The HI content of star-forming galaxies at z = 0.24

We use observations from the Giant Metrewave Radio Telescope (GMRT) to measure the atomic hydrogen gas content of star-forming galaxies at z = 0.24 (i.e. a look-backtime of ~3 Gyr). The sample of galaxies studied were selected from Halpha-emitting field galaxies detected in a narrow-band imaging survey with the Subaru Telescope. The Anglo-Australian Telescope was used to obtain precise optical redshifts for these galaxies. We then coadded the HI 21 cm emission signal for all the galaxies within the GMRT spectral line data cube. From the coadded signal of 121 galaxies, we measure an average atomic hydrogen gas mass of (2.26 +- 0.90)*10^9 solar masses. We translate this HI signal into a cosmic density of neutral gas at z = 0.24 of Omega_gas = (0.91 +- 0.42)*10^-3. This is the current highest redshift at which Omega_gas has been constrained from 21 cm emission and our value is consistent with that estimated from damped Lyman-alpha systems around this redshift. We also find that the correlations between the Halpha luminosity and the radio continuum luminosity and between the star formation rate and the HI gas content in star-forming galaxies at z = 0.24 are consistent with the correlations found at z = 0. These two results suggest that the star formation mechanisms in field galaxies ~3 Gyr ago were not substantially different from the present, even though the star formation rate is 3 times higher.Comment: 11 pages, contains 9 figures and 1 table. Accepted for publishing in MNRAS 2007 January 22. Received 2007 January 22; in original form 2006 November 3

Immunochemical analysis of cathepsin B in lung tumours: an independent prognostic factor for squamous cell carcinoma patients

In order to evaluate the possible role of the proteolytic enzyme cathepsin B (cath B) in human non-small cell lung cancer (NSCLC) we examined cath B concentrations (cath Bc) and activities (cath BA) in homogenates of 127 pairs of lung tumour tissues and corresponding non-tumourous lung parenchyma. Total cath B activity (cath BAT) and enzymatic activity of the fraction of cath B, which is stable and active at pH 7.5 (cath BA7.5) were determined by a fluorogenic assay using synthetic substrate Z-Arg-Arg-AMC. The immunostaining pattern of cath B was determined in 239 lung tumour tissue sections, showing the presence of the enzyme in tumour cells (cath BT-I) and in tumour-associated histiocytes (cath BH-I). The median levels of cath BAT, cath BA7.5 and cath BC were 5.6-, 3.2- and 9.1-fold higher (P < 0.001), respectively, in tumour tissue than in non-tumourous lung parenchyma. Out of 131 tissue sections from patients with squamous cell carcinoma (SCC), 59.5% immunostained positively for cath B, while among the 108 adenocarcinoma (AC) patients 48.2% of tumours showed a positive reaction. There was a strong relationship between the levels of cath BAT, cath BA7.5, cath BC and cath BT-I in the primary tumours and the presence of lymph node metastases. Significant correlation with overall survival was observed for cath BT-I and cath BA7.5 (P < 0.01 and P < 0.05, respectively) in patients suffering from SCC. In these patients positive cath B in tumour cells (cath BT-I) and negative cath B in histiocytes (cath BH-I) indicated significantly shorter survival rate compared with patients with negative cath BT-I and positive cath BH-I (P < 0.0001). In contrast, in AC patients, both, positive cath BT-I and positive cath BH-I, indicated poor survival probability (P < 0.014). From these results we conclude that the proteolytic enzyme cath B is an independent prognostic factor for overall survival of patients suffering from SCC of the lung. © 1999 Cancer Research Campaig

Dominant Driving Forces in Human Telomere Quadruplex Binding-Induced Structural Alterations

Recently various pathways of human telomere (ht) DNA folding into G-quadruplexes and of ligand binding to these structures have been proposed. However, the key issue as to the nature of forces driving the folding and recognition processes remains unanswered. In this study, structural changes of 22-mer ht-DNA fragment (Tel22), induced by binding of ions (K(+), Na(+)) and specific bisquinolinium ligands, were monitored by calorimetric and spectroscopic methods and by gel electrophoresis. Using the global model analysis of a wide variety of experimental data, we were able to characterize the thermodynamic forces that govern the formation of stable Tel22 G-quadruplexes, folding intermediates, and ligand-quadruplex complexes, and then predict Tel22 behavior in aqueous solutions as a function of temperature, salt concentration, and ligand concentration. On the basis of the above, we believe that our work sets the framework for better understanding the heterogeneity of ht-DNA folding and binding pathways, and its structural polymorphism

A Radio and Optical Polarization Study of the Magnetic Field in the Small Magellanic Cloud

