Analysis on Evolution Model of Zombie Company under the Absence of Bank Data

In view of the fact that the data of bank loaning is difficult to be collected, this paper innovatively explores the evolution model of zombie companies by text analysis based on the researches of previous papers at home and abroad based on the financial data of zombie companies. Through the relevant researches on 27 zombies collected, the common characteristics of zombies are found out by the grounded theory. According to the relevant models of enterprise life cycle theory, the evolution model of zombie companies is drawn up, and the corresponding feedback loop of system dynamics causality in each link is further found out, so as to explore the evolutionary rules and the reasons of zombie companies, which is helpful for government to further research on zombies, and favorable for the efficient allocation of market resources and the further rapid development of social economy

Which Factors Determine User’s First and Repeat Online Music Listening Respectively? Music Itself, User Itself, or Online Feedback

In the era of Web 2.0, does online feedback mainly dominant online users’ buying behavior, or are user’s own preference and product quality still important? Previous studies paid more attention to the influence of online feedback on users’ online buying behavior, however this paper focuses on how users’ own factors, product quality related factors and online feedback factors together influence a user’s buying behavior, and also how does this effect change as time goes by. Taking online music as our research industry and using the data from Last.fm website, this research shows that users’ preference and product quality are still the two most dominate factors influencing users’ online music listening, while online feedback plays an important role on users’ first listening. It is also found that the different influences of crowds and friends

Precision Higgs physics at the CEPC

The discovery of the Higgs boson with its mass around 125 GeV by the ATLAS and CMS Collaborations marked the beginning of a new era in high energy physics. The Higgs boson will be the subject of extensive studies of the ongoing LHC program. At the same time, lepton collider based Higgs factories have been proposed as a possible next step beyond the LHC, with its main goal to precisely measure the properties of the Higgs boson and probe potential new physics associated with the Higgs boson. The Circular Electron Positron Collider~(CEPC) is one of such proposed Higgs factories. The CEPC is an $e^+e^-$ circular collider proposed by and to be hosted in China. Located in a tunnel of approximately 100~km in circumference, it will operate at a center-of-mass energy of 240~GeV as the Higgs factory. In this paper, we present the first estimates on the precision of the Higgs boson property measurements achievable at the CEPC and discuss implications of these measurements.Comment: 46 pages, 37 figure

Processing of Ice Cloud In-Situ Data Collected by Bulk Water, Scattering, and Imaging Probes: Fundamentals, Uncertainties and Efforts towards Consistency

In-situ observations of cloud properties made by airborne probes play a critical role in ice cloud research through their role in process studies, parameterization development, and evaluation of simulations and remote sensing retrievals. To determine how cloud properties vary with environmental conditions, in-situ data collected during different field projects processed by different groups must be used. However, due to the diverse algorithms and codes that are used to process measurements, it can be challenging to compare the results. Therefore it is vital to understand both the limitations of specific probes and uncertainties introduced by processing algorithms. Since there is currently no universally accepted framework regarding how in-situ measurements should be processed, there is a need for a general reference that describes the most commonly applied algorithms along with their strengths and weaknesses. Methods used to process data from bulk water probes, single particle light scattering spectrometers and cloud imaging probes are reviewed herein, with emphasis on measurements of the ice phase. Particular attention is paid to how uncertainties, caveats and assumptions in processing algorithms affect derived products since there is currently no consensus on the optimal way of analyzing data. Recommendations for improving the analysis and interpretation of in-situ data include the following: establishment of a common reference library of individual processing algorithms; better documentation of assumptions used in these algorithms; development and maintenance of sustainable community software for processing in-situ observations; and more studies that compare different algorithms with the same benchmark data sets

Physical cues enhancing tissue engineering : from cellular commitment to vascular induction

