Didáctica del español en los estudios de traducción

Decenes Jornades de Foment de la Investigació de la FCHS (Any 2004-2005

Rediseño Pikis Labis. Un caso real

Pikis Labis, como se podrá leer a continuación, es una cafetería-bistro bar sevillano, ubicada en la calle Alfonso XII, en pleno centro de la ciudad, que tiene un encanto muy peculiar. Necesitaba urgentemente un cambio, una buena promoción para darse a conocer y que el público pudiera disfrutar. A lo largo del documento se podrá ver la evolución, contrastando cómo estaban antes a cómo están ahora, además de ver planes y ambiciones futuras que quiere el negocio. Primero comenzaremos con la documentación de la marca — apartado compuesto por el briefing y contrabriefing —; luego pasaremos a la investigación, tanto interna como externa, de Pikis Labis, entendiendo sus raíces y situación en la que se encuentra para que, por último, se vea el bloque más relevante de este trabajo: el rediseño de la marca y la implementación de las acciones llevadas a cabo.Universidad de Sevilla. Grado en Publicidad y Relaciones Pública

El aprendizaje basado en problemas: revisión de estudios empíricos internacionales

p. 397-418En este artículo, se analizan, mediante la revisión de artículos científicos sobre el tema, los resultados obtenidos tras la aplicación del enfoque del Aprendizaje Basado en Problemas como un modelo de enseñanza/aprendizaje en el que se combina la adquisición de conocimientos con el desarrollo de habilidades y actitudes útiles para la práctica profesional a través del trabajo en grupos con un tutor para resolver problemas propios de la profesiónS

Geoheritage as an educational resource and educational resources as heritage

El presente trabajo muestra una compilación de experiencias didácticas que emplean el patrimonio geológico como herramienta pedagógica en la enseñanza de las materias relacionadas con las Ciencias de la Tierra. El objetivo de todas ellas es acercar al alumnado al conocimiento y comprensión de los procesos que acontecen en nuestro planeta, con la finalidad de lograr un acercamiento y sensibilización hacia los mismos. Las seis propuestas seleccionadas presentan variedad en cuanto al contexto territorial de desarrollo de las actividades, a los contenidos y niveles trabajados (desde Enseñanza Secundaria hasta estudios universitarios) y a las metodologías utilizadas para la transmisión de dichos contenidos. Dentro de esta diversidad pueden distinguirse dos grupos de actividades diferentes. El primero de ellos se basa en el desarrollo de experiencias pedagógicas en localidades cuyas características geológicas resultan modélicas, únicas y/o espectaculares. Por el contrario, el segundo conjunto de actividades se centra en el trabajo de los recursos geológicos presentes en el entorno próximo del centro educativo. En ellas se explota su valor patrimonial, es decir, su potencial didáctico para abordar el estudio de la historia geológica del territorio. A través de las actividades incluidas en este compendio, se evidencia la validez de ambos tipos de aproximaciones y, al mismo tiempo, se debaten y realzan algunas de las virtudes más destacables de cada una de ellas. De esta manera, este catálogo podría resultar útil e inspirador para posibles planteamientos futuros.This manuscript presents a compilation of didactic experiences, which address the teaching of Earth Sciences through the knowledge of geological heritage. The aim of these proposals is to promote students’ comprehension of the Earth processes so that they may develop a positive attitude towards them. The six activities chosen offer a catalogue with a variety of sites, methodologies, contents and levels. Within this variety, the activities shown can be gathered into two separate groups. On the one hand, the first group collects experiences, which use geological heritage, i.e., the experiences are developed in places whose geological content is exemplary, unique and/or spectacular. On the other hand, the second group is focused on the geologic resources in the neighborhood of the school. These sites have heritage value, that is, an educational value for studying the geologic history of their territory. The activities drawn together show that both viewpoints are useful, and the benefits of each are pointed out. Consequently, this catalogue might be useful and inspiring for forthcoming attempts in this field

Preparación de materiales para la elaboración del trabajo fin de grado

Memoria ID-0144. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2013-2014

p73 regulates ependymal planar cell polarity by modulating actin and microtubule cytoskeleton

