other the Russian group. However, significantly different pairwise FST and DEST estimates were observed between the Danish and German groups and between the Danish and Russian groups suggesting a recent population structure. These conflicting results might be attributed to the effect of migration or low resolution due to the number of microsatellite markers used. After concatenating the two mitochondrial sequences, analysis detected significant genetic differentiation between all populations, probably due to genetic drift combined with a founder event. The phylogenetic tree suggested a closer relationship between the Russian and Northern European populations compared to the Hungarian population, implying that the latter belongs to an older ancestral population. This was supported by the observed haplotype network and higher nucleotide diversity in this population. The genetic structuring observed in the Danish/German pond bat stresses the need for a crossâborder management between the two countries. Further, the pronounced mtDNA structuring, together with the indicated migration between nearby populations suggest philopatric female behavior but male migration, emphasizes the importance of protecting suitable habitat mosaics to maintain a continuum of patches with dense pond bat populations across the species' distribution range
SYNZIP Protein Interaction Toolbox: in Vitro and in Vivo Specifications of Heterospecific Coiled-Coil Interaction Domains
The synthetic biology toolkit contains a growing number of parts for regulating transcription and translation, but very few that can be used to control protein association. Here we report characterization of 22 previously published heterospecific synthetic coiled-coil peptides called SYNZIPs. We present biophysical analysis of the oligomerization states, helix orientations, and affinities of 27 SYNZIP pairs. SYNZIP pairs were also tested for interaction in two cell-based assays. In a yeast two-hybrid screen, >85% of 253 comparable interactions were consistent with prior in vitro measurements made using coiled-coil microarrays. In a yeast-signaling assay controlled by coiled-coil mediated scaffolding, 12 SYNZIP pairs were successfully used to down-regulate the expression of a reporter gene following treatment with α-factor. Characterization of these interaction modules dramatically increases the number of available protein interaction parts for synthetic biology and should facilitate a wide range of molecular engineering applications. Summary characteristics of 27 SYNZIP peptide pairs are reported in specification sheets available in the Supporting Information and at the SYNZIP Web site [http://keatingweb.mit.edu/SYNZIP/].National Science Foundation (U.S.) (NSF award MCB 0950233)National Institutes of Health (U.S.) (grant RO1 GM55040)National Institutes of Health (U.S.) (grant PN2 EY016546)National Institutes of Health (U.S.) (grant P50 GMO81879)National Science Foundation (U.S.). Synthetic Biology Engineering Research CenterHoward Hughes Medical Institut
CCBuilder:An interactive web-based tool for building, designing and assessing coiled-coil protein assemblies
Motivation: The ability to accurately model protein structures at the atomistic level underpins efforts to understand protein folding, to engineer natural proteins predictably and to design proteins de novo . Homology-based methods are well established and produce impressive results. However, these are limited to structures presented by and resolved for natural proteins. Addressing this problem more widely and deriving truly ab initio models requires mathematical descriptions for protein folds; the means to decorate these with natural, engineered or de novo sequences; and methods to score the resulting models.
Results: We present CCBuilder, a web-based application that tackles the problem for a defined but large class of protein structure, the α-helical coiled coils. CCBuilder generates coiled-coil backbones, builds side chains onto these frameworks and provides a range of metrics to measure the quality of the models. Its straightforward graphical user interface provides broad functionality that allows users to build and assess models, in which helix geometry, coiled-coil architecture and topology and protein sequence can be varied rapidly. We demonstrate the utility of CCBuilder by assembling models for 653 coiled-coil structures from the PDB, which cover >96% of the known coiled-coil types, and by generating models for rarer and de novo coiled-coil structures.
