Avaliação Numérica Da Estabilidade Elástica De Cantoneiras Conectadas Pelas Abas Por Meio DE MEF E GBT / Numerical Assessment Of The Elastic Stability Of Cornerstones Connected By The Tabs Through MEF AND GBT

Os procedimentos de dimensionamento de estruturas metálicas tradicionais envolvem um procedimento indireto, para o qual os esforços solicitantes sobre cada barra (obtidos por meio da análise estrutural) são comparados com os esforços resistentes, os quais são obtidos de forma isolada, assumindo-se para a barra condições de apoio idealizadas (e.g.: apoios simples, engastes, etc.). Assim, procura-se neste trabalho avaliar a influência de tais hipóteses sobre os resultados de estabilidade elástica desses perfis, analisando-se as mesmas barras sob condições simplificadas, e também sob condições mais realistas, compatíveis com as disposições construtivas a que são submetidos os elementos em estudo. No caso específico de cantoneiras de aço de abas iguais, ilustram-se essas diferenças com a ajuda de análises de estabilidade elástica realizadas com o Método dos Elementos Finitos (MEF) e com a Teoria Generalizada de Vigas (GBT), evidenciando um estudo pormenorizado dos fatores que envolvem as análises e dimensionamento desses elementos. Nas cantoneiras analisadas sob compressão excêntrica observa-se que muitos elementos estruturais acabam sendo dimensionados baseando-se em condições de apoio idealizadas, enquanto na realidade tais condições não se verificam

Análisis de las comorbilidades en pacientes con colitis ulcerosa: una herramienta para prevenir las exacerbaciones en casos de colitis ulcerosa

There have been previous studies, especially in Western countries and even in some areas in Asia, about extra-intestinal manifestations (EIMs) and its link with the outcome of inflammatory bowel disease (IBD), which includes Crohn’s disease (CD), and ulcerative colitis (UC). This link is crucial when discussing a patient’s prognosis and important when dealing with UC management. The aim of this study was to clarify the most common comorbidities associated with UC, emphasizing immunologic comorbidities in Japan. This study was a retrospective analysis performed at Nagoya University Hospital. The data collection started in March, 2019, and continued for two years. We retrieved the medical records of 105 patients with UC diagnosis, from which the data of 176 EIMs were extracted and analyzed. Results showed that EIMs with UC in the active phase accounted for 43.7% of total EIMs. Twenty-six patients with immune-mediated inflammatory disease frequently had an active phase (odds ratio [OR] 3.84, 99% CI, 1.44–10.27). Comorbidities showing an active manifestation of symptoms and UC in the active phase were significantly correlated in patients with immunological comorbidities, such as peripheral arthritis (r = 0.97, p < 0.01) and rheumatoid arthritis (RA) (r = 0.99, p < 0.01), as well as in patients with primary sclerosis cholangitis (PSC) (r = 0.98, p < 0.01). In conclusion, this analysis suggests the importance of having full comprehension of how immunological comorbidities affect the natural development of UC, which is of vital importance to prevent further UC complications and properly adjust the management of the disease.Se trata de un análisis retrospectivo que estudia múltiples comorbilidades de índole inmunológico que se da en pacientes con colitis ulcerosa. Este estudio se llevó a cabo en el hospital universitario de la Universidad de Nagoya. Se recolectó datos de 105 pacientes con colitis ulcerosa, de los cuales 176 comorbilidades extraintestinales fueron analizadas. Se encontró que comorbilidades con manifestación activa de síntomas y con colitis ulcerosa en fase activa fueron significativamente correlacionadas en pacientes con comorbilidades inmunológicas, tales como artritis periféricas (r=O.97, P<O.OI ), artritis reumatoide (r=O.99, P<O.OI ), así como pacientes con colangitis esclerosa primaria (r=O.98, P<O.OI ). En conclusión, este análisis sugiere la importancia de tener plena comprensión de cómo las comorbilidades inmunológicas afectan el desarrollo natural de la colitis ulcerosa, lo cual es de vital importancia para prevenir mayores complicaciones de la colitis ulcerosa y ajustar adecuadamente el manejo de la enfermedad.Japón. Ministerio de Educación, Cultura, Deportes, Ciencia y Tecnología (Monbukagakusho)Artículo de investigació

Early-stage antibody kinetics after the third dose of BNT162b2 mRNA COVID-19 vaccination measured by a point-of-care fingertip whole blood testing