[EN]Planar cell polarity (PCP) and intercellular junctional complexes establish tissue structure and coordinated behaviors across epithelial sheets. In multiciliated ependymal cells, rotational and translational PCP coordinate cilia beating and direct cerebrospinal fluid circulation. Thus, PCP disruption results in ciliopathies and hydrocephalus. PCP establishment depends on the polarization of cytoskeleton and requires the asymmetric localization of core and global regulatory modules, including membrane proteins like Vangl1/2 or Frizzled. We analyzed the subcellular localization of select proteins that make up these modules in ependymal cells and the effect of Trp73 loss on their localization. We identify a novel function of the Trp73 tumor suppressor gene, the TAp73 isoform in particular, as an essential regulator of PCP through the modulation of actin and microtubule cytoskeleton dynamics, demonstrating that Trp73 is a key player in the organization of ependymal ciliated epithelia. Mechanistically, we show that p73 regulates translational PCP and actin dynamics through TAp73-dependent modulation of non-musclemyosin-II activity. In addition, TAp73 is required for the asymmetric localization of PCP-core and global signaling modules and regulates polarized microtubule dynamics, which in turn set up the rotational PCP. Therefore, TAp73 modulates, directly and/or indirectly, transcriptional programs regulating actin and microtubules dynamics and Golgi organization signaling pathways. These results shed light into the mechanism of ependymal cell planar polarization and reveal p73 as an epithelial architect during development regulating the cellular cytoskeletonSIThis work was supported by Grants SAF2015-71381-R from Spanish Ministerio de Economía y Competitividad co-financed by FEDER funds (to M.C.M.) and LE021P17 from Junta de Castilla y Leon, and from the Queen Elisabeth Medical Foundation to F.T. J.V.-F. and S.F.-A. are holders of predoctoral fellowships from the Junta de Castilla y León. L.M.-A. is supported by a predoctoral scholarship from the Asociación Española contra el Cáncer (AECC). F.T. is a Research Director of the FNRS. M.W. and M.L. are funded by the Deutsche Forschungsgemeinschaft (DFG) under grant number LI 2405/

p73 is required for appropriate BMP-induced mesenchymal-to-epithelial transition during somatic cell reprogramming

[EN] The generation of induced pluripotent stem cells (iPSCs) by somatic cell reprogramming holds great potential for modeling human diseases. However, the reprogramming process remains very inefficient and a better understanding of its basic biology is required. The mesenchymal-to-epithelial transition (MET) has been recognized as a crucial step for the successful reprogramming of fibroblasts into iPSCs. It has been reported that the p53 tumor suppressor gene acts as a barrier of this process, while its homolog p63 acts as an enabling factor. In this regard, the information concerning the role of the third homolog, p73, during cell reprogramming is limited. Here, we derive total Trp73 knockout mouse embryonic fibroblasts, with or without Trp53, and examine their reprogramming capacity. We show that p73 is required for effective reprogramming by the Yamanaka factors, even in the absence of p53. Lack of p73 affects the early stages of reprogramming, impairing the MET and resulting in altered maturation and stabilization phases. Accordingly, the obtained p73-deficient iPSCs have a defective epithelial phenotype and alterations in the expression of pluripotency markers. We demonstrate that p73 deficiency impairs the MET, at least in part, by hindering BMP pathway activation. We report that p73 is a positive modulator of the BMP circuit, enhancing its activation by DNp73 repression of the Smad6 promoter. Collectively, these findings provide mechanistic insight into the MET process, proposing p73 as an enhancer of MET during cellular reprogramming.S

Dyslipidemia and inflammation as hallmarks of oxidative stress in COVID-19: a follow-up study

Producción CientíficaRecent works have demonstrated a significant reduction in cholesterol levels and increased oxidative stress in patients with coronavirus disease 2019 (COVID-19). The cause of this alteration is not well known. This study aimed to comprehensively evaluate their possible association during the evolution of COVID-19. This is an observational prospective study. The primary endpoint was to analyze the association between lipid peroxidation, lipid, and inflammatory profiles in COVID-19 patients. A multivariate regression analysis was employed. The secondary endpoint included the long-term follow-up of lipid profiles. COVID-19 patients presented significantly lower values in their lipid profile (total, low, and high-density lipoprotein cholesterol) with greater oxidative stress and inflammatory response compared to the healthy controls. Lipid peroxidation was the unique oxidative parameter with a significant association with the total cholesterol (OR: 0.982; 95% CI: 0.969–0.996; p = 0.012), IL1-RA (OR: 0.999; 95% CI: 0.998–0.999; p = 0.021) IL-6 (OR: 1.062; 95% CI: 1.017–1.110; p = 0.007), IL-7 (OR: 0.653; 95% CI: 0.433–0.986; p = 0.042) and IL-17 (OR: 1.098; 95% CI: 1.010–1.193; p = 0.028). Lipid abnormalities recovered after the initial insult during long-term follow-up (IQR 514 days); however, those with high LPO levels at hospital admission had, during long-term follow-up, an atherogenic lipid profile. Our study suggests that oxidative stress in COVID-19 is associated with derangements of the lipid profile and inflammation. Survivors experienced a recovery in their lipid profiles during long-term follow-up, but those with stronger oxidative responses had an atherogenic lipid profile.Instituto de Salud Carlos III - (grant CB21/13/00051 and COV20/00491)Junta de Castilla y León - (grant 18IGOF

