Antibiotic resistance of Helicobacter pylori: a cross-sectional study in consecutive patients, and relation to ethnicity

ObjectivesTo assess primary antibiotic resistance in a given population and relate the results to ethnicity.Materials and methodsConsecutive cultures were tested for antibiotic susceptibility with the Etest. Three populations were studied separately: ethnic Dutch people, patients of Turkish descent, and patients originating from Africa and the Middle East.ResultsOver a period of 5.5 years, 976 (32%) biopsy specimens from 3010 patients were positive for Helicobacter pylori. Metronidazole and clarithromycin resistance were present in 25.8% and 4.8% of the strains, respectively. The number of metronidazole-resistant strains showed a gradual decrease, while clarithromycin resistance showed a slight increase during the study period. Antimicrobial resistance in patients of Turkish descent and in those originating from Africa or the Middle East was significantly higher than in ethnic Dutch people, 35% and 9.1% versus 21% and 2.9%, respectively (P = 0.003 and P = 0.002).ConclusionIt is important to take ethnicity into account when studying antibiotic resistance. The numbers of metronidazole- and clarithromycin-resistant strains can vary considerably between people of different ethnic origin living in the same region

Suplemento alimenticio en colonias de abejas para la mejora del servicio de polinización de kiwi (Actinidia deliciosa Liang & Ferguson) (Actinidiaceae: Theales)

El modelo actual de agricultura determina una disminución en los hábitats seminaturales lo que conduce a una mala nutrición de las colonias de abejas, las cuales generalmente necesitan ser suplementadas con alimentos. Las abejas se utilizan para transferir polen entre plantas de kiwi (Actinidia deliciosa Liang & Ferguson masculina y femenina, aumentando así la calidad de la fruta y el rendimiento de los cultivos. El objetivo principal fue determinar el efecto de la estimulación de las colonias de Apis mellifera L. con suministros alimentarios estándar sobre la recolección de polen de kiwi. Los tratamientos (n= 5 colmenas cada uno) se realizaron en un huerto de kiwis en Mar del Plata, Argentina: Grupo J/A: suministrado con jarabe de azúcar (2:1) + suplemento proteico líquido (“Apipromotor®”); Grupo J/P: suministrado con jarabe de azúcar (2:1) + suplemento de proteínas sólidas ("patty"); Grupo J: suministrado con jarabe de azúcar (2:1); Grupo C: control, no suministrado. Las colonias abastecidas con J, J/P y J/A recolectaron más polen de kiwifruit que el tratamiento control, incluso bajo la presencia de otras especies florales en áreas cercanas. Aunque las abejas recolectaron la mayor parte del polen de otras especies de plantas de hábitats seminaturales, los tratamientos bajo estimulación artificial (J/P, J/A y J) pueden mejorar significativamente el servicio de polinización de kiwis que realizan las abejas.The current agriculture model determines a decrease in semi-natural habitats leading to poor nutrition for honeybee colonies, which usually need to be food supplemented. Honeybees are used to transfer pollen between male and female kiwifruit (Actinidia deliciosaLiang & Ferguson) plants, increasing fruit quality and crop yield. Our main goal was to determine the effect of stimulating Apis mellifera L. colonies with standard food supplies on the collection of kiwifruit pollen. However, honey bees can also forage other flowering species in the crop site´s surrounding areas. We selected kiwifruit as a model to analyze the effects of food supply on pollen collection of the target crop. The following experimental treatments (n = 5 hives each were conducted in a kiwifruit orchard in Mar del Plata, Argentina: Group J/A: supplied with sugar syrup (2:1 + liquid protein supplements ("Apipromotor ®"; Group J/P: supplied with sugar syrup (2:1 + solid protein supplements ("patty";Group J: supplied with sugar syrup (2:1; Group C: control, not supplied. Colonies supplied with J, J/P and J/A collected more kiwifruit pollen than the other two treatments, even under other flowering species in areas nearby. Although honeybees collected most pollen from other plant species of semi-natural habitats, J/P, J, and J/A treatments can significantly improve the honeybees´ kiwifruit pollination service.Fil: de Piano, Fiorella Giselle. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Meroi Arcerito, Facundo René. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; ArgentinaFil: De Feudis, Leonardo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Basilio, Alicia M.. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Galetto, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentin

