Sequencing by Cyclic Ligation and Cleavage (CycLiC) directly on a microarray captured template

Next generation sequencing methods that can be applied to both the resequencing of whole genomes and to the selective resequencing of specific parts of genomes are needed. We describe (i) a massively scalable biochemistry, Cyclical Ligation and Cleavage (CycLiC) for contiguous base sequencing and (ii) apply it directly to a template captured on a microarray. CycLiC uses four color-coded DNA/RNA chimeric oligonucleotide libraries (OL) to extend a primer, a base at a time, along a template. The cycles comprise the steps: (i) ligation of OLs, (ii) identification of extended base by label detection, and (iii) cleavage to remove label/terminator and undetermined bases. For proof-of-principle, we show that the method conforms to design and that we can read contiguous bases of sequence correctly from a template captured by hybridization from solution to a microarray probe. The method is amenable to massive scale-up, miniaturization and automation. Implementation on a microarray format offers the potential for both selection and sequencing of a large number of genomic regions on a single platform. Because the method uses commonly available reagents it can be developed further by a community of users

Conductive films based on composite polymers containing ionic liquids absorbed on crosslinked polymeric ionic-like liquids (SILLPs)

Polymerization of styrenic monomers containing imidazolium subunits in the presence of crosslinking monomers and using ionic liquids (ILs) as porogenic agents provides composite materials with excellent mechanical properties and displaying conductivities that are in the same order of magnitude than those shown by bulk ILs. This approach allows the use of high crosslinking degrees and low IL-loadings without compromising the required properties of the resulting composites. Besides, no appreciable leaching of the bulk IL component is detected.Financial support by Ministerio de Ciencia e Innovacion (CTQ2011-28903-C02-01 and SP-ENE-20120718), Generalitat Valenciana (PROMETEO/2012/020) and Universitat Jaume I (P11B2013-38) is acknowledged.Altava Benito, B.; Compañ Moreno, V.; Andrio Balado, A.; Del Castillo Davila, LF.; Mollá Romano, S.; Burguete, MI.; García-Verdugo Cepeda, E.... (2015). Conductive films based on composite polymers containing ionic liquids absorbed on crosslinked polymeric ionic-like liquids (SILLPs). Polymer. 72:69-81. https://doi.org/10.1016/j.polymer.2015.07.009S69817

Soft Ionization of Thermally Evaporated Hypergolic Ionic Liquid Aerosols

Isolated ion pairs of a conventional ionic liquid, 1-Ethyl-3-Methyl-Imidazolium Bis(trifluoromethylsulfonyl)imide ([Emim+][Tf2N?]), and a reactive hypergolic ionic liquid, 1-Butyl-3-Methyl-Imidazolium Dicyanamide ([Bmim+][Dca?]), are generated by vaporizing ionic liquid submicron aerosol particles for the first time; the vaporized species are investigated by dissociative ionization with tunable vacuum ultraviolet (VUV) light, exhibiting clear intact cations, Emim+ and Bmim+, presumably originating from intact ion pairs. Mass spectra of ion pair vapor from an effusive source of the hypergolic ionic liquid show substantial reactive decomposition due to the internal energy of the molecules emanating from the source. Photoionization efficiency curves in the near threshold ionization region of isolated ion pairs of [Emim+][Tf2N?]ionic liquid vapor are compared for an aerosol source and an effusive source, revealing changes in the appearance energy due to the amount of internal energy in the ion pairs. The aerosol source has a shift to higher threshold energy (~;;0.3 eV), attributed to reduced internal energy of the isolated ion pairs. The method of ionic liquid submicron aerosol particle vaporization, for reactive ionic liquids such as hypergolic species, is a convenient, thermally ?cooler? source of isolated intact ion pairs in the gas phase compared to effusive sources

Polymer sieving matrices in microanalytical electrophoresis

Microfluidic design has advanced existing protein separation capabilities and supported novel assays. Key metrics for successful protein separations include fast, robust, and sensitive analysis of complex mixtures of bio-macromolecules. Attaining high separation resolution is a chief concern. Here we review recent advances in polymer-based electrophoresis sieving materials that are impacting microfluidic bioanalytical applications. Looking forward, we comment on unmet needs for advanced separation media in micro-to-nanoscale devices