PRÁCTICA DE LA ENFERMERA SEGÚN LA TEORÍA PRINCIPIANTE A EXPERTA DEL SERVICIO DE EMERGENCIA DEL HOSPITAL REGIONAL DOCENTE LAS MERCEDES, CHICLAYO- 2018

El objetivo fue, Conocer el nivel de práctica que desarrolla la enfermera según la teoría principiante a experta del servicio de emergencia del Hospital Regional Docente las Mercedes de Chiclayo-2018. La investigación es cuantitativa, del tipo descriptiva, diseño descriptivo simple; la recopilación de datos de se hizo con la técnica de encuesta, aplicada a 50 enfermeras del servicio de emergencia de mencionado hospital. Los resultados más relevantes del perfil socio-demográfico, indican que la mayoría de las enfermeras (88%) sus edades fluctúan entre 25-42 años; el sexo predominante es el femenino (96%); el 86% tienen menos de 18 años como tiempo de servicio; sin embargo la mayoría (98%) de ellas(os) han hecho una especialización; no obstante más de la mitad de ellos(as) su situación laboral es de contrato. En cuanto a las prácticas de las enfermeras en las Dimensiones: Enfermera Principiante, el 58% desarrollo buena práctica, y el 6% logró muy buena práctica; Enfermera Principiante Avanzada, del 64% de ellas fue buena y del 36% muy buena; Enfermera Competente, 70% catalogaron sus prácticas como buenas y 30% como muy buenas; Enfermera Eficiente, el 58% asumieron que fue buena y el 42% muy buena; Enfermería Experta, el 76% consideró su práctica como buena y el 24% como muy buena. Por su parte las medias aritméticas reportan puntaciones similares en las tres dimensiones intermedias con valores que fluctúan entre 40,80-40,96 puntos. Por otro lado la correlación de Pearson (r=-0,22) determino que no existe ningún grado de asociación entre los resultados obtenidos en las diferentes dimensiones.Tesi

Campaña de lanzamiento para flying tiger copenhagen en Perú

El presente trabajo busca elaborar una campaña de lanzamiento para el ingreso de cuatro tiendas de la marca Flying Tiger Copenhagen en el Perú. La propuesta se enfoca en una campaña de lanzamiento en medios digitales, ATL y BTL. La campaña busca comunicar la personalidad de la marca que es divertida y minimalista, resaltar los diseños innovadores de los productos creados por la propia marca y la apertura de cuatro grandes tiendas en el país; con el objetivo de lograr conocimiento y recordación de la marca en los primeros tres meses. Primero, desarrollamos la historia y presencia de la marca ya presente internacionalmente, la evolución de su identidad gráfica y el impacto de las tiendas minoristas de bajo costo. Luego, se definió el público objetivo, nuestra propuesta de posicionamiento y el plan de comunicaciones. Este último consta de estrategías para el público objetivo en redes sociales, web, paneles publicitarios, pauta en televisión y activaciones considerando la locación de las tiendas. Las acciones propuestas se detallaron en un cronograma con presupuesto para cada acción de la campaña.The present work seeks to develop a launch campaign for the entry of four stores of the brand Flying Tiger Copenhagen in Peru. The proposal focuses on an advertising campaign that includes digital media, ATL y BTL. The campaign seeks to communicate the personality of the brand that is fun and minimalist, highlighting the innovative product designs created by the brand itself and the opening of four large stores in the country; with the aim of achieving brand awareness and recall in the first three months. First, we develop the history and presence of the brand already present internationally, the evolution of its graphic identity and the impact of low-cost retail stores were analyzed. Then, the target audience, our positioning proposal and the communications plan were defined. The communication plan consists of strategies for the target audience in social networks, web, advertising panels, television advertising and activations considering the location of the stores. The proposed actions were detailed in a schedule with a budget for each campaign action

El marketing relacional y su influencia en la lealtad de los clientes del restaurante La Vaca, Trujillo 2023

