Aproximación Proteómica al estudio de las proteínas de membrana expresadas por Botrytis cinerea bajo inducción de patogenicidad

Comunicaciones a congreso

El reto de la destreza del speaking en centros de educación secundaria monolingües de Andalucía

Spain, following the recommendations of the Common European Framework of Reference for Languages (CEFR, 2011), is undergoing a quick English-learning process in order to qualify the population as citizens of the world. The mastering of speaking skills in English is crucial to ease personal, social, political, professional and economic relationships. For this reason, the objectives of this paper are: analysing the current regional and national legislation regarding foreign language communication; understanding how relevant it is for the Andalusian population to speak fluently in English, given the magnitude of the international tourism industry in Andalusia and the current European socio-educational context; as well as knowing if Andalusian Secondary Education students are aware of this situation, and how comfortable they feel with their current oral-skills in English nowadays.España, siguiendo las recomendaciones del Marco Común Europeo de Referencia para las Lenguas (MCER, 2011), se encuentra inmersa en un rápido proceso de aprendizaje del inglés que permita a sus gentes convertirse en ciudadanos del mundo. El dominio del componente oral de esta lengua es fundamental para facilitar las relaciones sociales, laborales, políticas o económicas. Por ello, esta investigación establece los siguientes objetivos: analizar la legislación estatal y autonómica vigente con respecto a la comunicación en lenguas extranjeras; entender hasta qué punto es relevante para la población andaluza el hecho de poder hablar con fluidez en lengua inglesa dada la envergadura del turismo internacional en Andalucía y el contexto socioeducativo europeo actual; tratar de averiguar si los estudiantes de Educación Secundaria andaluces son conscientes de esta situación, y conocer cómo de cómodos se sienten hoy día con la práctica de la destreza oral o speaking en lengua inglesa

Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation

Protein phosphorylation and membrane proteins play an important role in the infection of plants by phytopathogenic fungi, given their involvement in signal transduction cascades. Botrytis cinerea is a well-studied necrotrophic fungus taken as a model organism in fungal plant pathology, given its broad host range and adverse economic impact. To elucidate relevant events during infection, several proteomics analyses have been performed in B. cinerea, but they cover only 10% of the total proteins predicted in the genome database of this fungus. To increase coverage, we analysed by LC-MS/MS the first-reported overlapped proteome in phytopathogenic fungi, the “phosphomembranome” of B. cinerea, combining the two most important signal transduction subproteomes. Of the 1112 membrane-associated phosphoproteins identified, 64 and 243 were classified as exclusively identified or overexpressed under glucose and deproteinized tomato cell wall conditions, respectively. Seven proteins were found under both conditions, but these presented a specific phosphorylation pattern, so they were considered as exclusively identified or overexpressed proteins. From bioinformatics analysis, those differences in the membrane-associated phosphoproteins composition were associated with various processes, including pyruvate metabolism, unfolded protein response, oxidative stress response, autophagy and cell death. Our results suggest these proteins play a significant role in the B. cinerea pathogenic cycl

Análisis diferencial del proteoma de Saccharomyces bayanus var. uvarum inducido por la temperatura. Implicación en la calidad de los tintos D.O. Ribera del Duero

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New Perspectives Related to the Bioluminescent System in Dinoflagellates: Pyrocystis lunula, a Case Study

Pyrocystis lunula is considered a model organism due to its bioluminescence capacity linked to circadian rhythms. The mechanisms underlying the bioluminescent phenomenon have been well characterized in dinoflagellates; however, there are still some aspects that remain an enigma. Such is the case of the presence and diversity of the luciferin-binding protein (LBP), as well as the synthesis process of luciferin. Here we carry out a review of the literature in relation to the molecular players responsible for bioluminescence in dinoflagellates, with particular interest in P. lunula. We also carried out a phylogenetic analysis of the conservation of protein sequence, structure and evolutionary pattern of these key players. The basic structure of the luciferase (LCF) is quite conserved among the sequences reported to date for dinoflagellate species, but not in the case of the LBP, which has proven to be more variable in terms of sequence and structure. In the case of luciferin, its synthesis has been shown to be complex process with more than one metabolic pathway involved. The glutathione S-transferase (GST) and the P630 or blue compound, seem to be involved in this process. In the same way, various hypotheses regarding the role of bioluminescence in dinoflagellates are exposed

Development of the “Applied Proteomics” Concept for Biotechnology Applications in Microalgae: Example of the Proteome Data in Nannochloropsis gaditana

Most of the marine ecosystems on our planet are still unknown. Among these ecosystems, microalgae act as a baseline due to their role as primary producers. The estimated millions of species of these microorganisms represent an almost infinite source of potentially active biocomponents offering unlimited biotechnology applications. This review considers current research in microalgae using the “omics” approach, which today is probably the most important biotechnology tool. These techniques enable us to obtain a large volume of data from a single experiment. The specific focus of this review is proteomics as a technique capable of generating a large volume of interesting information in a single proteomics assay, and particularly the concept of applied proteomics. As an example, this concept has been applied to the study of Nannochloropsis gaditana, in which proteomics data generated are transformed into information of high commercial value by identifying proteins with direct applications in the biomedical and agri-food fields, such as the protein designated UCA01 which presents antitumor activity, obtained from N. gaditana

Proteomic approach to Botrytis cinerea survival structures

Comunicaciones a congreso

Applying proteomic technology to dissect molecular aspect of phytopathogenic fungi, a Botrytis cinerea approach

Comunicaciones a congreso

Development of Proteomics-Based Fungicides: New Strategies for Environmentally Friendly Control of Fungal Plant Diseases

Proteomics has become one of the most relevant high-throughput technologies. Several approaches have been used for studying, for example, tumor development, biomarker discovery, or microbiology. In this “post-genomic” era, the relevance of these studies has been highlighted as the phenotypes determined by the proteins and not by the genotypes encoding them that is responsible for the final phenotypes. One of the most interesting outcomes of these technologies is the design of new drugs, due to the discovery of new disease factors that may be candidates for new therapeutic targets. To our knowledge, no commercial fungicides have been developed from targeted molecular research, this review will shed some light on future prospects. We will summarize previous research efforts and discuss future innovations, focused on the fight against one of the main agents causing a devastating crops disease, fungal phytopathogens