Genetic Study of SARS-CoV-2 Non Structural Protein 12 in COVID-19 Patients Non Responders to Remdesivir

Remdesivir (RDV) was the first antiviral drug approved by the FDA to treat severe coronavirus disease-2019 (COVID-19) patients. RDV inhibits SARS-CoV-2 replication by stalling the non structural protein 12 (nsp12) subunit of the RNA-dependent RNA polymerase (RdRp). No evidence of global widespread RDV-resistance mutations has been reported, however, defining genetic pathways to RDV resistance and determining emergent mutations prior and subsequent antiviral therapy in clinical settings is necessary. This study identified 57/149 (38.3%) patients who did not respond to one course (5-days) (n = 36/111, 32.4%) or prolonged (5 to 20 days) (n = 21/38, 55.3%) RDV therapy by subgenomic RNA detection. Genetic variants in the nsp12 gene were detected in 29/49 (59.2%) non responder patients by Illumina sequencing, including the de novo E83D mutation that emerged in an immunosuppressed patient after receiving 10 + 8 days of RDV, and the L838I detected at baseline and/or after prolonged RDV treatment in 9/49 (18.4%) non responder subjects. Although 3D protein modeling predicted no interference with RDV, the amino acid substitutions detected in the nsp12 involved changes on the electrostatic outer surface and in secondary structures that may alter antiviral response. It is important for health surveillance to study potential mutations associated with drug resistance as well as the benefit of RDV retreatment, especially in immunosuppressed patients and in those with persistent replication. IMPORTANCE This study provides clinical and microbiologic data of an extended population of hospitalized patients for COVID-19 pneumonia who experienced treatment failure, detected by the presence of subgenomic RNA (sgRNA). The genetic variants found in the nsp12 pharmacological target of RDV bring into focus the importance of monitoring emergent mutations, one of the objectives of the World Health Organization (WHO) for health surveillance. These mutations become even more crucial as RDV keeps being prescribed and new molecules are being repurposed for the treatment of COVID-19. The present article offers new perspectives for the clinical management of non responder patients treated and retreated with RDV and emphasizes the need of further research of the benefit of combinatorial therapies and RDV retreatment, especially in immunosuppressed patients with persistent replication after therapy.This work was financed by a Gilead Sciences grant (IN-ES-540-6089) and CIBER Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, España (CB21/13/00081). This work was financed by ad hoc patronage funds for research on COVID-19 from donations from citizens and organizations to the Hospital Clínic de Barcelona-Fundació Clínic per a la Recerca Biomèdica.S

Use of eltrombopag for patients 65 years old or older with immune thrombocytopenia

Background Eltrombopag is useful for immune thrombocytopenia (ITP). However, results of clinical trials may not accurately mirror clinical practice reality. Here we evaluated eltrombopag for primary and secondary ITP in our ≥65‐year‐old population. Methods A total of 106 primary ITP patients (16 with newly diagnosed ITP, 16 with persistent ITP, and 74 with chronic ITP) and 39 secondary ITP patients (20 with ITP secondary to immune disorders, 7 with ITP secondary to infectious diseases, and 12 with ITP secondary to lymphoproliferative disorders [LPD]) were retrospectively evaluated. Results Median age of our cohort was 76 (interquartile range, IQR, 70‐81) years. 75.9% of patients yielded a platelet response including 66.2% complete responders. Median time to platelet response was 14 (IQR, 8‐21) days. Median time on response was 320 (IQR, 147‐526) days. Sixty‐three adverse events (AEs), mainly grade 1‐2, occurred. The most common were hepatobiliary laboratory abnormalities (HBLAs) and headaches. One transient ischemic attack in a newly diagnosed ITP and two self‐limited pulmonary embolisms in secondary ITP were the only thrombotic events observed. Conclusion Eltrombopag showed efficacy and safety in ITP patients aged ≥65 years with primary and secondary ITP. However, efficacy results in LPD‐ITP were poor. A relatively high number of deaths were observed