Discovery of the Optical Transient of the Gamma Ray Burst 990308

The optical transient of the faint Gamma Ray Burst 990308 was detected by the QUEST camera on the Venezuelan 1-m Schmidt telescope starting 3.28 hours after the burst. Our photometry gives $V = 18.32 \pm 0.07$, $R = 18.14 \pm 0.06$, $B = 18.65 \pm 0.23$, and $R = 18.22 \pm 0.05$ for times ranging from 3.28 to 3.47 hours after the burst. The colors correspond to a spectral slope of close to $f_{\nu} \propto \nu^{1/3}$. Within the standard synchrotron fireball model, this requires that the external medium be less dense than $10^{4} cm^{-3}$, the electrons contain $> 20$ of the shock energy, and the magnetic field energy must be less than 24% of the energy in the electrons for normal interstellar or circumstellar densities. We also report upper limits of $V > 12.0$ at 132 s (with LOTIS), $V > 13.4$ from 132-1029s (with LOTIS), $V > 15.3$ at 28.2 min (with Super-LOTIS), and a 8.5 GHz flux of $< 114 \mu Jy$ at 110 days (with the Very Large Array). WIYN 3.5-m and Keck 10-m telescopes reveal this location to be empty of any host galaxy to $R > 25.7$ and $K > 23.3$. The lack of a host galaxy likely implies that it is either substantially subluminous or more distant than a red shift of $\sim 1.2$.Comment: ApJ Lett submitted, 5 pages, 2 figures, no space for 12 coauthor

The Structure-Function Linkage Database

The Structure–Function Linkage Database (SFLD, http://sfld.rbvi.ucsf.edu/) is a manually curated classification resource describing structure–function relationships for functionally diverse enzyme superfamilies. Members of such superfamilies are diverse in their overall reactions yet share a common ancestor and some conserved active site features associated with conserved functional attributes such as a partial reaction. Thus, despite their different functions, members of these superfamilies ‘look alike’, making them easy to misannotate. To address this complexity and enable rational transfer of functional features to unknowns only for those members for which we have sufficient functional information, we subdivide superfamily members into subgroups using sequence information, and lastly into families, sets of enzymes known to catalyze the same reaction using the same mechanistic strategy. Browsing and searching options in the SFLD provide access to all of these levels. The SFLD offers manually curated as well as automatically classified superfamily sets, both accompanied by search and download options for all hierarchical levels. Additional information includes multiple sequence alignments, tab-separated files of functional and other attributes, and sequence similarity networks. The latter provide a new and intuitively powerful way to visualize functional trends mapped to the context of sequence similarity

Human resource allocation management in multiple projects using sociometric techniques

This article describes a new application of key psychological concepts in the area of Sociometry for the selection of workers within organizations in which projects are developed. The project manager can use a new procedure to determine which individuals should be chosen from a given pool of resources and how to combine them into one or several simultaneous groups/projects in order to assure the highest possible overall work efficiency from the standpoint of social interaction. The optimization process was carried out by means of matrix calculations performed using a computer or even manually, and based on a number of new ratios generated ad-hoc and composed on the basis of indices frequently used in Sociometry

TNOs are Cool: A survey of the trans-Neptunian region V. Physical characterization of 18 Plutinos using Herschel PACS observations

We present Herschel PACS photometry of 18 Plutinos and determine sizes and albedos for these objects using thermal modeling. We analyze our results for correlations, draw conclusions on the Plutino size distribution, and compare to earlier results. Flux densities are derived from PACS mini scan-maps using specialized data reduction and photometry methods. In order to improve the quality of our results, we combine our PACS data with existing Spitzer MIPS data where possible, and refine existing absolute magnitudes for the targets. The physical characterization of our sample is done using a thermal model. Uncertainties of the physical parameters are derived using customized Monte Carlo methods. The correlation analysis is performed using a bootstrap Spearman rank analysis. We find the sizes of our Plutinos to range from 150 to 730 km and geometric albedos to vary between 0.04 and 0.28. The average albedo of the sample is 0.08 \pm 0.03, which is comparable to the mean albedo of Centaurs, Jupiter Family comets and other Trans-Neptunian Objects. We were able to calibrate the Plutino size scale for the first time and find the cumulative Plutino size distribution to be best fit using a cumulative power law with q = 2 at sizes ranging from 120-400 km and q = 3 at larger sizes. We revise the bulk density of 1999 TC36 and find a density of 0.64 (+0.15/-0.11) g cm-3. On the basis of a modified Spearman rank analysis technique our Plutino sample appears to be biased with respect to object size but unbiased with respect to albedo. Furthermore, we find biases based on geometrical aspects and color in our sample. There is qualitative evidence that icy Plutinos have higher albedos than the average of the sample.Comment: 18 pages, 8 figures, 8 tables, accepted for publication in A&

Self-organizing actin networks drive sequential endocytic protein recruitment and vesicle release on synthetic lipid bilayers

UNLABELLED: Forces generated by actin assembly assist membrane invagination during clathrin-mediated endocytosis (CME). The sequential recruitment of core endocytic proteins and regulatory proteins, and assembly of the actin network, are well documented in live cells and are highly conserved from yeasts to humans. However, understanding of CME protein self-organization, as well as the biochemical and mechanical principles that underlie actin’s role in CME, is lacking. Here, we show that supported lipid bilayers coated with purified yeast WASP, an endocytic actin assembly regulator, and incubated in cytoplasmic yeast extracts, recruit downstream endocytic proteins and assemble actin tails. Time-lapse imaging of WASP-coated bilayers revealed sequential recruitment of proteins from different endocytic modules, faithfully replicating in vivo behavior. Reconstituted actin networks assemble in a WASP-dependent manner and deform lipid bilayers, as seen by electron microscopy. Time-lapse imaging revealed that vesicles are released from the lipid bilayers with a burst of actin assembly. Actin networks pushing on membranes have previously been reconstituted; here, we have reconstituted a biologically important variation of these actin networks that self-organize on bilayers and produce pulling forces sufficient to bud off membrane vesicles. We propose that actin-driven vesicle generation may represent an ancient evolutionary precursor to diverse vesicle forming processes adapted for a wide array of cellular environments and applications. SIGNIFICANCE STATEMENT: Actin filament assembly participates in many vesicle-forming processes. However, the underlying principles for how assembly is initiated and organized to effectively harness assembly forces remain elusive. To address this gap, we report a novel reconstitution of actin-driven vesicle release from supported lipid bilayers. Using real-time imaging, we observe sequential recruitment of endocytic proteins and, following a burst of actin assembly, vesicle release from bilayers. Given the absence of cargo or upstream endocytic regulatory proteins on the bilayers, and the participation of actin in many vesicle-forming processes, we posit that this mode of vesicle formation represents an early evolutionary precursor for multiple trafficking pathways. We expect that this assay will be of great use for future investigations of actin-mediated vesicle-forming processes

The Gender Gap in Political Interest Revisited

To what extent does conventional survey measurement capture the political interest of men and women equally well? We aim to answer this question by relying on unique data from a national online survey in Spain, where we used various questions unpacking the standard indicator of political interest. The findings show that men and women nominate different personal political interests. We also find that the gender gap in political interest vanishes once these specific interests are taken into account. This suggests that at least part of the documented gender gap in general political interest might be due to the fact that, when prompted to think about politics, women disregard their own specific political interests and instead focus on the dominant, male-oriented understanding of politics