28 research outputs found
Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes: Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating
One possible factor determining recovery of trace amount of protein biomarker
candidates during proteome analyses could be adsorption on urine tubes. This
issue, however, has not been well addressed so far. Recently, a new technical
device of surface coating by poly(2-methacryloyloxyethyl phosphorylcholine
(MPC)-co-n-butyl methacrylate (BMA))
(poly(MPC-co-BMA)) has been developed mainly to prevent the
adsorption of plasma proteins. We assessed whether conventionally used urine
tubes adsorb trace amount of urinary proteins and, if any, whether the surface
coating by poly(MPC-co-BMA) can minimize the adsorption.
Proteinuric urine samples were kept in poly(MPC-co-BMA)-coated
and noncoated urine tubes for 15 min and possibly adsorbed proteins and/or
peptides onto urine tubes were analyzed by SDS-PAGE, 2-DE, and the MALDI-TOF MS.
It was found that a number of proteins and/or peptides adsorb on the
conventionally used urine tubes and that surface coating by
poly(MPC-co-BMA) can minimize the adsorption without any
significant effects on routine urinalysis test results. Although it remains to
be clarified to what extent the protein adsorption can modify the results of
urinary proteome analyses, one has to consider this possible adsorption of
urinary proteins when searching for trace amounts of protein biomarkers in
urine
NBRP, National Bioresource Project of Japan and plant bioresource management
The National BioResource Project has been organized and established to promote research activities using valuable bioresources. A total of twenty-eight bioresources for ten animals, nine plants and nine microorganisms/cell lines developed or collected in Japan were selected for the project. Resources are categorized into several different groups in the project; genetic resources, germplasm, genome resources and their information. Choices of how many resources must be preserved and maintained and in which categories are dependent on the status of the research community of each organism. These resources, if utilized systematically and intelligently, are powerful means for leading new scientific discoveries. Some examples can be seen in this paper. This paper reviews plant bioresources with the main focus on rice resource activities within the project
Source-Frequency Phase-Referencing Observation of AGNs with KaVA Using Simultaneous Dual-Frequency Receiving
The KVN(Korean VLBI Network)-style simultaneous multi-frequency receiving
mode is demonstrated to be promising for mm-VLBI observations. Recently, other
Very long baseline interferometry (VLBI) facilities all over the globe start to
implement compatible optics systems. Simultaneous dual/multi-frequency VLBI
observations at mm wavelengths with international baselines are thus possible.
In this paper, we present the results from the first successful simultaneous
22/43 GHz dual-frequency observation with KaVA(KVN and VERA array), including
images and astrometric results. Our analysis shows that the newly implemented
simultaneous receiving system has brought a significant extension of the
coherence time of the 43 GHz visibility phases along the international
baselines. The astrometric results obtained with KaVA are consistent with those
obtained with the independent analysis of the KVN data. Our results thus
confirm the good performance of the simultaneous receiving systems for the
non-KVN stations. Future simultaneous observations with more global stations
bring even higher sensitivity and micro-arcsecond level astrometric
measurements of the targets.Comment: 8 pages, 6 figures, Published in JKA
Pilot KaVA monitoring on the M87 jet: confirming the inner jet structure and superluminal motions at sub-pc scales
We report the initial results of our high-cadence monitoring program on the
radio jet in the active galaxy M87, obtained by the KVN and VERA Array (KaVA)
at 22 GHz. This is a pilot study that preceded a larger KaVA-M87 monitoring
program, which is currently ongoing. The pilot monitoring was mostly performed
every two to three weeks from December 2013 to June 2014, at a recording rate
of 1 Gbps, obtaining the data for a total of 10 epochs. We successfully
obtained a sequence of good quality radio maps that revealed the rich structure
of this jet from <~1 mas to 20 mas, corresponding to physical scales
(projected) of ~0.1-2 pc (or ~140-2800 Schwarzschild radii). We detected
superluminal motions at these scales, together with a trend of gradual
acceleration. The first evidence for such fast motions and acceleration near
the jet base were obtained from recent VLBA studies at 43 GHz, and the fact
that very similar kinematics are seen at a different frequency and time with a
different instrument suggests these properties are fundamental characteristics
of this jet. This pilot program demonstrates that KaVA is a powerful VLBI array
for studying the detailed structural evolution of the M87 jet and also other
relativistic jets.Comment: 10 pages, 9 figures, accepted for publication in PAS
Precessing jet nozzle connecting to a spinning black hole in M87
The nearby radio galaxy M87 offers a unique opportunity to explore the
connections between the central supermassive black hole and relativistic jets.
Previous studies of the inner region of M87 revealed a wide opening angle for
the jet originating near the black hole. The Event Horizon Telescope resolved
the central radio source and found an asymmetric ring structure consistent with
expectations from General Relativity. With a baseline of 17 years of
observations, there was a shift in the jet's transverse position, possibly
arising from an eight to ten-year quasi-periodicity. However, the origin of
this sideways shift remains unclear. Here we report an analysis of radio
observations over 22 years that suggests a period of about 11 years in the
position angle variation of the jet. We infer that we are seeing a spinning
black hole that induces the Lense-Thirring precession of a misaligned accretion
disk. Similar jet precession may commonly occur in other active galactic nuclei
but has been challenging to detect owing to the small magnitude and long period
of the variation.Comment: 41 pages, 7 figures, 7 table
Broadband Multi-wavelength Properties of M87 during the 2017 Event Horizon Telescope Campaign
Abstract: In 2017, the Event Horizon Telescope (EHT) Collaboration succeeded in capturing the first direct image of the center of the M87 galaxy. The asymmetric ring morphology and size are consistent with theoretical expectations for a weakly accreting supermassive black hole of mass ∼6.5 × 109 M ⊙. The EHTC also partnered with several international facilities in space and on the ground, to arrange an extensive, quasi-simultaneous multi-wavelength campaign. This Letter presents the results and analysis of this campaign, as well as the multi-wavelength data as a legacy data repository. We captured M87 in a historically low state, and the core flux dominates over HST-1 at high energies, making it possible to combine core flux constraints with the more spatially precise very long baseline interferometry data. We present the most complete simultaneous multi-wavelength spectrum of the active nucleus to date, and discuss the complexity and caveats of combining data from different spatial scales into one broadband spectrum. We apply two heuristic, isotropic leptonic single-zone models to provide insight into the basic source properties, but conclude that a structured jet is necessary to explain M87’s spectrum. We can exclude that the simultaneous γ-ray emission is produced via inverse Compton emission in the same region producing the EHT mm-band emission, and further conclude that the γ-rays can only be produced in the inner jets (inward of HST-1) if there are strongly particle-dominated regions. Direct synchrotron emission from accelerated protons and secondaries cannot yet be excluded