Amid the Coronavirus Disease 2019 pandemic, we aimed to demonstrate the accuracy of the fingertip whole blood sampling test (FWT) in measuring the antibody titer and uncovering its dynamics shortly after booster vaccination. Mokobio SARS-CoV-2 IgM & IgG Quantum Dot immunoassay (Mokobio Biotechnology R&D Center Inc., MD, USA) was used as a point-of-care FWT in 226 health care workers (HCWs) who had received two doses of the BNT162b2 mRNA vaccine (Pfizer-BioNTech) at least 8 months prior. Each participant tested their antibody titers before and after the third-dose booster up to 14-days. The effect of the booster was observed as early as the fourth day after vaccination, which exceeded the detection limit (>30,000 U/mL) by 2.3% on the fifth day, 12.2% on the sixth day, and 22.5% after the seventh day. Significant positive correlations were observed between the pre- and post-vaccination (the seventh and eighth days) antibody titers (correlation coefficient, 0.405; p<0.001). FWT is useful for examining antibody titers as a point-of-care test. Rapid response of antibody titer started as early as the fourth day post-vaccination, while the presence of weak responders to BNT162b2 vaccine was indicated

ショチュウトウ キョウイク ニホンゴ キョウシ ケンシュウ ニ オケル キョウジュホウ ジュギョウ ニ ツイテ

初・中等教育段階の日本語教育は、各国・地域の外国語教育政策に基づいて展開されており、シラバス、カリキュラム、教材もそれぞれの国・地域で統一されている場合が多く、学習者の発達段階など多くの要因を考慮しながら授業を組み立てていく必要がある。初・中等教育の教師を対象とした研修の教授法授業では、このような背景を考慮した実践的な教授活動を取り入れていくことが重要である。2003年度海外日本語教師短期研修(春期)(以下、春短期研修)における教授法授業では以下の試みを行った。できる限り教授環境を共有する国・地域別のクラス編成を重視し、多国籍のクラスの人数を少数になるようにした。さらに、それぞれの教授環境を重視し、その一方で国・地域を超えた初・中等教育に共通する課題をその枠組みの中で取り上げることとした。 共通に扱う項目としては、それぞれの教師が普段行っているであろう教授活動の流れ、「教授目標設定→授業設計→授業準備・教材作成→授業実施→授業に対する評価」の流れを教授法授業で経験させること、コンピュータの利用、高校訪問という要素を、それぞれの教師の望む程度に応じて教授活動の中に織り込ませることであった。 本稿では、5クラスそれぞれの教授法授業の実際を報告し、コンピュータ利用、高校訪問については全体について報告した。研修に参加したほとんどの教師は、教授法授業のクラス分け、扱った内容について役立ったとしているが、違う国・地域の教師との情報交流の機会をより増やすことを望む声が多かった。さらに、コンピュータ利用を勧めたことについては、IT普及の如何に関わらず好評であった。高校訪問については、半数程度が役立つとしているが、問題があったとする意見もあり、教授法授業への取り入れ方については検討を要する。今後も、初・中等教育に携わる日本語教師への研修を改善していくためには、実践に根ざした情報の蓄積が必要である

Expression and purification of recombinant G protein-coupled receptors: A review

Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this, many of these receptors have limited or no available prophylaxis. Novel drug design and discovery significantly rely on structure determination, of which GPCRs are typically elusive. Progress has been made thus far to produce sufficient quantity and quality of protein for downstream analysis. As such, this review highlights the systems available for recombinant GPCR expression, with consideration of their advantages and disadvantages, as well as examples of receptors successfully expressed in these systems. Additionally, an overview is given on the use of detergents and the styrene maleic acid (SMA) co-polymer for membrane solubilisation, as well as purification techniques

Endothelial Dysfunction, Increased Arterial Stiffness, and Cardiovascular Risk Prediction in Patients With Coronary Artery Disease: FMD‐J (Flow‐Mediated Dilation Japan) Study A

BackgroundThe usefulness of vascular function tests for management of patients with a history of coronary artery disease is not fully known.Methods and ResultsWe measured flow‐mediated vasodilation (FMD) and brachial–ankle pulse wave velocity (baPWV) in 462 patients with coronary artery disease for assessment of the predictive value of FMD and baPWV for future cardiovascular events in a prospective multicenter observational study. The first primary outcome was coronary events, and the second primary outcome was a composite of coronary events, stroke, heart failure, and sudden death. During a median follow‐up period of 49.2 months, the first primary outcome occurred in 56 patients and the second primary outcome occurred in 66 patients. FMD above the cutoff value of 7.1%, derived from receiver‐operator curve analyses for the first and second primary outcomes, was significantly associated with lower risk of the first (hazard ratio, 0.27; 95% confidence interval, 0.06–0.74; P=0.008) and second (hazard ratio, 0.32; 95% confidence interval, 0.09–0.79; P=0.01) primary outcomes. baPWV above the cutoff value of 1731 cm/s was significantly associated with higher risk of the first (hazard ratio, 1.86; 95% confidence interval, 1.01–3.44; P=0.04) and second (hazard ratio, 2.19; 95% confidence interval, 1.23–3.90; P=0.008) primary outcomes. Among 4 groups stratified according to the combination of cutoff values of FMD and baPWV, stepwise increases in the calculated risk ratio for the first and second primary outcomes were observed.ConclusionsIn patients with coronary artery disease, both FMD and baPWV were significant predictors of cardiovascular events. The combination of FMD and baPWV provided further cardiovascular risk stratification