Psicología y entrenadores : una complementariedad imprescindible

Obra ressenyada : J. ALVES y A. Paula BRITO (eds.), Manual de Psicologia do Desporto Para Treinadores. Lisboa : Visões e Contextos, 2011

La protección del nasciturus en el proceso de divorcio

En el proceso de divorcio habiendo hijos no natos es menester el establecimiento de medidas provisionales que fijen una cuantía para satisfacer las necesidades del nasciturus – tales como ecografías, clases de preparación del parto, etc. – así como que tales medidas varíen a lo largo del crecimiento del hijo para adecuarse a las necesidades de cada momento en vez del establecimiento de una cuantía que no varíe con las necesidades, necesitándose en dicho proceso la presencia de un mediador imparcial que permita la viabilidad de la presente teoría

Improving the burden of hereditary angioedema through targeted treatment

Hereditary angioedema (HAE) is a rare autosomal dominant disease, which so far cannot be cured. The aim of this thesis is to describe the full burden of this disease and to contribute to new therapeutic strategies to prevent the recurrence of angioedema attacks. Part A concerns the entire burden of HAE, predominantly focussing on patients in the Netherlands. This burden of HAE comprises of angioedema control, health-related quality of life, the burden of treatment, personal and societal costs, reproductive decisions, precautionary measures before partaking in activities which can trigger angioedema attacks, and the burden that comes with participating in clinical trials. In Part B the current landscape of prophylactic and acute treatment is described, where after the prospective targets of future therapies are discussed. Part B contains the description of the first two angioedema patients who received prekallikrein antisense oligonucleotides, as well as the phase 2 trial with this new treatment in HAE patients. This is followed by additional laboratory analyses of plasma collected in this phase 2 trial, elucidating whether prekallikrein inhibition causes increased thrombotic risk. Lastly, the phase 2 trial investigating activated factor XII inhibition in HAE patients is discussed

Indoleamine-2,3-dioxygenase activity in experimental human endotoxemia

Background: Excessive tryptophan metabolism to kynurenine by the rate-limiting enzyme endothelial indoleamine 2,3-dioxygenase 1 (IDO) controls arterial vessel relaxation and causes hypotension in murine endotoxemia. However, its relevance in human endotoxemia has not been investigated so far. We thus aimed to study changes in blood pressure in parallel with tryptophan and kynurenine levels during experimental endotoxemia in humans. Findings: Six healthy male volunteers were given E. coli lipopolysaccharide (LPS; 4 ng/kg) as a 1-min intravenous infusion. They had levels of soluble E-Selectin and soluble vascular cell adhesion molecule-1 as well as IDO activity assessed as the kynurenine-to-tryptophan plasma ratio by liquid chromatography-tandem mass spectrometry at various time points during a 24 h time course. During endotoxemia, IDO activity significantly increased, reaching peak levels at 8 h after LPS infusion (44.0 ± 15.2 vs. 29.4 ± 6.8 at baseline, P<0.0001). IDO activity correlated inversely with the development of hypotension as shown by random effects linear regression models. Finally, IDO activity exhibited a kinetic profile similar to that of soluble endothelial-specific adhesion molecules. Conclusions: LPS is a triggering factor for the induction of IDO in men. Our findings strongly support the concept that the induction of IDO in the vascular endothelium contributes to hypotension in human sepsis

NECROTIZING FASCIITIS OF THE POSTERIOR CERVICAL COMPARTMENT: AN ATYPICAL CASE DUE TO STREPTOCOCCUS AGALACTIAE

Necrotizing fasciitis is a fulminant infection that affects the deep and superficial fascia while initially sparing the overlying skin and underlying muscle. The involvement of the cervicalcompartment is associated with a high morbidity and mortality rate. Immunocompromised patients are prone to this infection. We present a case of a 80 year-old diabetic womansuffering from streptococcal cervical NF (probably secondary to a arm injury) with an uncommon involvement of the posterior cervical compartment, highlighting the atypical responsible microorganism (Streptococcus Agalactiae), the role of imaging for early diagnosis and the timely surgical and medical treatment for a successful outcome.

A single-molecule FRET sensor for monitoring DNA synthesis in real time

We developed a versatile DNA assay and framework for monitoring polymerization of DNA in real time and at the single-molecule level

Anatomía no es destino.

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