La presente investigación titulada “Marketing relacional y su influencia en la lealtad de los clientes del Restaurante La Vaca, Trujillo 2023”, tuvo como objetivo general, determinar de qué manera el marketing relacional influencia la lealtad de los clientes. El enfoque del estudio fue cuantitativo, con un diseño no experimental y con un nivel explicativo-casual. Asimismo, para el desarrollo, se utilizó como instrumento de recolección de datos un cuestionario con 31 preguntas en total, y para la determinación de la muestra se sacó la fórmula de población finita al 95% de confiabilidad, arrojando a 152 clientes por encuestar. También, se tuvo como apoyo investigaciones de revistas científicas y bibliotecas virtuales. Se alcanzó como resultados, que sí existe influencia del marketing relacional en la lealtad de los clientes del Restaurante La Vaca, Trujillo 2023, con una T estadística mayor a 2 y un valor p de 0,01. De igual manera, se confirma que con respecto a las dimensiones (experiencia, calidad y satisfacción), si existe influencia en la variable lealtad del cliente. Finalmente se concluyó que, al mejorar los esfuerzos en el marketing relacional, se puede mejorar la lealtad de los clientes

Myticalins: A novel multigenic family of linear, cationic antimicrobial peptides from marine mussels (Mytilus spp.)

The application of high-throughput sequencing technologies to non-model organisms has brought new opportunities for the identification of bioactive peptides from genomes and transcriptomes. From this point of view, marine invertebrates represent a potentially rich, yet largely unexplored resource for de novo discovery due to their adaptation to diverse challenging habitats. Bioinformatics analyses of available genomic and transcriptomic data allowed us to identify myticalins, a novel family of antimicrobial peptides (AMPs) from the mussel Mytilus galloprovincialis, and a similar family of AMPs from Modiolus spp., named modiocalins. Their coding sequence encompasses two conserved N-terminal (signal peptide) and C-terminal (propeptide) regions and a hypervariable central cationic region corresponding to the mature peptide. Myticalins are taxonomically restricted to Mytiloida and they can be classified into four subfamilies. These AMPs are subject to considerable interindividual sequence variability and possibly to presence/absence variation. Functional assays performed on selected members of this family indicate a remarkable tissue-specific expression (in gills) and broad spectrum of activity against both Gram-positive and Gram-negative bacteria. Overall, we present the first linear AMPs ever described in marine mussels and confirm the great potential of bioinformatics tools for the de novo discovery of bioactive peptides in non-model organisms

Anti Transglutaminase Antibodies Cause Ataxia in Mice

Background: Celiac disease (CD) is an autoimmune gastrointestinal disorder characterized by the presence of anti-transglutaminase 2 (TG2) and anti-gliadin antibodies. Amongst the neurological dysfunctions associated with CD, ataxia represents the most common one. Methods: We analyzed by immunohistochemistry, the anti-neural reactivity of the serum from 20 CD patients. To determine the role of anti-TG2 antibodies in ataxia, two anti-TG2 single chain variable fragments (scFv), isolated from a phage-display IgA antibody library, were characterized by immunohistochemistry and ELISA, and injected in mice to study their effects on motor coordination. We found that 75% of the CD patient population without evidence of neurological involvement, has circulating anti-neural IgA and/or IgG antibodies. Two anti-TG2 scFvs, cloned from one CD patient, stained blood vessels but only one reacted with neurons. This anti-TG2 antibody showed cross reactivity with the transglutaminase isozymes TG3 and TG6. Intraventricular injection of the anti-TG2 or the anti-TG2/3/6 cross-reactive scFv provoked transient, equally intensive ataxia in mice. Conclusion: The serum from CD patients contains anti-TG2, TG3 and TG6 antibodies that may potentially cause ataxia

Construction of miniantibodies for the in vivo study of human autoimmune diseases in animal models

<p>Abstract</p> <p>Background</p> <p>Phage display antibody libraries have been made from the lymphocytes of patients suffering from autoimmune diseases in which the antibodies are known to play a role in the pathogenesis or are important for the diagnosis of the disease. In the case of Celiac Disease, the immune response is directed against the autoantigen tissue transglutaminase. However, despite numerous studies, the role of these antibodies in the pathogenesis of this disease has not been elucidated.</p> <p>Results</p> <p>We were able to engineer specific anti-transglutaminase antibody fragments in the form called "miniantibody". These are produced by genetic fusion of anti-tTG scFv to Human, Mouse or Rat Fc domains, making them suitable for in vivo expression. The results obtained here indicate that the miniantibody molecule is efficiently secreted, and that the reactivity to the antigen is retained even after fusion to heterologous Fc domains. Further analysis demonstrate that the molecule is secreted as homodimeric, mimicking original antibody structure. Finally, the in vivo expression in mice leads to detectable serum levels with no apparent gross immune response by the host.</p> <p>Conclusion</p> <p>In this work we demonstrated the usefulness of a method for the in vivo expression of miniantibodies specific to transglutaminase, corresponding to the autoimmune specificity of Celiac Disease. This can be proposed as a general method to study the pathogenic role of autoimmune antibodies in autoimmune diseases.</p