The behaviors of cells, such as survival, proliferation, differentiation, migration, can produce important physical cues, which lead to the occurrence of some important biological processes. In addition, cells are in close contact with extracellular matrix (ECM) in vivo, either continuously or at some important stages of development. ECM is a three-dimensional (3D) protein scaffold and the physical signals from ECM (stiffness and structure) can also affect cell behaviors. In this thesis, we promoted vascularization of tissue constructs and cardiac differentiation of ESCs through manipulation of cell-induced or biomaterial-derived physical signals. We verified the feasibility of using PLGA nanoparticles to label and track ESC status during EB development. The unique cell viability monitoring function of the nanoparticles allowed clear presentation of live cell distribution within EBs, which had high potential to be a powerful tool to screen EBs with comparable sizes. In addition, we successfully imitated some critical aspects of cartilage hypertrophy using in vitro engineered living cartilage templates and osteogenic treatment. Osteogenic treatment induced cartilage hypertrophy and creates channels for vascular invasion in LhCG constructs when implantation in vivo. Hypertrophic transition of LhCG constructs using osteogenic treatment may contribute to the establishment of an in vitro cartilage hypertrophy model that mimic initial stage of endochondral ossification. Besides manipulation of cell behavior-origin physical signals, stiffness and topography of biomaterials were also controlled. Gelatin-dopamine was synthesized for PDMS surface coating. Compared with gelatin, gelatin-dopamine coating reduced hydrophobicity and increased protein adsorption on the PDMS surface. In addition, the gelatin-dopamine coated PDMS surface greatly improved ESC adhesion, proliferation and pluripotency maintenance as compared to gelatin-coated surfaces. ESC-derived EBs also showed increased adhesion and facilitated myocardial differentiation on the gelatin-dopamine coated PDMS surface. These results demonstrated that gelatin-dopamine coating could effectively improve PDMS surface properties for the long-term ESC culture and enhanced ESC myocardial differentiation. Following the establishment of coating strategy, the influence of PDMS substrate stiffness on ESC pluripotency maintenance and cardiac differentiation was further investigated. Different PDMS substrates exhibited different profiles of substratum properties, which affected the behaviors of ESCs and EBs on PDMS surfaces. ESC adhesion and proliferation, as well as EB attachment, were improved on the 40:1 PDMS substrate as compared to the other PDMS substrates. Meanwhile, compared with the other PDMS substrates, ESC pluripotency preservation was improved on the 5:1 PDMS substrate, while the 40:1 PDMS substrate encouraged ESC cardiac differentiation. These results were successfully applied in fabrication of biochips for ESC pluripotency maintenance and cardiac differentiation. The use of this simple surface modification strategy to stabilize the adhesion of ESCs and EB while enhance their pluripotency and differentiation, as well as substrate stiffness optimization, can possibly contribute to the development of a real heart-on-a-chip for high throughput drug screening in the future. Finally, the effects of pore density and pore sizes on hydrogel vascularization were studied both in vitro and in vivo. An increase of pore density greatly enhanced the proliferation and endothelial differentiation of encapsulated EPOCs in Gel-MCG constructs. In particular, compared to MS:Gelatin (1:1) counterpart, MS:Gelatin (2:1) constructs with higher pore density allowed the interconnected vascular network formation within the whole constructs upon the degradation of thinner gel walls among neighboring pores. Extensive vascular invasion was observed within the MS:Gelatin (2:1) constructs when implanted in vivo. These findings demonstrated that the vascularization of Gel-MCG constructs could be improved by increasing cavitary density. The effects of pore sizes on hydrogel vascularization by EPOCs were also evaluated in vitro and in vivo. Differentiation of EPOCs and hydrogel vascularization were promoted by middle pores as compared to large or small pores. When implantation in vivo, hydrogels with large pores induced more red blood cell-containing vessels and vascular tissues than hydrogels with small or middle pores. These results indicated that middle pores promoted hydrogel vascularization in vitro, while hydrogel vascularization in vivo was improved by large pores. These studies revealed the importance of pore densities and pore sizes in hydrogel vascularization both in vitro and in vivo and these findings can help design an optimal pore density and size for hydrogel constructs to enhance their vascularization. In summary, the studies in this thesis prove that physical signals can be utilized to improve performances of tissue engineered constructs. By utilization of physical signals from cells, we develop a new tool to monitor ESC viability during EB development to screen EBs with homogenous sizes and vascularize chondrocyte-derived cartilage templates with hypertrophic induction. Through manipulation of stiffness and topographic cues pf biomaterials, we improve efficiency of ESC cardiac differentiation and hydrogel vascularization.Doctor of Philosophy (SCBE