We present a study of the magnetic field of the Small Magellanic Cloud (SMC), carried out using radio Faraday rotation and optical starlight polarization data. Consistent negative rotation measures (RMs) across the SMC indicate that the line-of-sight magnetic field is directed uniformly away from us with a strength 0.19 +/- 0.06 microGauss. Applying the Chandrasekhar-Fermi method to starlight polarization data yields an ordered magnetic field in the plane of the sky of strength 1.6 +/- 0.4 microGauss oriented at a position angle 4 +/- 12 degs, measured counter-clockwise from the great circle on the sky joining the SMC to the Large Magellanic Cloud (LMC). We construct a three-dimensional magnetic field model of the SMC, under the assumption that the RMs and starlight polarization probe the same underlying large-scale field. The vector defining the overall orientation of the SMC magnetic field shows a potential alignment with the vector joining the center of the SMC to the center of the LMC, suggesting the possibility of a "pan-Magellanic'' magnetic field. A cosmic-ray driven dynamo is the most viable explanation of the observed field geometry, but has difficulties accounting for the observed uni-directional field lines. A study of Faraday rotation through the Magellanic Bridge is needed to further test the pan-Magellanic field hypothesis.Comment: 28 pages, 6 figures, accepted for publication in Ap

Cathepsins B, L and cystatin C in cyst fluid of ovarian tumors

Contains fulltext : 88032.pdf (publisher's version ) (Closed access)INTRODUCTION: In cancer, an extracellular and membrane bound localization of cathepsins contribute to the invasion of tumor cells at the basement membrane. METHODS: This is the first study that explored levels of cathepsins B (CatB), L (CatL) and their inhibitor cystatin C (CysC) in the cystic fluid (CF) of ovarian tumors (n = 110). RESULTS: CF contained considerable amounts of CatB, CatL and CysC. Remarkable differences in CatB and CatL and CysC CF levels were found between different histopathological tumor subtypes. Levels of CatB and CysC were significantly higher in CF of malignant serous tumors compared to those found in benign serous tumors (p = 0.010 and p = 0.001 respectively), whereas levels of CatL were significantly higher in CF of malignant mucinous tumors compared to those found in benign mucinous tumors (p = 0.035). CatB and CysC showed a strong correlation in the group of patients with malignant serous tumors (p < 0.001; R = 0.921) suggesting that the increase in CatB might be balanced by a corresponding increase in CysC. CONCLUSION: Further studies are warranted to investigate cathepsins as possible prognostic biomarkers for the aggressiveness of ovarian cancer.1 mei 201

Large-scale proteomic analysis of human brain identifies proteins associated with cognitive trajectory in advanced age

In advanced age, some individuals maintain a stable cognitive trajectory while others experience a rapid decline. Such variation in cognitive trajectory is only partially explained by traditional neurodegenerative pathologies. Hence, to identify new processes underlying variation in cognitive trajectory, we perform an unbiased proteome-wide association study of cognitive trajectory in a discovery (n = 104) and replication cohort (n = 39) of initially cognitively unimpaired, longitudinally assessed older-adult brain donors. We find 579 proteins associated with cognitive trajectory after meta-analysis. Notably, we present evidence for increased neuronal mitochondrial activities in cognitive stability regardless of the burden of traditional neuropathologies. Furthermore, we provide additional evidence for increased synaptic abundance and decreased inflammation and apoptosis in cognitive stability. Importantly, we nominate proteins associated with cognitive trajectory, particularly the 38 proteins that act independently of neuropathologies and are also hub proteins of protein co-expression networks, as promising targets for future mechanistic studies of cognitive trajectory.Accelerating Medicine Partnership for AD [U01AG046161, U01 AG061357]; Emory Alzheimer's Disease Research Center [P50 AG025688]; NINDS Emory Neuroscience Core [P30 NS055077]; intramural program of the National Institute on Aging (NIA); Alzheimer's Association; Alzheimer's Research UK; Michael J. Fox Foundation for Parkinson's Research; Weston Brain Institute Biomarkers Across Neurodegenerative Diseases Grant [11060]; National Institute of Neurological Disorders and Stroke [U24 NS072026]; National Institute on Aging [P30 AG19610]; Arizona Department of Health Services [211002]; Arizona Biomedical Research Commission [4001, 0011, 05-901, 1001]; [R01 AG056533]; [R01 AG053960]; [U01 MH115484]; [I01 BX003853]; [IK2 BX001820]; [R01 AG061800]; [R01 AG057911]Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

An Introduction to Gas Accretion onto Galaxies

Evidence for gas accretion onto galaxies can be found throughout the universe. In this chapter, I summarize the direct and indirect signatures of this process and discuss the primary sources. The evidence for gas accretion includes the star formation rates and metallicities of galaxies, the evolution of the cold gas content of the universe with time, numerous indirect indicators for individual galaxies, and a few direct detections of inflow. The primary sources of gas accretion are the intergalactic medium, satellite gas and feedback material. There is support for each of these sources from observations and simulations, but the methods with which the fuel ultimately settles in to form stars remain murky.Comment: 14 pages, 5 figures, Invited review to appear in Gas Accretion onto Galaxies, Astrophysics and Space Science Library, eds. A. J. Fox & R. Dav\'e, to be published by Springe