Characterization of the Anti-Tissue Transglutaminase Antibody Response in Nonobese Diabetic Mice

Abstract Type 1 diabetes mellitus is an autoimmune disorder characterized by destruction of insulin-producing pancreatic β cells by T lymphocytes. In nonobese diabetic (NOD) mice, a role has been hypothesized for dietary gluten proteins in the onset of diabetes, and because gluten dependence is the major feature of celiac disease, together with production of Abs to the autoantigen tissue transglutaminase (tTG), we looked for the presence of anti-tTG Abs in the serum of NOD mice and, to establish their origin, analyzed the Ab repertoire of NOD mice using phage display Ab libraries. We found significant levels of serum anti-tTG Abs and were able to isolate single-chain Ab fragments to mouse tTG mainly from the Ab libraries made from intestinal lymphocytes and to a lesser extent from splenocytes. Data from NOD mice on a gluten-free diet suggest that the anti-tTG response is not gluten-dependent. The intestinal Ab response to tTG is a feature of NOD mice, but the underlying mechanisms remain obscure

Lack of Accredited Clinical Training in Movement Disorders in Europe, Egypt, and Tunisia

Background: Little information is available on the official postgraduate and subspecialty training programs in movement disorders (MD) in Europe and North Africa. Objective: To survey the accessible MD clinical training in these regions. Methods: We designed a survey on clinical training in MD in different medical fields, at postgraduate and specialized levels. We assessed the characteristics of the participants and the facilities for MD care in their respective countries. We examined whether there are structured, or even accredited postgraduate, or subspecialty MD training programs in neurology, neurosurgery, internal medicine, geriatrics, neuroradiology, neuropediatrics, and general practice. Participants also shared their suggestions and needs. Results: The survey was completed in 31/49 countries. Structured postgraduate MD programs in neurology exist in 20 countries; structured neurology subspecialty training exists in 14 countries and is being developed in two additional countries. Certified neurology subspecialty training was reported to exist in 7 countries. Recommended reading lists, printed books, and other materials are the most popular educational tools, while courses, lectures, webinars, and case presentations are the most popular learning formats. Mandatory activities and skills to be certified were not defined in 15/31 countries. Most participants expressed their need for a mandatory postgraduate MD program and for certified MD sub-specialization programs in neurology. Conclusion: Certified postgraduate and subspecialty training exists only in a minority of European countries and was not found in the surveyed Egypt and Tunisia. MD training should be improved in many countries.Peer reviewe

Developmental stages and gut microenvironments influence gut microbiota dynamics in the invasive beetle Popillia japonica Newman (Coleoptera: Scarabaeidae)

Popillia japonica Newman (Coleoptera: Scarabaeidae) is a highly polyphagous invasive beetle originating from Japan. This insect is highly resilient and able to rapidly adapt to new vegetation. Insect?associated microorganisms can play important roles in insect physiology, helping their hosts to adapt to changing conditions and potentially contributing to an insect's invasive potential. Such symbiotic bacteria can be part of a core microbiota that is stably transmitted throughout the host's life cycle or selectively recruited from the environment at each developmental stage. The aim of this study was to investigate the origin, stability and turnover of the bacterial communities associated with an invasive population of P. japonica from Italy. Our results demonstrate that soil microbes represent an important source of gut bacteria for P. japonica larvae, but as the insect develops, its gut microbiota richness and diversity decreased substantially, paralleled by changes in community composition. Notably, only 16.75% of the soil bacteria present in larvae are maintained until the adult stage. We further identified the micro?environments of different gut sections as an important factor shaping microbiota composition in this species, likely due to differences in pH, oxygen availability and redox potential. In addition, P. japonica also harboured a stable bacterial community across all developmental stages, consisting of taxa well known for the degradation of plant material, namely the families Ruminococcacae, Christensenellaceae and Lachnospiraceae. Interestingly, the family Christensenallaceae had so far been observed exclusively in humans. However, the Christensenellaceae operational taxonomic units found in P. japonica belong to different taxonomic clades within this family