Availability and implementation: CCBuilder is freely available, without registration, at http://coiledcoils.chm.bris.ac.uk/app/cc_builder
Metabolic and morphological alterations induced by proteolysis-inducing factor from Walker tumour-bearing rats in C2C12 myotubes
BACKGROUND: Patients with advanced cancer suffer from cachexia, which is characterised by a marked weight loss, and is invariably associated with the presence of tumoral and humoral factors which are mainly responsible for the depletion of fat stores and muscular tissue. METHODS: In this work, we used cytotoxicity and enzymatic assays and morphological analysis to examine the effects of a proteolysis-inducing factor (PIF)-like molecule purified from ascitic fluid of Walker tumour-bearing rats (WF), which has been suggested to be responsible for muscle atrophy, on cultured C2C12 muscle cells. RESULTS: WF decreased the viability of C2C12 myotubes, especially at concentrations of 20-25 mug.mL-1. There was an increase in the content of the pro-oxidant malondialdehyde, and a decrease in antioxidant enzyme activity. Myotubes protein synthesis decreased and protein degradation increased together with an enhanced in the chymotrypsin-like enzyme activity, a measure of functional proteasome activity, after treatment with WF. Morphological alterations such as cell retraction and the presence of numerous cells in suspension were observed, particularly at high WF concentrations. CONCLUSION: These results indicate that WF has similar effects to those of proteolysis-inducing factor, but is less potent than the latter. Further studies are required to determine the precise role of WF in this experimental model. © 2008 Yano et al; licensee BioMed Central Ltd
A method to measure the resonance transitions between the gravitationally bound quantum states of neutrons in the GRANIT spectrometer
We present a method to measure the resonance transitions between the
gravitationally bound quantum states of neutrons in the GRANIT spectrometer.
The purpose of GRANIT is to improve the accuracy of measurement of the quantum
states parameters by several orders of magnitude, taking advantage of long
storage of Ultracold neutrons at specula trajectories. The transitions could be
excited using a periodic spatial variation of a magnetic field gradient. If the
frequency of such a perturbation (in the frame of a moving neutron) coincides
with a resonance frequency defined by the energy difference of two quantum
states, the transition probability will sharply increase. The GRANIT experiment
is motivated by searches for short-range interactions (in particular
spin-dependent interactions), by studying the interaction of a quantum system
with a gravitational field, by searches for extensions of the Standard model,
by the unique possibility to check the equivalence principle for an object in a
quantum state and by studying various quantum optics phenomena
Phylogenetic Relationships and Evolutionary Patterns of the Order Collodaria (Radiolaria)
Collodaria are the only group of Radiolaria that has a colonial lifestyle. This group is potentially the most important plankton in the oligotrophic ocean because of its large biomass and the high primary productivity associated with the numerous symbionts inside a cell or colony. The evolution of Collodaria could thus be related to the changes in paleo-productivity that have affected organic carbon fixation in the oligotrophic ocean. However, the fossil record of Collodaria is insufficient to trace their abundance through geological time, because most collodarians do not have silicified shells. Recently, molecular phylogeny based on nuclear small sub-unit ribosomal DNA (SSU rDNA) confirmed Collodaria to be one of five orders of Radiolaria, though the relationship among collodarians is still unresolved because of inadequate taxonomic sampling. Our phylogenetic analysis has revealed four novel collodarian sequences, on the basis of which collodarians can be divided into four clades that correspond to taxonomic grouping at the family level: Thalassicollidae, Collozoidae, Collosphaeridae, and Collophidae. Comparison of the results of our phylogenetic analyses with the morphological characteristics of each collodarian family suggests that the first ancestral collodarians had a solitary lifestyle and left no silica deposits. The timing of events estimated from molecular divergence calculations indicates that naked collodarian lineages first appeared around 45.6 million years (Ma) ago, coincident with the diversification of diatoms in the pelagic oceans. Colonial collodarians appeared after the formation of the present ocean circulation system and the development of oligotrophic conditions in the equatorial Pacific (ca. 33.4 Ma ago). The divergence of colonial collodarians probably caused a shift in the efficiency of primary production during this period
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