Detection of the Small Magellanic Cloud in gamma-rays with Fermi/LAT

The flux of gamma rays with energies >100MeV is dominated by diffuse emission from CRs illuminating the ISM of our Galaxy through the processes of Bremsstrahlung, pion production and decay, and inverse-Compton scattering. The study of this diffuse emission provides insight into the origin and transport of CRs. We searched for gamma-ray emission from the SMC in order to derive constraints on the CR population and transport in an external system with properties different from the Milky Way. We analysed the first 17 months of continuous all-sky observations by the Large Area Telescope of the Fermi mission to determine the spatial distribution, flux and spectrum of the gamma-ray emission from the SMC. We also used past radio synchrotron observations of the SMC to study the population of CR electrons specifically. We obtained the first detection of the SMC in high-energy gamma rays, with an integrated >100MeV flux of (3.7 +/-0.7) x10e-8 ph/cm2/s, with additional systematic uncertainty of <16%. The emission is steady and from an extended source ~3{\deg} in size. It is not clearly correlated with the distribution of massive stars or neutral gas, nor with known pulsars or SNRs, but a certain correlation with supergiant shells is observed. The observed flux implies an upper limit on the average CR nuclei density in the SMC of ~15% of the value measured locally in the Milky Way. The population of high-energy pulsars of the SMC may account for a substantial fraction of the gamma-ray flux, which would make the inferred CR nuclei density even lower. The average density of CR electrons derived from radio synchrotron observations is consistent with the same reduction factor but the uncertainties are large. From our current knowledge of the SMC, such a low CR density does not seem to be due to a lower rate of CR injection and rather indicates a smaller CR confinement volume characteristic size.Comment: 14 pages, 6 figures, accepted for publication in A&

The Enrichment of the Intergalactic Medium with Adiabatic Feedback I: Metal Cooling and Metal Diffusion

A study of the IGM metal enrichment using a series of SPH simulations is presented, employing metal cooling and turbulent diffusion of metals and thermal energy. An adiabatic feedback mechanism was adopted where gas cooling was prevented to generate galactic winds without explicit wind particles. The simulations produced a cosmic star formation history (SFH) that is broadly consistent with observations until z $\sim$ 0.5, and a steady evolution of the universal neutral hydrogen fraction ($\Omega_{\rm H I}$). At z=0, about 40% of the baryons are in the warm-hot intergalactic medium (WHIM), but most metals (80%-90%) are locked in stars. At higher z the proportion of metals in the IGM is higher due to more efficient loss from galaxies. The IGM metals primarily reside in the WHIM throughout cosmic history. The metallicity evolution of the gas inside galaxies is broadly consistent with observations, but the diffuse IGM is under enriched at z $\sim$ 2.5. Galactic winds most efficiently enrich the IGM for halos in the intermediate mass range $10^{10}$M_{\sun} - $10^{11}$ M_{\sun}. At the low mass end gas is prevented from accreting onto halos and has very low metallicities. At the high mass end, the fraction of halo baryons escaped as winds declines along with the decline of stellar mass fraction of the galaxies. This is likely because of the decrease in star formation activity and in wind escape efficiency. Metals enhance cooling which allows WHIM gas to cool onto galaxies and increases star formation. Metal diffusion allows winds to mix prior to escape, decreasing the IGM metal content in favour of gas within galactic halos and star forming gas. Diffusion significantly increases the amount of gas with low metallicities and changes the density-metallicity relation.Comment: 18 pages, 11 figures, Accepted for publication in MNRA

Grey and white matter correlates of recent and remote autobiographical memory retrieval:Insights from the dementias

The capacity to remember self-referential past events relies on the integrity of a distributed neural network. Controversy exists, however, regarding the involvement of specific brain structures for the retrieval of recently experienced versus more distant events. Here, we explored how characteristic patterns of atrophy in neurodegenerative disorders differentially disrupt remote versus recent autobiographical memory. Eleven behavioural-variant frontotemporal dementia, 10 semantic dementia, 15 Alzheimer's disease patients and 14 healthy older Controls completed the Autobiographical Interview. All patient groups displayed significant remote memory impairments relative to Controls. Similarly, recent period retrieval was significantly compromised in behavioural-variant frontotemporal dementia and Alzheimer's disease, yet semantic dementia patients scored in line with Controls. Voxel-based morphometry and diffusion tensor imaging analyses, for all participants combined, were conducted to investigate grey and white matter correlates of remote and recent autobiographical memory retrieval. Neural correlates common to both recent and remote time periods were identified, including the hippocampus, medial prefrontal, and frontopolar cortices, and the forceps minor and left hippocampal portion of the cingulum bundle. Regions exclusively implicated in each time period were also identified. The integrity of the anterior temporal cortices was related to the retrieval of remote memories, whereas the posterior cingulate cortex emerged as a structure significantly associated with recent autobiographical memory retrieval. This study represents the first investigation of the grey and white matter correlates of remote and recent autobiographical memory retrieval in neurodegenerative disorders. Our findings demonstrate the importance of core brain structures, including the medial prefrontal cortex and hippocampus, irrespective of time period, and point towards the contribution of discrete regions in mediating successful retrieval of distant versus recently experienced events