In situ organ-specific vascularization in tissue engineering

Other than a few avascular tissues, almost all human tissues are connected to the systemic circulation via blood vessels that promote metabolism and function. Accordingly, engineered vascularization is a vital goal in tissue engineering for regenerative medicine. Endothelial cells (ECs) play a central role in vascularization with two significant specificities: physical interfaces between vascular stroma and blood, and phenotypic organ-specificity. Biomaterial scaffolding technologies that address these unique properties of ECs have been developed to promote the vascularization of various engineered tissues, and these have advanced from mimicking vascular architectures ex situ towards promoting spontaneous angiogenic remodeling in situ. Simultaneously, endothelial progenitor cells (EPCs) and organ-specific ECs are attracting more and more attention with the increasing awareness of the diversity of ECs in different organs

Would You Accept Doctor ChatGPT: An Empirical Study Based on the UTAUT Model

Since its introduction, ChatGPT has generated significant interest in many areas in just a few months, including healthcare. Recently, researchers have claimed that it has passed the U.S. medical licensure examination (Kung et. al, 2022). However, few empirical studies have investigated whether ChatGPT would be valued and accepted by public in the healthcare context. To understand the public\u27s willingness to accept Artificial Intelligence-Generated Content (AIGC) applications like ChatGPT in the healthcare sector, this study proposes a model of factors affecting the user acceptance of ChatGPT for healthcare purposes integrating the Unified Theory of Acceptance and Use of Technology (UTAUT) with the Trust Theory, the Perceived Risk Theory, and the Perceived Illness Theory. We will analyze the data collected from questionnaires using Structural Equation Modeling (SEM). The findings of this study will provide insights into the factors affecting the user acceptance of ChatGPT for healthcare services

Murine pluripotent stem cells derived scaffold-free cartilage grafts from a micro-cavitary hydrogel platform

By means of appropriate cell type and scaffold, tissue-engineering approaches aim to construct grafts for cartilage repair. Pluripotent stem cells especially induced pluripotent stem cells (iPSCs) are of promising cell candidates due to the pluripotent plasticity and abundant cell source. We explored three dimensional (3D) culture and chondrogenesis of murine iPSCs (miPSCs) on an alginate-based micro-cavity hydrogel (MCG) platform in pursuit of fabricating synthetic-scaffold-free cartilage grafts. Murine embryonic stem cells (mESCs) were employed in parallel as the control. Chondrogenesis was fulfilled using a consecutive protocol via mesoderm differentiation followed by chondrogenic differentiation; subsequently, miPSC and mESC-seeded constructs were further respectively cultured in chondrocyte culture (CC) medium. Alginate phase in the constructs was then removed to generate a graft only comprised of induced chondrocytic cells and cartilaginous extracellular matrix (ECMs). We found that from the mESC-seeded constructs, formation of intact grafts could be achieved in greater sizes with relatively fewer chondrocytic cells and abundant ECMs; from miPSC-seeded constructs, relatively smaller sized cartilaginous grafts could be formed by cells with chondrocytic phenotype wrapped by abundant and better assembled collagen type II. This study demonstrated successful creation of pluripotent stem cells-derived cartilage/chondroid graft from a 3D MCG interim platform. By the support of materials and methodologies established from this study, particularly given the autologous availability of iPSCs, engineered autologous cartilage engraftment may be potentially fulfilled without relying on the limited and invasive autologous chondrocytes acquisition.MOE (Min. of Education, S’pore